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http://dx.doi.org/10.4014/jmb.1507.07098

Comparative Analysis of Performance and Microbial Characteristics Between High-Solid and Low-Solid Anaerobic Digestion of Sewage Sludge Under Mesophilic Conditions  

Lu, Qin (National Engineering Research Center for Urban Pollution Control, School of Environmental Science and Engineering, Tongji University)
Yi, Jing (National Engineering Research Center for Urban Pollution Control, School of Environmental Science and Engineering, Tongji University)
Yang, Dianhai (National Engineering Research Center for Urban Pollution Control, School of Environmental Science and Engineering, Tongji University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.1, 2016 , pp. 110-119 More about this Journal
Abstract
High-solid anaerobic digestion of sewage sludge achieves highly efficient volatile solid reduction, and production of volatile fatty acid (VFA) and methane compared with conventional low-solid anaerobic digestion. In this study, the potential mechanisms of the better performance in high-solid anaerobic digestion of sewage sludge were investigated by using 454 high-throughput pyrosequencing and real-time PCR to analyze the microbial characteristics in sewage sludge fermentation reactors. The results obtained by 454 highthroughput pyrosequencing revealed that the phyla Chloroflexi, Bacteroidetes, and Firmicutes were the dominant functional microorganisms in high-solid and low-solid anaerobic systems. Meanwhile, the real-time PCR assays showed that high-solid anaerobic digestion significantly increased the number of total bacteria, which enhanced the hydrolysis and acidification of sewage sludge. Further study indicated that the number of total archaea (dominated by Methanosarcina) in a high-solid anaerobic fermentation reactor was also higher than that in a low-solid reactor, resulting in higher VFA consumption and methane production. Hence, the increased key bacteria and methanogenic archaea involved in sewage sludge hydrolysis, acidification, and methanogenesis resulted in the better performance of high-solid anaerobic sewage sludge fermentation.
Keywords
Anaerobic digestion; high solid; sewage sludge; microbial characteristic; pyrosequencing;
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