• 한국수산과학회지
• /
• 제43권6호
• /
• pp.637-641
• /
• 2010

The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as $1,024-2,048\;{\mu}g{\cdot}mL^{-1}$. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative ${\beta}$-lactamase gene, and the MIC for ampicillin of the mutant strain was $1{\mu}g{\cdot}mL^{-1}$. In conclusion, our findings suggest that the VPA0477 gene acts as a ${\beta}$-lactamase in ampicillin-resistant V. parahaemolyticus strains.

• 한국수산과학회지
• /
• 제42권1호
• /
• pp.20-25
• /
• 2009

Thirty-eight Vibrio spp. were isolated from the sea waters harvested from the 22 stations located on the west coast of the Korean peninsula in September 2006. The isolates consisted of V. parahaemolyticus (n=21), V. alginolyticus (n= 16) and V. cholerae non-01 (n=1), among which 35 isolates displayed resistance against two of the tested antibiotics. Among the 38 isolates, 18 isolates exhibited multi-drug resistance against more than four 4 antibiotics. In particular, minimum inhibitory concentration $(MIC)_{50}$ and $MIC_{90}$ of ampicillin-resistant isolates were as high as $2,048{\mu}g{\cdot}mL^{-1}$ and $4,096{\mu}g{\cdot}mL^{-1}$ respectively. $\beta$-lactamase production was examined to analyze the ampicillin-resistance. Some Vibrio spp. isolates produced $\beta$-lactamase, however antibiotics resistance pattern and $\beta$-lactamase production were not clearly related to each other. A genetic relationship between resistance and gene expression was confirmed in the ampicillin-resistant isolates.

• 대한수의학회지
• /
• 제25권1호
• /
• pp.61-67
• /
• 1985

One hundred and twenty seven strains of Gram-negative rods (72 E. coli, 45 Klebsieila pneumoniae, 8 Enterobacter spp. and 2 Pseudomonas aeruginosa) isolated from bovine mastitis were examined for resistance to ampicilin, carbenicillin and cefazolin, ${\beta}$-lactamase activity and transferable ${\beta}$-lactamase plasmids. Stains resistant to ampicillin were 13.9% in E. coli, 93.3% in Klebsiella pneumoniae, 87.5% in Enterobacter. spp. and all in Pseudomonas aeruginosa, Resistance of E. coli, Klebsiella pneumoniae and Enterobacter spp. to ampicillin was due to the ${\beta}$-lactamases, but all Pseudomonas aeruginosa exhibited a high level of the non-enzymic resistance. Transferable plasmid-mediated ${\beta}$-lactamase synthesis was demonstrated in 61.9% of Klebsiella pneumoniae, 50% of E. coli and 42.9% of Enterobacter spp. The same ${\beta}$-lactamase plasmids specified different resistance levels to various ${\beta}$-lactam antibiotics in different recipients.

• 한국가금학회지
• /
• 제7권1호
• /
• pp.53-64
• /
• 1980

A total of 1503 specimens were submitted to the Poultry Disease Diagnostic Service Laboratory during the year 1966 and 1978. The most frequently diagnosed diseases in order of prevalence were avian mycoplasmosis, staphylococcosis, colibacillosis, salmonellosis and pullorum disease, the percentages of the conditions being 24.6%, 20.0%, 18.0%, 12.6% and 6.4%, respectively, The drug resistance of pathogenic mirnoorganisms isolated during the year 1978 from chicken with colicabacillosis, staphylococcosis or salmonellosis were investigated by the use of disc diffusion technique, the results being as follow. 1) Drug resistance of 63 strains of Escherichia coli More than 95% of the strains tested were sensitive to colistin and gentamicin. The percentages of strains sensitive to kanamycin, chloramphenicol, ampicillin and nitrofurantoin were 66.7%, 60.3%, 60.3% and 47.6%, respectively. Majority of the strains were highly resistant to streptomycin and tetracyline. All the strains were resisistant to bacitracin lincomycin, oleandomycin, penicillin and erythromycin. All the strains tested were resistant to more than two among 10 drugs in common use such as penicillin, erythromycin, streptomycin, tetracycline, neomycin, chloramphenicol, kanamycin, ampicillin and gentamicin, and 27 different resistance patterns were noted. The most frequent multiple resistance pattern was PC, EM, SM and TC (11.1%). 2) Drug resistance of 48 strains of Salmonella More than 95% of the strains tested were sensitive to colistin, gentamicin ana ampicillin. The percentages of st rains sensitive to kanamycin, tetracycline, neomycin and nitrofurantoin were 81,3%, 79%, 72.9%, and 68.0% respectively. None of them was sensitive to streptomycin, oleandomycin, erythromycin, lincomycin and bacitracin. All the strains were resistant to more than one among 7 drugs in common use such as streptomycin, erythromycin, neomycin, tetracycline, kanamycin, ampicillin and gentamicin. The most frequent resistance pattern was SM and EM(66.7%). 3) Drug resistance of 54 strains of Staphylococci All the strains tested were sensitive to gentmaicin, kanamycin and cephalothin. Majority of them were highly sensitive to bacitracin, methicillin, nitrofurantoin and chloramphenicol. The Percentages of strains sensitive to streptomycin, ampicillin, lincomycin and tetracycline were 66.7%, 55.6%, 44.4% and 27.8%, respectively. Among them, 51 strains were resistant to more than one among 11 drugs in common use such as tetracycline, lincomycin, ampicillin, penicillin, streptomycin, erythromycin, neomycin, oleandomycin, chloramphenicol, methicillin and bacitracin, and thirty one different resistance patterns were noted.

• 한국식품과학회지
• /
• 제42권4호
• /
• pp.508-513
• /
• 2010

2006년부터 2008년까지 3년간 시판 수산물 2,239건에서 분리한 V. parahaemolyticus에 대한 항생제 감수성 결과는 다음과 같았다. 어류 789건을 검사하여 42주(5.3%)의 V. parahaemolyticus가 분리 동정되었으며, 패류 735건 중 27건(3.7%), 연체류 387건 중 12건(3.1%), 기타 수산물 328건 중 12건(3.7%), 총 93건(4.2%)에서 V. parahaemolyticus가 분리 동정되었다. 내성률이 높은 항생제는 ampicillin 93.5%, cephalothin 90.3%, streptomycin 87.1%, ticarcillin 55.9%, 그리고 amikacin 40.9%이었으며, 감수성률이 높은 항생제는 nalidixic acid, chloramphenicol 및 ampicillin/sulbactam trimethoprim/sulfamethoxazole 98.9%, gentamicin 및 tetracycline 82.8%, ceftriaxone 63.4%이었다. 중등도 내성률은 kanamycin 79.6%, ciprofloxacin 64.5%, amikacin 47.3%, cefoxitin 43.0%순이었다. 항생제 내성양상은 3제 내성이 37.6%, 4제 내성 24.7%, 5제 내성 17.2%, 6제 내성 11.8%, 2제 내성6.5%, 7제 내성 2.2% 순이었으며, 가장 많은 내성 양상은 AM-CF-S의 3제 내성(22.6%), AM-CF-S-TIC 4제 내성(18.3%), AM-AN-CF-S-TIC 5제 내성(9.7%) 순이었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제45권6호
• /
• pp.324-331
• /
• 2019

Objectives: This study investigated the types and antibiotic sensitivity of bacteria in odontogenic abscesses. Materials and Methods: Pus specimens from 1,772 patients were collected from affected areas during incision and drainage, and bacterial cultures and antibiotic sensitivity tests were performed. The number of antibiotic-resistant bacteria was analyzed relative to the total number of bacteria that were tested for antibiotic susceptibility. Results: Bacterial cultures from 1,772 patients showed a total of 2,489 bacterial species, 2,101 gram-positive and 388 gram-negative. For penicillin G susceptibility tests, 2 out of 31 Staphylococcus aureus strains tested showed sensitivity and 29 showed resistance. For ampicillin susceptibility tests, all 11 S. aureus strains tested showed resistance. In ampicillin susceptibility tests, 46 out of 50 Klebsiella pneumoniae subsp. pneumoniae strains tested showed resistance. Conclusion: When treating odontogenic maxillofacial abscesses, it is appropriate to use antibiotics other than penicillin G and ampicillin as the first-line treatment.

• 한국식품영양학회지
• /
• 제26권1호
• /
• pp.132-136
• /
• 2013

Antimicrobial resistance and multi-drug resistance patterns have been carried out on total of 210 isolated of Salmonella spp. and pathogenic E. coli isolated from food poisoning patients on January through December 2012 in Incheon, Korea. The highest percentage of antibiotics resistance was found to the following antimicrobial agents: tetracycline 43.8%, ampicillin 34.8%, nalidixic acid 23.8%, sulfamethoxazole/trimethoprim and chloramphenicol 12.4%, and ampicillin/sulbactam 11.4%. The highest percentage of resistance was 37.5% to ampicillin for Salmonella spp. and 59.0% to tetracycline for pathogenic E. coli. Overall the multidrug resistance rates of 1 drug was 26.2%, 2 drugs 9.0%, 3 drugs 9.5%, 4 drugs 7.1%, and 5 or more drugs 12.46%. The multi-drug (MDR) strains to four or more antimicrobial agents among the resistant organisms were quite high: 15.9% and 22.1% for Salmonella spp. and pathogenic E. coli, respectively. The study implies that limitation of unnecessary medication use is pertinent in order to maintaining the efficacy of drugs.

• 미생물학회지
• /
• 제49권2호
• /
• pp.118-125
• /
• 2013

지난 2000년부터 2009년까지 10년간 광주광역시 지역의 급성설사환자로부터 분리한 총 596개의 살모넬라균주에 대하여 총 16종의 항생제에 대한 감수성 검사를 실시한 결과, 내성빈도가 가장 높았던 약제는 ampicillin (43%), tetracycline (35.9%), nalidixic acid (31.5%), 그리고 chloramphenicol (26.2%) 순으로 나타났다. 혈청형 간에 항생제 내성률에 차이가 있었는데 S. Enteritidis와 S. Typhimurium이 각각 가장 높은 내성률을 보인 약제는 ampicillin (51.1%)과 tetracycline (77.9%)이었다. 총 89가지 내성패턴이 관찰되었으며, 26% (155/596)의 살모넬라 분리주는 검사한 16가지 약제에 모두 감수성을 보였다. 약 21% (127/596) 및 15% (87/596)의 분리주는 각각 한 개 및 두 개의 약제에 내성을 나타냈다. 그 외의 살모넬라 분리주(227/596, 38%)는 세가지 이상의 약제에 내성을 나타냈다(다제내성균). 다제내성균이 가장 높은 비율로 나타난 혈청형은 S. Paratyphi B (76.5%), S. Typhimurium (58.2%), 그리고 S. Enteritidis (40.2%)의 순이었다. 가장 흔한 다제내성패턴은 ampicillin-chloramphenicol-nalidixic acid-ticarcillin (36/227, 15.8%)이었으며 이러한 패턴을 보였던 다제내성균의 대부분(35/36, 97.2%)이 S. Enteritidis이었다.

• 한국동물위생학회지
• /
• 제23권2호
• /
• pp.153-163
• /
• 2000

This study was carried out the antimicrobial resistance of staphylococcus aureus isolated from bovine mastitic milk, chickens, korean native cattle, korean native goats, pigs, dog and mice in northern area of kyongbuk. The result were summarised as follows ; A total of 149 S aureus were isolated from bovine mastitic milk, chickens, korean native cattle, korean native goats, pigs, dog and mice. In 80 isolates of S aureus from bovine mastitic milk, 60% of isolates revealed resistance to penicillin and ampicillin, 19% to gentamicin, 6% to tetracycline. In 36 isolates of S aureus from chickens, 72% of isolates revealed resistance to tetracycline, 58% to penicillin and ampicillin, 42% to streptomycin, 31% to lincomycin, 25% to norfloxacin, 22% to gentamicin. In 17 isolates of S aureus from korean native cattle, 100% of isolates revealed resistance to penicillin and ampicillin, 88% to lincomycin, 76% to tetracycline. 2 MRSA were isolated from the isolates of S aureus from bovine mastitic milk and revealed multi-drug resistance.

• 대한미생물학회지
• /
• 제5권1호
• /
• pp.27-31
• /
• 1970

Nalidixic acid and six other drugs were studied for in vitro effectiveness against 200 strains of Escherichia coli isolated recently from healthy persons and bactericidal activity of ampicillin against one respective strain of Escherichia coli and Salmonella typhi isolated were also studied. The resutlts obtained by the plate dilution method showed the following percentage of resistance: kanamycin, 2.5%; streptomycin, 12.0%; ampicillin, 13.5%; tetracyclin, 15.5%; chloramphenicol, 17.5%; colistin sulfate, 19.5%. No strains were resistant to nalidixic acid, clearly indicating that nalidixic acid is the most effective drug tested. Ampicillin, measured by test-tube diltion method, was highly bactericidal against Salmonella typhi at the concentration of 2.5mcg/ml and against Escherichia coli at 5mcg/ml.