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Antibiotic Susceptibility of Vibrio parahaemolyticus Isolated from Commercial Marine Products  

Ryu, Seung-Hee (Seoul Metropolitan Government Research Institute of Public Health & Environment, Gangnam Agricultural & Marine Product Inspection Center)
Hwang, Young-Ok (Seoul Metropolitan Government Research Institute of Public Health & Environment, Gangnam Agricultural & Marine Product Inspection Center)
Park, Seog-Gee (Seoul Metropolitan Government Research Institute of Public Health & Environment, Gangnam Agricultural & Marine Product Inspection Center)
Lee, Young-Ki (Department of Public Health, Graduate School, Dankook University)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.4, 2010 , pp. 508-513 More about this Journal
Abstract
Ninety three strains (4.2%) of Vibrio parahaemolyticus were isolated from 2,239 samples of commercial marine products during 2006-2008 in Seoul, Korea. We examined 16 antimicrobial susceptibilities of 93 V. parahaemolyticus isolates. Antibiotic resistance of V. parahaemolyticus was most frequently observed to ampicillin (93.5%), followed by cephalothin (90.3%), streptomycin (87.1%), ticarcillin (55.9%), and amikacin (40.9%). Antibiotic susceptibility was most frequently observed to nalidixic acid, chloramphenicol and ampicillin/sulbactam (100%), followed by trimethoprim/sulfamethoxazole (98.9%), gentamicin and tetracycline (82.8%), and ceftriaxone (63.4%). In addition, the isolates also displayed intermediate resistance to kanamycin (79.6%), ciprofloxacin (64.5%), amikacin (47.3%) and cefoxitin (43.0%). All isolates were resistant to more than two drugs. The most prominent multiple drug resistance was 3 drug resistance (37.6%), followed by 4 drug (24.7%), 5 drug (17.2%), and 6 drug resistance (11.8%). The most prominent multiple drug resistance pattern was the cephalothin-streptomycin-ampicillin resistance pattern (22.6%), followed by cephalothinticarcillin-streptomycin-ampicillin (18.3%) and cephalothin-ticarcillin-streptomycin-ampicillin-amikacin (9.7%). Multiple drug resistance patterns of V. parahaemolyticus from marine products require continuous monitoring.
Keywords
V. parahaemolyticus; antibiotic susceptibility; multiple antibiotic resistance; commercial marine products;
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1 Chakraborty RD, Surendran PK, Joseph TC. Isolation and characterization of Vibrio parahaemolyticus from seafoods along the southwest coast of India. World J. Microbiol. Bio. 24: 2045-2054 (2008)   DOI   ScienceOn
2 Shin HC. Veterinary Drug Residue Monitoring. Korea Food & Drug Administration, Seoul, Korea. p. 2 (2006)
3 FAO/OIE/WHO. Antimicrobial use in aquaculture and antimicrobial resistance. Report of a Joint FAO/OIE/WHO (2006)
4 FDA. Bacteriological Analytical Manual. 8th ed. Method 9. AOAC International, Arinton, VA, USA (1998)
5 Lee YW, Park SG. Laboratory methods of food hygienic microbiology. Shingwang Publishing. Inc., Seoul, Korea (1996)
6 Korea Rural Economic Institute. Food balance sheet. Available from: http://krei.re.kr/kor/statistics_new/food_supply_dtl.php?code=020203&aclass=2&bclass=3. Accessed Dec. 19. 2009
7 Wolfgang W. Medical consequences of antimicrobial use in agriculture. Science 13279: 996-997 (1998)
8 Dixon B. Antimicrobials as growth promoters : Risks and alternatives. ASM News 66: 264-265 (2000)
9 World Health Organization. WHO Global Strategy for Containment of antimicrobial resistance. Available from: http://whqlibdoc.who.int/hq/2001/WHO _CDS_CSR_DRS_2001.2a.pdf. Accessed Dec. 19, 2009.
10 People's solidarity for participatory democracy. Antibiotics Usage monitoring at livestock industry and aquaculture I. Available from: http://blog.peoplepower21.org/StableLife/15717. Accessed Dec. 19, 2009.
11 FAO. Food outlook global market analysis. June 2009. Available from: http://www.fao.org/docrep/011/ai482e/ai482e10.htm. Accessed Dec. 19, 2009.
12 FAO. FAO yearbook. fishery and aquaculture statistics 2006. Available from: http://www.fao.org/fishery/publications/yearbooks/en. Accessed Dec. 19, 2009.
13 ICTSD (International Centre for Trade and Sustainable Development). Fisheries, International Trade and Sustainable Development. Oct. 2006. Available from: http://ictsd.org/i/publications/11852/. Accessed Dec. 19. 2009
14 KFA. Korean Fisheries Yearbook 2008. Korean Fisheries Association, Seoul, Korea (2008)
15 Yeung PSM, Boor KJ. Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaehalyticus. Foodborne Pathog Dis. 1: 74-88 (2004)   DOI   ScienceOn
16 Korea Food & Drug Administration. Food Poisoning Statistics System. Available from: http://e-stat.kfda.go.kr. Accessed Jan. 15, 2010.
17 FAO. Food outlook global market analysis. May 2008. Available from: http://www.fao.org/docrep/010/ai466e/ai466e10.htm#32. Accessed Dec. 19, 2009.
18 Jay JM, Loessner MJ, Golden DA. Modern Food Microbiology. 7th ed. Springer, New York, NY, USA. pp. 549-552 (2005)
19 FAO. The state of world fisheries and aquaculture 2008. Available from: http://www.fao.org/docrep/011/i0250e/i0250e00.HTM. Accessed Dec. 19, 2009.
20 Montville TJ, Matthews KR. Food microbiology an introduction. 2nd ed. ASM Press, Washington DC, USA. pp. 147-152 (2008)
21 Hui YH, Pierson MD, Gorham JR. Foodborne Disease Handbook. 2nd ed. Marcel Dekker Inc., New York, NY, USA. pp. 407-473 (2001)
22 Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scott's Diagnostic Microbiology. 12th ed. Mosby Elsevier, St. Louis, MO, USA. pp. 371-378 (2007)
23 Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology. 3rd ed. Sauders Elsevier, St. Louis, MO, USA. p. 149 (2007)
24 Son KT, Oh EH, Lee TS, Lee HJ, Kim PH, Kim JH. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J. Korean Fish. Soc. 38: 365-367 (2005)   과학기술학회마을
25 Doyle MP. Foodborne bacterial pathogens. Marcel Dekker, Inc., New York, NY, USA. pp. 185-190 (1989)
26 Baker-Austin C, McArthur JV, Tuckfield RC, Najapro M, Lindell AH, Gooch J, Stepanauskas R. Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina USA. J. Food Protect 71: 2552-2558 (2008)
27 Devi R, Surendran PK, Chakraborty RD. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shimp farms along the southwest coast of India. World J. Microbiol. Biot. 25: 2005-2012 (2009)   DOI   ScienceOn
28 Daramola BA, Williams R, Dixon RA. In vitro antibiotic susceptibility of Vibrio parahaemolyticus from environmental sources in northern England. Int. J. Antimicrob. Ag. 34: 499-500 (2009)   DOI   ScienceOn
29 Oh SA. Antimicrobial susceptibility and R-plasmid of Eshcerichia coli isolated from commercial marine products. MS thesis, Dankook University, Cheonan, Chungnam, Korea (2009)
30 Quintoil MN, Porteen K, Beeshmi P. Enteropathogenecity and antibiotic resistance of V. parahaemolyticus isolated form fish samples and fish handlers. Indian Vet. J. 85: 712-715 (2008)
31 Lee JI, Oh YH, Hong CK, Lee YK, Ryu SH, Kim SW, Lee JJ. Epidemic characteristics of Vibrio parahaemolyticus isolated from sea foods in market. Report of Seoul Metropolitan Government Research Institute of Public Health & Environment 33: 32-42 (1997)
32 Chung YH. The Monitoring of Antibiotic Resistant Bacteria from Domestic Fish Farm Environment. Korea Food & Drug Administration, Seoul, Korea. pp. 148-160 (2004)
33 Sen D, De SP, Ghosh SN, Chanda DK, Ghosh A, Pal SC. Antibiotic sensitivity of V. parahaemolyticus from cases of gastroenteritis. Indian J. Med. Res. 65: 628-631 (1977)
34 Park SG, Park SK, Jeong JH, You YA, Lee CH, Kim MS, Kim MH. Molecular characteristics of Vibrio parahaemolyticus isolated from patients in Seoul. Report of Seoul Metropolitan Government Research Institute of Public Health & Environment 38: 31-40 (2002)
35 Feng SW, Huan WW, Chen LH. Contamination of seafood by Vibrio parahaemolyticus in Taiwan. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 20: 140-147 (1987)
36 Lee H, Oh YH, Park SG, Choi SM. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Korean J. Env. Hlth. 33: 16-20 (2007)   과학기술학회마을   DOI
37 Torres Vitela MR, Fernandez Escartin E. Incidence of Vibrio parahaemolyticus in raw fish, oysters, and shrimp. Rev. Latinoam. Microbiol. 35: 267-272 (1993)
38 Lee JI, Oh YH, Kim AK, Lee H, Chung YT, Son YJ, Kim DI, Park SG. Contamination of Escherichia coli and Vibrio parahaemolyticus in marine products. Report of Seoul Metropolitan Government Research Institute of Public Health & Environment, Korea. 39: 300-307 (2003)
39 Son JC, Park SW, Min KJ. Environmental and antimicrobial characteristics of Vibrio spp. isolated form fish, shellfish, seawater and brackfish water samples in Gyongbuk eastern coast. Korean J. Env. Hlth. 29: 94-102 (2003)   과학기술학회마을
40 Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disc susceptibility testing of infrequently isolated or fastidious bacteria. Approved guideline M02-S20. Clinical and Laboratory Standards Institute, Wayne, PA, USA (2010)
41 Jung SC. Establishment of Ccontrol System of Antibiotics for Livestock. Korea Food & Drug Administration, Seoul, Korea. pp. 196-227 (2003)