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http://dx.doi.org/10.5657/kfas.2010.43.6.637

Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater  

Lee, Kuen-Woo (Department of Food Science and Biotechnology, Kunsan National University)
Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.43, no.6, 2010 , pp. 637-641 More about this Journal
Abstract
The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as $1,024-2,048\;{\mu}g{\cdot}mL^{-1}$. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative ${\beta}$-lactamase gene, and the MIC for ampicillin of the mutant strain was $1{\mu}g{\cdot}mL^{-1}$. In conclusion, our findings suggest that the VPA0477 gene acts as a ${\beta}$-lactamase in ampicillin-resistant V. parahaemolyticus strains.
Keywords
Vibrio parahaemolyticus; Ampicillin-resistance; ${\beta}$-lactamase; Deletion mutant;
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Times Cited By KSCI : 6  (Citation Analysis)
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