• Biomolecules & Therapeutics
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• 제13권4호
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• pp.232-239
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• 2005

An aqueous extract of oriental herbal composition named Onchengyeum and curcumin, an antioxidant isolated from turmeric (Curcuma Zonga L.) reduced hepatotoxicity induced by carbon tetrachloride ($CCI_4$). Improved liver function was observed by measuring the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (CRE), total cholesterol (T-CHO), triglyceride (TG), low density lipoprotein cholesterol (LDL-CHO), high density lipoprotein cholesterol (HDL-CHO), total protein (TP), albumin (ALB) and total bilirubin (BIL) in serum. Hepatic parameters monitored were levels of cholesterol (CHO), triglyceride (TG), and malondialdehyde (MDA) and activities of cytochrome P450 (CYP), NADPH-CYP reductase, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx). The histopathological examination showed that the treatment of Onchengyeum and curcumin relieved the ballooning degeneration of hepatocytes which had been generated by $CCI_4$. The results suggested that hepatoprotective effects of Onchengyeum and curcumin possibly are due to their promising antioxidative activity.

• 환경생물
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• 제21권4호
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• pp.410-414
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• 2003

The effect of treatment with pine needle oil complex (complex of pine needle oil and Korean medicinal herbs) upon rat hepatocytes exposed to alcohol was investigated. We compared body weight gain and ratios of liver and kidney to body weight and the serum biochemistry of rats administered both alcohol and Pine needle oil complex to control rats treated with alcohol alone. Pine needle oil complex treatment resulted in a significant reduction in the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and triglycerides (TG) compared to the control rats. These data suggest that Pine needle oil complex represents an excellent candidate for protection of rat hepatocytes from alcohol-mediated damage.

• 한국식품과학회지
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• 제31권4호
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• pp.1096-1100
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• 1999

The protective effect of Bifidobacterium breve K-110, K-111 and B. infantis K-525 on carbon tetrachloride induced hepatotoxicity in rats was investigated. Among them, B. infantis K-525 had the most potent hepatoprotective activity. It reduced serum aspartate aminotransferase and alanine aminotransferase levels to 51% and 80% of the $CCl_{4}-treated$ groups, respectively. In rat liver homogenate intoxificated with $CCl_{4}$, B. infantis K-525 inhibited in vitro as well as in vivo lipid peroxidation more than the other Bifidobacteria.

• 한국식품위생안전성학회지
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• 제11권2호
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• pp.159-164
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• 1996

The antilipidperoxidative and hepatopreventive effects of Aloe water extract (30 mg, 50 mg, 100 mg) were investigated at the levels of liver-total homogenates and the sera of SDrats intoxicated with CCl4 (0.5 cc/100g) and 50% ethanol. We measured MDA (Malondialdehyde) in the liver homogenate, AST (L-Aspartate-2-oxoglutarate aminotransferase) and ALT(L-Alanine-2-oxo-glutraate aminotransferase) in the serum. The analysis of the measurement indicated that Aloe water extract reduced MDA, ALT and AST significantly and their reduction was in relation to dose dependence. In rat liver homogenate intoxicated with ethanol and CCl4, Aloe treatment group markedly inhibited lipidperoxidation by 30%∼70%. In rat serum intoxicated with ethanol and CCl4, Aloe treatment group inhibited AST, ALT by 40%∼90%. In these data Aloe may be used to inhibit or prevent the hapatic toxicity with results from the environmental and alcohlic factors through the further study of its exact antihepatotoxic mechanism.

• 대한의생명과학회지
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• 제8권4호
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• pp.269-273
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• 2002

Dengue fever (DF) is an acute febrile illness caused by dengue viruses in the family Flaviviridae, genus Flavivirus. DF has so far posed any problem in Korea, however it has been recently believed to be associated with oversea's traveler infected with dengue virus. Antibody titers of sera from DF patients against dengue virus were measured by indirect immunofluorescence assay (IFA) and plaque reduction neutralization test (PRNT), including the haematologic test. Three of patients with DF showed highly fluorescent and neutralizing antibody titers by IFA and PRNT assay. Two of them showed higher, remarkably. Meanwhile, one of them was tested and resulted in severe tirombocytopenia, elevated serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities as well as mild leucopenia, increased monocytes and basophils and depressed lymphocytes in haematological differential count.

• Journal of Ginseng Research
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• 제42권3호
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• pp.352-355
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• 2018

In this study, we precisely showed how the Rg3-enriched red ginseng extract (Rg3-RGE) lowers glucose, triglyceride, and low-density lipoprotein (LDL) levels in $LDL^{-/-}$ mice. Aspartate aminotransferase/serum glutamic-oxaloacetic transaminase), alanine aminotransferase /serum glutamate-pyruvate transaminase, and steatohepatitis were found to be reduced, and atheroma formation was inhibited by Rg3-enriched red ginseng extract.

• Biomolecules & Therapeutics
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• 제14권4호
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• pp.207-215
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• 2006

Sachungwhan reduced hepatotoxicity induced by carbon tetrachloride($CCl_4$). Improved liver function was observed by measuring the activities of aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP), blood urea nitrogen(BUN), creatinine(CRE), total cholesterol(TCHO), triglyceride(TG), low density lipoprotein cholesterol(LDL-CHO), high density lipoprotein cholesterol(HDL-CHO), total protein(TP), albumin(ALB) and total bilirubin(BIL) in serum. Hepatic parameters monitored were levels of cholesterol(CHO), triglyceride(TG), malondialdehyde(MDA), content of cytochrome P450(CYP), level of glutathione(GSH), and activities of NADPH-CYP reductase, superoxide dismutase(SOD), catalase(CAT), glutathione S-transferase(GST), glutathione reductase(GR), glutathione peroxidase(GPx). The histopathological examination showed that the treatment of Sachungwhan relieved the ballooning degeneration of hepatocytes which had been generated by $CCl_4$. The results suggested that hepatoprotective effects of Sachungwhan possibly are due to their promising antioxidative activity.

• Journal of Nutrition and Health
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• 제19권4호
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• pp.246-254
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• 1986

이유한 Sprague Dawley 종의 암컷 흰쥐를 두군으로 나누어 각각 pyridoxine이 충분한 식이 (22mg/kg diet)와 pyridoxine이 부족한 식이 (1.2mg/kg diet0로 성장시킨후, 적절한 시기에 임신시켰다. 태어난 새끼가 이유할 때 결핍식이군을 둘로 나누어, 한쪽은 pyridoxine이 충분한 식이로 바꾸어준후, 세군의 쥐를 생후 10-주까지 성장시켰다. 각 군의 새끼쥐가 각각 0, 3, 7, 10 주 되었을대, 간의 mitochondria 및 cytosolic fraction 에 있는 Asparate aminotransferase [ EC 2.6.1.1] activity 및 pyridoxal phosphate의 함량을 측정하였다. 결핍군의 간 aspartate aminotransterase activity는 mitochondria 및 cytosolic fraction에서 각각 대조군에 비해 유의적으로 낮았으며 10-4 M pyridoxal phosphate를 첨가한 후 측정한 total enzyme activity 는 거의 대조군과 비슷하였다. 3 주이후 pyridoxine 이 충분한 식이로 바구어준 회복군에서도 대조군에 비해 유의적으로 낮았으나, 결핍군보다는 높았다. 이두 fraction 에서의 pyridoxal phosphate 함량은 결핍군이 대조군보다 현저히 낮았으며, 회복군은 대조군과 비슷하였다. 이로써, 장기간에 걸친 pyridoxine결핍이간의 aspartate aminotransferase activity 및 pyridoxal phosphate에 현저한 영향을 미치며 이유기 이후의 식이보충에 의해서도 enzyme activity는 쉽게 회복되지 않음을 알 수있다.

• 농약과학회지
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• 제9권4호
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• pp.338-346
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• 2005

Carbendazim의 랫드 간 해독체계에 대한 영향을 검색하기 위하여 간장독성의 지표 효소인 혈장 ALT(alanine aminotransferase) 및 AST(aspartate aminotransferase) 활성, 간에 대한 독성, 해독 및 대사에 대한 영향을 구명하기 위하여 간 GSH(glutathione), GST(glutathione-S-transferase), cytochrome P450 및 cytochrome P450 reductase 활성을 375, 750, 1,500 mg/kg 약량에서 측정한 결과 혈장 ALT 및 AST 활성이 120분 후에 약간의 증가가 있었으나 특이한 독성증상은 관찰되지 않았다. GSH는 고농도와 중농도 120분에서 20%의 함량증가가 있었고, GST는 120분까지 $36{\sim}50%$ 정도의 활성저해가 있었으나 240분에는 활성이 회복되었다. Cytochrome P450함량은 60분까지 $25{\sim}50%$까지 함량이 저하되었으나 120분에 70%이상 회복되었고, 240분 저농도에서는 거의 회복되었으며, cytochrome P450 reductase도 120분까지 $25{\sim}50%$ 활성저해가 있었으나, 240분에는 무처리 군의 활성과 유사하게 회복되어 대사관련 효소에 대한 손상은 크지 않을 것으로 판단되었다. 이상의 결과에서 benzimidazole 계 살균제인 carbendazim은 생체내 해독 및 대사관련 체계에 영향이 적은 농약으로 급성적인 중독은 일으키지 않을 것으로 판단되었다.

• 한국유기농업학회지
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• 제22권3호
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• pp.423-434
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• 2014

영지버섯으로 배양된 유기 한국 토종 쌀이 항당뇨 효과에 미치는 영향을 알아보기 위하여 영지버섯의 액체배양과 유기 한국 토종 쌀에서의 영지버섯 배양 및 생산된 유기 한국 토종 영지버섯 쌀을 당뇨가 유발된 흰쥐에 경구투여한 후, 혈당, 혈청 지질 및 alanine aminotransferase(ALT)와 aspartate aminotransferase(AST) 활성을 조사하였다. 영지버섯 액체배양 조건은 pH가 4.5와 5.0이고, 온도는 $30^{\circ}C$에서 최적인 것으로 나타났고, 유기 한국 토종 영지버섯 쌀 생산 조건으로는, 물리적 형태나 배양 조건 등을 고려했을 때, 1kg의 토종 쌀에 7.5%의 종균을 접종하여, $28^{\circ}C$에서 9일간 배양하는 것으로 나타났다. 혈당 강하 효과 시험에서 당뇨 유발군에서, 영지버섯으로 배양된 유기 한국 토종 쌀의 급여군에 있어서의 혈당의 감소정도가 대조군보다 유의하게 감소하였다. 또한 영지버섯으로 배양된 유기 한국 토종 쌀의 급여군에 있어서의 혈장 total cholesterol과 triglyceride는 대조군에서 보다 낮은 수치를 나타내었고, ALT와 AST 활성은 대조군에서 보다 낮은 수치를 나타내었다.