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Protective Effect of Sachungwhan against CCl4-induced Hepatotoxicity  

Koo, Ja-Young (Duksung Women's University)
Jung, Ki-Hwa (Duksung Women's University)
Publication Information
Biomolecules & Therapeutics / v.14, no.4, 2006 , pp. 207-215 More about this Journal
Abstract
Sachungwhan reduced hepatotoxicity induced by carbon tetrachloride($CCl_4$). Improved liver function was observed by measuring the activities of aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP), blood urea nitrogen(BUN), creatinine(CRE), total cholesterol(TCHO), triglyceride(TG), low density lipoprotein cholesterol(LDL-CHO), high density lipoprotein cholesterol(HDL-CHO), total protein(TP), albumin(ALB) and total bilirubin(BIL) in serum. Hepatic parameters monitored were levels of cholesterol(CHO), triglyceride(TG), malondialdehyde(MDA), content of cytochrome P450(CYP), level of glutathione(GSH), and activities of NADPH-CYP reductase, superoxide dismutase(SOD), catalase(CAT), glutathione S-transferase(GST), glutathione reductase(GR), glutathione peroxidase(GPx). The histopathological examination showed that the treatment of Sachungwhan relieved the ballooning degeneration of hepatocytes which had been generated by $CCl_4$. The results suggested that hepatoprotective effects of Sachungwhan possibly are due to their promising antioxidative activity.
Keywords
Sachungwhan; $CCl_4$; free radical; lipid peroxidation; hepatotoxicity;
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1 Antebi, H., Ribiere, C., Sinaceur, J., Abu-Murad, C., Nordmann, R. (1984). Involvement of oxygen radicals in ethanol oxidation and in the ethanol-induced decrease in liver glutathion. in : Bors, W., Saranm, M., Tait, D., eds. Oxygen Tadicals in Chemistry and Biology. New york., p.757-760
2 Carlberg, I., Mannervik, B. (1985). Glutathione reductase. Methods Enzymol. 113, 484-490   DOI
3 Comporti, M. (1993). Lipid peroxidation : An overview. In : Free Radicals: From Basic Science to Medicine(Molecular and cell biology updates), Poli, G., Albano, E., Dianzani, M.U. eds. Birkhause Verlag, Basel. Switzerland. p.65-79
4 David, R.M., Nerland, D.E. (1983). Induction of mouse liver glutathione S-transferase by ethanol. Biochem. Pharmacol 32, 2809-2811   DOI   ScienceOn
5 Ellman, G.L, (1959). Tissue sulfhydryl groups. Arch Biochem. Biophys. 82, 70-77   DOI   ScienceOn
6 Freidovich, I. (1999). Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann. NY. Acad. Sci. 893, 13-18   DOI
7 Fridovich, I. (1995). Xanthine oxidase, CRC handbook of methods for oxygen radical research. CRC Press. New york
8 Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione Stransferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139
9 Hagymasi, K., Kosis, I., Lugasi, A., Fesher, J., Blazovics, A., 2002. Extrahepatic biliary obstruction:can silymarin protect liver function- Phytother. Res. 16(Suppl. 1), s78-s80
10 McCay, P.B., Lai, E.K., Poyer, J. L. (1984). Oxygen-and carboncentered free radical formation during carbon tetrachloride metabolism. J. Biochem. 254, 2135-2143
11 McCord, J.M. (2000). The evolution of free radicals and oxidative stress. Am. J. Med. 108, 652-659   DOI   ScienceOn
12 Nong, J.H. (1986). Reprinting with additions of manbunghoychun. Jungkukkukwonsukongsa. last volume p.83
13 Omura, T., Sato, R. (1964). The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature., II. Solubilization, purification, and properties. J. Biol. Chem. 239, 2370-2378
14 Recknagel, R.O. (1967). Carbon tetrachloride hepatotoxicity. Pharmacol Rev. 19, 145-208
15 Oruc, E.O., Uner, N. (2000) Combined effects of 2,4-D and azinphosmethyl on antioxidant enzymes and lipid peroxidation in liver of Oreochromis niloticus. Comp Biochem Physiol C Toxicol Pharmacol. 127, 291-296
16 Park, S.S., Yeom, T.H., (1984). lecture of today's chinese(herb) medicine. Seoul Hanglim publishing company. p.185, 186, 204, 507, 518
17 Poli, G., Albano, E., Dianzani, M.U. (1987). The role of lipid peroxidation in liver damage. Chem. Phys. Lipids. 45, 117-142   DOI   ScienceOn
18 Aebi, H. (1974). Catalase Methods of Enzymatic analysis. 3rd ed, Verlag. Chemie. 2, 673-684
19 Ahmed, R.S., Seth, V., Banerjee, B.D. (2000). Influence of dietary ginger (Zingiber officinales Rose) on antioxidant defense system in rat: comparison with ascorbic acid. Indian J Exp Biol 38, 604-606
20 Uchiyama, M., Mihara, M. (1978). Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analytical Biochemistry. 86, 271-278   DOI   ScienceOn
21 Y. Sun. L. W. Oberley. (1996). Redox regulation of transcriptional activators. Free Rad. Biol. Med. 21, 335-348   DOI   ScienceOn
22 Tappel, A.L. (1978). Glutathione peroxidase and hydroperoxides. Methods Enzymol. 52, 506-513   DOI
23 Niki, E. (1993). Antioxidant defenses in eukariotic cells: An Overview. In: Free radicals: From Basic Science to Medicine(Molecular and cell biology update), Poli, G., Albano, E., Dianzani, M.U. eds. Birkhauser Verlag, Vasel. Switzerland., p.365-368
24 Kaynar, H., Meral, M., Turhan, H., Keles, M., Celik, G., Akcay, F. (2005). Glutathione peroxidase, glutathione-S-transferase, catalase, xanthine oxidase, Cu-Zn superoxide dismutase activities, total glutathione, nitric oxide, and malondialdehyde levels in erythrocytes of patients with small cell and non-small cell lung cancer. Cancer Lett. 227, 133-139   DOI   ScienceOn
25 Ray, A., Banerjee, B.D. (1998). Stress, free radicals and the immune response: modulation by drugs. Arch. Pharmacol. 358 (Suppl. 2), 739-744
26 Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275
27 Ho, Y.S., Crapo, J.D. (1988). Isolation and characterization of complementary DNAs encoding human manganese-containing superoxide dismutase. FEBS Lett. 229, 256-260   DOI   ScienceOn
28 Koner, B.C., Banerjee, B.D., Ray, A. (1998). Organochlorine pesticide-induced oxidative stress and immune suppression in rats. Indian J Exp Biol. 36, 395-398
29 Koo, B.H. (1997). Lexicologic Doneuibogam. Korean dictionary research p.187, 192
30 Kwon, Y.M., Park, S.D.(2002). Effects of Sachungwhan and itscomponent groups on Antioxidant Activities. Kor. J. Herbology, 2, 225-233
31 Svingen, B.A., Buege, J.A., O'Neal, F.O., Aust, S.D. (1979). The mechanism of NADPH-dependent lipid peroxidation. The propagation of lipid peroxidation. J Biol. Chem. 254, 5892-5899
32 Recknagel, R.O, Glende, Jr, E.A., Dolak, J.A., Waller, R.L. (1989). Mechanisms of carbon tetrachloride toxicity. Pharmacol. Ther. 43, 139-154   DOI   ScienceOn
33 Sies, H., Akerboom, T.P. (1984). Glutathione disulfide (GSSG) efflux from cells and tissues. Methods Enzymol 105, 445-451   DOI
34 Strobel, H.W., Digman, J.D. (1978). Biological oxidations, microsomal, cytochrome P-450, and other hemoprotein systems, Methods in enzymology : biomembrames, II. Academic press. New york p.89-96
35 James, J.L., Moody, D.E., Chan, C.H., Smuckler, E.A. (1982). The phospholipids of the hepatic endoplasmic reticulum. Structural change in liver injury. Biochem. J. 206, 203-210   DOI
36 Song, H.J. (1984). The effect of Hwangryonhaedoktang on the immune response to sheep red blood cells. Wonkwang Univ. Oriental Med. J. 2, 195-206