• Title/Summary/Keyword: Amino acid content

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Chemical Composition and Bioactivity of Korean Green Tea (Camellia sinensis) Pollen collected by Honey Bee (한국 녹차 화분의 화학적 조성과 기능성 연구)

  • Jang, Jae-Seon
    • The Korean Journal of Food And Nutrition
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    • v.31 no.1
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    • pp.89-93
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    • 2018
  • In this study, we investigated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of green tea (Camellia sinensis) pollen grains collected by Apis mellifera bees, for use as a health food. The crude protein and fat content was estimated at 26.14% and 3.49%, respectively. Eighteen amino acids were identified in green tea pollen, including 8 essential amino acids and 10 non-essential amino acids. The predominant amino acids were glutamic acid, proline and aspartic acid accounting for about 33.3% of total free amino acids. The concentration of vitamin C was the highest value of 35.7%, followed by $B_3$ and $B_2$ among the detected vitamins. The predominant minerals were potassium (790.32 mg/100g), followed by phosphorus (707.52 mg/100g) and sulfur (302.67 mg/100g), whereas copper, zinc and sodium were detected as minor elements. The antioxidant activity and phenolic content accounted for 33.8% at $500{\mu}g/mL$ extract and $2.55 {\mu}g/mg$, respectively.

Effect of Early Soybean Seeding on Isoflavone Content and Amino Acid Composition (콩 조기파종이 Isoflavone 함량 및 아미노산 조성에 미치는 영향)

  • Jong Hyuk Kim;Mi Ha Jang;Ju Hee Nam;Il Rae Rho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.67 no.4
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    • pp.242-252
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    • 2022
  • Soybeans have various applications, and are a major source of proteins, carbohydrates, and fats. As climate warming has accelerated in recent years, this study was conducted to analyze the effect of different sowing periods on component changes in protein, amino acid, and antioxidant contents of various soybean varieties. The isoflavone content of soybeans that were sown early did not differ with the sowing period. Based on the results, only the total isoflavone content was confirmed in functional soybean cultivars such as Cheongja-5, which had blue-colored seeds; Pungsan and Sowon, which used soybean sprouts; and Jinyang, which lacked a fishy smell. There was no significant difference in the changes in protein and amino acid content in soybean cultivars with respect to the sowing time. The protein and amino acid contents in early-sown soybeans tended to be lower than that in timely sown soybeans. In most varieties, the content of proline was the highest in early-sown soybeans, confirming the presence of stress due to early sowing.

Changes in free amino acid content and hardness of beef while dry-aging with Mucor flavus

  • Hanagasaki, Takashi;Asato, Naokazu
    • Journal of Animal Science and Technology
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    • v.60 no.7
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    • pp.19.1-19.10
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    • 2018
  • Background: A mold strain thought to be suitable for dry-aging process was isolated. The information about the scientific aspects of molds related to dry-aging beef is scarce. We, therefore, conducted aging trials to determine the characteristics of the isolated mold strain associated with dry-aging process. Specifically, during the dry-aging of beef with the mold strain, the changes in the free amino acid content, hardness, productive loss, drip and cooking loss were analyzed. These characteristics were compared with those obtained while dry-aging in the absence of a mold. Results: The isolated mold strain was identified as Mucor flavus. The free amino acid content in the mold-aging beef decreased or remained constant during the aging process. However, that in the trimming sections of the beef dramatically increased in the presence of mold. In addition, hardness of mold-aging beef gradually decreased during the aging process and finally decreased significantly. Conclusion: Amino acids such as GABA (gamma-aminobutyric acid), proline, and aspartic acid were produced by our mold strain, M. flavus during its growth on beef meat, and the mold conferred savory odors to the dry-aged beef.

Comparison of amino acid and free amino acid contents between cap and stipe in Flammulina velutipes and Pleurotus ostreatus (팽이와 느타리버섯 자실체의 갓과 대에서 아미노산 및 유리아미노산 함량 비교)

  • Kim, Jung-Tae;Kim, Mi-Jung;Jhune, Chang-Sung;Shin, Pyung-Gyun;Oh, YounLee;Yoo, Young-Bok;Suh, Jang-Sun;Kong, Won-Sik
    • Journal of Mushroom
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    • v.12 no.4
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    • pp.341-349
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    • 2014
  • The composition and content of amino acid and free amino acid were separately compared in cap and stipe of fruitbody obtained from different color strains in winter mushroom and oyster mushroom strains. The result showed different kinds and amount of amino acids according to the mushroom parts, strains and kinds. Tryptophane was not detected in any mushrooms. Alanine was also not detected in all oyster mushroom strains. The content of glutamic acid was the highest and that of methionine, phenylalanine and cysteine were very low in all mushroom strains. When compared by parts, the ratio of amono acids showed similar tendency in composition. Generally the content of amono acids showed higher in caps than in stipes. The profile and content of free amino acid showed a lot of variation between cap and strip in the intra and inter mushrooms.

AMINO ACID DIGESTIBILITY AS AFFECTED BY VARIOUS FIBER SOURCES AND LEVELS 2. THE RELATIONSHIP BETWEEN FIBER LEVELS AND AMINO ACIDS DIGESTIBILITY

  • Nongyao, A.;Han, In K.;Choi, Yun J.;Lee, N.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.4
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    • pp.353-361
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    • 1990
  • A number of correlation and regression analyses were performed on data from apparent and true digestibility of amino acids at ileal and fecal level with finishing pigs, in order to investigate whether these amino acid digestibilities could be predicted with certainly degree on their fiber fractions content (chemical analysis). The data comprised 16 diets varying in 4 levels of crude fiber and from 4 fiber sources. The relationships between fiber fractions including crude fiber (CF), NDF, ADF lignin and cellulose contents on apparent and true digestibility of almost all amino acids in both ileal and fecal level were negative, except glutamine at fecal level. In apparent digestibility at ileal level, the correlations of fiber fractions were moderate (r of NDF = 0.53 to 0.63; ADF, 0.50 to 0.77; cellulose, 0.50 to 0.75), with an exception of CF content was relatively high (r of 0.58 to 0.81). The correlations to true digestibility of amino acids were weaker. In case of at fecal level, the higher correlation (negative) was found with NDF than CF content. Estimations of amino acids digestibility were performed using regression equation. The data showed that apparent digestibility of amino acids could be estimated for almost amino acids except arginine, threonine, valine and tyrosine at fecal level and phenylalaine, valine and glycine at ileal level. The best prediction at ileal and fecal level ($r^2=0.55-0.77$ and 0.52-0.76), respectively was obtained with NDF content. Prediction for true digestibility of amino acids, none of fiber fractions could be estimated for arginine, leucine and valine at all collection levels. At ileal level, CF could be used for most of amino acids except phenylalanine, glycine and praline; cellulose, only for lysine and methionine and NDF, only for proline. At fecal level, glutamine digestibility could be estimated only from CF and ADF content. The best predictor at ileal level was ADF content whereas at fecal level was NDF content. These results indicate that lignin content could not be used as predictor for all amino acids at both levels neither in apparent nor true digestibility.

Comparative Studies on the Composition of Korean Human and Cow's Milk (I) -Amino Acid Composition of Human and Cow's Milk of Korea- (한국인(韓國人) 모유(母乳)와 우유(牛乳)의 성분(成分) 조성(組成)에 관(關)한 비교(比較) 연구(硏究) (I) -한국인(韓國人) 모유(母乳)와 우유중(牛乳中)의 Amino산(酸) 조성(組成)에 관(關)한 연구(硏究)-)

  • Ko, Young-Su;Kim, Jung-Ja;Han, In-Ja
    • Journal of Nutrition and Health
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    • v.3 no.2
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    • pp.87-94
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    • 1970
  • The amino acid composition of human and cow's milk represents a standard of reference in infantnutrition. The amino acid compositions determined by automatic amino acid analyzer, Yanagimote Model LC-5. Protein in the human and cow's milk were found to be hydrolyzed to yield free amino acids. Qualitative data for free amino acids in the milk are as follows: 1) Amounts of acidic amino acids such as glutamic and aspartic acid in cow's milk were obserbed to be about 2 times compared with human milk and it is considered that the abundance in these amino acids may contribute significantly to the specific flavor of cow's milk. 2) It is much interesting that in the human milk the contents of sulfur-containing amino acids were high comparatively better than cow's milk; cystine was found to be 3 times ana methionine, 2 times. 3) In the human milk a high content of some essential amino acids such as threonine, isoleucine and leucine was demonstrated and a specific flavor sweet amino acids. 4) Large amounts of basic amino acid such as histidine was found to occur in human milk and arginine in cow's milk.

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Studies on the Ripening of Beef at Adding the Proteolytic Enzyme I. Changes of Free Amino Acid in Beef According to the Papain Addition (단백질(蛋白質) 분해효소(分解酵素) 첨가시(添加時) 우육(牛肉)의 숙성(熟成)에 관(關)한 연구(硏究) 제(第)1보(報) Papain처리(處理)에 의한 우육(牛肉)의 유리(遊離) Amino Acid변화(變化)에 관(關)하여)

  • Youn, J.E.;Oh, S.H.;Hwang, C.S.
    • Korean Journal of Food Science and Technology
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    • v.5 no.2
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    • pp.71-77
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    • 1973
  • The results, which was analytically surveyed the free amino acids by the automatic amino acid analyzer adding the enzyme on the Korean cow's fore shank muscles, are as follows: 1. The content of free amino acids in the fore shank muscles, without addition of the enzyme orderly contains alanine, glutamic acid, lysine, glycine, histidine, leucine, threonine, arginine, cystine, serine, proline, isoleucine, phenylalanine, tyrosine, methionine, aspartic acid and valine. 2. In accordance with the addition of the enzyme, by 0.01%, 0.05% and 0.1% the nine free amino acids of glutamic acid, glycine, alanine, cystine, valine, isoleucine, leucine, lysine and arginine were continuosly increased. 3. Proline and histidine were decreased at the enzyme addition of 0.01% after showing the high content at the control, but the quantity of free amino acids was increased according to the increase of the quantity of the enzyme. 4. Aspartic acid, threonine, serine, methionine, tyrosine and phenylalanine were increased till the enzyme addition of 0.05% and remarkably decreased from 0.1%. 5. At cooking the meat, the quantity of the enzyme addition was most effective at 0.05% of meat weight.

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Analysis of the Amino Acids Content of Three Neopyropia Dentata Cultivars under the Two Different Aquafarm Environment in Haenam, Korea (해남의 김 양식장별 잇바디돌김(Neopyropia dentata) 3품종의 아미노산 성분 분석)

  • Hye Ri Nam;Sung Je Choi
    • Journal of Practical Agriculture & Fisheries Research
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    • v.25 no.3
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    • pp.5-13
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    • 2023
  • This study aims to assess the water quality of the Eoran and the Imha aquafarm with different aquafarm environments in Haenamn-gun, and analyzed the composition of total amino acids (TAAs) and free amino acids (FAAs) in three Neopyropia dentata cultivars (Yuldo, Supum1 and 2) in two aquafarms. Mean water temperature ranged from 22.9 to 10.9℃ during September to November 2018. In Eoran aquafarm, the water quality analysis showed that NO2-N was high in September, NH4-N and COD in October, and NO3-N, DIN, and DIP in November. In Imha aquafarm, the water quality analysis showed that NH4-N and COD was high in September, NO3-N and DIN in October, and NO2-N and DIP in November. We confirmed the proximate composition, amino acid composition (TAA/FAA) in two auqufarms. In the Eoran aquafarm, the 'Yuldo' cultivar had significantly higher crude lipid content than two other cultivars (Supum 1 and 2). The 'Supum1' cultivar had significantly higher moisture content, whereas the highest content of crude protein, crude lipid, crude ash, and carbohydrates was found in the 'Supum2' cultivar. In the Imha aquafarm, the content of crude lipid and crude ash was highest in the 'Yuldo' cultivar. The highest content of crude protein and carbohydrates was found in the 'Supum1' cultivar, while the 'Supum2' cultivar had the highest content of moisture. The highest concentration of glutamic acid belong to TAAs is observed in all cultivars from Eoran and Imha aquafarm, while all cultivars in two aquafarm also contained higher content of alanine among the detected FAAs.

Chemical Composition of Lutus Seed(Nelumbo nucifera Gaertner) and Their Lipid and Protein Composition (연밥의 유지와 단백질의 구성에 관한 연구)

  • Shin, Dong-Hwa;Kim, In-Won;Kwon, Kyoung-Soohn;Kim, Myoung-Sook;Kim, Mi-Ra;Choi, Ung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.6
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    • pp.1187-1190
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    • 1999
  • Lotus seed(Nelumbo nucifera Gaertner), known as traditional medicine as an antifebrile, antipsychotic, and cantihypertensive agent, was analyzed the chemical composition of lipid and protein. The seed com posed of 12.2% moisture, 2.3% crude lipid, 19.5% crude protein, 61.3% carbohydrate, 2.1% crude fibre, and 4.1% ash. The lipid showed iodine value of 97.9 that is lower than that of soybean oil and sesame oil, and similar to peanut oil and cotton seed oil. The fatty acid composition of the oil were the highest in content of linoleic acid which occupied 58.3% and saturated vs unsaturated fatty acid was 20.9:79.1. Especially behenic acid content, 6.9%, was higher than other plant oils. Sixteen amino acids were detected in the protein from the seed and glutamic acid content was the highest as 4.5% in dehulled kernel. The portion of essential amino acid was 31.1%.

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Comparative Analysis of the Amino Acids and Effective Components from Viola mandshurica (자화지정(Viola mandshurica)의 아미노산과 유용성분 비교 분석)

  • Lee, Yang-Suk;Kim, Nam-Woo
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.699-705
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    • 2008
  • This study compared amino acids and effective components of Viola mandshurica produced in Korea with those of Chinese plants, as part of a study on nutritional andfunctional materials, to aid in the development of a valuable food. The contents of reducing sugar, free sugar, polyphenols, and soluble protein in the flowers of V. mandshurica were higher than in other fractions, being 10,485.11 mg%, 118.85 mg%, 1,259.26 mg%, and 23.67 mg%, respectively. Total flavonoid content of Chinese leaves was the highest at 2,370.75 mg%, followed by 1,844.43 mg% in Korean leaves. Free amino acids were highest in Korean leaves (5,345.21 mg%), followed by roots (4,692.24 mg%), flowers (3,807.87 mg%), and leaves of China plants (3,613.81 mg%). Arginine accounted for 55% of free amino acids in the roots, and all plant fractions contained more valine, aspartic acid, glutamic acid, alanine, and arginine, than other amino acids. The total content of amino acid derivatives was the highest (9,058.80 mg%) in Korean leaves. Especially, $\alpha$-aminoadipic acid constituted over 50% of total amino acid derivatives in all plant parts of V. mandshurica. When mineral contents were analyzed, leaves grown in China showed the highest content of 5,917.57 mg%, and the contents of K, Ca, and Mg were much greater than those of other minerals, together comprising over 95% of total mineral content in leaves and flowers from Korea.