• 한국컴퓨터정보학회논문지
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• 제7권4호
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• pp.89-93
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• 2002

본 논문에서는 32비트 DSP에 사용 가능한 ALU를 설계하였다. 이 ALU는 32비트 연산을 기본 단위로 하고 있으며 5단 파이프라인 중에서 execution 단계에 해당된다. ALU에서 지원하는 기능은 덧셈, 뺄셈, 나눗셈과 같은 산술연산, AND, XOR과 같은 논리연산, 그리고 쉬프트 등이다. 기능별로 여러 기능 블록을 사용하지 않는 대신 몇 개의 기능 블록만을 만들고, 회로 동작이 이 기능 블록들을 공유하도록 설계하였으며, ALU를 설계하기 위해 각 기능 블록을 HDL로 기술하여 시뮬레이션을 수행하였다. 이ALU는 32 비트 DSP에 사용 가능하도록 설계되었다.

• 생명과학회지
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• 제14권6호
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• pp.1028-1039
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• 2004

6500만년동안, Alu 서열은 RNA-중합효소 III에 의한 전사체를 통해 증폭해왔고, 영장류 게놈 내에 약 140만 복사의 수에 도달되었다. 그들은 가동성 인자 중에서 가장 큰 집단이며, 인간 게놈의 $10\%$를 구성한다. Alu 서열이 유전적으로 기능이 없다고 생각되었지만, 최근 많은 연구자들이 새로운 기능 및 질병과의 관련성을 증명해왔다 이들 Alu 서열은 삽입돌연변이, Alu-매개 재조합, 유전자 발현에 대해 유전자 전환 그리고 스플라이싱 사이트를 유발하고, 유전자 구조, 단백질 서열, 스플라이싱 모티프와 발현 양상에 영향을 준다. 우리는 Alu의 구조와 기원, 그들 패밀리의 컨센서스 서열, Alu의 진화와 분포 그리고 그들의 기능에 대하여 요약 정리하였다. 또한 영장류의 진화과정에 있어 질병과 관련하여 Alu 패밀리의 새로운 연구방향을 제시하였다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 추계학술대회
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• pp.540-543
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• 2013

본 논문은 SIMT 구조 기반의 멀티코어 GPGPU의 통합 ALU를 설계하였다. 조건부 분기 명령어, 데이터 이동 명령어, 정수형 산술 연산 명령어, 부동소수점 산술 연산 명령어를 처리할 수 있으며 멀티코어 GPGPU의 다양한 형태의 병렬처리 기능을 지원하기 위하여 다 수의 ALU가 탑재된다. 각 명령어 연산의 처리방식의 공통성을 회로 수준에서 통합하여 최소의 크기로 ALU를 설계하는 것이 본 논문의 주안점이다. 모든 명령어는 테스트 프로그램을 작성하여 실험하였고 CPU로 연산한 결과와 비교하여 본 논문의 ALU가 정상적으로 동작함을 검증하였다. 본 논문에서 설계한 통합 ALU의 크기는 약 2만 게이트이며 최대 동작주파수는 430MHz이다.

• Genomics & Informatics
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• 제4권1호
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• pp.11-15
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• 2006

The aim of this study is to identify hRad21-binding sites in human chromosome, the core component of cohesin complex that held sister chromatids together. After chromatin immunoprecipitation with an hRad21 antibody, it was cloned the recovered DNA and sequenced 30 independent clones. Among them, 20 clones (67%) contained repetitive elements including short interspersed transposable elements (SINE or Alu elements), long terminal repeat (LTR) and long interspersed transposable elements (LINE), fourteen of these twenty (70%) repeats clones had Alu elements, which could be categorized as the old and the young Alu Subfamily, eleven of the fourteen (73%) Alu elements belonged to the old Alu Subfamily, and only three Alu elements were categorized as young Alu subfamily. There is no CpG island within these selected clones. Association of hRad21 with Alu was confirmed by chromatin immunoprecipitation-PCR using conserved Alu primers. The primers were designed in the flanking region of Alu, and the specific Alu element was shown in the selected clone. From these experiments, it was demonstrated that hRad21 could bind to SINE, LTRs, and LINE as well as Alu.

• Genomics & Informatics
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• 제14권3호
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• pp.70-77
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• 2016

Transposable elements are one of major sources to cause genomic instability through various mechanisms including de novo insertion, insertion-mediated genomic deletion, and recombination-associated genomic deletion. Among them is Alu element which is the most abundant element, composing ~10% of the human genome. The element emerged in the primate genome 65 million years ago and has since propagated successfully in the human and non-human primate genomes. Alu element is a non-autonomous retrotransposon and therefore retrotransposed using L1-enzyme machinery. The 'master gene' model has been generally accepted to explain Alu element amplification in primate genomes. According to the model, different subfamilies of Alu elements are created by mutations on the master gene and most Alu elements are amplified from the hyperactive master genes. Alu element is frequently involved in genomic rearrangements in the human genome due to its abundance and sequence identity between them. The genomic rearrangements caused by Alu elements could lead to genetic disorders such as hereditary disease, blood disorder, and neurological disorder. In fact, Alu elements are associated with approximately 0.1% of human genetic disorders. The first part of this review discusses mechanisms of Alu amplification and diversity among different Alu subfamilies. The second part discusses the particular role of Alu elements in generating genomic rearrangements as well as human genetic disorders.

• BMB Reports
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• 제52권3호
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• pp.196-201
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• 2019

The Alu element, the most abundant transposable element, is transcribed to Alu RNA. We hypothesized that the PIWI protein regulates the expression of Alu RNA in retinal pigment epithelial (RPE) cells, where accumulated Alu RNA leads to macular degeneration. Alu transcription was induced in RPE cells treated with $H_2O_2$. At an early stage of oxidative stress, PIWIL4 was translocated into the nucleus; however, subsequently it was sequestered into cytoplasmic stress granules, resulting in the accumulation of Alu RNA. An elevated amount of Alu RNA was positively correlated with the disruption of the epithelial features of RPE via induction of mesenchymal transition. Therefore, we suggest that oxidative stress causes Alu RNA accumulation via PIWIL4 sequestration into the cytoplasmic stress granules.

• 공학논문집
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• 제2권1호
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• pp.31-37
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• 1997

본 논문에서의 ALU는 덧셈, 뺄셈, 1증가, 1 감소, 2의 보수 등의 산술 연산을 수행하는 산술 연산 회로, 논리합, 논리곱, 배타논리합, 부정과 같은 논리 연산을 수행하는 논리 연산 회로, 쉬프트 연산 및 산술 혹은 논리 연산 회로의 연산 결과를 데이터 버스로 전송하는 기능을 담당하는 쉬프터로 구성되며, 이러한 기본적인 ALU 기능과 관련된 명령어는 Z80 명령어에서 추출하여 ALU의 내부 회로를 설계하였고, 이 설계된 회로를 그래픽 화면으로 구성하여 데이터의 연산이 ALU 내부에서 어떤 과정과 경로를 거쳐 수행되는 가를 비트 및 논리 게이트 단위까지 처리하여 ALU 구조와 단계별 연산 과정을 그래픽 형태로 학습하는 교육 시스템이다.

• Genomics & Informatics
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• 제2권2호
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• pp.86-91
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• 2004

Even though it represents $6-13\%$ of human genomic DNA, Alu sequences are rarely found in coding regions. When in exon region, over $80\%$ of them are found in 3' untranslated region (UTR). Pseudogenes are an important component of human genome. Their functions are not clearly known and the mechanism of how they are generated is still debatable. Both the Alu and Pseudogenes are important research problems in molecular biology. mRNA is thought to be a prime source of pseudogene and active research is going on its molecular mechanism. We report, for the first time, that mRNAs containing Alu repeats at 3' UTR has a significantly high correlation with processed pseudogenes, suggesting a possibility that Alu containing mRNAs have a high tendency to become processed pseudogenes. It is known that about $10\%$ of all human genes have been transposed. Transposed genes at 3' UTR without Alu repeat have about two processed pseudogenes per gene on average while we found with statistical significance that a transposed gene with Alu had over three processed Pseudogenes on average. Therefore, we propose Alu repeats as a new and important factor in the generation of pseudogenes.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9797-9800
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• 2014

Background: Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and Southeast Asia. Alu elements are among the most prevalent repetitive sequences and constitute 11% of the human genome. Although Alu methylation has been evaluated in many types of cancer, few studies have examined the levels of this modification in serum from NPC patients. Objective: To compare the Alu methylation levels and patterns between serum from NPC patients and normal controls. Materials and Methods: Sera from 50 NPC patients and 140 controls were examined. Quantitative combined bisulfite restriction analysis-Alu (qCOBRA-Alu) was applied to measure Alu methylation levels and characterize Alu methylation patterns. Amplified products were classified into four patterns according to the methylation status of 2 CpG sites: hypermethylated (methylation at both loci), partially methylated (methylation of either of the two loci), and hypomethylated (unmethylated at both loci). Results: A comparison of normal control sera with NPC sera revealed that the latter presented a significantly lower methylation level (p=0.0002) and a significantly higher percentage of hypomethylated loci (p=0.0002). The sensitivity of the higher percentage of Alu hypomethyted loci for distinguishing NPC patients from normal controls was 96%. Conclusions: Alu elements in the circulating DNA of NPC patients are hypomethylated. Moreover, Alu hypomethylated loci may represent a potential biomarker for NPC screening.

• 한국초전도ㆍ저온공학회논문지
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• 제7권1호
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• pp.9-12
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• 2005

We have constructed an RSFQ 4-bit Arithmetic Logic Unit (ALU) in a pipelined structure. An ALU is a core element of a computer processor that performs arithmetic and logic operation on the operands in computer instruction words. We have simulated the circuit by using Josephson circuit simulation tools. We used simulation tools of XIC, $WRspice^{TM}$, and Julia. To make the circuit work faster, we used a forward clocking scheme. This required a careful design of timing between clock and data pulses in ALU. The RSFQ 1-bit block of ALU used in constructing the 4-bit ALU was consisted of three DC current driven SFQ switches and a half-adder. By commutating output ports of the half adder, we could produce AND, OR, XOR, or ADD functions. The circuit size of the 4-bit ALU when fabricated was 3 mm x 1.5 mm, fitting in a 5 mm x 5mm chip. The fabricated 4-bit ALU operated correctly at 5 GHz clock frequency. The chip was tested at the liquid-helium temperature.