• Journal of the Korea Society of Computer and Information
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• v.7 no.4
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• pp.89-93
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• 2002

This paper describes an ALU core which is suitable for 32-bit DSP This ALU operates in 32-bit data and occupies the third stage, execution, among 5 stage pipeline structure. The supplied functions of the ALU are arithmetic operations, logical operations, shifting, and so on. For the implementation of this ALU core, each functional block is described by HDL. And the functional verification of the ALU core is performed through HDL simulation. This ALU is designed to use the 32-bit DSP.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.1028-1039
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• 2004

During the past 65 million years, Alu sequences have been amplified through RNA-polymerase IIIderived transcripts, and have reached the copy number of about 1.4 million in primate genomes. They are the largest family among mobile genetic elements in human genome and consist of ten percent of the human genome. Alu sequences are thought to be functionless genetically, but many researchers have proved new function and disease implication. Alu elements make the genome insertional mutation, Alu-mediated recombination events, and unexpected splicing site and change gene structures, protein sequences, splicing motifs and expression patterns. In this review, the structure and origin of Alu, consensus sequences of Alu subfamilies, evolution and distribution of Alu, and their related diseases were described. We also indicated new research direction of Alu elements in relation to evolution and disease.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.540-543
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• 2013

This paper describes an implementation of a unified ALU on multi-core GPGPU based on SIMT architecture. Our unified ALU can operate conditional branch instructions, data movement instructions, integer arithmetic instructions and floating-point arithmetic instructions. Since multi-core GPGPU contains a lot of ALU for parallel processing of various types, the main point of this paper is to design the minimum size ALU by unifying similar processing of each operations on circit level. All instrunctions were tested by making a test program. And we compare this results with results of CPU operations to verify our ALU. Our unified ALU's gate size is approximately 20,000 and the maximum operation frequency is 430MHz.

• Genomics & Informatics
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• v.4 no.1
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• pp.11-15
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• 2006

The aim of this study is to identify hRad21-binding sites in human chromosome, the core component of cohesin complex that held sister chromatids together. After chromatin immunoprecipitation with an hRad21 antibody, it was cloned the recovered DNA and sequenced 30 independent clones. Among them, 20 clones (67%) contained repetitive elements including short interspersed transposable elements (SINE or Alu elements), long terminal repeat (LTR) and long interspersed transposable elements (LINE), fourteen of these twenty (70%) repeats clones had Alu elements, which could be categorized as the old and the young Alu Subfamily, eleven of the fourteen (73%) Alu elements belonged to the old Alu Subfamily, and only three Alu elements were categorized as young Alu subfamily. There is no CpG island within these selected clones. Association of hRad21 with Alu was confirmed by chromatin immunoprecipitation-PCR using conserved Alu primers. The primers were designed in the flanking region of Alu, and the specific Alu element was shown in the selected clone. From these experiments, it was demonstrated that hRad21 could bind to SINE, LTRs, and LINE as well as Alu.

• Genomics & Informatics
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• v.14 no.3
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• pp.70-77
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• 2016

Transposable elements are one of major sources to cause genomic instability through various mechanisms including de novo insertion, insertion-mediated genomic deletion, and recombination-associated genomic deletion. Among them is Alu element which is the most abundant element, composing ~10% of the human genome. The element emerged in the primate genome 65 million years ago and has since propagated successfully in the human and non-human primate genomes. Alu element is a non-autonomous retrotransposon and therefore retrotransposed using L1-enzyme machinery. The 'master gene' model has been generally accepted to explain Alu element amplification in primate genomes. According to the model, different subfamilies of Alu elements are created by mutations on the master gene and most Alu elements are amplified from the hyperactive master genes. Alu element is frequently involved in genomic rearrangements in the human genome due to its abundance and sequence identity between them. The genomic rearrangements caused by Alu elements could lead to genetic disorders such as hereditary disease, blood disorder, and neurological disorder. In fact, Alu elements are associated with approximately 0.1% of human genetic disorders. The first part of this review discusses mechanisms of Alu amplification and diversity among different Alu subfamilies. The second part discusses the particular role of Alu elements in generating genomic rearrangements as well as human genetic disorders.

• BMB Reports
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• v.52 no.3
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• pp.196-201
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• 2019

The Alu element, the most abundant transposable element, is transcribed to Alu RNA. We hypothesized that the PIWI protein regulates the expression of Alu RNA in retinal pigment epithelial (RPE) cells, where accumulated Alu RNA leads to macular degeneration. Alu transcription was induced in RPE cells treated with $H_2O_2$. At an early stage of oxidative stress, PIWIL4 was translocated into the nucleus; however, subsequently it was sequestered into cytoplasmic stress granules, resulting in the accumulation of Alu RNA. An elevated amount of Alu RNA was positively correlated with the disruption of the epithelial features of RPE via induction of mesenchymal transition. Therefore, we suggest that oxidative stress causes Alu RNA accumulation via PIWIL4 sequestration into the cytoplasmic stress granules.

• The Journal of Engineering Research
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• v.2 no.1
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• pp.31-37
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• 1997

This paper describes the design and implementation of 8 bit ALU graphic simulator which helps students who study the structure and operation course of general ALU. ALU of this paper consists of three parts, arithmetic circuit, logic circuit, and shifter. Each of them performs as follows. Arithmetic circuit performs arithmetic operation such as addition, subtraction, 1 increment, 1 decrement, 2's complement, logic circuit performs logic operation such as OR, AND, XOR, NOT, and shifter performs shift operation and transfers the result of circuits of arithmetic, logic to data bus. The instructions which relate to these basic ALU functions was selected from Z80 instructions and ALU circuit was designed with those instructions and this designed ALU circuit was implemented on graphic screen. And all state of this data operation course in ALU was showed by bit and logic gate unit.

• Genomics & Informatics
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• v.2 no.2
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• pp.86-91
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• 2004

Even though it represents $6-13\%$ of human genomic DNA, Alu sequences are rarely found in coding regions. When in exon region, over $80\%$ of them are found in 3' untranslated region (UTR). Pseudogenes are an important component of human genome. Their functions are not clearly known and the mechanism of how they are generated is still debatable. Both the Alu and Pseudogenes are important research problems in molecular biology. mRNA is thought to be a prime source of pseudogene and active research is going on its molecular mechanism. We report, for the first time, that mRNAs containing Alu repeats at 3' UTR has a significantly high correlation with processed pseudogenes, suggesting a possibility that Alu containing mRNAs have a high tendency to become processed pseudogenes. It is known that about $10\%$ of all human genes have been transposed. Transposed genes at 3' UTR without Alu repeat have about two processed pseudogenes per gene on average while we found with statistical significance that a transposed gene with Alu had over three processed Pseudogenes on average. Therefore, we propose Alu repeats as a new and important factor in the generation of pseudogenes.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9797-9800
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• 2014

Background: Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and Southeast Asia. Alu elements are among the most prevalent repetitive sequences and constitute 11% of the human genome. Although Alu methylation has been evaluated in many types of cancer, few studies have examined the levels of this modification in serum from NPC patients. Objective: To compare the Alu methylation levels and patterns between serum from NPC patients and normal controls. Materials and Methods: Sera from 50 NPC patients and 140 controls were examined. Quantitative combined bisulfite restriction analysis-Alu (qCOBRA-Alu) was applied to measure Alu methylation levels and characterize Alu methylation patterns. Amplified products were classified into four patterns according to the methylation status of 2 CpG sites: hypermethylated (methylation at both loci), partially methylated (methylation of either of the two loci), and hypomethylated (unmethylated at both loci). Results: A comparison of normal control sera with NPC sera revealed that the latter presented a significantly lower methylation level (p=0.0002) and a significantly higher percentage of hypomethylated loci (p=0.0002). The sensitivity of the higher percentage of Alu hypomethyted loci for distinguishing NPC patients from normal controls was 96%. Conclusions: Alu elements in the circulating DNA of NPC patients are hypomethylated. Moreover, Alu hypomethylated loci may represent a potential biomarker for NPC screening.

• Progress in Superconductivity and Cryogenics
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• v.7 no.1
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• pp.9-12
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• 2005

We have constructed an RSFQ 4-bit Arithmetic Logic Unit (ALU) in a pipelined structure. An ALU is a core element of a computer processor that performs arithmetic and logic operation on the operands in computer instruction words. We have simulated the circuit by using Josephson circuit simulation tools. We used simulation tools of XIC, $WRspice^{TM}$, and Julia. To make the circuit work faster, we used a forward clocking scheme. This required a careful design of timing between clock and data pulses in ALU. The RSFQ 1-bit block of ALU used in constructing the 4-bit ALU was consisted of three DC current driven SFQ switches and a half-adder. By commutating output ports of the half adder, we could produce AND, OR, XOR, or ADD functions. The circuit size of the 4-bit ALU when fabricated was 3 mm x 1.5 mm, fitting in a 5 mm x 5mm chip. The fabricated 4-bit ALU operated correctly at 5 GHz clock frequency. The chip was tested at the liquid-helium temperature.