• Archives of Pharmacal Research
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• 제21권3호
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• pp.260-265
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• 1998

Angiogenic activity of Aloe vera gel was investigated by in vitro assay. We obtained the most active fraction from dichloromethane extract of Aloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type I collagen gel and form capillary-like tube through in vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel through in vitro invasion assay. Furthermore, the effect on the MRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitory (PAl-1) mRNA was not changed.

• 한방안이비인후피부과학회지
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• 제17권3호
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• pp.153-166
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• 2004

In addition to antiseptic／antibiotic regimens, current therapies such as topical and systemic corticosteroids, anti-histamine agents, immune suppressing agent are generally used in the western medicine to treat atopic dermatitis(AD). However, there is almost a complete lack of effective strategies to achieve long-term control of disease, oriental medical treatments are proposed an alternative measure. It has been proved by experimental and clinical study that Oriental medical atopic dermatitis(AD) treatments such as herb-medicine, acupuncture and moxibustion therapy, and steam therapy are effective in long-term treatment. Nevertheless, oriental medical therapy has some limitations to treat acute stage of the disease. Recently developed No-Hoe(蘆？: Aloe vera L.) extract dressings will be a supplementary methods to the oriental medical therapy. The dressing can subside pruritus, exudation, erythema and scaling in the acute stage of atopic dermatitis(AD). We applied the No-Hoe(蘆？: Aloe vera L.) extract dressings to eight atopic dermatitis patients and got some good effects. This reports will provide an alternative choice for the oriental and westernal medical therapy for long-term AD treatment.

• 대한약침학회지
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• 제23권3호
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• pp.173-178
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• 2020

Objectives: Atopic dermatitis (AD) is a prevalent and chronic, pruritic inflammatory skin condition that can influence all age groups. AD is associated with a poor health-related quality of life. This randomized clinical trial was performed to compare the effectiveness of Olivederma (combination of aloe vera and virgin olive oil) or betamethasone regarding disease severity, quality of life, serum IgE and eosinophil count. Methods: Thirty-six AD patients were randomly allocated to topical Olivederma or betamethasone, and were followed for 6 weeks. Results: Total SCORAD severity scores showed significant decrease in both groups, while it was more prominent in Olivederma group (64.5% improvement in Olivederma vs. 13.5% improvement in Betamethasone, p-value < 0.001). Quality of life (DLQI questionnaire) of AD patients was significantly improved after 6 weeks treatment with Betamethasone (22.3%, p < 0.001) and Olivederma (60.7%, p-value < 0.001). Olivederma group showed a significantly lower DLQI score in comparison with Betamethasone treated patients after 6 weeks of therapy (p < 0.001). Improvements in eosinophil count and serum IgE was observed. Conclusion: In summary, this study shows that Olivederma is superior to topical Betamethasone after 6 weeks of therapy with regard to disease severity, quality of life and eosinophil count.

• 한국식품과학회지
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• 제29권6호
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• pp.1269-1274
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• 1997

국내에서 재배된 Aloe vera에서 생리활성 물질인 acetylmannan을 분리 하고 그 제반의 특성을 규명하고 ACE저해효과 및 동력학적분석을 행한 결과 다음과 같은 결론을 얻었다. 알로에 베라 3.5 Kg으로 부터 에탄올에 불용성 다당체를 수득, 백색의 분말형태인 0.9 g의 acetylmannan을 얻었다. 분리한 acetylmannan은 탄수화물 67%를 함유하고 있으며 만노오즈는 60% 소량의 글루코오즈와 갈락토오즈로 이루어져 있고 아세틸기는 23%를 함유하고 있으며 acetyl group은 IR 스펙트럼에서 $1746\;cm^{-1}$, $1244\;cm^{-1}$ peak으로서 확인하였다. 과요오드 산화법에서 과요오드 음이온 소비량이 포름산 생성량에 비해 많고, 1, 4 글리코사이드 결합으로 이루어져 있음을 확인하였다. 또한 ACE에 대한 $IC_{-50}$ 값은 0.58 mM 이였으며, competitive 저해형식을 나타내었고. Ki 값은 0.068 mM 이였다.

• 한국자원식물학회지
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• 제30권3호
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• pp.280-286
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• 2017

Barbaloin is a major component of Aloe vera, which has been used for a laxative. Also, barbaloin is C-glucoside of aloe emodin anthrone which is founded in Aloe vera. Barbaloin has varieties of pharmacological activity such as inhibitory effects on inflammation, histamine release, cancer and microbial infection. But the effect of barbaloin on lipopolysaccharide (LPS)-stimulated macrophages has not been understood. In this study, we evaluated the effects of barbaloin against LPS-stimulated production of nitric oxide (NO), inflammatory cytokines and MAPKs activation in macrophage. We treated barbaloin (0.1, 1, 10, $100{\mu}M$) in LPS-stimulated mice peritoneal macrophage. Our results showed that barbaloin significantly inhibited production of NO and cytokines of tumor necrosis factor $(TNF)-{\alpha}$, interleukin (IL)-6, interleukin $(IL)-1{\beta}$ in LPS-stimulated peritoneal macrophage. Moreover, barbaloin inhibited the phosphorylation of ERK and JNK in a dose dependent manner. These results indicated that barbaloin could be useful for inflammatory diseases.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.308.2-308.2
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• 2002

Tissue macrophages produce at least two groups of protein mediators of inflammation. interleukin 1(IL-1) and tumor necrosis factor (TNF) when they were activated. Recent studies have emphasized that TNF and IL-1modulate the inflammatory function of endothelial cells. leukocytes. and fibroblasts, Aloe vera has been claimed to have several important therapeutic properties including acceleration of wound healing, immune stimulation, anti-cancer and anti-viral effects. (omitted)

• 한국식품과학회지
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• 제31권4호
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• pp.899-905
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• 1999

Aloe vera의 점액성의 젤리 및 녹색의 표피로부터 gel chromatography 및 친화크로마토그래피를 이용하여 렉틴을 분리하여 SDS-PAGE에 의하여 분자량을 확인하였다. 젤리로부터 분리된 물질을 전기영동한 결과, Sephadex G-100으로 정제한 경우, 58.7 kD와 33.3 kD의 분자량에서 band가 나타났고, 산처리한 Sepharose 4B column으로 정제한 렉틴의 경우에는 176.4 kD의 band가 나타났다. 표피로부터 분리된 물질을 전기영동한 결과, Sephadex G-100으로 정제한 경우, 221.1 kD, 54.0 kD, 32.5 kD에서 넓은 band가 나타났으며, 산처리 한 Sepharose 4B column으로 정제한 경우에는 222.0 kD 및 158.0 kD의 band가 나타났다. 산처리한 Sepharose 4B로부터 분리된 렉틴에 대한 당의 적혈구 응집력 억제효과를 측정한 결과, 젤리의 경우, D-galactose, lactose, D-galactosamine과 특이성이 있는 것으로 나타났으며, 그 중 lactose와 가장 큰 특이성이 있는 것으로 나타났다. 그러나, 표피의 경우에는 D-galactose, D-galactosamine에만 특이성이 있는 것으로 나타났으며, 젤리와는 달리 lactose와는 특이성이 없는 것으로 나타났다. 친화크로마토그래피로부터 분리된 렉틴에 대한 pH의 영향을 측정한 결과, 젤리와 표피로부터 얻은 시료 모두 pH $7.0{\sim}9.0$의 pH 범위에서 안정하였다. 또한, 온도의 영향을 측정한 결과, $0{\sim}60^{\circ}C$까지 100% 활성을 나타냈고 $70^{\circ}C$ 이상에서는 활성이 떨어지는 것으로 나타났다.

• Applied Biological Chemistry
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• 제36권4호
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• pp.244-248
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• 1993

건조조건에 의한 알로에의 품종별 및 부위별로 지방산 및 유기산과 약효성분인 barbaloin을 추출 분리정량 하였다. 7종의 지방산 및 6종의 유기산과 barbaloin 성분을 확인한 후 각 품종별, 부위별, 건조온도별로 함량을 비교하였다. 7종의 총지방산량으로 볼때 palmitic, eicosanoic acid 함량이 높았으며, Aloe saponaria가 가장 적었다. 6종의 유기산 총량으로는 malic, citric 및 oxalic acid가 다른 유기산보다 많았으며, Aloe sponaria가 함량이 많았고, Aloe arborescence 변종 1은 적었다. $65^{\circ}C$와 $80^{\circ}C$ 열풍건조시료는 유기산 및 지방산의 함량이 거의 유사하였고, 동결건조한 시료는 열풍건조한 시료보다 함량이 낮았다. Barbaloin의 경우 품종별 함량은 Aloe arborescence 변종 1이 가장 높았으며, 건조조건에 따른 Aloe vera의 barbaloin 성분은 $65^{\circ}C$보다 $80^{\circ}C$의 경우 감소되었고 동결건조가 가장 많은 함량을 나타내었다.

• Archives of Pharmacal Research
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• 제18권5호
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• pp.332-335
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• 1995

The angiogenic activity of Aloe vera (Aloe baradensis), known as a good healing plant, was investigated. We have extracted and fractionated dichloromethane extract (G1M1D1) and methanol soluble fraction of dichloromethane extract (G1M1D1M1) which contain low-molecular weight substances of Aloe vera gel. G1M1D1 and G1D1M1 fractions induced a radially arranged, spoke-sheel-like vasculature in chick embryo chorioallantoic membrane (CAM) assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay was very similar to that of phorbol 12-myristate-13-acetate (PMA) used as a positive control. The modified CAM assay, e simple and accurate quantitating method, was used to quntitate the angiogenic activity of G1D1M1 fraction. Application of G1M1D1M1 fraction ($100\mug/egg$) resulted in much more intense angiogenesis than in control while slightly less intense angiogenesis than in PMA (100 ng/egg).

• 약학회지
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• 제40권2호
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• pp.177-182
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• 1996

The contents of aloesin in aloe preparations were determined by HPLC. Aloesin was extracted 3 times with ethanol for 30 minutes. The ethanol extract was concentrated and suspend ed in saturated NaCl aqueous solution and successively partitioned with dichloromethane, n-butanol. Prepared samples were analyzed by HPLC on a reverse column(Inertsil ODS-2). In assay, internal standard was a puerarin and regression of calibration curve was 0.998. Recoveries of aloesin added to aloe preparation were 98~123(%).