Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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In vivo Angiogenic Activity of Dichloromethane Extracts of Aloe vera Gel

  • Lee, Myoung-Jin (College of Pharmacy, Taegu Hyosung Catholic University) ;
  • Yoon, Soo-Hong (College of Pharmacy, Taegu Hyosung Catholic University) ;
  • Lee, Seung-Ki (College of Pharmacy, Seoul National University) ;
  • Chung, Myung-Hee (College of Medicine,Seoul National University) ;
  • Park, Young-In (College of Natural Resources, Korea University) ;
  • Sung, Chung-Ki (College of Pharmacy, Chonnam National University) ;
  • Choi, Jae-Sue (Department of Food and Nutrition, National Fisheries University of Pusan) ;
  • Kim, Kyu-Won (Department of Molecular Biology, Pusan National University)
  • Published : 1995.10.01
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Abstract

The angiogenic activity of Aloe vera (Aloe baradensis), known as a good healing plant, was investigated. We have extracted and fractionated dichloromethane extract (G1M1D1) and methanol soluble fraction of dichloromethane extract (G1M1D1M1) which contain low-molecular weight substances of Aloe vera gel. G1M1D1 and G1D1M1 fractions induced a radially arranged, spoke-sheel-like vasculature in chick embryo chorioallantoic membrane (CAM) assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay was very similar to that of phorbol 12-myristate-13-acetate (PMA) used as a positive control. The modified CAM assay, e simple and accurate quantitating method, was used to quntitate the angiogenic activity of G1D1M1 fraction. Application of G1M1D1M1 fraction ($100\mug/egg$) resulted in much more intense angiogenesis than in control while slightly less intense angiogenesis than in PMA (100 ng/egg).

Keywords

References

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