• 생약학회지
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• 제33권4호통권131호
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• pp.296-300
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• 2002

The contents of alkaloids in raw Cho O(Aconiti Ciliare Tuber) and its processed products were determined by high performance liquid chromatography. Processing methods 1 and 2 lowered the contents of alkaloids more than the method 3. Cho O processed with method 1 or 2 showed even lower alkaloid contents than commercially available Processed Aconiti Tuber Powder(PATP). The contents of aconitine and hypaconitine in MeOH extracts of Cho O treated with method 3(10 min irradiation) and mesaconitine of Cho O treated with method 3(5 min) were comparable to those of PATP.

• 생약학회지
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• 제17권1호
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• pp.49-54
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• 1986

To explore the biological activities of Thalictrum alkaloids, a study was initiated to investigate the alkaloidal constituents from T. fauriei, a species indigenous to Taiwan. Extensive fractionation of the ethanolic extract provided three known aporphines, (+)-oconovine (Ia), (+)-isocorydine (Ib), and (+)-corydine (Ic) from nonphenolic tertiary base fraction. From quaternary base fraction two new protoberberinium salts, thalifaurine (IIa), dehydrodiscretine (IIb), in addition to magnoflorine (III), were isolated. The structure of both new compounds were confirmed by total syntheses. Fractionation of phenolic bases yielded a dihydromorphinandienone, (-)-ocobotrine (IV), as well as a novel aporphine-pavine dimer, tentatively named EP-10. The structure of EP-10 was established by means of 2D NMR studies. Preliminary study indicated a weak activity of lyzing Hela cells, in vitro, for EP-10.

• 생약학회지
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• 제2권1호
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• pp.19-21
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• 1971

Examination of the alkaloids in the stem of Berberis amurensis $R_{UPRECHT}$ var. latifolia $N_{AKAI}$ was carried out. As tertiary bases, the biscoclaurin type base berbamine and oxyberberine as the berberine type were isolated, and jatrorrhizine, berberine, shobakunine as the berberine type and the aphorphine type magnoflorine were obtained as the quarternary bases.

• 생약학회지
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• 제37권1호통권144호
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• pp.60-66
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• 2006

Norditerpene alkaloids are the main toxic principles of traditional oriental herb 'chuawu(bu-shi),' which have been used in Chinese materia medica mainly for the treatment of musculoskeletal disorders. They are biosynthesized via cyclization of geranylgeranyl pyrophosphate, incoration of 2-aminoethanol and decarboxylation not via amino acid pathway, and the structural characteristics have tempted several oxidative reactions. In this study various norditerpene alkaloids were subjected to react active manganese dioxide and oxoammonium salt as oxidants. The oxidation was proceeded as dealkylation with aconitine type and dehydrogenations with lycoctonin and heteratisine type.

• 약학회지
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• 제11권1_2호
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• pp.4-6
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• 1967

Cation exchange resin particles amberlite IRC-50 were used as a medium for a identific test of alkaloids with dragendorff reagent. The procedures were as follows; A few particles of amberlite IRC-50 were added to the small portion of sample solutions on a spot plate or in a test tube. After 20 minutes-an hour, a drop of dragendorff reagent were added to particles of resin that were adsorbed. When alkaloid was present, the original color of the resin particles changed instantly to red or reddish orange depending on the amount and kind of alkaloid, while in the absence of alkaloid the original color of the resin particles changed light yellow. These methods were more sensitive than the ordinary spot test or paper spot test for alkaloid. The limits of identifications of nine alkaloids were tested by these methods and compared with the paper spot test method.

• Bulletin of the Korean Chemical Society
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• 제31권11호
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• pp.3301-3303
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• 2010

Two new diterpenoid alkaloids, N(19)-en-denudatine (1) and N(4)-butanone-flavaconitine (2), were isolated from Aconitum brachypodum Diels.. Their structures were elucidated by comprehensive spectroscopic analyses including UV, IR, MS, 1D- and 2D-NMR.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.679-682
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• 2002

The alkaloids, piperlongumine, piperine, pipernonaline, and piperoctadecalidine, isolated from Piper longum L., were found to inhibit the biosynthesis of aflatoxin $B_1$ (AF$B_1$) in Aspergillus flavus WRRC 3-90-42-12. Piperlongumine was the most active among the compounds tested, with a 96% inhibition of AF$B_1$biosynthesis at 0.2% (w/v) supplement in a potato dextrose agar (PDA) medium. The three other piperidine alkaloids, pipeline, pipernonaline, and piperoctadecalidine, also inhibited the biosynthesis of AF$B_1$. Of these three alkaloids, piperoctadecalidine exhibited a potent inhibitory activity with a 100% inhibition of AF$B_1$ production at 0.7% (w/v) supplement in a PDA medium. Therefore, piperlongumine and piperoctadecalidine could be used as antiaflatoxigenic agents in agricultural industries. To determine the antiaflatoxigenic mode of action of piperlongumine, further studies are needed.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.393-397
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• 1994

Rhomobifoline and 5,6-dehydrolupanine were isolated for the frist time from the leaves and stems of A. foetida L. indigenous to Saudil Arabia. In addition, five other alkaloids, previously identified in A, foetidia L., namely N-methylcytisine, sparteine, anagyrine, lupanine and cytisine, were isolated. The isolated alkaloids were characterized by UV, $^1-NMR,{\;}^{13}H-NMR,{\;}^{13}C-NMR{\;}and{\;}Mass{\;}spectral{\;}data.{\;}^{13}C-NMR$ data of rhombifoline and 5,6-dehydroupanine are reported for the first time.

• 한국자원식물학회지
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• 제7권1호
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• pp.89-95
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• 1994

Antileukemic tropoloisoquinoline alkaloids, pareirubine A (1) and gandirubrine (2), at first have been isolated from.Abuta concolor (Menisper-maceae). Methylation of 1 and 2, existing in solution as a mixture of tautomersgave the corresponding four methyl derivatives (3 -6). Thioimerubrine (7) andthioisoimerubrine (8) were prepared by the nucleophilic substitutions of themethoxyl groups at C-11 and -10, respectively. Acetylation of 1 and 2produced the corresponding mono-acetyl tautomers (9 and 10). Antileukemicactivity of these derived tropoloisoquinoline alkaloids is also reported.

• Archives of Pharmacal Research
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• 제24권3호
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• pp.202-206
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• 2001

The effects of various sedative cyclopeptides and peptide alkaloids from the Zizyphus species on calmodulin-dependent $Ca^{2+}$ -ATPase and phosphodiesterase were Investigated. Calmodulin-induced activation of $Ca^{2+}$-ATPase was strongly inhibited by sanjoinine-A dialdehyde (IC_{50}$, 2.3$\mu\textrm{m}$), -Ah1 (IC_{50}$, 4.0$\mu\textrm{m}$), -A (IC, 4.6$\mu\textrm{m}$), and -G2 (IC_{50}$, 7.2$\mu\textrm{m}$), while calmodulin-induced activation of phosphodiesterase was strongly inhibited by both deachuine- S10 (IC_{50}$, 4.9$\mu\textrm{m}$) and sanjoinine-D (IC_{50}$, 9.0$\mu\textrm{m}$). The inhibitory activity of the various cyclopeptides and peptide alkaloids on $Ca^{2+}$-ATPase was found to correlate well with their Sedative activity.