The microdetection of alkaloids with amberlite IRC-50

Amberlite IRC-50에 의한 alkaloid의 미량검출법

Cation exchange resin particles amberlite IRC-50 were used as a medium for a identific test of alkaloids with dragendorff reagent. The procedures were as follows; A few particles of amberlite IRC-50 were added to the small portion of sample solutions on a spot plate or in a test tube. After 20 minutes-an hour, a drop of dragendorff reagent were added to particles of resin that were adsorbed. When alkaloid was present, the original color of the resin particles changed instantly to red or reddish orange depending on the amount and kind of alkaloid, while in the absence of alkaloid the original color of the resin particles changed light yellow. These methods were more sensitive than the ordinary spot test or paper spot test for alkaloid. The limits of identifications of nine alkaloids were tested by these methods and compared with the paper spot test method.