• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1369-1378
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• 2007

For effective exopolysaccharide production and mycelial growth by a liquid culture of Fomitopsis pinicola in an air-lift bioreactor, the culture temperature, pH, carbon source, nitrogen source, and mineral source were initially investigated in a flask. The optimal temperature and pH for mycelial growth and exopolysaccharide production were $25^{\circ}C$ and 6.0, respectively. Among the various carbon sources tested, glucose was found to be the most suitable carbon source. In particular, the maximum mycelial growth and exopolysaccharide production were achieved in 4% glucose. The best nitrogen sources were yeast extract and malt extract. The optimal concentrations of yeast extract and malt extract were 0.5 and 0.1%, respectively. $K_2HPO_4\;and\;MgSO_4{\cdot}7H_2O$ were found to be the best mineral sources for mycelial growth and exopolysaccharide production. In order to investigate the effect of aeration on mycelial growth and exopolysaccharide production in an air-lift bioreactor, various aerations were tested for 8 days. The maximum mycelial growth and exopolysaccharide production were 7.9 g/l and 2.6 g/l, respectively, at 1.5 vvm of aeration. In addition, a batch culture in an air-lift bioreactor was carried out for 11 days under the optimal conditions. The maximum mycelial growth was 10.4 g/l, which was approximately 1.7-fold higher than that of basal medium. The exopolysaccharide production was increased with increased culture time. The maximum concentration of exopolysaccharide was 4.4 g/l, which was about 3.3-fold higher than that of basal medium. These results indicate that exopolysaccharide production increased in parallel with the growth of mycelium, and also show that product formation is associated with mycelial growth. The developed model in an air-lift bioreactor showed good agreement with experimental data and simulated results on mycelial growth and exopolysaccharide production in the culture of F. pinicola.

• 한국미생물·생명공학회지
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• 제17권6호
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• pp.624-628
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• 1989

A study on the citric acid production using Saccharomycopsis lipolytica (NRRL Y7576) was carried out in shake-flasks, air-lift and membrane recycle bioreactors. The cells entrapped in Ca-alginate beads were used in shake-flasks and air-lift reactor. Repeated batch fermentation in shake-flasks was successfully performed for 34 days and resulted in a yield of 54%. Increased yield (63%) was obtained in the air-lift reactor operation using nitrogen deficient medium (NDM). In the membrane recycle bioreactor operation, the maximal dry cell mass concentration was 39 g/1 at a dilution rate of 0.02 h$^{-1}$ and the yield with NDM was higher than that with growth medium. In addition, the yield and volumetric productivity with pure oxygen supply were greatly improved compared with those with air supply.

• Journal of Plant Biotechnology
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• 제31권3호
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• pp.249-253
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• 2004

생물반응기의 형태에 따른 인삼부정근의 생장과 ginsen-side의 생산능력에 대하여 조사한 결과, 원형의 상부와 하부에 5 cm의 bubble column을 가진 bulb type bubble bioreactor (BU)에서 건물중은 41.92 g으로 가장 많이 증가하였으며, cylindric tube bioreactor (CT)에서 건물중이 38.55g으로 가장 낮게 나타났다. 이들 두 생물반응기의 초기 kLa 값은 BU 생물반응기에서 6.98 h$^{-1}$로 가장 높게 측정되었고, 반대로 bubble column이 없는 CT 생물반응기에서 5.25 h$^{-1}$으로 가장 낮게 측정되었다. 그러나 이와 같은 초기 kLa값의 차이는 부정근내의 이차대사산물인 ginsenoside의 함량에는 영향을 주지 않는 것으로 나타났다. 또한 BU 생물반응기에서 bubble column의 길이를 기존의 5 cm에서 10 cm로 연장시켰을 때, 초기 kLa값이 6.52 h$^{-1}$에서 7.80 h$^{-1}$로 증가하면서 인삼부정근의 생장을 42.13 g에서 50.30 g으로 약 16% 증가시킨 것으로 나타났다.

• Journal of Applied Biological Chemistry
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• 제43권2호
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• pp.117-118
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• 2000

• KSBB Journal
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• 제8권3호
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• pp.193-199
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• 1993

공기부양 생물반응기에서 천연 청색 색소원인 쪽 (Poly,앵wm tinctorium) 세포를 대량 배양하기 위한 배양조건을 찾기 위해 영양성분 빛 색소 전 구체를 포함한 배지 성분의 생육에 미치는 영향 과 여러 배양조건에서 세포의 생육 특성에 대하 여 설험하였다. 최적 생육조건과 생육 특성을 규 명하기 위 해 external 1oop와 internal loop 두 가지의 arr-lift bioreactor 종류를 사용하여 14일간 배양하였다. 초기 접종량을 달리한 결과 각 reactor에서 공히 접종량이 0.5-2.5% packed cell volume 보다 많을수록 생육에 좋은 결과를 나타내였다. 초기 당농도의 영향을 살펴보기 위하여 2-4%로 그 조건을 달리하여 14일간 배양한 결과 4% 초기 당농도가 생육에 가장 좋은 조건임을 알 수 있었다. Sucrose는 대수기 초기단계에서 모두 소비되었다. Tryptophan(lmM)을 첨가한 경우 14일 배양 후에 internal loop reactor에서 3.8g/l, external loop reactorr에서 3.5g/l 의 cell mass를 얻었다. Indole을 첨가했을 때에는 세포 생육 저해효과 를 나타내였는데 external과 internal Ioop reactor에서 각각 2.5g/l와 2.2g/l를 얻었다. 무기질소원 으로는 potassium nitrate가 선발되었고, contrl이에 비해 110%d의 생육도 증가가 있였다. 최적 조건 하에셔의 14일간 internal loop bioreactor 배양에서 세포 건조 중량 16.34 g/l를 얻었다.

• 환경위생공학
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• 제23권2호
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• pp.27-33
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• 2008

Using crude soybean oil as the sole carbon source, the lincomycin production from Streptomyces lincolnensis LC 345 was investigated in the air lift bioreactor. When 30 g/L of crude soybean oil was used, the maximum lincomycin concentration reached 0.89 g/L, after 5 days of culture. When CSL concentration was increased from 10 to 30 g/L, Lincomycin concentration was increased from 0.6 to 1.2. On the other hand, when CSL concentration was increased from 40 to 60 g/L, it was decreased from 1.15 to 0.7 g/L. Using these results, fed batch cultures for comparing the use of crude soybean oil and glucose as a conventional carbon source were carried out in a 5 L air lift bioreactor. When crude soybean oil was used as the sole carbon source, the maximum lincomycin concentration was 2.0 g/L, which was about 2.0 fold higher than that of glucose medium after 7 day of culture. The product yield from olive oil was 0.042 g/g consumed carbon source, which was about 3.8 fold higher than that of glucose.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.728-732
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• 2000

Propagation of Anagrapha falcifera nuclear polyhedrosis virus(AfNPV) was investigated using well-plates and split-flow air-lift bioreactors. In well-plate experiments, the effects of pH, cell density at a point of infection, serum concentration, DEAE-dextran, and lipid on virus propagation were all closely examined. The AfNPV titer in well-plates was optimal at pH 6.8 and $3{\times}10^6$ cells/$cm^2$. The virus titer was not dramatically affected when the fetal bovine serum concentration was reduced from 10% to 5%. The addition of cholesterol at AfNPV infection of Sf21 cells enhanced the virus titer, whereas the addition of DEAE-dextran did not improve the titer. The AfNPV titer ($3.8{\times}10^7$ $TCID_{50}/ml$) at optimized conditions for well-plate experiments was 2.5-fold higher than for the control. In bioreactor experiments, the AfNPV titer showed its maximum level at air flow rates of 20-40 ml/min. In a split-flow air-lift bioreactor, AfNPV titer ($2.3{\times}10^7\;TCID_{50}/ml$) was 1.5-fold higher than the control when the culture was at pH 6.8 and supplemented with 0.34 mM cholesterol.

• Journal of Plant Biotechnology
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• 제31권3호
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• pp.197-201
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• 2004

자구를 형성 및 비대시키기 위하여 BA 0.5 mg/L와 IAA 0.5 mg/L가 첨가된 MS 배지에서 Lilium longiflorum 'Geogia'의 신초를 증식하였다. 증식된 신초 cluster를 5 L air lift 생물반응기에 배양하였다. 자구비대는 sucrose 60 g/L 및 1∼2배 사이의 MS 배지 염류농도에서 효과적이었다. 재료 투입량은 100 g을 투입하는 것이 자구형성 및 자구비대에 적절하였다. 배양방법은 air-lift 방법이 자구의 형성 및 비대를 촉진하였으며, 주입공기량은 200∼300 mL$.$$min^{-1}$을 주입하는 것이 자구비대에 효과적이었다.

• 한국물환경학회지
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• 제22권1호
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• pp.45-50
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• 2006

This study was performed to evaluate the air lift reactors (ALR) by variations of HRT and recycling rate. Air lift reactor was composed of bioreactor and clarifier above it. To remove organic matters and nitrogen through the formation of microbic film and filtration, bio-filter reactors were filled with clay, glass, bead, waste plastic, respectively. Influent wastewater was fed to biofilter reactor, and effluent wastewater from bio-filter reactor was injected ALR again, instead of adding external carbon source. Effluent BOD concentration was satisfied with lower than 10 mg/L in recycling rate 100% regardless of the variation of HRT and the kinds of media materials. In HRT 4 hr, recycling rate 100%, BOD removal efficiency rate was from about 85 to 90%, COD removal efficiency rate was higher than 90%. Effluent TN concentration was satisfied with less than 20 mg/L, if HRT was maintained by over than 6 hr regardless of recycling rate and media materials. Over than HRT was 4 hr, microbes concentration in air lift reactor was maintained over than 2,500 mg/L constantly, not sensitive to environmental condition, and organic removal was effective as it was higher.

• 한국균학회지
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• 제34권1호
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• pp.1-6
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• 2006

잔나비불로초 배양에 적합한 생물반응기를 선발하기 위하여 4종류의 생물반응기 형태별 균사체 생장특성과 다당체 생산량을 비교하였다. 균사체 생산량은 기포통기형 생물반응기($7\;g/{\ell}$) > 풍선형 생물반응기($6.2\;g/{\ell}$) > 교반형 생물반응기($6\;g/{\ell}$) > 컬럼형 생물반응기($5\;g/{\ell}$) 순으로 나타났으며, 기포통기형 생물반응기에서 가장 높은 세포외 다당체($1\;g/{\ell}$)와 세포 내다당체(2.7%)를 생산하여 기포통기형 생물반응기가 잔나비불로초 균사체 배양에 가장 적합한 것으로 나타났다. 세포외 다당체의 분자량도 생물반응기의 형태별에 따라 다르게 나타났으며, 기포통기형 생물반응기에서 가장 높은 분자량을 나타냈다. 기포통기형 생물반응기에서의 적정한 공기공급율은 0.05부터 0.1 vvm 사이로 나타났다. 특히 잔나비불로초 균사체는 배양하면서 배지의 점도가 높아져 점차 공기 공급량을 높여 주는 것이 좋은 것으로 판단된다.