Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)

다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향

  • 김윤수 (중앙대학교 인삼산업연구센터) ;
  • 한은주 (충북대학교 첨단원예기술개발연구센터) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

생물반응기의 형태에 따른 인삼부정근의 생장과 ginsen-side의 생산능력에 대하여 조사한 결과, 원형의 상부와 하부에 5 cm의 bubble column을 가진 bulb type bubble bioreactor (BU)에서 건물중은 41.92 g으로 가장 많이 증가하였으며, cylindric tube bioreactor (CT)에서 건물중이 38.55g으로 가장 낮게 나타났다. 이들 두 생물반응기의 초기 kLa 값은 BU 생물반응기에서 6.98 h$^{-1}$로 가장 높게 측정되었고, 반대로 bubble column이 없는 CT 생물반응기에서 5.25 h$^{-1}$으로 가장 낮게 측정되었다. 그러나 이와 같은 초기 kLa값의 차이는 부정근내의 이차대사산물인 ginsenoside의 함량에는 영향을 주지 않는 것으로 나타났다. 또한 BU 생물반응기에서 bubble column의 길이를 기존의 5 cm에서 10 cm로 연장시켰을 때, 초기 kLa값이 6.52 h$^{-1}$에서 7.80 h$^{-1}$로 증가하면서 인삼부정근의 생장을 42.13 g에서 50.30 g으로 약 16% 증가시킨 것으로 나타났다.

Keywords

References

  1. Bartholomew WH, Karrow EO, Sfat FR, Wilhelm RH (1950) Oxygen transfer and agitation in submerged fermentations. Mass transfer of oxygen in submerged fermentations of Streptomyces griseus. Ind Eng Chem 42: 1801-1809 https://doi.org/10.1021/ie50489a032
  2. Choi SM, Son SH, Yun SR, Kwon OW, Seon JH, Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in bioreactor system. Plant Cell Tiss Org Cult 62: 187-193 https://doi.org/10.1023/A:1006412203197
  3. Hahn EJ, Kim YS, Yu KW, Jeong CS, Paek KY (2003) Adventitious root cultures of Panax ginseng C.A. Meyer and ginsenoside production through large-scale bioreactor system. J Plant Biotechnol 5: 1-6
  4. Heijnen JJ, Van't Riet K (1984) Mass transfer, mixing and heat transfer phenomena in low viscosity bubble column reactors. Chem Eng J 28: B21-B42 https://doi.org/10.1016/0300-9467(84)85025-X
  5. Kato A, Shimizu Y, Nagai S (1975) Effect of initial kLa on the growth of tobacco cells in batch culture. J Ferment Technol 53: 744-751
  6. Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H, Surh YJ (2000) Antioxidant and anti-tumor promoting activities of the methanol extract of heat- processed ginseng. Cancer Lett 150: 41-48 https://doi.org/10.1016/S0304-3835(99)00369-9
  7. Kim YS (2002) Production of ginsenosides through bioreactor culture fo adventitious roots in ginseng (Panax ginseng C.A. Meyer). Ph D. thesis. Chungbuk National University, Cheongju, Korea
  8. Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng C.A. Meyer. In Vitro Cell Dev Biol Plant 39: 245-249 https://doi.org/10.1079/IVP2002397
  9. Kevers C, Jacques Ph, Thonart Ph, Gaspar Th (1999) In vitro cultures of Panax ginseng and P. quinquefolium. Plant Growth Regul 27: 173-178 https://doi.org/10.1023/A:1006266413919
  10. Liu CZ, Guo C, Wang YC, Ouyang F (2003) Comparison of various bioreactors on growth and artemisinin biosynthesis of Artemisia annua L. shoot cultures. Process Biochem 39: 45-49 https://doi.org/10.1016/S0032-9592(02)00294-7
  11. Merchuk JC (1990) Why use air-lift bioreactor? Trends Biotechnol 8: 66-71 https://doi.org/10.1016/0167-7799(90)90138-N
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Pan ZW, Wang HQ, Zhong JJ (2000) Scale-up study on suspension cultures of Taxus chinensis cells for production of taxane diterpene. Enzyme Microb Tech 27: 714-723 https://doi.org/10.1016/S0141-0229(00)00276-3
  14. Ryu HW, Chang YK, Kim SD (1994) Airlift bioreactors. Korean J Biotechnol Bioeng 9: 347-364
  15. Rotshteyn Y, Zito SW (2004) Application of modified in vitro screening procedure for identifying herbals possessing sulfonylurea-like activity. J Ethnopharmacol 93: 337-344 https://doi.org/10.1016/j.jep.2004.04.007
  16. Shibata S (2001) Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds. J Kor Med Sci 16: 28-37 https://doi.org/10.3346/jkms.2001.16.S.S28
  17. Sticher O (1998) Getting to the root of ginseng. Chemtech 28: 26-32
  18. Taguchi H, Humphery AE (1966) Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems. J Ferm Technol 44: 881-889
  19. Tanaka H, Nishijima F, Suwa M, Iwamoto T (1983) Rotating drum fermentor for plant cell suspension cultures. Biotechnol Bioeng 25: 2359-2370 https://doi.org/10.1002/bit.260251007
  20. William A, John G, Hendel J (1996) Reversed-phase high- performance liquid chromatographic determination of ginsenosides of Panax quinquefolium. J Chromatogr 775: 11-17
  21. Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. Kor J Plant Tiss Cult 27: 309-315
  22. Zhong JJ, Yoshida M, Fujiyama K, Seki T, Yoshida T (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. J Ferment Bioeng 75: 299-303 https://doi.org/10.1016/0922-338X(93)90155-2
  23. Zhong JJ, Pan ZW, Wang ZY, Wu J, Chen F, Takagi M, Yoshida T (2002) Effect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor. J Biosci Bioeng 94: 244-250 https://doi.org/10.1263/jbb.94.244

Cited by

  1. Effects of Aeration Rate and Sparger Type on Growth and Ginsenoside Accumulation in Bioreactor Cultures of Ginseng Adventitious Root(Panax ginseng C.A. Meyer) vol.32, pp.2, 2005, https://doi.org/10.5010/JPB.2005.32.2.111
  2. Tools for biotechnological production of useful phytochemicals from adventitious root cultures vol.15, pp.1, 2016, https://doi.org/10.1007/s11101-014-9391-z
  3. Ginsenosides: prospective for sustainable biotechnological production vol.98, pp.14, 2014, https://doi.org/10.1007/s00253-014-5801-9