Journal of environmental and Sanitary engineering (환경위생공학)

Korean Society for Environmental Sanitary Engineers (대한환경위생공학회)
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Lincomycin Production in the culture of Streptomyces lincolnensis using crude soybean oil in air lift bioreactor

  • Cho, Ki-An (Department of Environmental health, Cho-dang University) ;
  • Cho, Hoon (Department of Polymer Science & Engineering, Chosun University)
  • Published : 2008.06.30
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Abstract

Using crude soybean oil as the sole carbon source, the lincomycin production from Streptomyces lincolnensis LC 345 was investigated in the air lift bioreactor. When 30 g/L of crude soybean oil was used, the maximum lincomycin concentration reached 0.89 g/L, after 5 days of culture. When CSL concentration was increased from 10 to 30 g/L, Lincomycin concentration was increased from 0.6 to 1.2. On the other hand, when CSL concentration was increased from 40 to 60 g/L, it was decreased from 1.15 to 0.7 g/L. Using these results, fed batch cultures for comparing the use of crude soybean oil and glucose as a conventional carbon source were carried out in a 5 L air lift bioreactor. When crude soybean oil was used as the sole carbon source, the maximum lincomycin concentration was 2.0 g/L, which was about 2.0 fold higher than that of glucose medium after 7 day of culture. The product yield from olive oil was 0.042 g/g consumed carbon source, which was about 3.8 fold higher than that of glucose.

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References

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