Browse > Article

Exopolysaccharide Production and Mycelial Growth in an Air-Lift Bioreactor Using Fomitopsis pinicola  

Choi, Du-Bok (Biotechnology Laboratory, B-K Company Ltd.)
Maeng, Jeung-Moo (Department of Chemical Engineering, Chosun University)
Ding, Ji-Lu (Department of Chemical Engineering, Chosun University)
Cha, Wol-Suk (Department of Chemical Engineering, Chosun University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.8, 2007 , pp. 1369-1378 More about this Journal
Abstract
For effective exopolysaccharide production and mycelial growth by a liquid culture of Fomitopsis pinicola in an air-lift bioreactor, the culture temperature, pH, carbon source, nitrogen source, and mineral source were initially investigated in a flask. The optimal temperature and pH for mycelial growth and exopolysaccharide production were $25^{\circ}C$ and 6.0, respectively. Among the various carbon sources tested, glucose was found to be the most suitable carbon source. In particular, the maximum mycelial growth and exopolysaccharide production were achieved in 4% glucose. The best nitrogen sources were yeast extract and malt extract. The optimal concentrations of yeast extract and malt extract were 0.5 and 0.1%, respectively. $K_2HPO_4\;and\;MgSO_4{\cdot}7H_2O$ were found to be the best mineral sources for mycelial growth and exopolysaccharide production. In order to investigate the effect of aeration on mycelial growth and exopolysaccharide production in an air-lift bioreactor, various aerations were tested for 8 days. The maximum mycelial growth and exopolysaccharide production were 7.9 g/l and 2.6 g/l, respectively, at 1.5 vvm of aeration. In addition, a batch culture in an air-lift bioreactor was carried out for 11 days under the optimal conditions. The maximum mycelial growth was 10.4 g/l, which was approximately 1.7-fold higher than that of basal medium. The exopolysaccharide production was increased with increased culture time. The maximum concentration of exopolysaccharide was 4.4 g/l, which was about 3.3-fold higher than that of basal medium. These results indicate that exopolysaccharide production increased in parallel with the growth of mycelium, and also show that product formation is associated with mycelial growth. The developed model in an air-lift bioreactor showed good agreement with experimental data and simulated results on mycelial growth and exopolysaccharide production in the culture of F. pinicola.
Keywords
Polysaccharide; Fomitopsis pinicola; liquid culture; air-lift bioreactor;
Citations & Related Records
Times Cited By KSCI : 23  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
연도 인용수 순위
1 Angeli-Papa, J. and J. Eyme. 1978. Ultrastructural changes during development of Agaricus bisporus and Agaricus sylvicola, pp. 53-82. In S. T. Chang and W. A. Hayes (eds.), The Biology and Cultivation of Edible Mushrooms. Academic Press, New York
2 Cha, W. S., M. Y. Lee, B. S. Cho, S. Y. Park, and D. G. Oh. 2004. A study of the mycelial growth of Agrocybe aegerita in flask culture. Kor. J. Life Sci. 14: 560-566   DOI
3 Cho, S. M., G. S. Seo, S. H. Yu, I. D. Yoo, and G. C. Shin. 1993. Morphological characterization and culture conditions of a mutant of Ganoderma lucidum. Kor. J. Appl. Microbiol. Biotechnol. 21: 520-526
4 Choi, D. B., H. G. Nam, and W. S. Cha. 2006. Studies on cultivation and biological activities of Pleurotus nebrodensis Inzenga. Kor. J. Chem. Eng. 23: 241-246   DOI   ScienceOn
5 Fang, Q. H. and J. J. Zhong. 2002. Effect of initial pH on the production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum. Process Biochem. 37: 769-774   DOI
6 Jonathan, S. G. and I. O. Fasidi. 2001. Effect of carbon, nitrogen, and mineral source on growth of Pasthyerella atroumbonata. Food Chem. 75: 303-307   DOI   ScienceOn
7 Kim, D. H., B. K. Yang, S. C. Jeong, J. B. Park, S. P. Cho, S. Das, J. W. Yun, and C. H. Song. 2004. Production of a hypoglycemic, extracellular polysaccharide from the submerged culture of the mushroom, Phellinus linteus. Biotech. Lett. 23: 513-517
8 Kim, H. H., J. G. Na, Y. K. Chamg, G. T. Chun, S. J. Lee, and Y. H. Jeong. 2004. Optimization of submerged culture conditions for mycelial growth and exopolysaccharide production by Agaricus blazei. J. Microbiol. Biotechnol. 14: 944-951
9 Kim, J. S., K. Sapkota, S. E. Park, B. S. Choi, S. Kim, N. T. Hiep, C. S. Kim, H. S. Choi, M. K. Kim, H. S. Chun, Y. Park, and S. J. Kim. 2006. A fibrinolytic enzyme from the medicinal mushroom Cordyceps militaris. J. Microbiol. 44: 622-631   과학기술학회마을
10 Lee, J. S., H. S. Baik, and S. S. Park. 2006. Purification and characterization of two novel fibrinolytic proteases from mushroom, Fomitella fraxinea. J. Microbiol. Biotechnol. 16: 264-271   과학기술학회마을
11 Park, J. P., S. W. Kim, H. J. Hwang, and J. W. Yun. 2001. Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production of Cordyceps militaris. Lett. Appl. Microbiol. 33: 76-81   DOI   ScienceOn
12 Park, K. S. and J. D. Lee. 1991. Optimization of media composition and culture conditions for the mycelial growth of Coriolus versicolor and Lentinus edodes. Kor. J. Biotechnol. Bioeng. 6: 91-98
13 Prasad, K. K., S. V. Mohan, Y. V. Bhaskar, S. V. Ramanaiah, V. L. Babu, B. R. Pati, and P. N. Sarma. 2005. Laccase production using Pleurotus ostreatus 1804 immobilized on PUF cubes in batch and packed bed reactors: Influence of culture conditions. J. Microbiol. 43: 301-307   과학기술학회마을
14 Shin, S. E., W. S. Cha, and S. H. Kang. 2003. A study on the mycelial growth of Lentinus lepdeus in liquid culture. Kor. J. Life Sci. 13: 492-497   DOI
15 Xiao, J. H., D. X. Chen, J. W. Liu, Z. L. Liu, W. H. Wan, N. Fang, Y. Xiao, Y. Qi, and Z. Q. Ling. 2005. Optimization of submerged culture requirements for the production of mycelial growth and exopolysaccharide by Cordyceps jingxiensis JXPJ 0109. J. Appl. Microbiol. 96: 1105-1110   DOI   ScienceOn
16 Yoshikawa, K., M. Inoue, Y. Mastsumoto, C. Sakaibara, H. Miyataka, H. Matsumoto, and S. Arihara. 2005. Lanostane triterpenoids and triterpene glycosides from fruit body of Fomitopsis pinicola and their inhibitory activity against COX-1 and COX-2. J. Nat. Prod. 68: 69-73   DOI   ScienceOn
17 Chen, W. C. and C. H. Liu. 1996. Production of $\beta$- fructofuranosidase by Aspergillus japonicus. Enz. Microbiol. Technol. 18: 153-160   DOI   ScienceOn
18 Choi, D. B., W. S. Cha, and S. I. Kim. 2005. Production of heterologous protein from Pichia pasteris by air-lift bioreactor. J. Ind. Eng. Chem. 11: 381-386
19 Kang, T. S., D. G. Lee, and S. Y. Lee. 1977. Isolation and mycelial cultivation submerged of Phellinus sp. Kor. J. Mycol. 25: 257-267
20 Silva, T. M, D. Attili-Angeli, A. F. Carvalho, R. Da Silva, M. Boscolo and E. Gomes. 2005. Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36. J. Microbiol. 43: 561-568   과학기술학회마을
21 Garaway, M. O. and R. C. Evans. 1991. Nutrition as a Basis for the Study of Fungi. John Wiley and Sons, New York, pp. 71-221
22 Bae, J. T., J. Sinha, J. P. Park, C. H. Song, and J. W. Yun. 2000. Optimization of submerged culture conditions for exobiopolymer production by Paecilomyces japonica. J. Microbiol. Biotechnol. 10: 482-487
23 Cha, W. S., D. B. Lee, S. H. Kang, and D. G. Oh. 2003. A study of the culture characteristics of Pholiota nameko mycelium. Kor. J. Life Sci. 13: 498-504   DOI
24 Ling, L. S., R. Mohamad, R. A. Rahim, H. Y. Wan, and A. B. Ariff. 2006. Improved production of live cells of Lactobacillus rhamnosus by continuous cultivation using glucose-yeast extract medium. J. Microbiol. 44: 439-446   과학기술학회마을
25 Mohammad, F. H., A. S. M. Badr-Eldia, O. M. Ei-Tayeb, and O. A. Abdel-Rahman. 1995. Polysaccharide production by Aureobasidium pullulans III. Biomass Bioeng. 8: 121-129   DOI   ScienceOn
26 Wang, Y. C. and B. McNeil. 1995. pH effects on exopolysaccharide and oxalic acid production in cultures of Sclerotium glucanicum. Enzyme Microb. Technol. 17: 124-130   DOI   ScienceOn
27 Choi, D. B., S. Y. Kang, Y. H. Song, K. H. Kwun, K. J. Jeong, and W. S. Cha. 2005. Polysaccharide production in liquid culture of Pleurotus ferulae. J. Microbiol. Biotechnol. 15: 368-374   과학기술학회마을
28 Kim, Y. K., C. L. Xiao, and J. D. Rogers. 2005. Influence of culture media and environmental factors on mycelial growth and pycnidial production of Sphaeropsis pyriputrecens. Mycologia 97: 25-32   DOI   ScienceOn
29 Park, K. H., D. M. Kang, and K. Na. 2006. Physicochemical characterization and carcinoma cell interaction of selforganized nanogels prepared from polysaccharide/biotin conjugates for development of anticancer drug carrier. J. Microbiol. Biotechnol. 16: 1369-1376   과학기술학회마을
30 Cha, W. S., B. S. Cho, and S. Y. Park. 2004. A study on the composition of Cordyceps militaris extract and mycelium. Kor. J. Life Sci. 14: 727-731   DOI
31 Cha, W. S., M. Y. Lee, B. S. Cho, and S. Y. Park. 2004. A study on the composition of seasoning using Lentinus edodes. Kor. J. Life Sci. 14: 829-833   DOI
32 Chang, H. Y., D. Y. Cha, A. S. Kang, I. P. Hong, K. P. Kim, S. J. Seok, Y. J. Ryu, and J. M. Sung. 1995. Cultural characteristics of Fomitella fraxinea. Kor. J. Mycol. 23: 238-245
33 Lee, S. Y. and T. S. Kang. 1996. Production conditions and characterization of the exo-polymer produced by submerged cultivation of Ganoderma lucidum. Kor. J. Appl. Microbiol. Biotechnol. 24: 111-118
34 Lu, S., M. Park, H. S. Ro, D. S. Lee, W. J. Park, and C. O. Jeon. 2006. Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J. Microbiol. 44: 155-161   과학기술학회마을
35 Choi, D. B., K. A. Cho, and W. S. Cha. 2004. Effect of Triton X-100 on compactin production from Penicillium citrinum. Biotechnol. Bioproc. Eng. 9: 171-178   DOI
36 Bae, J. T., J. P. Park, C. H. Song, C. B. Yu, M. K. Park, and J. W. Yun. 2001. Effect of carbon source on the mycelial growth and exopolymer production by submerged culture of Paecilomyces japonica. J. Biosci. Bioeng. 91: 522-524   DOI   ScienceOn
37 Cha, W. S., D. B. Choi, and S. H. Kang. 2004. Optimization of culture media for solid-state culture of Pleurotus ferulae. Biotech. Bioproc. Bioeng. 9: 369-373   DOI
38 Hogberg, N., O. Holdenrieder, and S. Jan. 1999. Population structure of the wood decay fungus Fomitopsis pinicola. Heredity 83: 354-360   DOI   ScienceOn
39 Kim, J. M., K. S. Ra, D. O. Noh, and H. J. Suh. 2002. Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from Fammulina veltipes. J. Ind. Microbiol. Biotech. 29: 292-295   DOI   ScienceOn
40 Park, K. S., S. Park, I. C. Jung, H. C. Ha, S. H. Kim, and J. S. Lee. 1994. Production of protein-bound polysaccharides by solid-state fermentation of Coriolus versicolor. Kor. J. Mycol. 22: 184-189
41 Zadrazil, F. 1974. The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cormucopiae, and Pleurotus eryngii. Mushroom Sci. 9: 621-629
42 Lee, K. M., S. Y. Lee, and H. Y. Lee. 1999. Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift bioreactor. J. Biosci. Bioeng. 88: 646-650   DOI   ScienceOn
43 Kim H. M., S. Y. Park, K. S. Ra, K. B. Koo, J. W. Yun, and J. W. Choi. 2006. Enhanced production of exopolysaccharide by fed-batch culture of Ganoderma resinaceum DG-6556. J. Microbiol. 44: 233-242   과학기술학회마을
44 Cha, W. S., H. D. Lee, and J. S. Kim. 2004. On the composition of Morchella esculenta fruit body. Kor. J. Life Sci. 14: 82-90   DOI
45 Krishna Prasad, K., S. Venkata Mohan, Y. Vijaya Bhaskar, S. V. Ramanaiah, V. Lalit Babu, B. R. Pati, and P. N. Sarma. 2005. Laccase production using Pleurotus ostreatus 1804 immobilized on PUF cubes in batch and packed bed reactors: Influence of culture conditions. J. Microbiol. 43: 301-307   과학기술학회마을
46 Lee, J. H., S. M. Cho, K. S. Ko, and I. D. Yoo. 1995. Effect of culture conditions on polysaccharide production and monosaccharide composition in Pheillinus linteus. Kor. J. Mycol. 23: 325-331
47 Kim, Y. R. 2003. Production of polysaccharide by the edible mushroom, Grifola frondosa. Kor. Soc. Mycol. 31: 205-208
48 Nilanonta, C., M. Isaka, P. Kittakoop, P. Palittapongarnpim, and S. Kamchonwongpaisan. 2000. Antimycobacterial and antiplasmodial cyclodepsipeptides from the insect pathogenic fungus Paecilomyces tenipes BBC 1614. Planta Medica 66: 756-758   DOI   ScienceOn
49 Tang, Y. J. and J. J. Zhong. 2004. Modeling the kinetics of cell growth and ganoderic acid production in liquid static cultures of the medicinal mushroom Ganoderma lucidum. Biochem. Eng. J. 21: 259-264   DOI   ScienceOn
50 Kim, D. J., D. H. Ahn, and D. L. Lee. 2005. Effects of free ammonia and dissolved oxygen on nitrification and nitrite accumulation in biofilm airlift reactor. Kor. J. Chem. Eng. 22: 85-90   DOI   ScienceOn
51 Lee, J. S., M. O. Lim, K. Y. Cho, J. H. Cho, S. Y. Chang, and D. H. Nam. 2006. Identification of medicinal mushroom species based on nuclear large subunit rDNA sequences. J. Microbiol. 44: 29-34   과학기술학회마을
52 Lim, K. H., S. W. Park, and E. J. Lee. 2005. Effect of temperature on the performance of a biofilter inoculated with Pseudomonas putida to treat waste-air containing ethanol. Kor. J. Chem. Eng. 22: 922-926   DOI   ScienceOn
53 Manzoni, M. and M. Rollini. 2001. Isolation and characterization of the exopolysaccharide produced by Daedalea aquercina. Biotechnol. Lett. 23: 1491-1497   DOI   ScienceOn