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검색결과 183건 처리시간 0.024초

Analysis of virulence traits of Staphylococcus aureus isolated from bovine mastitis in semi-intensive and family dairy farms

  • Guzman-Rodriguez, Jaquelina J.;Leon-Galvan, Ma. Fabiola;Barboza-Corona, Jose E.;Valencia-Posadas, Mauricio;Loeza-Lara, Pedro D.;Sanchez-Ceja, Monica;Ochoa-Zarzosa, Alejandra;Lopez-Meza, Joel E.;Gutierrez-Chavez, Abner J.
    • Journal of Veterinary Science
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    • 제21권5호
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    • pp.77.1-77.14
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    • 2020
  • Background: Staphylococcus aureus is one of the main microorganisms that causes bovine mastitis, and its well-known virulence characteristics and interactions with the environment are used to aid the design of more efficient therapies. Objectives: To determine whether the virulence traits, such as antibiotic resistance and biofilm-forming and internalization abilities, of S. aureus isolated from bovine mastitis are related to dairy production system types. Methods: The study was performed in the Mexican states of Guanajuato and Michoacan. Semi-intensive dairy farms (SIDFs) and family dairy farms (FDFs) (454 and 363 cows, respectively) were included. The 194 milk samples from mastitis affected quarters were collected and 92 strains of S. aureus were isolated and identified by biochemical and molecular tests. Antibiotic resistance, biofilm and internalization assays were performed on 30 randomly selected isolated strains to determine virulence traits, and these strains were equally allocated to the 2 dairy production systems. Results: All 30 selected strains displayed a high degree of resistance (50%-91.7%) to the antibiotics tested, but no significant difference was found between SIDF and FDF isolates. S. aureus strains from SIDFs had an average biofilm forming capacity of up to 36% (18.9%-53.1%), while S. aureus strains from FDFs registered an average of up to 53% (31.5%-77.8%) (p > 0.05). Internalization assays revealed a higher frequency of internalization capacity for strains isolated from FDFs (33.3%) than for those isolated from SIDFs (6.7%) (p > 0.05). fnbpA gen was detected in 46.6% of FDF strains and 33.3% of SIDF strains, and this difference was significant (p < 0.05). Conclusions: Our findings show that the virulence traits of S. aureus isolates analyzed in this study, depend significantly on several factors, such as phenotype, genotype, and environmental conditions, which are significantly related to dairy production system type and daily management practices.

감리데이터 기반의 민간 건축현장 성과지표 개발 (Development of Performance Indicators on Private Building Construction Sites using Supervisory Report)

  • 성유경;허윤경;이승우;유위성
    • 한국건설관리학회논문집
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    • 제23권6호
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    • pp.65-75
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    • 2022
  • 성과측정은 체계적인 관리와 개선을 위한 중요한 활동이다. 건설산업에서도 성과측정을 위한 지표 개발 등의 다양한 연구가 꾸준히 진행되어왔다. 하지만 성과측정을 위한 데이터 수집에 큰 노력이 필요하여 실제로 성과측정이 수행되는 경우는 적었다. 특히 제도를 통해 건설 프로젝트 데이터를 수집해 온 우리나라 공공부문과 달리 민간부문에서는 프로젝트의 성과를 파악할 수 있는 데이터가 전무한 실정이다. 한편, 우리나라는 건설공사 감리를 통해 건축현장 시공단계에서의 품질, 안전 등을 총체적으로 확인하는 제도를 갖추고 있다. 감리 과정에서 건축현장의 성과를 파악할 수 있는 중요 데이터가 생성되고 감리보고서는 「건축법」에 따라 제출이 의무화되어 있다. 본 연구는 민간 건축현장의 성과측정을 위해 감리보고서의 데이터를 활용하고자 한다. 선행연구와 감리보고서를 분석하여 예비성과지표를 선별하고 전문가 인터뷰를 통해 건축현장의 6가지 성과영역과 15개 성과지표를 도출하였다. 다음으로 전문가 설문조사를 실시하여 성과지표의 데이터 수집 가능성을 검증하였고, 성과영역 및 성과지표의 중요도를 도출하였다. 감리보고서 데이터를 기반으로 도출된 본 연구의 성과지표는 앞으로 민간 건축부문의 성과측정의 실효성을 높일 것으로 기대된다.

볼 밀링에 의한 석영의 결정도 변화와 밀링 매체의 마모의 영향 (Ball-milling Induced Changes in the Crystallinity of Quartz and Wear of Milling Media)

  • 권진중;김훈;이성근
    • 광물과 암석
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    • 제36권2호
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    • pp.95-106
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    • 2023
  • 석영(SiO2)은 지각의 암석을 형성하는 주요 광물 중의 하나이다. SiO2의 원자 구조는 다양한 마찰 과정에서 변화할 수 있다. 비정질화, 수화 및 실리카겔 형성을 수반하는 석영 암석의 마찰 감소는 지진 및 관련 현상에 대한 광물학적 통찰력을 제공한다. 볼 밀링과 회전 전단 실험을 이용하여 상기 현상의 광물학적 기원이 밝혀지고 있다. 본 연구에서는 밀링 과정에서의 다양한 변수를 고려하여 SiO2의 비정질화를 위한 볼 밀링의 최적 실험 조건을 결정하였다. 높은 밀링 속도에서 볼 밀링 시간이 증가함에 따라 SiO2의 결정도는 점차 감소하여 비정질화되었다. 밀링 매체의 마모 정도와 SiO2의 비정질화에 미치는 영향은 서로 다른 밀링 재료(ZrO2, stainless steel)를 사용하여 분석하였다. 밀링 시간이 증가함에 따라 볼의 마모량이 증가하였다. 또한 볼에서 마모된 스테인리스스틸 입자는 비정질화되는 SiO2와 상호작용하여 Si-O-Cr을 형성하는 경향이 있다. 이러한 결과는 SiO2와 같이 비교적 경도가 높은 다양한 물질들의 볼 밀링에 의한 원자 구조의 변화 과정을 이해하고, 다양한 지질학적 마찰 과정을 이해하는 데 도움이 될 것이다.

관절통환자의 통증정도와 통증연관 행위에 관한 연구 (The Pain Behavior of Patients with Joint Pain)

  • 이은옥;한윤복;김순자;이선옥;김달숙;김조자;김광주;김주희;박점희
    • 대한간호학회지
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    • 제18권2호
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    • pp.197-210
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    • 1988
  • The purposes of this study were : 1) to assess the level of pain and to identity the varieties and the degree of pain-related behavior, 2) to measure the level of correlation between the level of pain and the degree of pain- related behavior, 3) to test the correlation between the Korean Pain Rating Scale (KPRS) and Graphic Rating Scale(GRS), and 4) to gather data relevant to the Socio-demographic status of the subjects. The level of pain was measured by KPRS and GRS developed by the researchers. The KPRS consists of three dimensions ; the sensory, the affective and the miscellaneous and the GRS of two separate scales ; the intensity scale and the unpleasantness scale. Of the 2, 025 who had visited orthopedic and neurosurgical out-patients department of 11 university hospitals in various districts of Korea with the episode of Joint pain, 405 subjects were self-selected by responding to the data gathering tools and questionaires mailed. The results are summaried as follows : 1. Maale(217, 53.6%) exceeded female patients(188, 46.4%) in number and the onset of joint pain was more prevalent in the age groups of the 20s and the 30s. 160(39.5%) had been hospitalized for the treatment of, and 87(21.5%) had retired because of the joint pain. 2. Mean pain score measured by KPRS was 128.31 (range; 0-1.344.8) ; mean sensory score was 43.23(range ; 0-645.88%), mean affective score was 46.09(range ; 0- 356.72), and mean miscellaneous score was 39.99(range ; 0-341.68). Mean pain scores measured by GRS were ; sensory intensity score ; 109.1(range ; 0-200) and distress score ; 99.1 (range ; 0-200). 3. The prevalent sites of joint pain revealed to be the right knee joint(203; 50.1%), left knee joint(181(44.7%), left ilium(147, 36.3%), lumbar region (106; 26.2%), hip joint(92; 22.7%) and the ankle(84; 20.7%). 4. The average sleep hour was 6.8hours per day and the average rest hour during the day hours was 3.3hours (range 0-20). 5. The average duration of suffering from joint pain was 49.1 months. 6. Most of the subjects(298; 73.6%) used some sorts of pain relieving practices ; the most prevalent pain relieving practice was the compliance with the physician prescribed treatments(34.4%). 7. The level of discomfort in carrying out the ADL(activities of daily living was 101.16(38.83) and the level of needs for aid in carrying out the ADL was 76.62(31.79). 8. The interrelation between KPRS total score and GRS sensory intensity score(.4438), as well as that of GRS distress score(r=.4446) were not highly correlated, however, sensory and affective dimension within KPRS (.7547) and pain intensity and distress score of GRS(.6975) revealed moderate intercorrelation. 9. Pain-related behaviors such as discomfort in carrying out ADL, the need for aids in carrying out ADL, frequency of pain relieving practices, varieties of pain sites and length of rest hours during the day hours revealed to be highly correlated with the level of pain measured by KPRS, GRS sensory intensity scale and GRS distress scale. The following are recommended ; 1. Test for the correlation of KPRS total score and the summated score of GRS ; sensory intensity and distress scores. 2. Possibilities of utilization of the pain-related behaviors which revealed high correlation as indirect assessment tool for measuring the level of pain.

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치과건강보험 요양급여비용 청구에 관한 교육요구도 조사 -대전·충청지역을 중심으로- (The investigation of the degree of the request of the education about the claim for the medical expenses in the dentistry health insurance - mainly in the Daejeon, Chungcheong area -)

  • 남용옥;김성희;김민자
    • 한국치위생학회지
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    • 제11권3호
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    • pp.325-341
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    • 2011
  • Objectives : This research has investigated the reality of the education of the claim and the degree of the education for the claimed of the dentistry recuperation organization in the Daejeon and Chuncheong are for the improvement of the problem in the medical expenses. Methods : It use as a basic data for the vitalizations of the education and performed the survey in the dentistry recuperation organization in the Daejeon and ChungCheong Nam BukDo which are registered in the evaluating organization for judging the health insurance in the present May 2010, and concluded just like the below. Results : 1. The education of the claim in the requirer in the dentistry recuperation organization, and the education of the claim was especially lacking when the dentist was studying in the university, and the dental hygienist had the similar educational experience in the school and the clinic (p<0.05) 2. Most of the requirer in the dental recuperation organization was hoping to get the education related to the claim work, but the dentist and the nurse's aid was relatively low (p<0.05) 3. For fixing the error of the claim, the participation and the extension of the judging standard of the insurance was the highest among the university subordinate dental hospital/dental hospital, but the health center was relatively low (p<0.05). 4. The dentist feels the economic burden in employing the special employee because the raising of the special judging people, compared to others, but the staffs such as the dental hygienist preferred it as one of ways to fix the error of the claim of the dental insurance (p<0.05) 5. Both dentists and the dental hygienist said proper time to teach the insurance was all needed in the school, and the clinic, but other workers relatively believed it should be held in the clinic (p<0.05). 6. The important factors to decide the participation of the lecture was in order of the contents of the lecture, the place of the lecture, the amount for the lecture, the superintendent of the lecture, whether it has gone through the educational score, and whether it has passed the conserving educational score was relatively less important in the university subordinate dentist/dentist, but the medical center was very effective as 4.50 (p<0.05) 7. Health Insurance Review and assessment service was very high as the managing department for supplying the lecture and the information, 70.5%, and the next was the Korean Dental Association/ Korean dental hygiene association, but dentists were preferring the association to manage in than the Health Insurance Review and assessment service to manage (p<0.05) 8. In preferring lecture for the inquiring the insurance, periodontal surgery was the highest as 4.51, the diagnosis standard for injection was high in the university subordinate hospital/dentists, and the more the year of the insurance inquiry, the less the doctor who was hoping for the lecture about the basic treatment. Conclusions : Taken together, it is decided that the inquiry education about the medical expense in the dentist, so the consistent and systematic education should be held to the related people, and from this, it is thought to reduce the problem of the inquiry of the medical expenses by fostering the knowledge and supplying the information which are related to the inquiry of the dentists.

개인정보보호 분야의 연구자 네트워크와 성과 평가 프레임워크: 소셜 네트워크 분석을 중심으로 (The Framework of Research Network and Performance Evaluation on Personal Information Security: Social Network Analysis Perspective)

  • 김민수;최재원;김현진
    • 지능정보연구
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    • 제20권1호
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    • pp.177-193
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    • 2014
  • 개인정보 분야에서의 다양한 정보 보안 이슈가 발생함에 따라 해당 분야의 전문가를 확인하기 위한 프레임워크는 매우 중요한 영역이 되었다. 전문가 탐색과정은 주로 연구 업적 등을 통한 주관적인 평가가 일반적이지만 보다 객관적인 방식을 통한 선정이 매우 중요하다. 소셜 네트워크 분석기법의 응용이 다양한 영역에서 활용됨에 따라 본 연구는 개인정보보호분야의 전문가를 확인하고 해당 전문가들의 연구실적을 판단하기 위한 분석 프레임워크를 제시하고자 하였다. 본 연구는 연구 목적에 따라 개인정보보호 연구영역의 연구성과 자료를 바탕으로 소셜 네트워크 분석을 실시하고 핵심연구자의 성과를 분석하였다. 수집된 데이터는 연구의 공저자, 발행기관, 소속기관 등의 네트워크 구성에 활용되어 핵심전문가 집단을 관리하기 위한 프레임워크를 제시하였다. 본 연구는 NDSL에서 최근 5년 동안 발표된 논문들을 중심으로 자료를 수집하였다. 연구자들이 학술 정보를 교환하는 정기 간행물인 학술지를 바탕으로 연구 네트워크를 형성하는 네트워크 자료를 수집함으로써 연구활동에 대한 정보를 분석할 수 있었다. 일반적으로 연구자들은 연구 결과를 논문으로 발표하고, 발표된 논문들이 다수의 관련 분야 전문가들에게 공유된다는 점에서 학술연구지는 연구자들의 지식관련 의사소통 공간이며 지식의 구조화에 핵심적인 역할을 수행한다. 그에 따라 본 연구의 연구 대상 분야로 설정한 개인정보보호 분야의 연구 구조를 이해하기 위해 국내에서 발표된 관련 분야의 논문들을 연구 대상으로 자료가 수집되었다. 특히 자료의 선별 기준은 국내 최대의 데이터베이스를 보유하고 있는 NDSL에서 개인정보보호 관련 키워드를 보유한 논문 데이터를 수집 및 정제하여 분석 자료로 사용하였다. 2005년부터 2013년까지 약 2,000개의 연구결과 중 주제 관련성, 공저자 추출 등을 수집하였다. 데이터 수집 이후 연구 분석을 위한 데이터 처리를 통하여 통해 총 784개의 논문을 선정하고 분석대상으로 확정하였다. 분석 결과, 개인정보보호 연구영역의 전문가 집단을 이용한 연구논문 성과에 대한 분석은 핵심 연구자들을 추출해내고 전문가 집단을 관리하는 데 도움을 제공할 수 있다. 특히 소속집단 및 연구논문 발행기관을 분석함으로써 개인정보보호 연구영역에서 확인되지 않았던 연구자들의 연구 논문 게재의 공저자 네트워크가 매우 밀접함을 확인할 수 있다. 또한 연구논문의 발행기관 및 소속집단의 특성을 추출함으로써 개인정보보호 영역의 전문가 평가지표로서 소셜 네트워크 지표들의 활용가능성을 확인하였다.

청소년들의 건강상태와 건강행위 인지에 관한 연구 (A study of teenager's perceptions of health behavior and health status)

  • 조원정;김모임
    • 보건교육건강증진학회지
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    • 제4권2호
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    • pp.32-45
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    • 1987
  • This research attempted to identify basic data related to the nursing of teenagers that would aid in the establishment of nursing care that would meet their health needs, and further to identify which health related activities, are carried out by teenagers, so that appropriate nursing service and health care can be planned for them. The subjects of the study were students at one seoul boys high school and one seoul girls high school. Data was collected between December 16 and. December 19, 1986. The tool used in the study was a questionnaire about the health needs and concerns of teenagers developed at John's Hopkins University and translated and standardized for Korean students. The date was coded fer analysis using' the SPSS program and percentages, mean scores' with standard deviations, Chi square test, and ANOVA, were used for analysis. The following results were obtained : 1. The health status of teenagers : Looking at the health status of teenagers, it can be seen from this study that over half of the teenagers questioned replied that they do not get enough sleep, further that even though they are feeling sick they go to school. However, when asked what they thought of their health, the majority replied that they were healthy, and although they thought about their bea1th was not a concern when they were planning other activities. With regard to health status, there was a significant difference between male and female students concerning whether they were handicapped with regard to sleep, their opinion about their own health, and the amount of time lost from school for illness over the past month, whether in comparison with those of the same age they had more sickeness of not, and whether they had to think about their health when planning other activities. There was also a significant difference according to the age of the students as to whether they worried more about being sick than other members of their age group. 2. Health Realted Activeties of Teenagers : Most of the students in the study reported that they did not get regular physical examinations of health check ups, nor did they get legular examinations when they were sick. Few students reported that they smoked. Most of the students reported taking part in activities that promoted health but about half also reported that they were involved in activities that they knew were detrimental to their health. Further on statistical analysis, there was a significant difference between male and female students regarding health related activities and problems of teenagers and whether they were examined by a medical person when they were sick. 3. The degree of interest and responsibility of teenagers for their own Health: In the section on responsibility for health, the teenagers replies were highest, 4. 5, for the statements, "My health is dependent how much I pay attention to it" and "My health is responsibility" and lowest, 3. 9, for the statement "I am healthy because I have cared for my health so far." With regard to interest in their own health, the majority of the teenagers in the study were more concerned about their studies, high score of 4. 4, than in matters directly related to health. The use of drugs, birth control and masturbation were low on the interest scale. The more involvement in health related activities, the higher the score was for responsibility and this difference was significant, but this did not apply to interest in health. Similarly there was also a significant difference regarding participation in activities known to be detrimental to health and responsiblity for health, but not for interest in health.

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서울시 고등학생의 안전교육 실태 및 요구도 조사 (Needs Accessment of Safety Education of High School Students in Seoul)

  • 김민아;이명선
    • 보건교육건강증진학회지
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    • 제18권3호
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    • pp.133-162
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    • 2001
  • Unexpected accidents in school has been gradually increased every year, and student's mistaken was the first reason of the accident. To preventing the students from Unexpected accidents in school, safety education is more important than having strong facilities to have much more strong protecting forces for the accidents. Therefore, systematic education of safety is needed most of all, and especially, strengthening safety education in school is needed. Hereby, this study is aimed at investigating and analysing the actual condition and demand of safety education. It also conducted a survey with the target of 1,255 students in the second grade of high school in Seoul from April 20 to May 19, and the result of this study is as follows. 1. In the general features of the subject of the survey, boy students and girl students occupied 50.8% and 49.2% each, and boys' high school (34.3%), girls' high school(32.2%) and co-ed(33.5%) participated in balance. In the location, north from Han river occupied 54.6%, south were 45.2%. 2. According to the status of experiencing an accident, boys were more experienced then girls(p〈0.05). From the section, home accident(56.8%) occupied most followed by school accident, traffic accident, sports accident and poisoning. The accident happening most often in detailed category is sports accident such as basket ball, foot ball and dodge ball. The actual condition of the subject's using a school health facility shows that boys students use it more often than girl students(p〈0.01) 3. In attitude toward safety, the subject showed lower interest in safety issues than other social issues. But attitude in seriality of safety problems were high. Also, they responded ‘individual citizen’(63.1%) as the one who should make efforts for safety. Regarding knowledge of preventing safety accidents in attitude toward individual safety, 42.2% answered ‘they know a little’ and 32.6% of respondents say ‘they do not know’. To a question of the degree of the subject's following safety rules, 36.4% were answered ‘keeping’ and the group using a school health facility shows more ‘keeping’ the others(p〈0.05). 4. To a question of asking if they have experienced safety education, 51.2% answered ‘yes’. Teachers who mainly take care of safety education are answered as training teacher(48.7%). As for education time, training class(51.3%) is said to have safety education most followed by health-related event and PE(Physical Education). Frequency of education shows once or twice a session (62.8%) most often, but in case of co-ed school, 5-7times a session or more(20.1%) are being practiced. Looking at education time, 1-2 hour(s)(22.1%) or for a short time(22.1%) during class are being practiced. As an education method, instruction(43.8%) and video education(32.5%) are being practiced, and when it comes to education evaluation method, ‘not practiced’(70.0%) answered. To the question if they are satisfied with school safety education, they answered more ‘no(43.1%)’ than ‘yes(6.7%)’, and the reason is that safety education class is just for formality's sake, and the fact they already know is being repeated. The contents of safety education is composed of school safety, home safety, and first aid. 5. It is turned out that 56.5% of the total boy students and 61.1% of the total girl students recognize the necessity of safety education. To the question if safety education is needed in an elementary and middle education course, 46.4% of the subject answer answered' it's necessary'. The most reason for their answers are ‘safety education is directly related to life’. 6. Regarding the requested time of safety education is ‘one hour a week’ by 55.9%. For safety education, safety education teachers(38.7%) are answered to be the most proper. As a request for safety education, video education is answered to be the most appropriate(30.6%), followed by practical skills, lecture and discussion(p〈0.05). Demand of educational evaluation, practical skills, interview and observation are answered to be needed. To the question if they want to participate in the way of demanded safety education, 41.9% of respondents answer ‘have a mind to participate in’ (41.9%). To benefits followed by completing safety education, 72.0% of respondents answer ‘agree’, and 24.7% ‘do not agree’, which means lout 4 disagrees with completing safety education. 7. Looking at demand of safety education according to the features of the subject, ‘our position for the person who has handicapped’ was answer to the most.

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두부 손상환자 가족의 간호요구에 대한 연구 (A Study on Needs of the Families of Head Injury Patients)

  • 조규영;박형숙
    • 기본간호학회지
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    • 제6권3호
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    • pp.414-433
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    • 1999
  • The purpose of this study is to identify the needs which are perceived by one of the familiy members who have head injury parients by traffic accidents in the intensive care units. Subjects were 70 families members of head injury patients admitted to 2 general hospitals NICU located Pusan city, 1 general hospital NICU located Ulsan city from December 1. 1998 to February 28. 1999. For this study, 70 family members were interviewed with aid of a Likert scale which was developed by researcher for this study. For development of the survey instrument, literature review and open questionnaire technique with family members and the nurses working in NICU. The 50needs-items were classified into 7 groups according to the homogeneity of the items with the support of literature review. For the content validity, the instrument was reviewed by 1 nursing professor and the internal reliability of this instrument was Cronbach alpha=0.94 which is highly accepted. Data was analyzed by a SPSS computer program. Data analysis included frequency. percentage, mean, standard variance and t-test or ANOVA. The results were as followings : 1. The general characteristics of head injury patients shows that the male was 74.3%, the female was 25.7% and age distribution shows that the fifty-fifty nine years was 30%, the highest. Of religion the buddhism was the most, The diagnosis distribution shows that epidural hematoma was 32.9% and subdural hematoma was 24.3%. The mentalility distribution shows that semicoma was 31.4% and stupor was 31.4%. Hemiplegia was 42.9% 2. The general charaterisrics of the family needs of head injury patients shows that thirty-thirty nine years was 31.4%, the highest. sex distribution shows that the male was 20%, the female was 80%. Of religion the buddism was the most. 3. The family needs of head injury patients was $3.03{\pm}0.42$, needs for the information of a patient's condition was $3.65{\pm}0.48$, the highest. And needs for the information of care and treatment was $3.48{\pm}0.48$, needs to be supplied with comfortable facilities for family was $3.04{\pm}0.66$, needs to be participate in a patient's care was $2.90{\pm}0.55$, needs to be informed about the available resources was $2.83{\pm}0.59$, needs to be supported emotionally for family was $2.79{\pm}0.55$, needs for religious assistance was $2.51{\pm}0.85$. 4. Examining the family needs of head injury patients according to patient's characteristics, mentality, plegia degree and operation were shown be variables to make an effect on the needs for the head injury patients family. At all, according to severity of head. injury, the family needs of head injury patients was high. 5. Examining the family needs of head injury patients according to their general characteristics, we could know that religion, job. income were shown to be variables to make an effect on the family needs. Through the examination it can be seen that the characteristics of head injury patients and the family needs of head injury patients. In conclusion, the family needs of head injury patients was almost same the family needs of ICU patients. Therefore we must involve the family's care of head injury patients and we must provide exact and repeated explanation, education and support the family of head injury patients. As this study was resulted in selecting the families admitted to NICU of some general hospital, we couldn't stretch the result in our favor. Therefore, continuous studies are suggested.

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방사선 조사가 백서 하악과두 연골 형성에 미치는 영향에 관한 형태계측학적 연구 (Morphometric Study of the Irradiation Effect on the Cartilage Formation in the Rat Mandibular Condyle)

  • 김정화;허민석;이삼선;최순철
    • 치과방사선
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    • 제29권1호
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    • pp.87-103
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    • 1999
  • Purpose: This study was undertaken to quantitatively estimate the degree of the damage and recovery of the irradiated rat condylar cartilage using the Image Analyzer. Materials and Methods: Experimental animals were 16 male rats of the Sprague-Dawley strain at the age of 20 day irradiated with the dose of 10 Gy in their head and neck region. Four rats were sacrificed at the each of the following time intervals - 1, 4, 7 and 14 days, respectively. The same number of control group animals were sacrificed at the each age of 21. 24, 27 and 34 days, respectively. The specimens were stained with 0.5% toluidine blue and examined with light microscope. The condylar cartilage was divided into 4 zones; fibrous zone, proliferating zone, upper hypertrophic zone, and lower hypertrophic zone. And then, the proliferating zone was subdivided into 2 layers - upper and lower layer, and upper and lower hypertrophic zone were subdivided into three layers, respectively - upper, middle and lower layer. With the aid of Image Analyzer, morphometric analysis was performed. The thickness, the numerical density of cells, the cell area density, the extracellular matrix area density, the mean area of single cell, the mean area of extracellular matrix per single cell were measured and analysed. Results: In the experimental group, the thickness of the fibrous zone was slightly increased and that of the proliferating zone and the upper and the lower hypertrophic zone was markedly decreased. With time, the thickness of the fibrous zone was gradually increased and that of the proliferating zone and the upper and the lower hypertrophic zone was steadily in the decreased state. The numerical density of cells of the proliferating zone was increased on post-irradiated 1 day, but decreased after post-irradiated 4 day, and that of the upper hypertrophic zone was decreased. The numerical density of cells of the lower hypertrophic zone was decreased in the early stage and then was decreased or not significantly different from that of the control group with time. In the experimental group, the cell area density of the fibrous zone and the proliferating zone was decreased in the early stage and then gradually increased or not significantly different from that of the control group with time. The cell area density of the upper and the lower hypertrophic zone was varied with time. The extracellular matrix area density value were totally opposite to the cell area density values: The mean area of single cell of the fibrous zone and the proliferating zone was .decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of single cell of the upper hypertrophic zone was varied with each layer and time. In the experimental group, the mean area of extracellular matrix per single cell of the fibrous zone was not significantly different with control group, and that of the proliferating zone was decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of extracellular matrix per single cell of the lower hypertrophic zone was increased in the early stage. and that of upper hypertrophic zone was varied with each layer and time. Conclusion: The condylar cartilages of rats were affected by irradiation, but the changes were vaned with each layer and time. By morphometric analysis. the changes of the cells of the condylar cartilage of irradiated rat could be calculated quantitatively.

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