• 대한바이러스학회지
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• 제27권1호
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• pp.29-37
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• 1997

To use recombinant viruses with wider host range as viral insecticides, we investigated the characteristics and pathogenicity of host range expanded recombinant viruses in insect cells. We compared host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori NPV (BmNPV), to host range expanded AcNPV, Ac-BH, by substitution of the 0.6 Kb fragment of the BmNPV helicase gene. Restriction endonuclease profiles of RecS-B6 and RecB-8 DNAs were different from those of parent viruses. Nucleotide sequence analysis of the 0.6 Kb region in the putative helicase gene of RecS-B6 and RecB-8 showed that their structures were identical to the counterpart region of BmNPV. Comparison of viral replication of these recombinant viruses in Sf-21 and BmN-4 cells showed that Ac-BH, compared to wild type viruses, replicated well in BmN-4 cells but poorly in Sf-21 cells. In contrast, RecS-B6 and RecB-8 replicated relatively well in both cells compared to parent viruses. These results may imply that random genomic recombinant viruses, RecS-B6 and RecB-8, possess better potential as viral pesticides than helicase-mediated recombinant virus, Ac-BH.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1997년도 추계 학술대회지
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• pp.66-67
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• 1997

• 한국유기농업학회지
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• 제13권1호
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• pp.23-51
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• 2005

In this article the author provided a brief introduction to the function and activation of 4-H fanning organization. The strategies developed to improve the environmental-friendly Agriculture are as following : the efficiency cooperatives of 4-H fanning organization, invitation of the upbringing plan of organization leader for promotion of the pilot region project of environmental-friendly Agriculture connected with green tourism, the establishment of reasonable management plan and strategies. In conclusion, there must be strategic measures to increase the income of environmental-friendly farmers. All of this information could be useful for the further development of the strategy for environmental-friendly Agriculture in organization and management.

• 한국유기농업학회지
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• 제17권2호
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• pp.151-170
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• 2009

The objectives of this study were to analysis the nutrient cycles of agricultural by-products. The region, in which agricultural by-products are circulated for use within the agroecosystem for minimizing the input of artificial nutrients as well as generation of wastes, and where ecologically industrial diversity is formed, was defined as the regional agriculture focused on eco-industrial approach. Plan in stages for promoting ahead with the regional agriculture focused on eco-industrial approach is as follows: First of all, "The Consultation Body for Circulating & Using By-products" should be formed. Secondly, usage of agricultural by-products is identified to select the facilities of recycling and its location including the type and amount. Thirdly, the facilities for recycling of resources and infrastructure are installed. Finally, the facilities for recycling and resource circulation system within the area are efficiently managed.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.142-143
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• 2003

Ammi majus (white lace flower, Unbelliferae) is an ornamental plant used for cut-flower arrangements worldwide. A potyvirus was isolated from its leaves with mosaic and chlorotic symptoms in the cultivated field of Chiba, Japan. Compared with Japanese homuort mosaic virus (JHMV) previously isolated from Cryptotaenia japonica, it showed similar characteristics in host reactions and molecular properties. The nucleotide sequences of coat protein and 3'- nontranslated region were highly homologous and shared 87％ and 91％ identities with those of JHMV, respectively. This virus was thus supposed to be an isolate of JHMV and designated as JHMV-Am. Phylogenetic tree was constructed using CP nucleotide sequences of the two isolates and other potyviruses previously reported. JHMV-Am and JHMV fell into a cluster with Korean strain of Zantedeschia mosaic virus (ZaMV-KR). However, low identity in amino acid sequences was found in the termini of CP genes between the two isolates of JHMV and ZaMV-KR.

• 한국유기농업학회지
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• 제27권3호
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• pp.307-325
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• 2019

This study aims to analyze the regional variations in which appear during the formation process of educational community in rural villages from the perspective of regional innovation theory. The study results revealed that the activities to form an educational community of the Songak village significantly influenced the variations across the social, economic and environmental aspects beyond an innovation of public education within the region based on interrelation between schools and villages. In other words, the activities including interactions and group learnings have been actively promoted among the various relevant stakeholders, thereby resulting in a variety of positive variations such as expanded regional welfare base, activated voluntary residents community gatherings based on the unity and cooperation, broadened socioeconomic opportunities, satisfaction on educational and cultural desires, and construction of healthy ecological environment in the region. Therefore, it is thought that regional development policy suitable for each region needs to be established by embracing the fact that activities to form a village educational community entail significant implication and value as the comprehensive regional innovation activities across the entire domains in each region.

• 대한수의학회지
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• 제28권2호
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• pp.251-259
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• 1988

Regional distribution and relative frequency of endocrine cells in ten portions of the gastrointestinal tract of the Korean native cattle were observed by immunocytochemical methods using specific antisera against chromogranin, serotonin, somatostatin, glucagon, bovine pancreatic polypeptide(BPP), motilin, gastric inhibitory polypeptide(GIP), neurotensin, secretin, gastrin and substance P. The results observed are summarized as follows: In the abomasum, chromogranin-, serotonin-, somatostatin-, motilin-, glucagon-, gastrin-, and substance P-immunoreactive cells were found. Chromogranin-and serotonin-immunoreactive cells were more numerous in the fundic region than pyloric region. Somatostatin- and gastrinimmunoreactive cells were numerous in the pyloric region than in the fundic region. In the small intestine, chromogranin-, serotonin-, somatostatin-, glucagon-, BPP-, motilin-, gastrin-, GIP-, neurotensin-, secretin-, and substance P-immunoreactive cells were detected. Chromogranin-, somatostatin-, GIP- and secretin-immunoreactive cells were most numerous in the duodenum, while BPP-, motilin-, glucagon-, neurotensin- and substance P-immunoreactive cells were rarely seen in the small intestine. In the large intestine, chromogranin-, serotonin- and BPP-immunoreactive cells were widely distributed and most numerous in the rectum. Somatostatin-, glucagon- and substance P-immunoreactive cells were rarely seen in the large intestine.

• Asian-Australasian Journal of Animal Sciences
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• 제24권5호
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• pp.610-615
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• 2011

We examined the correlations between lactation curve shape, including persistency and changes in body condition score (BCS) during early-stage (0 to 30 days in milk (DIM)), nadir-stage (31 to 90 DIM), and late-stage (91 to 240 DIM) lactation in 191 first-lactation cows. Data used were first-parity BCS records, scored twice every month after calving, and daily milk yields. Individual lactation curves were depicted by the Wilmink function. Lactation persistency was defined as the difference in estimated milk yields between 240 DIM and 60 DIM. Changes in BCSs in the early and late stages were defined as linear regression coefficients. There were no significant correlations between traits for lactation curve shape and change in BCS in early-stage lactation. Peak yield and total milk yield were negatively correlated with BCSs in nadir- and late-stage lactation and with BCS change in late-stage lactation, suggesting that cows with high lactation yields had low body reserves and health status in mid- to late lactation and had delayed recovery of body reserves. Lactation persistency was positively correlated with change in BCS in late-stage lactation, suggesting that cows with high lactation persistency tended to be healthy and to recover their body reserves well in late lactation.

• Reproductive and Developmental Biology
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• 제41권3호
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• pp.51-55
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• 2017

The production of therapeutic protein from transgenic domestic animal is the major technology of biotechnology. Insulin-like growth factor-1 (IGF-1) is known to play an important role in the growth of the animal. The objective of this study is construction of knock-in vector that bovine IGF-1 gene is inserted into the exon 7 locus of ${\beta}$-casein gene and expressed using the gene regulatory DNA sequence of bovine ${\beta}$-casein gene. The knock-in vector consists of 5' arm region (1.02 kb), bIGF-1 cDNA, CMV-EGFP, and 3' arm region (1.81 kb). To express bIGF-1 gene as transgene, the F2A sequence was fused to the 5' terminal of bIGF-1 gene and inserted into exon 7 of the ${\beta}$-casein gene. As a result, the knock-in vector is confirmed that the amino acids are synthesized without termination from the ${\beta}$-casein exon 7 region to the bIGF-1 gene by DNA sequence. These knock-in vectors may help to create transgenic dairy cattle expressing bovine bIGF-1 protein in the mammary gland via the expression system of the bovine ${\beta}$-casein gene.

• 한국자원식물학회지
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• 제21권1호
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• pp.41-46
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• 2008

차나무의 분자학적 유연관계 및 유전적 다양성을 알아보기 위하여 한국에서 자생하는 차나무 집단 21개 지역 차나무에 대하여 DFR 유전자 부위를 이용하여 PCR-RFLP분석을 하였다. DFR 4+5 primer 쌍을 이용하여 증폭결과 DFR유전자의 크기는 약 1.4kb에서 PCR산물을 획득하였다. PCR산물에 제한효소 Hpa II 및 Mse I를 이용하여 RFLP분석 결과 차나무 집단간 또는 집단내 차나무간의 유전적 다양성을 보였다. Hpa II 제한효소를 이용한 RFLP 밴드패턴은 3가지 형태로 구분되었으며, 같은 차나무 집단내에서도 유전적 다양성이 나타났다. Mse I 제한효소를 이용한 밴드패턴은 6가지의 형태로 다양성을 보였으며, 웅포집단 차나무의 경우 집단 내 다양성이 2가지 형태로 나타나 같은 집단내에서의 유전적 변이는 적은 것으로 보인다. 본 연구에서 사용한 2가지의 제한효소의 결과 차나무의 집단간 또는 같은 집단내의 차나무간에서 유전적 다양성을 확인할 수 있었다.