The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Characteristics and Pathogenicity of Host Range Expanded Recombinant Viruses in Insect Cells

숙주범위가 넓어진 재조합 바이러스의 세포주에서의 특성 및 병원성

  • Kim, Hye-Sung (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Woo, Soo-Dong (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kim, Woo-Jin (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi, Jae-Young (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Jin, Byung-Rae (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Lee, Youn-Hyung (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kang, Seok-Kwon (Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Seoul National University)
  • 김혜성 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 우수동 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 김우진 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 최재영 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 진병래 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 이윤형 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 강석권 (서울대학교 농업생명과학대학 응용생물화학부)
  • Published : 1997.06.30
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Abstract

To use recombinant viruses with wider host range as viral insecticides, we investigated the characteristics and pathogenicity of host range expanded recombinant viruses in insect cells. We compared host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori NPV (BmNPV), to host range expanded AcNPV, Ac-BH, by substitution of the 0.6 Kb fragment of the BmNPV helicase gene. Restriction endonuclease profiles of RecS-B6 and RecB-8 DNAs were different from those of parent viruses. Nucleotide sequence analysis of the 0.6 Kb region in the putative helicase gene of RecS-B6 and RecB-8 showed that their structures were identical to the counterpart region of BmNPV. Comparison of viral replication of these recombinant viruses in Sf-21 and BmN-4 cells showed that Ac-BH, compared to wild type viruses, replicated well in BmN-4 cells but poorly in Sf-21 cells. In contrast, RecS-B6 and RecB-8 replicated relatively well in both cells compared to parent viruses. These results may imply that random genomic recombinant viruses, RecS-B6 and RecB-8, possess better potential as viral pesticides than helicase-mediated recombinant virus, Ac-BH.

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