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http://dx.doi.org/10.12749/RDB.2017.41.3.51

Knock-in Vector for Expression of Insulin-like Growth Factor 1 on the Bovine β-casein Gene Locus  

Kim, Sang Young (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
Park, Da Som (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
Kim, Se Eun (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
Kang, Man-Jong (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
Publication Information
Reproductive and Developmental Biology / v.41, no.3, 2017 , pp. 51-55 More about this Journal
Abstract
The production of therapeutic protein from transgenic domestic animal is the major technology of biotechnology. Insulin-like growth factor-1 (IGF-1) is known to play an important role in the growth of the animal. The objective of this study is construction of knock-in vector that bovine IGF-1 gene is inserted into the exon 7 locus of ${\beta}$-casein gene and expressed using the gene regulatory DNA sequence of bovine ${\beta}$-casein gene. The knock-in vector consists of 5' arm region (1.02 kb), bIGF-1 cDNA, CMV-EGFP, and 3' arm region (1.81 kb). To express bIGF-1 gene as transgene, the F2A sequence was fused to the 5' terminal of bIGF-1 gene and inserted into exon 7 of the ${\beta}$-casein gene. As a result, the knock-in vector is confirmed that the amino acids are synthesized without termination from the ${\beta}$-casein exon 7 region to the bIGF-1 gene by DNA sequence. These knock-in vectors may help to create transgenic dairy cattle expressing bovine bIGF-1 protein in the mammary gland via the expression system of the bovine ${\beta}$-casein gene.
Keywords
Knock-in; Insulin-like growth factor 1; ${\beta}$-casein gene locus;
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Times Cited By KSCI : 1  (Citation Analysis)
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