• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.89-98
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• 2019

The aim of this study was to evaluate the change of phenolic compounds after bordeaux mixture treatment on Cheorwon onion. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is cultivated much in the temperate regions of the world. Onion is a good name for the 'Okchong' to drop blood cholesterol and cardiovascular blood flow to increase the prevention of adult diseases. Cheorwon area is inland, but it has high continental climate due to its high altitude. After the treatment with bordeaux mixture, the weight, width and plant height of the onion were affected to some extent. In the phenolic compounds, caffeic acid, ferulic acid, rutin, and kaempferol except benzoic acid, p-coumaric acid and quercetin were similar to or less than the control. Therefore, it was concluded that the treatment with bordeaux mixture had some effect on onion growth and phenolic compound contents.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.27-33
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• 2019

The purpose of this study was to compare the storage and post-storage changes of Cheorwon onions and onions for each region. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is cultivated much in the temperate regions of the world. Cheorwon area is inland, but it has high continental climate due to its high altitude. Therefore it is said that the onion cultivated in this region has higher sugar content and higher taste than onion grown in southern region. Although the results of onion storage experiments in Cheorwon, Muan, Buan, and Changnyeong regions did not show much difference in weight, width, and height measurements before and after storage, Brix was reduced by approximately 1-2 brix. Before and after storing in each region, Brix was the highest in Cheorwon, Changnyeong, Buan, and Muan, and after saving, onions in Cheorwon were the same order. After each region was stored, the results showed that Cheorwon (112.3g), Muan (106g), Buan (102.1g) and Changnyeong (92.6g) had the highest hardness. Cold storage tests show that onions contain the least amount of corruption in paper boxes, while Cheorwon onions have the lowest level of corruption in other areas.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.105-114
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• 2018

The aim of this study was to evaluate the change of phenolic compounds after phosphite treatment on Cheorwon onion. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is widely cultivated in the temperate regions of the world. Onion is a good name for the 'Okchong' to drop blood cholesterol and cardiovascular blood flow to increase the prevention of adult diseases. Cheorwon area is inland, but it has high continental climate due to its high altitude. Therefore it is said that the onion cultivated in this region has higher sugar content and higher taste than onion grown in the southern region. Phosphorus components are particularly important ingredients for promoting muscle development. However, if the phosphoric acid content of the soil part is maintained to a large extent until the harvest, the competition of the nutrients tends to cause decay of the root part. Therefore, it is important to improve the quality and shelf life of onion by inducing nutrient balance by applying foliar fertilization method on the reducing phosphorus at harvest time. In this study, acidity was controlled by diluting phosphorous acid(H₃PO₃) and potassium hydroxide(KOH), followed by leaf surface treatment with phosphite on onion. In this study, the concentration of phosphite was diluted to 500, 1,000, 1,500ppm and sprayed three times over the onion leaves in May 2018 using an atomizer and harvested at the end of June, and the phenolic compounds were analyzed by HPLC. As a result, the content of quercetin, one of the important substances in onion, was phosphite 500ppm(179.70㎍/g), 1,000(150.27), 1,500(105.95). The contents of caffeic acid, p-coumaric acid, ferulic acid, rutin, kaempferol, and sugar content were higher in the treatments than in the control. Therefore, the phosphite does not have a great influence on the growth, but it may play a role as a method of achieving balance with nitrogen in the rainy season by supplying the role of the material catalyst and the water soluble phosphoric acid and the potassium in the influence of the material change.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.103-114
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• 2019

In this study we examined the preferences of college life factors for students at Korea National College of Agriculture and Fisheries(KNCAF). Analytical techniques of unstructured data used opinion mining and text mining techniques, and the results of text mining were visualized as word cloud. And those results were used for statistical analysis of the students' willingness to farm after graduation. The items of the favorable survey consisted of 10 items in 5 areas including university image, self-capacity, dormitory, education system, and future vision. After classifying the emotions of positive and negative in the collected questionnaire, a dictionary of positive and negative was created to evaluate the preference. The items of 'college image' at the time of university support, 'self after 10 years' after graduation, 'self-capacity' and 'present KNCAF' showed high positive emotion. On the other hand, positive emotion was low in the items of 'college dormitory', 'educational course', 'long-term field practice' and 'future of Korean agriculture'. In the cross-analysis of the difference in the will to farming according to gender, farming base, and entrance motivation, the will to farm according to gender and entrance motivation showed statistically significant results, but it was not significant in farming base. Also in binary logistic regression analysis on the will to farming, the statistically significant variable was found to be 'motivation for admission'

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.102-102
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• 2022

More than 74% of forage crops in Korea are cultivated in winter rice fields. Italian ryegrass (IRG) can be said to be a representative forage crop, but over 70% of its seeds are dependent on imports. In addition, there are concerns that the stable supply of research fees may be disrupted due to the effects of climate change, war, and other factors. Therefore, it is necessary to secure a large production area for stable supply. Korea's reclaimed land area is 135,000 ha and its agricultural area is 112,000 ha. Therefore, this study attempted to apply IRG to the domestic IRG seed industry through stable cultivation techniques on reclaimed land. In this study, IRG 'Kowiearly' varsity was cultivated in late October 2020 and early October 2021 in the Saemangeum reclaimed land. The seeding methods were conventional drill sowing seeding, new technology spot seedling (30 × 18 cm) and new technology drill sowing seeding. The amount of sowing was conventional drill sowing seeding 2.0(kg/10a), new technology spot seedling 1.5(kg/10a), and new technology drill sowing seeding 1.5(kg/10a). Fertilizer application amount is conventional drill sowing seeding(N-P₂O₅-K₂O) 9.0-12-12(kg/10a), and new technology spot seedling and drill sowing seeding were(N-P₂O₅-K₂O) 4.5-12-12(kg/10a) respectively. Fertilizer was applied accordingly. After that, in February, the conventional drill sowing seeding, new technology drill sowing seeding and spot seedling applied 4.0 and 2.2(kg/10a) of nitrogen supplement fertilizer, respectively. Before wintering, plant length was higher in 2022 than in 2021, but leaf number was higher in 2021. Heading time was April 30, 2021 and April 25, 2022. In heading time, plant length was 74 cm in 2021 and 67 cm in 2022, lower than in 2021. On the other hand, There was no difference in the number of panicle and the number of seeds in the 2021 harvester in all treatment plots, and, thus seed yield was no differ. However, the drill sowing seeding and spot seedling of the new technology were somewhat higher than the conventional drill sowing seeding. On the other hand, seed yield was decreased in all treatment plots compared to 2022 because of raifall deficiency in 2021.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.111-111
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• 2022

According to data from the Ministry of Environment, food waste accounted for 27% of the nation5 s household waste in 2020, and 4.67 million tons of food waste is being discharged per year. According to the Food Waste Direct Landfill Prohibition Act, food waste must be incinerated, composted, fodder, and decomposed before landfilling. The cost of incineration and landfilling of food waste is considerable. Therefore, through the process of turning food waste into fertilizer, we are going to investigate the limit of crop application and the change in the growth of crops during continuous use of food waste fertilizer. This study investigated the growth of lettuce such as shoot length, root length, leaf number, fresh weight, and dry weight after treating lettuce with food waste dry powder mixed fertilizer. The experiment was carried out continuously in 2021 (1st year) and 2022 (2nd year), and the treatment groups were set to No Treatment (NT), Chemical Fertilizer (CF), Mixed Fertilizer (MF×1), and Mixed Fertilizer×2 (MF×2), was repeated 3 times. As a result of the 1st year growth survey, there was no significant difference between NT and CF in the case of shoot length, but MF×1 and MF×2 were significantly decreased compared to NT. Root length was not significantly different in all treatment groups. As for the leaf number, there was no significant difference between NT and MF×1, but CF and MF×2 were significantly decreased compared to NT. In fresh weight, MF×1 and MF×2 were significantly decreased compared to NT, and in the case of dry weight, there was no significant difference between NT, MF×1, and MF×2. As a result of the 2nd year growth survey, there was a significant difference in CF and MF×2 in leaf number, but there was no significant difference in all treatment groups with respect to shoot length, root length, fresh weight, and dry weight. Through continuous additional research, it is necessary to confirm the change in soil composition and the growth of crops due to food waste fertilizer treatment.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.114-114
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• 2022

There are 28 families and 166 species of exotic weeds on agricultural land and among these, 23 families and 80 species of exotic weeds occur on pastures. Among them, the Solidago altissima is a perennial weed belonging to the asteraceae family and it is an exotic weed that spreads to the surrounding area using methods such as high seed production, vegetative propagation using underground rhizomes and allelochemical. Accordingly, in 2009, the Ministry of Environment designated it as an ecosystem-disrupting species. This study was conducted to obtain basic data about the effects of S.altissima derived allelochemicals on forage crops. The root of S.altissima was separated, dried in the shade and then pulverized to prepare an root powder. Powder was repeatedly extracted with methanol for 3 days and concentrated under reduced pressure to obtain an root methanol extract. Dissolve the extract in distilled water, dispense it in a separate-funnel and proceed with liquid-liquid extraction by adding equal amounts of n-haxane (Hex), chloroform (CHCI₃), ethyl acetate (EtoAC), and butanol (BuOH) in order of increasing polarity. A seed-bioassay was performed using fractions for each solvent, followed by separation and purification by silica gel column chromatography. As a result of the fraction germination test for each solvent, the IC₅₀ values using the fresh weight of each fraction were 898.3 mg L^-1, 676.3 mg L^-1, 1160 mg L^-1 and 1360 mg L^-1. CA, CB, and CC fractions were obtained through primary silica gel column chromatography that used CHCI₃ fraction. As a result of seed-bioassay using each fraction, the IC₅₀ values for the fresh weight of each fraction was 537.3 mg L^-1, 1280 mg L^-1 and 1947 mg L^-1. Based on this, 5 fractions were obtained as a result of secondary silica gel column chromatography using the CA fraction. A seed-bioassay was performed, as a result, the lowest IC₅₀ value was calculated as 226.7 mg L^-1 in the CAE fraction. Based on this, the fraction was analyzed by GC-MS. The results of this study can be used as basic research data on the effects of weeds on forage crops and allelochemicals secreted from S. altissima.

• Journal of Veterinary Science
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• v.24 no.5
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• pp.73.1-73.16
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• 2023

Background: Highly pathogenic avian influenza virus (HPAIV) is considered a global threat to both human health and the poultry industry. MicroRNAs (miRNA) can modulate the immune system by affecting gene expression patterns in HPAIV-infected chickens. Objectives: To gain further insights into the role of miRNAs in immune responses against H5N1 infection, as well as the development of strategies for breeding disease-resistant chickens, we characterized miRNA expression patterns in tracheal tissues from H5N1-infected Ri chickens. Methods: miRNAs expression was analyzed from two H5N1-infected Ri chicken lines using small RNA sequencing. The target genes of differentially expressed (DE) miRNAs were predicted using miRDB. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis were then conducted. Furthermore, using quantitative real-time polymerase chain reaction, we validated the expression levels of DE miRNAs (miR-22-3p, miR-146b-3p, miR27b-3p, miR-128-3p, miR-2188-5p, miR-451, miR-205a, miR-203a, miR-21-3p, and miR-200a3p) from all comparisons and their immune-related target genes. Results: A total of 53 miRNAs were significantly expressed in the infection samples of the resistant compared to the susceptible line. Network analyses between the DE miRNAs and target genes revealed that DE miRNAs may regulate the expression of target genes involved in the transforming growth factor-beta, mitogen-activated protein kinase, and Toll-like receptor signaling pathways, all of which are related to influenza A virus progression. Conclusions: Collectively, our results provided novel insights into the miRNA expression patterns of tracheal tissues from H5N1-infected Ri chickens. More importantly, our findings offer insights into the relationship between miRNA and immune-related target genes and the role of miRNA in HPAIV infections in chickens.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.720-734
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• 2023

In Korea, Korea Proven Bulls (KPN) program has been well-developed. Breeding and evaluation of cows are also an essential factor to increase earnings and genetic gain. This study aimed to evaluate the accuracy of cow breeding value by using three methods (pedigree index [PI], pedigree-based best linear unbiased prediction [PBLUP], and genomic-BLUP [GBLUP]). The reference population (n = 16,971) was used to estimate breeding values for 481 females as a test population. The accuracy of GBLUP was 0.63, 0.66, 0.62 and 0.63 for carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS), respectively. As for the PBLUP method, accuracy of prediction was 0.43 for CWT, 0.45 for EMA, 0.43 for MS, and 0.44 for BFT. Accuracy of PI method was the lowest (0.28 to 0.29 for carcass traits). The increase by approximate 20% in accuracy of GBLUP method than other methods could be because genomic information may explain Mendelian sampling error that pedigree information cannot detect. Bias can cause reducing accuracy of estimated breeding value (EBV) for selected animals. Regression coefficient between true breeding value (TBV) and GBLUP EBV, PBLUP EBV, and PI EBV were 0.78, 0.625, and 0.35, respectively for CWT. This showed that genomic EBV (GEBV) is less biased than PBLUP and PI EBV in this study. In addition, number of effective chromosome segments (M_e) statistic that indicates the independent loci is one of the important factors affecting the accuracy of BLUP. The correlation between M_e and the accuracy of GBLUP is related to the genetic relationship between reference and test population. The correlations between M_e and accuracy were -0.74 in CWT, -0.75 in EMA, -0.73 in MS, and -0.75 in BF, which were strongly negative. These results proved that the estimation of genetic ability using genomic data is the most effective, and the smaller the M_e, the higher the accuracy of EBV.

• Economic and Environmental Geology
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• v.56 no.5
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• pp.619-628
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• 2023

In this study, we investigated the feasibility of calcium sulfate fertilizer as a stabilizing agent for As and Hg contaminated farmland soil and its stabilization characteristics in 3 different physical forms (particulate, powder, and solution) through a pot experiment including 34 days of lettuce growth. As and Hg contents of the lettuce grown in the stabilized soils were decreased by at least 70%. However the lettuce yield of the soil stabilized with the solution agent was decreased by 46% due to the overabundance of the nutrients from the solution agent. Thus, if a solution-type agent is planned for agricultural farmland soil stabilization, additional tests for optimal dosage are needed to preserve vegetation growth. In Hg fractionation, a lower concentration of elemental fractions and a higher concentration of residual/sulfide fractions were identified in the soils stabilized with the solution, powder, and pariculate agents in descending order while there were no significant changes in As fractionation. Overall results suggest that calcium sulfate fertilizer can be used as a stabilizing agent, and a solution-type agent could be used when the operation of heavy machinery for the soil stabilization process is impossible.