• Journal of Plant Biotechnology
• /
• 제48권2호
• /
• pp.100-105
• /
• 2021

Genetic engineering is a potential approach to improve secondary metabolism in plants. In order to elucidate the effect of production of anthocyanin pigment 1 (PAP1) overexpression on the bioactivity of ginseng, we analyzed its antioxidant, antimicrobial, and anti-elastase activities in this study. Our results showed that PAP1 overexpression increased the production of polyphenolic compounds including anthocyanins. The antioxidant, antimicrobial, and anti-elastase activities were stronger in anthocyanin-overproducing ginseng hairy roots (AOX) than in wild ginseng hairy roots. Using a different solvent system (0, 30, 70, and 100% (v/v) EtOH), we revealed that variations in the contents of the polyphenolic compounds were highly correlated with changes in the antioxidant and antimicrobial activities of AOX. The antioxidant, antimicrobial, and anti-elastase effects of AOX highlight genetic engineering as a powerful approach to enhance the therapeutic properties of plants. Our results show that AOX could potentially have various functional applications in the cosmetic and pharmaceutical industries.

• 농촌지도와개발
• /
• 제4권1호
• /
• pp.175-193
• /
• 1997

The agricultural development of Korea has occurred at the expense of natural resources and environmental quality, and, today, one of the most urgent problem in the practices of intensive livestock farming is disposing of the animal wastewater. As a result, soil and water degradation and other environmental deterioration become apparent and they cause long-term loss in agricultural production. This paper attempted to summarize the environmental problems associated with agricultural activities, and to get some implications to minimize agricultural environment problems. It is proposed that sustainable agricultural is one of desirable directions for future Korean agriculture. The goal of the sustainable agricultural effort is to utilize the potential environmental quality problem. Agricultural operations may be more dependent upon the production practices and waste management techniques utilized by farmers than the size of the operation, the number of animal fed, or amount of waste involved. Also low-input farming technology can be suggested one of them, and disposing system of agricultural residues and animal waste should be developed with intensive concerns and financial supports.

• 농촌계획
• /
• 제23권1호
• /
• pp.135-146
• /
• 2017

The objective of this study is to accurately understand the spatial distribution characteristics of the main production area for the three vegetable crops such as Chinese cabbage, radish, and hot pepper. We applied the 8 landscape indices such as TA, NP, PD, LPI, LSI, PLADJ, COHESION, and CONNECT to 35 cities and counties using FRAGSTATS. In the case of main production area for Chinese cabbage, six cities and counties in Gangwon province were revealed as a relatively high degree of aggregation by cultivation parcels than other area. In addition, Gangneung city and Hongcheon county have been analyzed to be the most aggregated area in the case of radish and hot pepper, respectively. In the future, the spatial analysis method used in this study would be helpful to develop an effective regional plan of the main production area.

• 한국환경농학회지
• /
• 제27권2호
• /
• pp.145-149
• /
• 2008

Anaerobic co-digestion of swine manure and food waste for biogas production was performed in serum bottles at various volatile solids(VS) contents and mixing ratios of two substrates(swine manure:food waste=$100:0{\sim}0:100$). Through kinetic mode of surface methodology, the methane production was fitted to a Gompertz equation. The ultimate methane production potential of swine manure alone was lower than that of food waste regardless of VS contents. However, it was appeared that maximum methane production potentials in 80 : 20 of the mixing rate at VS 3% was enhanced at 144.7%, compared to its only swine manure. The potential increased up to 815.71 ml/g VS fed as VS concentration and food composition increased up to 3.0% and 20%, respectively. The ultimate amount of methane produced had significantly a positive relationship with that of methane yield rate. Overall, it would be strongly recommended that feeding stocks use 20% of mixing ratio of food waste based on VS 3% contents when operating the anaerobic reactor on site at $35^{\circ}C$ if not have treatment of its anaerobic waste water.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권3호
• /
• pp.400-406
• /
• 2011

An experiment was carried out to assess the effects of xylanase supplementation on the performance, net energy and gut microflora of broilers fed a wheat-based diet. Day-old male broiler chicks were allocated to two dietary treatments. Each treatment was composed of six replicate cages of seven broilers per cage. The diets were wheat-based and offered as mash. The treatments included i) basal diet deficient in metabolizable energy; and ii) basal diet supplemented with a commercial xylanase added at 4,000 U/kg feed. Bird performance, nutrient utilization and gut microbial populations were measured. Heat production and net energy were determined using an auto-control, open circuit respiration calorimetry apparatus. Results showed that exogenous xylanase supplementation improved feed conversion efficiency (p<0.05) and increased diet AME (+4.2%; p<0.05), as well as heat production (HP), net energy for production (NEp), production of $CO_2$, and consumption of $O_2$. The respiratory quotient (RQ) was also increased (p<0.01) by the addition of xylanase. NEp value was increased by 26.1% while daily heat production per kg metabolizable body weight was decreased by 26.2% when the xylanase was added. Xylanase supplementation numerically increased the ileal digestibility of protein and energy by 3 and 6 percentage units respectively (p>0.05). The ileal digestibility of hemicellulose was significantly improved by xylanase addition (p<0.05).

• Animal Bioscience
• /
• 제36권6호
• /
• pp.973-979
• /
• 2023

Objective: The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, which is the most efficient and reliable tool for precisely targeted modification of the genome of living cells, has generated considerable excitement for industrial applications as well as scientific research. In this study, we developed a gene-editing and detection system for chick embryo sexing during the embryonic stage. Methods: By combining the CRISPR/Cas9 technical platform and germ cell-mediated germline transmission, we not only generated Z chromosome-targeted knockin chickens but also developed a detection system for fluorescence-positive male chicks in the embryonic stage. Results: We targeted a green fluorescent protein (GFP) transgene into a specific locus on the Z chromosome of chicken primordial germ cells (PGCs), resulting in the production of Z^GFP-knockin chickens. By mating Z^GFP-knockin females (Z^GFP/W) with wild males (Z/Z) and using a GFP detection system, we could identify chick sex, as the GFP transgene was expressed on the Z chromosome only in male offspring (Z^GFP/Z) even before hatching. Conclusion: Our results demonstrate that the CRISPR/Cas9 technical platform with chicken PGCs facilitates the production of specific genome-edited chickens for basic research as well as practical applications.

• 한국균학회지
• /
• 제38권2호
• /
• pp.199-201
• /
• 2010

Ganoderma neo-japonicum, which is also known as black lingshi mushroom and medicinal mushroom. Present experiments were conducted to determine the possibility of artificial culture with oak sawdust of G. neo-japonicum. The duration of mycelial growth and days of pinhead formation of oak sawdust bag (2.4 kg) were 28~35 days and 25~29 days, respectively. The yield of mushroom fresh fruitbody was 135~157 g.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제9권1호
• /
• pp.95-99
• /
• 2004

This study was carried out to investigate the effect of body weight of queen on the developmental characteristics of foundation queens in the Bombus ignitus collected in Korean locality. Queens were classified into six groups based on their body weight (i.e., 0.4 g, 0.5 g, 0.6 g, 0.7 g, 0.8 g and 0.9 g). The average weight of 200 foundation queens collected was 0.657${\times}40.095 g and 0.6 g-class was most abundant (38 %) among six weight classes. The queen that had the heavy body weight showed the trends of higher rate of oviposition, colony foundation and progeny-queen production and shorter periods of preoviposition and colony foundation, suggesting the positive correlation between the body weight of queen and colony developmental characteristics. Also, the numbers of worker and progeny-queen emerged from the queen with heavy body weight were slightly higher than those with light body weight queen.

• Journal of Microbiology and Biotechnology
• /
• 제13권5호
• /
• pp.815-818
• /
• 2003

Maltosyl(G2)-erythritol, produced by the transglycosylation reaction of erythritol with maltotriose by Bacillus stearothermophilus maltogenic amylase, was not utilized either as a substrate for lactic acid production or for water-insoluble glucan synthesis. An inhibition assay of dextransucrase and mutansucrase showed that the dental caries suppression effect of G2-erythritol was greater than that of erythritol.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제11권1호
• /
• pp.43-48
• /
• 2005

We investigated possible effect of different concentration of sugar solution and addition of antiseptic in the solution on oviposition and colony development of Bombus ignitus and B. terrestris. The rates of oviposition, colony foundation and progeny-queen production of B. ignitus were 1.2-3.0 fold higher in the 40$\%$ sugar solution than those of the 50$\%$ sugar solution. The rates of oviposition, colony foundation and progeny­queen production were 1.1-2.6 fold higher in the 40$\%$ sugar solution added in 0.3$\%$ sorbic acid as antiseptic than those of the 40$\%$ sugar solution. Further, the death rate within one month was 1.7 fold lower in the 40$\%$ sugar solution added in 0.3$\%$ sorbic acid than that of 40$\%$ sugar solution alone. In the comparison of the colony development tested using imported sugar solution, the Beehappy???, the 40$\%$ sugar solution added to antiseptic and the 40$\%$ sugar solution with­out antiseptic, the 40$\%$ sugar solution added to antiseptic was about equal to the Beehappy??? in colony development of B. terrestris. Further, the number of adults produced was 1.2-3.0 fold higher in the 40$\%$ sugar solution added to antiseptic than that of the Bee­happy???. Therefore the 40$\%$ sugar solution was more effective than the 50$\%$ sugar solution, and the 40$\%$ sugar solution added to antiseptic was the most effective in colony development and mass rearing of bumblebee.