Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Overproduction of anthocyanin in ginseng hairy roots enhances their antioxidant, antimicrobial, and anti-elastase activities

  • Jin, Sora (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Bang, Seounggun (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Ahn, Min-A (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Lee, Kyubin (School of Biological Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Kim, Kyunghwan (School of Biological Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Hyun, Tae Kyung (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University)
  • Received : 2021.05.14
  • Accepted : 2021.05.28
  • Published : 2021.06.30
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Abstract

Genetic engineering is a potential approach to improve secondary metabolism in plants. In order to elucidate the effect of production of anthocyanin pigment 1 (PAP1) overexpression on the bioactivity of ginseng, we analyzed its antioxidant, antimicrobial, and anti-elastase activities in this study. Our results showed that PAP1 overexpression increased the production of polyphenolic compounds including anthocyanins. The antioxidant, antimicrobial, and anti-elastase activities were stronger in anthocyanin-overproducing ginseng hairy roots (AOX) than in wild ginseng hairy roots. Using a different solvent system (0, 30, 70, and 100% (v/v) EtOH), we revealed that variations in the contents of the polyphenolic compounds were highly correlated with changes in the antioxidant and antimicrobial activities of AOX. The antioxidant, antimicrobial, and anti-elastase effects of AOX highlight genetic engineering as a powerful approach to enhance the therapeutic properties of plants. Our results show that AOX could potentially have various functional applications in the cosmetic and pharmaceutical industries.

Keywords

Acknowledgement

This research was supported by Chungbuk National University Korea National University Development Project (2020).

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