• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.374-381
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• 2015

This study aimed to evaluate the effects of vitamin E (VE), ferulic acid (FA) and their combination supplementation on meat quality and antioxidant capacities of finishing pigs. Sixty barrows were randomly allocated to four experimental diets using a $2{\times}2$ factorial arrangement with 2 VE supplemental levels (0 or 400 mg/kg) and 2 FA supplemental levels (0 or 100 mg/kg) in basal diets. After 28 days, six pigs per treatment were slaughtered. The results showed that VE supplementation increased loin eye area of pigs (p<0.05) and FA supplementation increased $pH_{45min}$ value (p<0.05). The interaction of $FA{\times}VE$ was observed in shear force of longissimus dorsi muscle (p<0.05). Moreover, supplementation with VE decreased hepatic and sarcous malondialdehyde (MDA) content, increased hepatic glutathione (GSH) content and sarcous glutathione peroxidase (GSH-Px) activity (p<0.05). Additionally, supplementation with FA increased hepatic GSH-Px activity and decreased sarcous MDA content (p<0.05). However, dietary treatment did not affect the expression of genes related to nuclear factor, erythroid 2-like 2 (NFE2L2) pathway. These results suggest that dietary FA and VE could partially improve meat quality and antioxidant capacity of finishing pigs, but not by activating NFE2L2 pathway under the normal conditions of farming.

• 한국기계가공학회지
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• 제19권11호
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• pp.42-48
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• 2020

The agricultural tractor is a multi-purpose vehicle, which is frequently used in the agricultural field. It must be highly reliable in terms of human safety. Design and analysis of agricultural tractors must be performed using actual agricultural workload to maintain high reliability. Additionally, the frequency with which various components and systems are used must also be taken into consideration. In this study, a tractor is built to measure its workload in the actual field. Further, the measured load was analyzed for various farming tasks. The range of ratios of consumed power to engine power was measured to be 42.6%-87.2%, 75.1%-97%, 26.5%-59.2% for a plow, rotary, and harvest tasks, respectively. The results were fed into a transmission simulation model to analyze the strength and life of the transmission components. We conclude that a more reliable product can be constructed by incorporating the transmission analyses using the actual load.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• 농약과학회지
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• 제7권4호
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• pp.258-263
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• 2003

국내 과수용 농약에 대한 농가사용실태를 파악하여 농산물의 안전성과 농약관리를 위한 자료로 활용하고자 국내 주요 과수류인 사과, 감률, 포도, 복숭아, 배, 감 등 6종의 과수를 대상으로 재배면적별 35지역 150농가를 선정하여 국내 과수재배 환경중 농약의 사용량을 조사 분석하였다. 과수용 농약의 년간 살포 회수는 배가 15.2회로 가장 많았고, 감은 7회로 가장 적었다. 단위면적당 농약사용량은 감률 39.5, 사과 22.1, 배 21.5, 복숭아 11.9, 감 10.8, 포도 6.7 a.i. kg/ha이었으며, 사과, 감률, 포도, 감의 사용량은 1999년도 사용량 다 약간 감소하였다. 주요 사용농약은 살균제는 mancozeb, propineb, dithianon 등, 살충제는 fenitrothion, chlorpyrifos, methidathion 등이었다. 조사대상 과수 6종에 사용된 농약의 총 주성분량을 계산한 결과는 2,834.7 M/T으로 2002년도 원예용 농약 출하량 대비 18.3%를 차지하였다. 또한, 국내 과수별 단위면적당 농 약사용량을 2001년도 미국의 농약사용량과 비교한 결과 사과는 22.la.i. kg/ha로 한국이 많았으나 감률 등 나머지 작물에서는 미국의 사용량이 많은 편이었다. 본 시험에서 얻어진 결과는 농약의 위해성 감소, 농업환경 보존 및 농약관리를 위해 유용한 자료로 활용될 수 있을 것으로 기대된다.

• 한국축산식품학회지
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• 제41권4호
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• pp.650-663
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• 2021

The objective of this study was to examine the relationship between meat quality attributes and the changes of sarcoplasmic protein acetylation and myofibrillar protein acetylation in lamb longissimus thoracis et lumborum muscles at different postmortem phases. Protein acetylation, color, pH, shear force, myofibril fragmentation index and cooking loss were measured. The total level of acetylated sarcoplasmic proteins showed a negative relation with pH, a positive relation with a^*, b^* and cooking loss at the pre-rigor phase. Sarcoplasmic proteins acetylation affected postmortem pH by regulating glycolysis, which in turn affects color and cooking loss. The total level of acetylated myofibrillar proteins showed a positive relation with shear force at the pre-rigor phase. Myofibrillar proteins acetylation affected meat tenderness by regulating muscle contraction. This study indicated that acetylation played a regulatory role of meat color, water-holding capacity, and tenderization process at early postmortem.

• 대한인간공학회지
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• 제30권4호
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• pp.509-515
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• 2011

Objective: The study was performed to suggest factors to consider when making or selecting protective clothing to enhance farmers' health. The purpose of this study was to review and summarize the agricultural safety technology on clothing including hat, gloves, and shoes. Background: The farmers' work clothing has to meet a multitude of requirement. Risk factors in farm-work were identified by previous researches. Agricultural safety clothing needs to be introduced. Method: We reviewed previous papers regarding the agricultural workers' health, safety clothing to protect body from excessive heat and humid environment, ultraviolet(UV) protection, and hazardous body working postures. Also the UV cut effect, pesticideproof clothing, and preventable farm work related musculoskeletal disorders were discussed. Results: The agricultural safety clothing needs to be developed to provide comfort and to be resistant against heat. The UV protection function on skin and eyes has not been fully researched in safety clothing studies yet. Conclusions and Application: The farmers' work-related body posture needs to be studied in order to make and design agricultural safety clothing. The safety issues on agricultural work shoes are also needed to be included in future studies.

• Asian-Australasian Journal of Animal Sciences
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• 제30권1호
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• pp.71-77
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• 2017

Objective: This experiment was conducted to investigate the effects of dietary supplementation with marigold extract on growth performance, pigmentation, antioxidant capacity and meat quality in broiler chickens. Methods: A total of 320 one-day-old Arbor Acres chickens were randomly divided into 5 groups with 8 replicates of 8 chickens each. The chickens of control group were fed with basal diet and other experimental groups were fed with basal diet supplemented with 0.075%, 0.15%, 0.30%, and 0.60% marigold extract respectively (the corresponding concentrations of lutein were 15, 30, 60, and 120 mg/kg). Results: The results showed that marigold extract supplementation increased the yellowness values of shank, beak, skin and muscle and the redness ($a^*$) value of thigh muscle (linear, p<0.01). Marigold extract supplementation significantly increased the total antioxidant capacity, and the activities of superoxide dismutase in liver and thigh muscle (linear, p<0.01) and significantly decreased the malondialdehyde contents of liver and thigh muscle (linear, p<0.01). Marigold extract supplementation significantly decreased the drip loss and shear force of thigh muscles (linear, p<0.01). There was no significant effect on growth performance with marigold extract supplementation. Conclusion: In conclusion, dietary supplementation of marigold extract significantly increased the yellowness values of carcass, antioxidant capacity and meat quality in broiler chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제30권4호
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• pp.569-575
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• 2017

Objective: The objective of this research was to study the electrical properties and quality of frozen-thawed chicken breast meat and to investigate the relationship between these parameters at different times of frozen storage. Methods: Thawed samples of chicken breast muscles were evaluated after being kept in frozen storage at $-18^{\circ}C$ for different periods of time (1, 2, 3, 4, 5, 6, 7, and 8 months). Results: The results showed that water-holding capacity (WHC) and protein solubility decreased while thiobarbituric acid-reactive substances content increased with increasing storage time. The impedance module of samples decreased during 8-month frozen storage. Pearson correlation coefficients showed that the impedance change ratio (Q value) was significantly (p<0.05) related to pH, color, WHC, lipid oxidation and protein solubility, indicating a good relationship between the electrical properties and qualities of frozen-thawed chicken breast meat. Conclusion: Impedance measurement has a potential to assess the quality of frozen chicken meat combining with quality indices.

• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1764-1772
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• 2017

Objective: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. Methods: Seventy-two barrow ($Duroc{\times}Landrace{\times}Yorkshire$, DLY) pigs ($65.0{\pm}2.0kg$) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected. Results: Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05). Conclusion: Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs.

• 재활복지공학회논문지
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• 제11권3호
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• pp.223-229
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• 2017

본 연구에서는 농기계 사고 대책 및 안전에 대한 연구 동향을 파악하고 그 내용을 분석하였다. 농업기계로 인한 안전을 다룬 연구는 사고 현황 조사와 사고조사 방법의 문제를 제기한 연구들로 분류할 수 있었으며, 농기계 안전사고의 원인분석에 관한 연구들은 국가차원과 포괄적인 농작업 재해 테두리 내에서 다루어지고 있었다. 안전사고의 예방과 관리에 대한 연구는 농기계 종류에 따른 사용자 요구 분석과 관리적, 교육적, 재정적 지원책에 대한 연구들이 수행되고 있었다. 또한 보다 적극적으로 농기계 사고에 대응하기 위해서 고령자의 기능과 능력에 대한 연구의 활성화가 필요함을 알 수 있었다.