Browse > Article
http://dx.doi.org/10.5713/ajas.17.0090

Effects of dietary energy sources on early postmortem muscle metabolism of finishing pigs  

Li, Yanjiao (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Yu, Changning (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Li, Jiaolong (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Zhang, Lin (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Gao, Feng (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Zhou, Guanghong (College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.12, 2017 , pp. 1764-1772 More about this Journal
Abstract
Objective: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. Methods: Seventy-two barrow ($Duroc{\times}Landrace{\times}Yorkshire$, DLY) pigs ($65.0{\pm}2.0kg$) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected. Results: Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05). Conclusion: Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs.
Keywords
Dietary Energy Source; Postmortem Metabolism; Glycolytic Enzyme; $PI3K/AKT-HIF-1{\alpha}$ Pathway; Finishing Pigs;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ferguson D, Gerrard D. Regulation of post-mortem glycolysis in ruminant muscle. Anim Prod Sci 2014;54:464-81.   DOI
2 Committee on Nutrient Requirements of Swine, National Research Council. Nutrient requirements of swine. 11th ed. Washington, DC, USA: National Academy Press; 2012.
3 Wang S, Li C, Xu X, Zhou G. Effect of fasting on energy metabolism and tenderizing enzymes in chicken breast muscle early postmortem. Meat Sci 2013;93:865-72.   DOI
4 Zhang L, Yue H, Wu S, et al. Transport stress in broilers. II. Superoxide production, adenosine phosphate concentrations, and mRNA levels of avian uncoupling protein, avian adenine nucleotide translocator, and avian peroxisome proliferator-activated receptor-${\gamma}$ coactivator-$1{\alpha}$ in skeletal muscles. Poult Sci 2010;89:393-400.   DOI
5 Liu Y, Li J, Li Y, et al. Effects of dietary supplementation of guanidinoacetic acid and combination of guanidinoacetic acid and betaine on postmortem glycolysis and meat quality of finishing pigs. Anim Feed Sci Technol 2015;205:82-9.   DOI
6 Clarke FM, Shaw FD, Morton DJ. Effect of electrical stimulation post mortem of bovine muscle on the binding of glycolytic enzymes. Biochem J 1980;186:105-9.   DOI
7 Lametsch R, Essen-Gustavsson B, Jensen-Waern M, Lundstrom K, Lindahl G. Postmortem changes in phosphorylation of metabolic enzymes in relation to the RN-genotype. In: 55th ICoMST; 2009.
8 Odland LM, Heigenhauser GJ, Wong D, Hollidge-Horvat MG, Spriet LL. Effects of increased fat availability on fat-carbohydrate interaction during prolonged exercise in men. Am J Physiol Regul Integr Comp Physiol 1998;274:R894-R902.   DOI
9 Wilmore JH, Costill DL. Physiology of sport and exercise. 3nd edn. Champaign, IL: Human Kinetics; 2005. pp. 116-45.
10 Gatenby RA, Gillies RJ. Why do cancers have high aerobic glycolysis? Nat Rev Cancer 2004;4:891-9.   DOI
11 Zhao J, Zhao G, Jiang R, et al. Effects of diet-induced differences in growth rate on metabolic, histological, and meat-quality properties of 2 muscles in male chickens of 2 distinct broiler breeds. Poult Sci 2012;91:237-47.   DOI
12 Ribeiro W, Valberg S, Pagan J, Gustavsson B. The effect of varying dietary starch and fat content on serum creatine kinase activity and substrate availability in equine polysaccharide storage myopathy. J Vet Intern Med 2004;18:887-94.   DOI
13 Van de Moortel L, Speeckaert MM, Fiers T, et al. Low serum creatine kinase activity is associated with worse outcome in critically ill patients. J Crit Care 2014;29:786-90.   DOI
14 Boden G, Shulman G. Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and ${\beta}$-cell dysfunction. Eur J Clin Invest 2002;32:14-23.   DOI
15 Westerblad H, Bruton JD, Katz A. Skeletal muscle: energy metabolism, fiber types, fatigue and adaptability. Exp Cell Res 2010;316:3093-9.   DOI
16 Yu C, Li Y, Zhang B, et al. Suppression of mTOR signaling pathways in skeletal muscle of finishing pigs by increasing the ratios of ether extract and neutral detergent fiber at the expense of starch in isoenergetic diets. J Agric Food Chem 2016;64:1557-64.   DOI
17 Storey KB. Anoxia tolerance in turtles: metabolic regulation and gene expression. Comp Biochem Physiol Part A Mol Integr Physiol 2007;147:263-76.   DOI
18 Borges P, Valente LM, Veron V, et al. High dietary lipid level is associated with persistent hyperglycaemia and downregulation of muscle Akt-mTOR pathway in Senegalese sole (Solea senegalensis). PLoS One 2014;9:e102196.   DOI
19 Semenza GL. Defining the role of hypoxia-inducible factor 1 in cancer biology and therapeutics. Oncogene 2010;29:625-34.   DOI
20 Silva APP, Alves GG, Araujo AH, Sola-Penna M. Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle. An Acad Bras Cienc 2004;76:541-8.   DOI
21 Mason SD, Howlett RA, Kim MJ, et al. Loss of skeletal muscle HIF-$1{\alpha}$ results in altered exercise endurance. PLoS Biol 2004;2:e288.   DOI
22 Bendall JR. The shortening of rabbit muscles during rigor mortis: its relation to the breakdown of adenosine triphosphate and creatine phosphate and to muscular contraction. J Physiol 1951;114:71-88.
23 Scheffler TL, Gerrard DE. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci 2007;77:7-16.   DOI
24 Henckel P, Karlsson A, Jensen MT, Oksbjerg N, Petersen JS. Metabolic conditions in porcine longissimus muscle immediately pre-slaughter and its influence on peri-and post mortem energy metabolism. Meat Sci 2002;62:145-55.   DOI
25 Parra J, Pette D. Effects of low-frequency stimulation on soluble and structure-bound activities of hexokinase and phosphofructokinase in rat fast-twitch muscle. Biochim Biophys Acta 1995;1251:154-60.   DOI
26 Lushchak VI. Interaction of lactate dehydrogenase with structural cell components: possible physiological significance. Biokhimiia 1992;57:1142-54.
27 Forlemu NY. Waingeh VF, Ouporov IV, Lowe SL, Thomasson KA. Theoretical study of interactions between muscle aldolase and F‐actin: Insight into different species. Biopolymers 2007;85:60-71.   DOI
28 Semenza GL, Roth PH, Fang H-M, Wang GL. Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J Biol Chem 1994;269:23757-63.
29 Dekanty A, Lavista-Llanos S, Irisarri M, Oldham S, Wappner P. The insulin-PI3K/TOR pathway induces a HIF-dependent transcriptional response in Drosophila by promoting nuclear localization of HIF-${\alpha}$/Sima. J Cell Sci 2005;118:5431-41.   DOI
30 Jaakkola P, Mole DR, Tian Y-M, et al. Targeting of HIF-${\alpha}$ to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science 2001;292:468-72.   DOI
31 Wu R, Smeele KM, Wyatt E, et al. Reduction in hexokinase II levels results in decreased cardiac function and altered remodeling after ischemia/reperfusion InjuryNovelty and significance. Circ Res 2011;108:60-9.   DOI
32 Gottlob K, Majewski N, Kennedy S, et al. Inhibition of early apoptotic events by Akt/PKB is dependent on the first committed step of glycolysis and mitochondrial hexokinase. Genes Dev 2001;15:1406-18.   DOI
33 Weidemann A, Breyer J, Rehm M, et al. HIF-$1{\alpha}$ activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells. J Cell Commun Signal 2013;11:80.   DOI
34 Bereiter-Hahn J, Stübig C, Heymann V. Cell cycle-related changes in F-actin distribution are correlated with glycolytic activity. Exp Cell Res 1995;218:551-60.   DOI
35 Li Y, Li J, Zhang L, et al. Effects of dietary energy sources on post mortem glycolysis, meat quality and muscle fibre type transformation of finishing pigs. PLoS One 2015;10:e0131958.   DOI
36 Chen JX, Stinnett A. Ang-1 gene therapy inhibits hypoxia-inducible factor-1alpha (HIF-1alpha)-prolyl-4-hydroxylase-2, stabilizes HIF-1alpha expression, and normalizes immature vasculature in db/db mice. Diabetes 2008;57:3335-43.   DOI