• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.374-381
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• 2015

This study aimed to evaluate the effects of vitamin E (VE), ferulic acid (FA) and their combination supplementation on meat quality and antioxidant capacities of finishing pigs. Sixty barrows were randomly allocated to four experimental diets using a $2{\times}2$ factorial arrangement with 2 VE supplemental levels (0 or 400 mg/kg) and 2 FA supplemental levels (0 or 100 mg/kg) in basal diets. After 28 days, six pigs per treatment were slaughtered. The results showed that VE supplementation increased loin eye area of pigs (p<0.05) and FA supplementation increased $pH_{45min}$ value (p<0.05). The interaction of $FA{\times}VE$ was observed in shear force of longissimus dorsi muscle (p<0.05). Moreover, supplementation with VE decreased hepatic and sarcous malondialdehyde (MDA) content, increased hepatic glutathione (GSH) content and sarcous glutathione peroxidase (GSH-Px) activity (p<0.05). Additionally, supplementation with FA increased hepatic GSH-Px activity and decreased sarcous MDA content (p<0.05). However, dietary treatment did not affect the expression of genes related to nuclear factor, erythroid 2-like 2 (NFE2L2) pathway. These results suggest that dietary FA and VE could partially improve meat quality and antioxidant capacity of finishing pigs, but not by activating NFE2L2 pathway under the normal conditions of farming.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.11
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• pp.42-48
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• 2020

The agricultural tractor is a multi-purpose vehicle, which is frequently used in the agricultural field. It must be highly reliable in terms of human safety. Design and analysis of agricultural tractors must be performed using actual agricultural workload to maintain high reliability. Additionally, the frequency with which various components and systems are used must also be taken into consideration. In this study, a tractor is built to measure its workload in the actual field. Further, the measured load was analyzed for various farming tasks. The range of ratios of consumed power to engine power was measured to be 42.6%-87.2%, 75.1%-97%, 26.5%-59.2% for a plow, rotary, and harvest tasks, respectively. The results were fed into a transmission simulation model to analyze the strength and life of the transmission components. We conclude that a more reliable product can be constructed by incorporating the transmission analyses using the actual load.

• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• The Korean Journal of Pesticide Science
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• v.7 no.4
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• pp.258-263
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• 2003

In order to get the actual pesticide usage data for fruit crops at farmer's gate, this survey was carried out on the growers of 6 representative fruit crops such as apple, citrus, grape, peach, pear and persimmon, in Korea. One hundred fifty farmers were selected in main production areas to record their pesticide usage during the growing seasons. Pesticides were sprayed in pear 15.2 times/year and persimmon 7 times/year. Pesticide input amount per unit for fruits was 39.5 for citrus, 22.1 for apple, 21.5 for pear, 11.9 for peach, 10.8 for persimmon and 6.7 a.i. kg/ha for grape, respectively. The amounts of pesticides sprayed in 2002 except for peach and pear tend to decrease compared with those in 1999. The major fungicides used were mancozeb, propineb, dithianon, etc., while the major insecticides were fenitrothion, chlorpyrifos, methidathion, etc. Total active ingredients of pesticides used for six fruit crops amounted to 18.3% of total sales amount of pesticides for horticulture in 2002. The amounts of pesticides sprayed on citrus, grape, peach and pear in Korea was less than those in U.S.A., excepting those in apple.

• Food Science of Animal Resources
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• v.41 no.4
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• pp.650-663
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• 2021

The objective of this study was to examine the relationship between meat quality attributes and the changes of sarcoplasmic protein acetylation and myofibrillar protein acetylation in lamb longissimus thoracis et lumborum muscles at different postmortem phases. Protein acetylation, color, pH, shear force, myofibril fragmentation index and cooking loss were measured. The total level of acetylated sarcoplasmic proteins showed a negative relation with pH, a positive relation with a^*, b^* and cooking loss at the pre-rigor phase. Sarcoplasmic proteins acetylation affected postmortem pH by regulating glycolysis, which in turn affects color and cooking loss. The total level of acetylated myofibrillar proteins showed a positive relation with shear force at the pre-rigor phase. Myofibrillar proteins acetylation affected meat tenderness by regulating muscle contraction. This study indicated that acetylation played a regulatory role of meat color, water-holding capacity, and tenderization process at early postmortem.

• Journal of the Ergonomics Society of Korea
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• v.30 no.4
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• pp.509-515
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• 2011

Objective: The study was performed to suggest factors to consider when making or selecting protective clothing to enhance farmers' health. The purpose of this study was to review and summarize the agricultural safety technology on clothing including hat, gloves, and shoes. Background: The farmers' work clothing has to meet a multitude of requirement. Risk factors in farm-work were identified by previous researches. Agricultural safety clothing needs to be introduced. Method: We reviewed previous papers regarding the agricultural workers' health, safety clothing to protect body from excessive heat and humid environment, ultraviolet(UV) protection, and hazardous body working postures. Also the UV cut effect, pesticideproof clothing, and preventable farm work related musculoskeletal disorders were discussed. Results: The agricultural safety clothing needs to be developed to provide comfort and to be resistant against heat. The UV protection function on skin and eyes has not been fully researched in safety clothing studies yet. Conclusions and Application: The farmers' work-related body posture needs to be studied in order to make and design agricultural safety clothing. The safety issues on agricultural work shoes are also needed to be included in future studies.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.71-77
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• 2017

Objective: This experiment was conducted to investigate the effects of dietary supplementation with marigold extract on growth performance, pigmentation, antioxidant capacity and meat quality in broiler chickens. Methods: A total of 320 one-day-old Arbor Acres chickens were randomly divided into 5 groups with 8 replicates of 8 chickens each. The chickens of control group were fed with basal diet and other experimental groups were fed with basal diet supplemented with 0.075%, 0.15%, 0.30%, and 0.60% marigold extract respectively (the corresponding concentrations of lutein were 15, 30, 60, and 120 mg/kg). Results: The results showed that marigold extract supplementation increased the yellowness values of shank, beak, skin and muscle and the redness ($a^*$) value of thigh muscle (linear, p<0.01). Marigold extract supplementation significantly increased the total antioxidant capacity, and the activities of superoxide dismutase in liver and thigh muscle (linear, p<0.01) and significantly decreased the malondialdehyde contents of liver and thigh muscle (linear, p<0.01). Marigold extract supplementation significantly decreased the drip loss and shear force of thigh muscles (linear, p<0.01). There was no significant effect on growth performance with marigold extract supplementation. Conclusion: In conclusion, dietary supplementation of marigold extract significantly increased the yellowness values of carcass, antioxidant capacity and meat quality in broiler chickens.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.569-575
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• 2017

Objective: The objective of this research was to study the electrical properties and quality of frozen-thawed chicken breast meat and to investigate the relationship between these parameters at different times of frozen storage. Methods: Thawed samples of chicken breast muscles were evaluated after being kept in frozen storage at $-18^{\circ}C$ for different periods of time (1, 2, 3, 4, 5, 6, 7, and 8 months). Results: The results showed that water-holding capacity (WHC) and protein solubility decreased while thiobarbituric acid-reactive substances content increased with increasing storage time. The impedance module of samples decreased during 8-month frozen storage. Pearson correlation coefficients showed that the impedance change ratio (Q value) was significantly (p<0.05) related to pH, color, WHC, lipid oxidation and protein solubility, indicating a good relationship between the electrical properties and qualities of frozen-thawed chicken breast meat. Conclusion: Impedance measurement has a potential to assess the quality of frozen chicken meat combining with quality indices.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.12
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• pp.1764-1772
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• 2017

Objective: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. Methods: Seventy-two barrow ($Duroc{\times}Landrace{\times}Yorkshire$, DLY) pigs ($65.0{\pm}2.0kg$) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected. Results: Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05). Conclusion: Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.11 no.3
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• pp.223-229
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• 2017

This study analyzed any accidents occurred by agricultural machines and comprehended the safety for trends in the current research. The researches pertaining to the safety for agricultural machines are identified by two different methods: scrutinizing the statistical data for accidents and suggesting solutions to ways of an investigation. Other researches related to investigations for the accidents by agricultural machines are planned on the national level and the broad terms of an accident of agricultural activities. The studies are conducted for the prevention and control of accidents about the requirement analysis as well as administrative, educational and financial policy in accordance with the different kinds of the agricultural machines. In addition, for the better responses to injuries caused by the agricultural machines, more researches about the function and ability of elders are much needed.