• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.25-30
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• 2015

Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.272-276
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• 1998

Sheep and goats in Pakistan have not been able to produce to the best of their potential. This may primarily be attributed to under feeding and malnutrition. Ranges have been depleted due to overgrazing and mismanagement and are not in position to feed the existing small ruminant population. To overcome the shortage of good quality fodder and balanced feed supply, the alternate means like cereal straws and other crop residues are being commonly used. Sunflower crop residues like stalks and heads provide a good quality forage for livestock. These crop byproducts are rich in crude protein and lower in crude fibre. Their inclusion in the diet of small ruminants at 20, 30 and 40 percent levels in ration has shown significantly (p < 0.05) increased feed intake and weight gain. Daily feed intake was 1,130, 1,180 and 1,750 g for sunflower crop residue, soybean crop residue and wheat straw, respectively, when added at the rate of 20 percent in the ration. The drymatter digestibility of sunflower, soybean crop residues and wheat straw was also comparable. The maximum performance of the animals was observed at the 20 percent level of inclusion of sunflower crop residue in the diet.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.2
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• pp.79-84
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• 2015

A new cecropin-like antimicrobial peptide (Px-CLP) gene was isolated from the immunechallenged larvae of the swallowtail butterfly, Papilio xuthus, by employing annealing control primer (ACP)-based GeneFishing PCR. The full-length cDNA of Px-CLP is 310 nucleotides encoding a 70 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propeptide, a presumed 37-residue mature peptide, and an uncommon 7-residue acidic pro-region at the C-terminus. The deduced amino acid sequence of Px-CLP showed significant identities with other Lepidopteran cecropin D type peptides. RT-PCR revealed that the Px-CLP transcript was detected at significant level after injection with bacterial lipopolysaccharide (LPS). The peptides with or without C-terminal acidic sequence region were synthesized on-solid phage and submitted to antibacterial activity assay. The synthetic 37-mer peptide (Px-CLPa), which removed C-terminal acidic sequence region, was showed exclusively antibacterial activity against E. coli ML35; meanwhile, a 44-mer peptide (Px-CLPb) with C-terminal acidic peptide region was not active. This result suggests that Px-CLP is produced as a larger precursor containing a C-terminal pro-region that is subsequently removed by C-terminal modification.

• Korean Journal of Environmental Agriculture
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• v.41 no.1
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• pp.1-8
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• 2022

BACKGROUND: The pesticide residue in rotational crop is one of the main concerns to agricultural products because it has the potentiality of violating positive list system (PLS). Thus, the crops used for the rotational cultivation should be considered the pesticide residue patterns to meet the PLS guideline. In this study, we evaluated the residue patterns of fungicide azoxystrobin in radish based on plant back interval (PBI) experiment. METHODS AND RESULTS: Azoxystrobin was treated onto greenhouse soil at 217 g a.i./10a in two different regions. Radishes were sown onto the soil 30 and 60 days after azoxystrobin treatment. The soil and plant samples were subjected to a modified QuEChERS method and LC/MS/MS analyses to determine the residues of azoxystrobin. The methods were validated to meet the guidelines of the pesticide residue analysis recommended by the Rural Development Administration, Republic of Korea. Azoxystrobin was dissipated significantly in soil during the experimental period and found as a level less than 0.01 mg/kg in radish 30 and 60 days after treatment. Azoxystrobin residues in radish samples were lower than the maximum residue limit (MRL) for root vegetables. CONCLUSION(S): This study suggests 30 days as a PBI for rotational cultivation of radish in greenhouse soil that had been treated with azoxystrobin at a level of 217 g a.i./10a.

• Korean Journal of Environmental Agriculture
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• v.31 no.2
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• pp.152-156
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• 2012

BACKGROUND: This research has investigated the residue patterns of insecticide flubendiamide on three species of peaches with different surface forms, and the residue amounts of them when mixed with a spreader. METHODS AND RESULTS: Pesticide used for field application on peaches was 20% flubendiamide of suspension concentrate(SC) and was sprayed at a recommended rate. The residue amounts of flubendiamide in peach were analyzed by HPLC equipped with UV detector. After the observation with a microscope, the rank of fuzz amount on peach's surface was Kurakatawase, Wolmi in descending order and Cheonhong did not have any fuzz. The residue amounts of flubendiamide were 0.54 mg/kg for Kurakatawase, 0.43 mg/kg for Wolmi and 0.10 mg/kg for Cheonhong, respectively. When flubendiamide was used with a spreader, polyoxy ethylene methylpoly siloxane, the residue amount for Kurakatawase barely changed at 0.55 mg/kg regardless of mixing with a spreader, and at 0.53 mg/kg for Wolmi. In Cheonhong, the residue amount was 0.48 mg/kg, which increased by 4.8 times due to the use of a spreader. CONCLUSION: This result indicates that the residue amounts of flubendiamde were affected by the surface forms of peaches, and in the presence of a spreader the residue amount did not increase in fuzzy species, but was affected greatly for species without fuzz.

• Magazine of the Korean Society of Agricultural Engineers
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• v.40 no.4
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• pp.85-93
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• 1998

In the 2nd year study of a 5 year project to evaluate the soil quality and develop the best management practices for mountaineous soils, 11 runoff plots were treated and monitored with respect to physical property of the soil, runoff and sediment discharge, and the following results were obtained. 1. Bulk density and porosity did not show any siginificant difference between experimental treatments. 2. Runoff was basically dependent on the soil's physical property and tillage. Up-and-down plots showed the highest runoff while contour plots the lowest runoff. 3. Sediment yield in the mountaineous soils was directly related to tillage and residue cover. Residue covered plots showed the lowest sediment yield and up-and-down plots the highest sediment yield. And it is recommended that the best management practices using till_age and residue cover for the mountaineous soils must be developed to protect soil quality and maintain agricultural productivity.

• Korean Journal of Environmental Agriculture
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• v.41 no.3
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• pp.153-157
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• 2022

BACKGROUND: The residue dissipation pattern of pesticides for agricultural products during the pre-harvest period after the final application is important to prevent the maximum residue limit (MRL) violations in domestic and export markets. The MRL violations of carbendazim are observed more often in chamnamul by pesticide residue management surveys by the Ministry of Food and Drug Safety. The residue level at the pre-harvest interval (PHI) and the residue dissipation constant from the critical good agricultural practice (cGAP) trials could be estimated to meet the MRL and pose a health risk to consumers. METHODS AND RESULTS: Chamnamuls were harvested at 0, 1, 3, 5, 7, 10, and 14 days after application of carbendazim in accordance with critical GAP. The residue analysis in chamnanul was performed by HPLC-DAD with the C₁₈ column. The limit of quantitation of carbendazim was 0.04 mg/kg, and the recoveries were 74.4 - 95.8% at the two spiked levels (LOQ and 10LOQ) of carbendazim. The dissipation rates in chamnamul were calculated from the residues at the sampling days by statistical method at a 95% confidence level. The biological half-lives of residual carbendazim in the field trials 1 and 2 were 4.9 and 4.4 days, respectively. CONCLUSION(S): In this dissipation study, the residue concentrations at the recommended PHI were higher than the established MRL in Korea. Therefore, the MRL is proposed based on the residue data sets from the trials conducted at the same cGAP and the dietary exposure assessment.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.308-314
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• 2012

In order to monitor the residual pesticides in environment friendly agricultural commodities, fruits and fruiting vegetables. Twenty-five agricultural commodities were collected twice in May and August 2011 from nine environment friendly agricultural commodities-selling supermarkets and retail stores located in eight major cities in Korea. The number of each agricultural commodity collected, 555 samples in total, was 152 organic agricultural products, 202 pesticide-free agricultural products and 201 low-pesticide agricultural products. Pesticide residues in samples were analyzed by multiresidue method for 245 pesticides using a GC-ECD/NPD and an HPLC-DAD/FLD and the peaks suspected as pesticides were identified with a GC/MSD. As a result of pesticide residue analysis, three pesticides, bifenthrin, EPN and chlorpyrifos, were detected from four samples including apple, representing a detection rate of 0.72%. The residue levels of the four pesticide-detected samples were less than their maximum residue limits (MRLs) but one pesticide EPN detected from pear exceeded its legible criterion of one twentieth MRL. Estimated daily intakes of the pesticides detected from fruits and fruiting vegetables were less than 0.76% of their maximum permissible intake.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.4
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• pp.502-511
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• 1997

This study was to Investigate the consumer's understanding and purchasing foods, which were produced by organic agricultured method. The results of this study were as follows: 1. When shopping foods, the items which were deeply considered were taste of family and food safety. 2 Contaminants which were considered to be dangerous were, in decreasing order, agricultural chemical residue, food additives, environmental contaminant, and microbial hazard. Consumer's perception to agricultural chemical residue In foods was 'be much serious' and over 4.0 by Likert 5 scale. 3. Among of characteristics which was associated with organic farmming foods, taste and nutrition were considered the least important characteristic, whereas safety and good health were considered the most important characteristic. 4. The foods which were thought to be polluted by agricultural chemical residue were, in decreasing order fruit, vegetable, cereal, egg, meat, milk, and fish & shellfish. 5. The respondants' purchasing degree for organic farmming foods was 'often purchase' and 3.44 by Likert 5 scale. These result showed significant difference for age(p＜0.05), for education level (p＜0.05), for income(p＜0.05) and for food expenditure(p＜0.05), respectively 6. The type of organic farmming food which frequently purchased were, in decreasing order, vegetable. fruit, egg, cereal, milk, and meat. The reasons why consumer purchased organic farmming food were, in decreasing order, no pesticide/therbicides, no artifical fertilizer, no growth regulators, residue free, and good quality. 7. 78% of respondants Indicated that they bought organic farmming food in this year increasely or same as last year and 91.9% of respondants Indicated that they plan to buy organic farmming foods in next year.

• Bulletin of the Korean Chemical Society
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• v.28 no.3
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• pp.369-372
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• 2007

A fast and simple high-performance liquid chromatography (HPLC) method for the simultaneous determination of four pesticides having fungicide properties has been proposed for Panax ginseng, C. A. Meyer grown for 4, 5, or 6 years. Analytical separation was performed on C18 columns using ultraviolet detector under gradient conditions. Spiked blank samples were used as standards to counteract the matrix effect observed in the chromatographic determination. The HPLC response for all pesticides was linear, with determination coefficients > 0.9986. The average rate of recovery for pesticides spiked with 2 fortification levels was > 72% with relative standard deviations < 9%. The limits of quantification (LOQ) ranged from 0.03 to 0.16 ppm. These LOQs were lower than the respective maximum residue limits (MRL) established by the Korean Food and Drug Administration (KFDA), except for cyazofamid. The proposed method was used to determine pesticide residue levels in samples of ginseng obtained from Jeonnam Province (Republic of Korea). None of the pesticides were found in ginseng samples grown for 4, 5, or 6 years.