Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Residues of Fungicide Azoxystrobin in Radish based on Plant Back Interval Experiment

식물후방식재기간(PBI) 시험에 기반한 살균제 Azoxystrobin의 알타리무 중 잔류량 평가

  • Yoon, Ji Hyun (Department of Agricultural and Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lim, Da Jung (Department of Agricultural and Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Seon Wook (Department of Agricultural and Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, In Seon (Department of Agricultural and Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
  • 윤지현 (전남대학교 농업생명과학대학 농생명화학과) ;
  • 임다정 (전남대학교 농업생명과학대학 농생명화학과) ;
  • 김선욱 (전남대학교 농업생명과학대학 농생명화학과) ;
  • 김인선 (전남대학교 농업생명과학대학 농생명화학과)
  • Received : 2022.03.01
  • Accepted : 2022.03.18
  • Published : 2022.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: The pesticide residue in rotational crop is one of the main concerns to agricultural products because it has the potentiality of violating positive list system (PLS). Thus, the crops used for the rotational cultivation should be considered the pesticide residue patterns to meet the PLS guideline. In this study, we evaluated the residue patterns of fungicide azoxystrobin in radish based on plant back interval (PBI) experiment. METHODS AND RESULTS: Azoxystrobin was treated onto greenhouse soil at 217 g a.i./10a in two different regions. Radishes were sown onto the soil 30 and 60 days after azoxystrobin treatment. The soil and plant samples were subjected to a modified QuEChERS method and LC/MS/MS analyses to determine the residues of azoxystrobin. The methods were validated to meet the guidelines of the pesticide residue analysis recommended by the Rural Development Administration, Republic of Korea. Azoxystrobin was dissipated significantly in soil during the experimental period and found as a level less than 0.01 mg/kg in radish 30 and 60 days after treatment. Azoxystrobin residues in radish samples were lower than the maximum residue limit (MRL) for root vegetables. CONCLUSION(S): This study suggests 30 days as a PBI for rotational cultivation of radish in greenhouse soil that had been treated with azoxystrobin at a level of 217 g a.i./10a.

Keywords

Acknowledgement

This work was funded by the Rural Development Administration (Grants PJ015053 and PJ015277).

References

  1. OECD (2016) Draft guidance document on residues in rotational crops. OECD Environ. Health Safety Public, 1, 1-46. Available at https://www.oecd.org/env/ehs/testing/OECDRotationalCropGuidelineDraftVersion_19%July2016..AEpdf.
  2. Anesiadis T, Karaoglanidis S, Tzavella-Klonari K (2003) Protective, curative and eradicant activity of the strobilurin fungicide azoxystrobin against Cercospora beticola and Erysiphe betae. Journal of Phytopathology, 151, 647-641. https://doi.org/10.1046/j.1439-0434.2003.00780.x.
  3. Kanetis L (2007) Comparative efficacy of the new postharvest fungicides azoxystrobin, fludioxonil, and pyrimethanil for managing citrus green mold. Plant Disease, 91, 1502-1511. https://doi.org/10.1094/PDIS-91-11-1502.
  4. Wong FP, Wilcox WE (2001) Comparative physical modes of action of azoxystrobin, mancozeb, and metalaxyl against Plasmopara viticola (Grapevine Downy Mildew). Plant Disease, 85, 649-656. https://doi.org/10.1094/PDIS.2001.85.6.649.
  5. Bertelsen JR, Neergaard ED, Smedegaard-Peterse V (2001) Fungicidal effects of azoxystrobin and epoxiconazole on phyllosphere fungi, senescence and yield of winter wheat. Plant Pathology, 50, 190-205. https://doi.org/10.1046/j.1365-3059.2001.00545.x.
  6. Gajbhiye VT, Gupta S, Mukherjee I, Singh SB, Singh N, Dureja P, Kumar Y (2011) Persistence of azoxystrobin in/on grapes and soil in different grapes growing areas of India. Bulletin of Environmental Toxicology and Contamination, 86, 90-94. https://doi.org/10.1007/s00128-010-0170-2.
  7. Adetutu EM, Ball AS, Osborn AM (2008) Azoxystrobin and soil interactions: degradation and impact on soil bacterial and fungal communities. Journal of Applied Microbiology, 105, 1777-1790. https://doi.org/doi:10.1111/j.1365-2672.2008.03948.x.
  8. Purnamaab I, Malhatac F, Jaikaewa P, Watanabea H, Noegrohatib S, Rusdiarsob B, Ahmed MT (2014) Degradation profile of azoxystrobin in Andisol soil: laboratory incubation. Toxicological and Environmental Chemistry, 96, 1141-1152. https://doi.org/10.1080/02772248.2015.1015297.
  9. Ghosh RK, Singh N (2009) Effect of organic manure on sorption and degradation of azoxystrobin in soil. Journal of Agricultural and Food Chemistry 57, 632-636. https://doi.org/10.1021/jf802716f.
  10. Anastassiades M, Lehotay SJ (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. Journal of AOAC International, 86, 412-431. https://doi.org/10.1093/jaoac/86.2.412.
  11. SANTE Guidance Document on Analytical Quality Control and Validation Procedures for Pesticide Analysis in Food and Feed; SANTE/12682/2019, Implemented by 01.01.2020; European Commission Directorate General for Health and Food Safety: Brussels, Belgium, 2020.
  12. Dong M, Nie D, Tang H, Rao Q, Qu M, Wang W, Han L, Song W, Han Z (2015) Analysis of amicarbazone and its two metabolites in grains and soybeans by liquid chromatography with tandem mass spectrometry. Journal of Separation Science, 38, 2245-2252. https://doi.org/10.1002/jssc.201500265.
  13. Lim DJ, Kim SW, Kim YE, Yoon JH, Cho HJ, Shin BG, Kim HY, Kim IS (2021) Plant-back intervals of imicyafos based on its soil dissipation and plant uptake for rotational cultivation of lettuce and spinach in greenhouse. Agriculture, 11, 495, 1-10. https://doi.org/10.3390/agriculture11060495.
  14. Hwang K. Yoo SC, Le S, Moon JK (2018) Residue level of chlorpyrifos in lettuces grown on chlorpyrifos-treated soils. Applied Science, 8, 2343, 1-10. https://doi.org/10.3390/app8122343.
  15. Hwang J, Zimmerman A, Kim J (2018) Bioconcentration factor-based management of soil pesticide residues: Endosulfan uptake by carrot and potato plants. Science and Total Environment, 627, 514-522. https://doi.org/10.1016/j.scitotenv.2018.01.208.
  16. Ju C, Zhang H, Yao S, Dong S, Cao D, Wang F, Fang H, Yu Y (2019) Uptake, translocation, and subcellular distribution of azoxystrobin in wheat plant (Triticum aestivum L.). Journal of Agricultural and Food Chemistry, 67, 6691-6699. https://doi.org/10.1021/acs.jafc.9600361.
  17. European Food Safety Authority (2012) Reasoned opinion on the modification of the existing MRLs for azoxystrobin in lettuce, spinach, celery, cardoon, spices and rhubarb. EFSA Journal, 10, 2991, 27 pp. https://doi.org/10.2903/j.efsa.2012.2991.
  18. European Food Safety Authority (2008) Reasoned opinion of EFSA prepared by the Pesticides Unit (PRAPeR) on setting of an import tolerance for azoxystrobin in passion fruits. EFSA Journal, 209, 25. https://doi.org/10.2903/j.efsa.2008.209r.
  19. European Food Safety Authority (2009) Reasoned opinion of EFSA prepared by the Pesticides Unit (PRAPeR) on the modification of the existing MRL for azoxystrobin in swedes. EFSA Journal, 1308, 20. https://doi.org/10.2903/j.efsa.2009.1308.
  20. European Food Safety Authority (2009) Reasoned opinion of EFSA prepared by the Pesticides Unit (PRAPeR) on the modification of the existing MRL for azoxystrobin in cardoon. EFSA Journal, 1362, 23. https://doi.org/10.2903/j.efsa.2009.1362.