• Biomolecules & Therapeutics
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• v.31 no.2
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• pp.210-218
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• 2023

Prostate cancer is the fifth leading cause of cancer-related mortality in men, primarily because of treatment resistance, recurrence, and metastasis. In the present study, we investigated the role of paracrine interleukin-8 (IL-8) in the antagonistic expression of IL-8 and androgen receptor (AR), and the contribution of IL-8 to prostate cancer aggressiveness. In hormone-responsive LNCaP cells that do not express IL-8, recombinant IL-8 treatment significantly increased expressions of IL-8, CXC chemokine receptor 2 (CXCR2), matrix metalloproteinase (MMP)-2/9, Snail, and vimentin. IL-8 treatment significantly decreased AR and E-cadherin expression. IL-8-induced gene expression changes were suppressed by navarixin, a CXCR1/2 inhibitor, and gallein, a Gβγ inhibitor. In PC-3 androgen-refractory prostate cancer cells, IL-8 knockdown reduced expressions of CXCR2, MMP-2/9, Snail, and vimentin, and increased AR and E-cadherin expressions at the mRNA and protein levels. Co-culture with MEG-01 human megakaryocytic cells secreting high levels of IL-8 induced gene expression changes in both LNCaP and PC-3 cells, similar to those induced by IL-8 treatment. The altered gene expressions were accompanied by significant activation of transcription factor Snail in LNCaP and PC-3 cells. Treatment with the CXCR blocker navarixin inhibited the invasion of PC-3 cells but not LNCaP cells. However, invasion induced by MEG-01 was inhibited by navarixin in both LNCaP and PC-3 cells. The collective findings demonstrate that IL-8 enhances CXCR2 expression, which antagonistically regulates AR expression. More importantly, through changes in IL-8/CXCR2-regulated gene expression, IL-8 induces antiandrogen therapy resistance and epithelial-mesenchymal transition in prostate cancer.

• Journal of Chest Surgery
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• v.32 no.8
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• pp.726-731
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• 1999

Background: The distinction between non-invasive and invasive or thymic carcinoma has been severely compromised by lack of objective morphological criteria. A reliable biological marker of tumor aggressiveness is, therefore, mandatory for predicting tumor behavior. Material and Method: Thirty thymic epithelial tumors, including 7 non-invasive thymoma, 10 invasive thymoma, and 13 thymic carcinoma of the Rosai's classification; and 5 stage I, 7 stage II, 2 stage III, and 3 stage IVa of the Masaoka stage of thymoma were investigated for expression of bcl-2 and p53 proteins by immunohistochemistry. Result: The thymic epithelial cells showed positive immunostain for bcl-2 in 0 (0%), 3 (30%), 8 (61.5%) of categories in the Rosai's classification respectively and in 0 (0%), 1 (14.3%), 2 (100%), 0 (0%) of stage I, II, III, IVa of the Masaoka stage respectively. Thymic carcinoma, and high stage thymoma had significantly higher proportion of bcl-2 expression than thymoma (p=0.021) and low stage thymoma (p=0.011). However, p53 showed no correlation with the histological subtypes nor with clinical aggressiveness. Bcl-2 expression appeared to be positively correlated with p53 immunoactivity (p=0.007, kappa=0.525). Conclusion: These date indicate that bcl-2 expression correlates with aggressiveness in thymic epithelial tumors, but further studies on mutation of p53 protein is necessary because bcl-2 expression appeared to be positively correlated with p53 immunoactivity.

• Journal of Music and Human Behavior
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• v.2 no.2
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• pp.33-56
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• 2005

The purpose of this study is to measure the effect of musical activities on children's aggressiveness using percussion playing through case studies and to present the therapeutic programs. Musical activities using percussion playing were organized for three aggressive children. Twenty-one small group sessions were conducted over seven weeks with 30 minutes given each session. Fourth-grade children involved in using Aggressiveness Measuring Tool for Teachers-revised by Gwak Geum-Joo(1992) was selected for case studies. Children's impulsiveness was also tested through self-test measuring tool for impulsiveness-revision of 16 questions used by Cho Hae Yeon (2001) and Lee Joo Shik (2003). As quantitative method, comparative analysis was made between the pre and post test results using measuring tools for aggressiveness and impulsiveness of children as well as between aggressive behaviors occurring in the initial stage of the first three sessions and in the latter stage of the last three sessions. Qualitative method was used at the same time to examine the effect of percussion playing on children. After the musical activities, child A showed reduced Aggressive Measuring Tool scores from 19 to 18, with child B from 23 to 19 and child C from 21 to 18. The results show that occurrence of aggressive behaviors were lowered in the post test. Impulsiveness Measuring Tool scores in the post test were decreased as well in all three children. During the music therapy programs, it was also observed that the frequency of the target behaviors in all three children has reduced more in the latter stage than the initial stage of music therapy. The qualitative findings indicate that the children experienced releasing stress through self-expression after percussion playing. These findings indicate therapeutic effectiveness of music therapy using on percussion playing in reducing aggressiveness of children as well as the effectiveness of percussion as a therapeutic intervention for aggressive children. These results can be adapted and reapplied by teachers in primary schools to approach children with behavior problems, and can present a useful therapeutic approach to therapists practicing in clinical environments.

• Korean Journal of Child Studies
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• v.28 no.1
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• pp.205-216
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• 2007

An internet addiction measure(Park et al., 2001) and an asocial behavior measure(K. J. Lee, 1997) were administered to 232 6th-grade students. Statistical analyses were by mean-difference scores and t-test. Results showed that all aspects of internet addiction(preoccupation, compulsive use, relapse, tolerance/dependence and impediments in daily life) were related to both the inner asocial behaviors of depression, social withdrawal and over-anxiety and the external asocial behaviors of aggressiveness, delinquency and hyperactivity. The only exception was that tolerance/dependence was not related to delinquency. Boys addicted to the internet were more socially withdrawn, aggressive, delinquent and hyperactive than girls. From the results, it can be suggested that internet education should be carried out from the elementary educational level.

• Transactions of the Korean Society of Automotive Engineers
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• v.12 no.3
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• pp.177-183
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• 2004

The out-of-positioned small female drivers are most likely to be injured during airbag deployment due to their stature and proximity to the steering wheel and airbag module. In order to investigate the injury mechanisms, some experimental studies with Hybrid III 5% female dummy and with female cadavers could be found from the open literatures. However, the given information from those experimental studies is quite limited to the standard conditions and might not be enough to estimate the airbag inflation aggressiveness regarding on the occupant responses and injury. In this study, a finite element analysis has been performed in order to investigate the airbag-induced injuries. A finite element 5% female human model in anatomical details has been developed. The validation results of the model are also introduced in this paper.

• Journal of Korean Neurosurgical Society
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• v.41 no.1
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• pp.53-56
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• 2007

Desmoplastic fibroma is one of the uncommon osseous tumors that present in the skull. Although classified as benign tumor, desmoplastic fibroma exhibits local aggressiveness and has a high potential for recurrence. The most common sites include metaphysis of long bones and mandible. Only 15cases have been described in the skull. We report the 16th case of desmoplastic fibroma of the skull.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.119-120
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• 2003

One of the most frequent defects in human cancer is the uncontrolled activation of the ras-signaling pathways. Elevated p21 ras expression is associated with tumor aggressiveness in breast cancer including the extent of invasion into fat tissues. infiltration into lymphatic vessels and tumor recurrence, We demonstrate that H-ras, but not N-ras, upregulates matrix metalloproteinase(MMP)-2 expression and induces invasive phenotype in MCF10A human breast epithelial cells. (omitted)

• BMB Reports
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• v.45 no.12
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• pp.719-723
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• 2012

A close association between the obesity hormone leptin and breast cancer progression has been suggested. The present study investigated the molecular mechanism for enhanced leptin expression in breast cancer cells and its functional significance in breast cancer aggressiveness. We examined whether leptin expression level is affected by the oncoprotein human epidermal growth factor receptor2 (HER2), which is overexpressed in ~30% of breast tumors. Here, we report, for the first time, that HER2 induces transcriptional activation of leptin in MCF10A human breast epithelial cells. We also showed that p38 mitogen-activated protein kinase signaling was involved in leptin expression induced by HER2. We showed a crucial role of leptin in the invasiveness of HER2-MCF10A cells using an siRNA molecule targeting leptin. Taken together, the results indicate a molecular link between HER2 and leptin, providing supporting evidence that leptin represents a target for breast cancer therapy.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.05a
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• pp.76-77
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• 2002

Ras expression has been suggested as a marker for tumor aggressiveness of breast cancer including the degrees of invasion and tumor recurrence. Expression of H-, K-, and N-ras is regulated in a tissue-specific manner and during development, indicating that ras proteins may have different cellular functions.(omitted)

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2003.10a
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• pp.54-54
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• 2003

Malignant fibrous histiocytomas (MFHs) is the most common type of soft tissue sarcoma in the old animal with a aggressiveness, a high local recurrence rate and significant metastatic rate, which associated with a poor prognosis. In most histologic and immunohistological studies, the tumor cells raised from a fibroblastic and/or myofibroblastic phenotype, presumably from undifferentiated mesenchymal cell origin. MFHs are usually firm and invasive, arising in the subcutis; metastasis depends on tumor grade (many are grade 3) [1,2]. The primary tumor cells are pleomorphic, varying in appearance from fusiform to round. Often nucleoli are prominent and irregular [5]. Extracellular amorphous eosinophilic material may be prominent and likely represents reactive collagen production by the tumor [5]. (omitted)