• 디지털융복합연구
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• 제17권8호
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• pp.257-264
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• 2019

본 연구는 부채마 70% 에탄올 추출물(DR)이 RBL-2H3 마스트 세포에서 항원에 의한 세포활성의 영향과 BALB/c 쥐에서 dinitrofluorobenzene에 의해 유도된 마스트 세포-매개 알레르기 접촉성피부염에 미치는 영향을 조사했다. DNP-HSA 항원과 Anti-DNP IgE 항체에 의해 활성 된 RBL-2H3 세포에서 에탄올 추출물은 급성 항원반응 표지인 ${\beta}$-hexosaminidase 분비와 히스타민 등을 억제하였고, 후기 항원반응 표지인 IL-4와 $TNF-{\alpha}$ 생성을 억압하였다. DNFB-유도 접촉성 알러지성 피부염 동물 모델에서 에탄올 추출물 처리는 귀 부기를 감소시키고 혈청 히스타민과 IL-4 분비를 억제시켰으며, DR 처리는 피부염 유발 부분에서 마스트 세포 유입을 효과적으로 방지하였다. 이러한 결과로, 에탄올 추출물은 아토피 피부염과 같은 마스트 세포-매개 알러지성 질병의 치료제로 사용할 수 있을 것이다.

• 인터넷정보학회논문지
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• 제20권2호
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• pp.93-100
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• 2019

최근 각종 사업 분야에서 기업들은 기존 메신저 플랫폼에 인공지능을 더하여 다양한 환경을 대상으로 챗봇 서비스 지원에 주력하고 있다. 취업알선 분야의 기관에서도 취업상담 서비스 품질 제고와 상담 인력 해소를 위해 챗봇 서비스를 요구한다. 일반적인 텍스트 기반 챗봇은 입력된 사용자 문장을 학습된 문장으로 분류하여 적합한 답변을 사용자에게 제공한다. 최근 소셜 네트워크 서비스의 활성화 영향으로 챗봇에 입력되는 사용자 문장은 단문으로 입력되는 경향이 있다. 따라서 단문 분류의 성능향상은 챗봇 서비스의 성능향상에 기여할 수 있다. 본 연구는 취업알선 챗봇을 위한 단문 분류 강화를 위해 기존 연구의 개념 정보뿐만 아니라 번역문 정보를 활용하는 방법인 T-EBOW (Translation-Extended Bag Of Words)를 제안한다. T-EBOW를 기계학습 분류 모델에 적용한 단문 분류의 성능은 기존 방법에 비해 우수한 성능 평가 결과를 보였다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2019년도 춘계학술대회
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• pp.413-415
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• 2019

본 논문에서는 공유경제를 위한 IoT 기반의 휴먼 인터랙티브 광고 서비스(IoT-HiAS, IoT-Human Interactive Advertising Service)를 구현한다. HiAS 디바이스가 웹캠을 통해 디바이스의 전방을 촬영하고 MobileNet을 사용한 SSD 모델을 통해 사람을 실시간으로 검출한다. 검출된 사람을 카운팅하여 설정한 기준 이상의 사람 수가 검출되면 빔 프로젝터를 통해 유휴자원에 광고를 재생한다. 광고가 재생됨과 동시에 디바이스 전방의 광고 시작 시점을 캡쳐한 이미지와 검출된 사람의 수를 IoT 클라이언트를 통해 HiAS 서버의 oneM2M 표준을 준용한 IoT 서버로 전송한다. 광고가 끝나면 디바이스 전방을 촬영하여 이미지를 IoT 서버로 전송한다. 전송받은 데이터를 HiAS 서버의 소셜 네트워크 서비스(SNS, Social Network Service) 에이전트를 통해 광고주 및 광고제작자에게 알림 메시지를 전송하여 IoT 기반의 휴먼 인터랙티브 광고 서비스를 구현하였다.

• 한국가족관계학회지
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• 제21권1호
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• pp.29-49
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• 2016

Objectives: The purpose of this study was aimed to examine the effect of self-esteem on life satisfaction and happiness among adolescents, and to verify moderated mediation of peer relations mediated by altruism and self-regulation. Method: The study referred to the 2015 Annual Report of the Korean Children and Youth Panel Survey. More precisely, report on the fourth year research process of the study cohort comprising 2,091 subjects was used to verify the research model. Overall data were analyzed by using SPSS 18.0 program and specifically, moderated mediation were verified by using SPSS Macro, as suggested by Preacher, etc. (2008). Results: As a result, firstly, the peer relations mediated the relationship between life satisfaction and self-esteem among adolescents. Secondly, altruism and self-regulation had moderated mediation effects between self-esteem and peer relations among adolescents. Conclusion: These results suggest that self-esteem affects on life satisfaction and happiness by mediation of peer relations among adolescents and altruism and self-regulation agent is applied positive adjustment to further promote the peer relations and increase life satisfaction among adolescents. Through these study results, the implications for counseling and suggestions for future research were discussed.

• 한국임상약학회지
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• 제31권1호
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• pp.27-34
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• 2021

Objective: To analyze osteoporosis treatment patterns and teriparatide prescription-associated factors in Korea by using a national health insurance claims database. Methods: We utilized the Health Insurance Review & Assessment Service National Patients Sample claims database to identify patients (aged ≥50 years) with at least one osteoporosis claim (International Classification of Disease 10th revision code: M80, M81, M82) and at least one prescription for osteoporosis medication (antiresorptive agents: bisphosphonates, selective estrogen receptor modulators, denosumab, and calcitonin; bone-forming agent: teriparatide) in 2018. Demographic characteristics and healthcare utilization patterns were analyzed. Factors associated with teriparatide prescriptions were assessed using a multivariate logistic regression model. Results: Records showed that 44,815 patients were prescribed osteoporosis medications in 2018; the percentage of patients prescribed each treatment was as follows: 86.6% bisphosphonates, 13.9% selective estrogen receptor modulators, 3.1% calcitonin, 2.1% denosumab, and 0.7% teriparatide. A greater proportion of patients prescribed teriparatide were ≥75 years (53.4% vs. 33.8%) and had fractures (63.9% vs. 12.8%) compared to the same for antiresorptives (p<0.001). Patients prescribed teriparatide had higher Charlson comorbidity index values (1.2±1.3 vs. 0.9±1.2) and were more frequently hospitalized (0.8±1.3 vs. 0.1±0.5) than those prescribed antiresorptives (p<0.001). Elderly patients (≥75 years old; adjusted OR=1.66; 95% CI 1.16-2.38) and those with fractures (adjusted OR=6.23; 95% CI 4.76-8.14) were more likely to be prescribed teriparatide than antiresorptives. Conclusion: Patients prescribed teriparatide were older and more likely to have severe osteoporosis than those prescribed antiresorptives.

• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.25-32
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• 2021

Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacteria can lead to severe septic shock. With the recent emergence of multidrug-resistant gram-negative bacteria and a lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. Here, to further examine its potency as an anti-endotoxin agent, we examined the anti-endotoxin activities of Pro9-3 and elucidated its mechanism of action. We performed a dye-leakage experiment and BODIPY-TR cadaverine and limulus amebocyte lysate assays for Pro9-3 as well as its lysine-substituted analogue and their enantiomers. The results confirmed that Pro9-3 targets the bacterial membrane and the arginine residues play key roles in its antimicrobial activity. Pro9-3 showed excellent LPS-neutralizing activity and LPS-binding properties, which were superior to those of other peptides. Saturation transfer difference-nuclear magnetic resonance experiments to explore the interaction between LPS and Pro9-3 revealed that Trp3 and Tlr7 in Pro9-3 are critical for attracting Pro9-3 to the LPS in the gram-negative bacterial membrane. Moreover, the anti-septic effect of Pro9-3 in vivo was investigated using an LPS-induced endotoxemia mouse model, demonstrating its dual activities: antibacterial activity against gram-negative bacteria and immunosuppressive effect preventing LPS-induced endotoxemia. Collectively, these results confirmed the therapeutic potential of Pro9-3 against infection of gram-negative bacteria.

• Biomolecules & Therapeutics
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• 제29권5호
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• pp.506-518
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• 2021

The imprinted tumour suppressor NOEY2 is downregulated in various cancer types, including ovarian cancers. Recent data suggest that NOEY2 plays an essential role in regulating the cell cycle, angiogenesis and autophagy in tumorigenesis. However, its detailed molecular function and mechanisms in ovarian tumours remain unclear. In this report, we initially demonstrated the inhibitory effect of NOEY2 on tumour growth by utilising a xenograft tumour model. NOEY2 attenuated the cell growth approximately fourfold and significantly reduced tumour vascularity. NOEY2 inhibited the phosphorylation of the signalling components downstream of phosphatidylinositol-3'-kinase (PI3K), including phosphoinositide-dependent protein kinase 1 (PDK-1), tuberous sclerosis complex 2 (TSC-2) and p70 ribosomal protein S6 kinase (p70S6K), during ovarian tumour progression via direct binding to vascular endothelial growth factor receptor-2 (VEGFR-2). Particularly, the N-terminal domain of NOEY2 (NOEY2-N) had a potent anti-angiogenic activity and dramatically downregulated VEGF and hypoxia-inducible factor-1α (HIF-1α), key regulators of angiogenesis. Since no X-ray or nuclear magnetic resonance structures is available for NOEY2, we constructed the three-dimensional structure of this protein via molecular modelling methods, such as homology modelling and molecular dynamic simulations. Thereby, Lys15 and Arg16 appeared as key residues in the N-terminal domain. We also found that NOEY2-N acts as a potent inhibitor of tumorigenesis and angiogenesis. These findings provide convincing evidence that NOEY2-N regulates endothelial cell function and angiogenesis by interrupting the VEGFR-2/PDK-1/GSK-3β signal transduction and thus strongly suggest that NOEY2-N might serve as a novel anti-tumour and anti-angiogenic agent against many diseases, including ovarian cancer.

• 한국재료학회지
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• 제31권6호
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• pp.345-352
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• 2021

In this study, Ni nanoparticle supported by graphene oxide (GO) (Ni-GO) is successfully synthesized through hydrothermal synthesis and calcination, and Cr(VI) is extracted from aqueous solution. The morphology and structure of Ni-GO composites are characterized by scanning electron microscopy (SEM), trans mission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). High-resolution transmission electron microscopy (HRTEM) and XRD confirms the high dispersion of Ni nanoparticle after support by GO. Loading Ni on GO can obviously enhance the stability of Ni-GO composites. It can be calculated from TGA that the mass percentage of Ni is about 60.67 %. The effects of initial pH and reaction time on Cr(VI) removal ability of Ni-GO are investigated. The results indicate that the removal efficiency of Cr(VI) is greater than that of bared GO. Ni-GO shows fast removal capacity for Cr(VI) (<25 min) with high removal efficiency. Dynamic experiments show that the removal process conforms to the quasi-second order model of adsorption, which indicates that the rate control step of the removal process is chemical adsorption. The removal capacity increases with the increase of temperature, indicating that the reaction of Cr(VI) on Ni-GO composites is endothermic and spontaneous. Combined with tests and characterization, the mechanism of Cr(VI) removal by rapidly adsorption on the surface of Ni-GO and reduction by Ni nanoparticle is investigated. The above results show that Ni-GO can be used as a potential remediation agent for Cr(VI)-contaminated groundwater.

• 한국전자통신학회논문지
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• 제16권6호
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• pp.1153-1160
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• 2021

본 논문은 소프트웨어 정의 네트워크에서 심층강화학습을 활용하여 최적의 경로를 결정하는 라우팅 알고리즘을 제안한다. 학습을 위한 심층강화학습 모델은 DQN 을 기반으로 하고, 입력은 현재 네트워크 상태, 발신지, 목적지 노드이고, 출력은 발신지에서 목적지까지의 경로 리스트를 반환한다. 라우팅 작업을 이산 제어 문제로 정의하며, 라우팅을 위한 서비스 품질 파라미터는 지연, 대역폭, 손실률을 고려하였다. 라우팅 에이전트는 사용자의 서비스 품질 프로파일에 따라 적절한 서비스 등급으로 분류하고, SDN에서 수집된 현재 네트워크 상태로부터 각 링크 별로 제공할 수 있는 서비스 등급을 변환한다. 이러한 변환된 정보를 토대로 발신지에서부터 목적지까지 요구되는 서비스 등급을 만족시키는 경로를 선택하도록 학습을 한다. 시뮬레이션 결과는 제안한 알고리즘이 일정한 에피소드를 진행하게 되면 올바른 경로를 선택하게 되고, 학습이 성공적으로 수행됨을 나타냈다.

• Journal of Periodontal and Implant Science
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• 제52권5호
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• pp.383-393
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• 2022

Purpose: Aloe-emodin (AE), a natural anthraquinone abundant in aloe plants and rhubarb (Rheum rhabarbarum), has long been used to treat chronic inflammatory diseases. However, AE's underlying mechanisms in periodontal inflammation have not been fully elucidated. Acidic mammalian chitinase (AMCase) is a potential biomarker involved in bone remodeling. This study aimed to evaluate AE's effect on periodontitis in rats and investigate AMCase expression. Methods: Eighteen Sprague-Dawley rats were separated into the following groups: healthy (group 1), disease (group 2), vehicle (group 3), AE high-dose (group 4), and AE low-dose (group 5). Porphyromonas gingivalis ligatures were placed in rats (groups 2-5) for 7 days. Groups 4 and 5 were then treated with AE for an additional 14 days. Saliva was collected from all groups, and probing pocket depth was measured in succession. Periodontal pocket tissues were subjected to histomorphometric analysis after the rats were sacrificed. Bone marrow-derived macrophages and murine macrophages were stimulated with receptor activator of nuclear factor-κB ligand (RANKL) and treated with different concentrations of AE. AMCase expression was detected from the analysis of saliva, periodontal pocket tissues, and differentiated osteoclasts. Results: Among rats with P. gingivalis-induced periodontitis, the alveolar bone resorption levels and periodontal pocket depth were significantly reduced after treatment with AE. AMCase protein expression was significantly higher in the disease group than in the healthy control (P<0.05). However, AE inhibited periodontal inflammation by downregulating AMCase expression in saliva and periodontal pocket tissue. AE significantly reduced RANKL-stimulated osteoclastogenesis by modulating AMCase (P<0.05). Conclusions: AE decreases alveolar bone loss and periodontal inflammation, suggesting that this natural anthraquinone has potential value as a novel therapeutic agent against periodontal disease.