• Bulletin of the Korean Chemical Society
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• 제13권2호
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• pp.119-122
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• 1992

The affinity chromatographic technique was applied to the isolation of Thromboxane Synthase, with a variety of imidazolyl alkanoic acids coupled Sepharose 2B including a gel (G in Table 4) which has one free COOH group in the bound affinity ligand. The effect of ligand structure on the "affinity" and "selectivity" for thromboxane synthase isolation is described.

• 유통과학연구
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• 제15권4호
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• pp.15-23
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• 2017

Purpose - This research aims to determine whether the consumer affinity and ethnocentrism as well as the image of a foreign country (Japanese product as the most popular product in Indonesia) are able to influence behavior related to the perceived risk and willingness to buy foreign products from the affinity country. Research design, data, and methodology - Using survey techniques with 164 respondents, the study uses structural equation model with confirmatory factor analysis (CFA). To ensure the research objective and appropriate respondent, then we select an individual who have interest on Japanese culture & language. The primary and secondary data used in this study. Primary data refers to information collected directly from respondent by questionnaires dissemination while secondary data is provided from well-established literatures. Results - The results show us that the ethnocentrism has dominant affection role compared to affinity in order to influence consumer behavior meanwhile, the product country image has cognition role to evoke consumer desire to consume foreign products. Conclusions - From a theoretical perspective, the study contributes to international marketing literature by refining the conceptualization of the consumer affinity construct and highlighting its multidimensional nature. The consumer affinity research need to enrich in term of the context and the different culture and situation.

• BMB Reports
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• 제45권4호
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• pp.233-238
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• 2012

We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome.

• 한국신뢰성학회지:신뢰성응용연구
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• 제6권1호
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• pp.51-61
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• 2006

Failure analyses were conducted on a crank shaft and a chock liner by using X-ray diffraction, optical microscopy and SEM/EDS techniques. In the crank shaft, a crack developed where a maximum tensile stress coincided with band structure formed by hot forging. The maximum tensile stress was observed to originate from volume expansion during high frequency induction heat treatment and the band structure to develop between upper and lower dies during hot forging. In the chock liner, the wear mechanism varied with the chemical affinity and hardness of liner material relative to friction pair of housing liner. Brass of low chemical affinity and hardness compared to housing liner showed uniform adhesive wear. STS 304 and STS 420J2 of high chemical affinity showed galling and scoring respectively.

• 한국환경농학회지
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• 제25권4호
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• pp.371-381
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• 2006

New proteomic techniques have been pioneered extensively in recent years, enabling the high-throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes. protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which ale associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.196-204
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• 2010

With the completion of genome sequencing of several organisms, attention has been focused to determine the function and functional network of proteins by proteome analysis. The recent techniques of proteomics have been advanced quickly so that the high-throughput and systematic analyses of cellular proteins are enabled in combination with bioinformatics tools. Furthermore, the development of proteomic techniques helps to elucidate the functions of proteins under stress or diseased condition, resulting in the discovery of biomarkers responsible for the biological stimuli. Ultimate goal of proteomics orients toward the entire proteome of life, subcellular localization, biochemical activities, and their regulation. Comprehensive analysis strategies of proteomics can be classified as three categories: (i) protein separation by 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification by either Edman sequencing or mass spectrometry (MS), and (iii) quanitation of proteome. Currently MS-based proteomics turns shiftly from qualitative proteome analysis by 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, to quantitative proteome analysis. Some new techniques which include top-down mass spectrometry and tandem affinity purification have emerged. The in vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tag, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope labeled amino acid can be in vivo labeled into live culture cells through metabolic incorporation. MS-based proteomics extends to detect the phosphopeptide mapping of biologically crucial protein known as one of post-translational modification. These complementary proteomic techniques contribute to not only the understanding of basic biological function but also the application to the applied sciences for industry.

• 한국건설관리학회논문집
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• 제22권5호
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• pp.29-38
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• 2021

정부와 기업이 건설업의 산업재해를 줄이기 위해 지속적으로 노력하고 있지만, 재해는 크게 줄어들지 않고 있다. 본 연구는 건설 재해에 영향을 미치는 요인들 간의 연관성을 정량적으로 규명하고자 하였다. 산업안전공단에서 공개한 중대재해 사례 1,197건을 대상으로, 데이터마이닝 기법 중 하나인 연관성 분석을 이용하여 연구를 수행하였다. 산업안전공단에서 제공하는 데이터와 외부 변수를 포함하여 재해 발생 형태, 건설업종, 작업내용, 기인물, 체감온도, 사고 시간대, 추락높이의 변수로 아이템을 구성하여 분석하였으며, 떨어짐 재해와 그 외의 재해로 구분하여 연관규칙을 도출하였다. 떨어짐 재해의 경우 향상도가 1.38 이상인 64개의 연관규칙을 도출하였으며, 떨어짐을 제외한 재해의 경우 향상도가 1.54 이상인 59개의 연관규칙을 도출하였다. 도출된 연관규칙을 재해요인 간의 연관성에 초점을 두고 해석한 후, 고찰에서 연구의 한계와 건설재해 요인 간의 관련성을 파악할 때 연관성 분석 기법을 적용함에 있어 유의사항을 제시하였다. 본 연구는 건설 재해에 영향을 미치는 요인들 간의 연관성을 정량적인 수치로 제시하여 추후 근로자들과 현장관리자가 건설현장에서 적절한 안전대책을 마련하는 기초자료를 제공하였다는 점에서 의미를 찾을 수 있다.

• 약학회지
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• 제28권3호
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• pp.139-147
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• 1984

L-PHA, a lectin having lymphoagglutinating activity but devoid of erythroagglutinability which is contained in Korean white kidney bean (Phaseolus vulgaris L.), was isolated and purified through several techniques such as ion exchange chromatography, hydroxyapatite column and affinity chromatography. Purified L-PHA was identified as a single band by polyacrylamide disc gel electrophoresis. The molecular weight of the L-PHA was estimated about 125, 000 daltons by polyacrylamide gel electrophoresis and it was turned out having one subunit (probably dimer of the Yachnin's model) of Mr~60, 000. Immunochemical studies also tried and these results reconfirmed the purity of this L-PHA.

• 한국건설관리학회논문집
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• 제14권3호
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• pp.157-166
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• 2013

BTL(Build-Transfer-Lease) 사업의 목적은 종래 BTO(Build-Transfer-Operate) 방식과는 달리 학교, 사회복지시설 등 최종 이용자로부터 이용료를 부과할 수 없는 공공시설에 대하여 민간자금을 유치함으로써 정부의 재정부족을 해결하고 사회기반시설의 조기 확충을 위한 방안으로 도입되었다. 하지만 BTL 사업은 그 진행과정에서 다양한 문제점을 발생시키고 있으며, 이를 개선할 수 있는 실질적인 접근이 요구된다. 이에 본 연구에서는 국내의 BTL사업의 문제점에 대한 연구 자료와 국내보다 앞서 BTL 사업을 활용하고 있는 국외 자료를 분석하여 문제 영역을 도출하였다. 그리고 이를 체계적으로 관리하기 위해 친화도 기법, 매트릭스 비교분석, AHP 기법을 활용하여 중요도를 분석하였다. 분석된 결과는 BTL 사업의 실질적 문제 영역 및 중요도 제시를 통하여 관리 기준을 제공할 것으로 기대된다.

• 한국가시화정보학회지
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• 제20권3호
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• pp.136-145
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• 2022

Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 10¹² copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.