• 제목/요약/키워드: Affinity Model

검색결과 237건 처리시간 0.022초

호텔요리전공 특성화고등학생들의 사회적 학습 성취도가 요리전공만족도에 미치는 영향 (An effects on social maturity in satisfaction with the academic major of high school students majoring in culinary arts)

  • 박정준
    • 한국융합학회논문지
    • /
    • 제9권2호
    • /
    • pp.191-200
    • /
    • 2018
  • 본 연구는 요리전공 고등학생들의 요리전공에 선택적 속성과 사회적으로 성숙함의 영향관계는 통계적으로 유의한 관계를 나타났으며, 요리전공의 선택적 속성은 구성원관계 친화력에 영향을 미치며 요리전공 선택적 요인은 사회적으로 유대감과 자기 능력관리에 영향을 미치는 것으로 나타났으며 요리전공 결정 선택과정은 학교생활의 월등한 적응력에 영향을 미치는 것으로 나타났다. 본 연구는 요리전공 고등학교 학생들의 사회적 성숙과 전공에 대한 만족도의 영향관계는 모델의 가설에 통계적으로 유의한 것으로 나타났으며, 구성원 관계적 친화력과 사회적 친밀감은 개설교과과정 모델참여에 영향을 미치는 것으로 나타났으며, 사회적 성숙도는 사회적 친밀감에 영향을 미치는 것으로 나타났다.

Elucidation of the Inhibitory Effect of Phytochemicals with Kir6.2 Wild-Type and Mutant Models Associated in Type-1 Diabetes through Molecular Docking Approach

  • Jagadeb, Manaswini;Konkimalla, V. Badireenath;Rath, Surya Narayan;Das, Rohit Pritam
    • Genomics & Informatics
    • /
    • 제12권4호
    • /
    • pp.283-288
    • /
    • 2014
  • Among all serious diseases globally, diabetes (type 1 and type 2) still poses a major challenge to the world population. Several target proteins have been identified, and the etiology causing diabetes has been reasonably well studied. But, there is still a gap in deciding on the choice of a drug, especially when the target is mutated. Mutations in the KCNJ11 gene, encoding the kir6.2 channel, are reported to be associated with congenital hyperinsulinism, having a major impact in causing type 1 diabetes, and due to the lack of its 3D structure, an attempt has been made to predict the structure of kir6.2, applying fold recognition methods. The current work is intended to investigate the affinity of four phytochemicals namely, curcumin (Curcuma longa), genistein (Genista tinctoria), piperine (Piper nigrum), and pterostilbene (Vitis vinifera) in a normal as well as in a mutant kir6.2 model by adopting a molecular docking methodology. The phytochemicals were docked in both wild and mutated kir6.2 models in two rounds: blind docking followed by ATP-binding pocket-specific docking. From the binding pockets, the common interacting amino acid residues participating strongly within the binding pocket were identified and compared. From the study, we conclude that these phytochemicals have strong affinity in both the normal and mutant kir6.2 model. This work would be helpful for further study of the phytochemicals above for the treatment of type 1 diabetes by targeting the kir6.2 channel.

각기 다른 단백질섭취 수준에서 본 식이단백질의 질이 단백질대사에 미치는 영향 -Simulation Model을 이용하여- (Simulation of the Effect of Protein Quality at the Different Protein Intake Level on Protein Metabolism)

  • 이옥희
    • Journal of Nutrition and Health
    • /
    • 제26권9호
    • /
    • pp.1033-1048
    • /
    • 1993
  • This study was designed to describe the effect of the protein quality at different intake level of protein on the protein metabolism in the whole body of growing pigs with a simulation model. Varying to the protein level in feeds, four simulations were conducted. The feed protein level, represented as proportions of digestible protein to the metabolic energy (DP/ME, g/MJ), were 6-8, 11-13, 17-19, and 23-25 DP/ME, respectively. Two protein quality and six weeks of growth time were used at each simulation. The objective function for the simulations was protein deposition in the whole body, which was calculated from the experimental results. The parameters in the simulation were determined by the parameter estimation technique. The results obtained from the simulation were as follows: The protein synthesis and breakdown rates(g/day) in the whole body was increased with the increase of protein quality only at lower or required level of protein intake. They showed a parallel behavior in the course of growth, irrespective of quality and level of feed protein intake. The simulated protein deposition and protein synthesis showed a linear relationship between them at different protein quality and level. The affinity parameter showed a linear relationship between them at different protein quality and level. The affinity parameter showed that arginine, tryptophan and isoleucine were more efficient in the stimulation ofbody protein synthesis. Lysine and phenylalanine+tyrosine were less efficient. The oxidation parameter showed that histidine, pheyalanine+tyrosine were less efficient. The oxidation parameter showed that histidine, phenyalanine+tyrosine, and methionine+cystine were oxidized in larger magnitude than lysine and threonine. The oxidation parameter of most amino acids increased with the increase of protein intake beyond the requirement level, but not any more at highest protein intake level. Finally it was found that the improvement of feed protein quality at the lower or required level of protein intake increase protein deposition through a parallel increase of protein synthesis and breakdown.

  • PDF

Role of High-affinity Choline Transporter 1 in Colonic Hypermotility in a Rat Model of Irritable Bowel Syndrome

  • Lin, Meng-juan;Yu, Bao-ping
    • Journal of Neurogastroenterology and Motility
    • /
    • 제24권4호
    • /
    • pp.643-655
    • /
    • 2018
  • Background/Aims Irritable bowel syndrome (IBS) is a common disease characterized by intestinal dysmotility, the mechanism of which remains elusive. We aim to determine whether the high-affinity choline transporter 1 (CHT1), a determinant of cholinergic signaling capacity, modulates intestinal motility associated with stress-induced IBS. Methods A rat IBS model was established using chronic water avoidance stress (WAS). Colonic pathological alterations were evaluated histologically and intestinal motility was assessed by intestinal transit time and fecal water content (FWC). Visceral sensitivity was determined by visceromotor response to colorectal distension. RT-PCR, western blotting, and immunostaining were performed to identify colonic CHT1 expression. Contractility of colonic muscle strips was measured using isometric transducers. enzyme-linked immunosorbent assay was used to measure acetylcholine (ACh). We examined the effects of MKC-231, a choline uptake enhancer, on colonic motility. Results After 10 days of WAS, intestinal transit time was decreased and fecal water content increased. Visceromotor response magnitude in WAS rats in response to colorectal distension was significantly enhanced. Protein and mRNA CHT1 levels in the colon were markedly elevated after WAS. The density of CHT1-positive intramuscular interstitial cells of Cajal and myenteric plexus neurons in WAS rats was higher than in controls. Ammonium pyrrolidine dithiocarbamate partly reversed CHT1 upregulation and alleviated colonic hypermotility in WAS rats. Pharmacological enhancement of CHT1 activity by MKC-231 enhanced colonic motility in control rats via upregulation of CHT1 and elevation of ACh production. Conclusion Upregulation of CHT1 in intramuscular interstitial cells of Cajal and myenteric plexus neurons is implicated in chronic stress-induced colonic hypermotility by modulation of ACh synthesis via nuclear factor-kappa B signaling.

노로바이러스 검출을 위한 측면유동면역분석법 기반의 바이오리셉터 선별기법 개발 (Norovirus Targeted Bioreceptor Screening Method based on Lateral Flow Immunoassay (LFIA))

  • 장희수;조현지;전태준;김선민
    • 한국가시화정보학회지
    • /
    • 제20권3호
    • /
    • pp.136-145
    • /
    • 2022
  • Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 1012 copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.

LISREL 구조방정식 모델에 의한 농촌 관광농원의 환경친화성 평가 모형 추정 (Evaluation Model for Environmentally Friendliness of Tourism Farms by LISREL Structural Equation Model)

  • 엄붕훈
    • 농촌계획
    • /
    • 제5권2호
    • /
    • pp.56-65
    • /
    • 1999
  • Recently, new concept and paradigm of 'Environmentally-Friendliness' is taking a growing interest in environmental planning and design. This study is to establish the evaluation model for environmentally-friendliness of 'Tourism Farms' in rural areas by LISREL structural equation model. A questionnaire survey was conducted for deputy manager group and expert group. As the Result of LISREL structural equation model, the environmentally-friendliness of tourism farms is composed of three categories. First, conservation of global environment (Low Impact), second, friendliness to surrounding nature(High Contact), and third, environmental health and amenities (Health & Amenity). Five indicators, such as (1)saving of energy and water resource, (2)reduction and reuse of garbage, (3)natural purification of sewage disposal, (4)utilization of natural energy, (5)campaign and education programs of environmentally-friendliness, were affecting the first category, i.e., conservation of global environment(Low Impact). Friendliness to surrounding nature (High Contact) is affected by 3 indicators, (1)contact to nature and diverse green areas, (2)water intimate & contact areas, (3)natural ecology observation by biotope. Finally, the dimension of environmental health and amenity is affected by 3 indicators, (1)nature affinity by farming experience, (2)environmental-friendliness of soil & crops by organic farming, (3) campaign and education programs of environmentally-friendliness. Total coefficient of determination of the structural equation model by LISREL was 0.897, which showed high explanatory power.

  • PDF

Glass Dissolution Rates From MCC-1 and Flow-Through Tests

  • Jeong, Seung-Young
    • 한국방사성폐기물학회:학술대회논문집
    • /
    • 한국방사성폐기물학회 2004년도 학술논문집
    • /
    • pp.257-258
    • /
    • 2004
  • The dose from radionuclides released from high-level radioactive waste (HLW) glasses as they corrode must be taken into account when assessing the performance of a disposal system. In the performance assessment (PA) calculations conducted for the proposed Yucca Mountain, Nevada, disposal system, the release of radionuclides is conservatively assumed to occur at the same rate the glass matrix dissolves. A simple model was developed to calculate the glass dissolution rate of HLW glasses in these PA calculations [1]. For the PA calculations that were conducted for Site Recommendation, it was necessary to identify ranges of parameter values that bounded the dissolution rates of the wide range of HLW glass compositions that will be disposed. The values and ranges of the model parameters for the pH and temperature dependencies were extracted from the results of SPFT, static leach tests, and Soxhlet tests available in the literature. Static leach tests were conducted with a range of glass compositions to measure values for the glass composition parameter. The glass dissolution rate depends on temperature, pH, and the compositions of the glass and solution, The dissolution rate is calculated using Eq. 1: $rate{\;}={\;}k_{o}10^{(ph){\eta})}{\cdot}e^{(-Ea/RT)}{\cdot}(1-Q/K){\;}+{\;}k_{long}$ where $k_{0},\;{\eta}$ and Eaare the parameters for glass composition, pH, $\eta$ and temperature dependence, respectively, and R is the gas constant. The term (1-Q/K) is the affinity term, where Q is the ion activity product of the solution and K is the pseudo-equilibrium constant for the glass. Values of the parameters $k_{0},\;{\eta}\;and\;E_{a}$ are the parameters for glass composition, pH, and temperature dependence, respectively, and R is the gas constant. The term (1-Q/C) is the affinity term, where Q is the ion activity product of the solution and K is the pseudo-equilibrium constant for the glass. Values of the parameters $k_0$, and Ea are determined under test conditions where the value of Q is maintained near zero, so that the value of the affinity term remains near 1. The dissolution rate under conditions in which the value of the affinity term is near 1 is referred to as the forward rate. This is the highest dissolution rate that can occur at a particular pH and temperature. The value of the parameter K is determined from experiments in which the value of the ion activity product approaches the value of K. This results in a decrease in the value of the affinity term and the dissolution rate. The highly dilute solutions required to measure the forward rate and extract values for $k_0$, $\eta$, and Ea can be maintained by conducting dynamic tests in which the test solution is removed from the reaction cell and replaced with fresh solution. In the single-pass flow-through (PFT) test method, this is done by continuously pumping the test solution through the reaction cell. Alternatively, static tests can be conducted with sufficient solution volume that the solution concentrations of dissolved glass components do not increase significantly during the test. Both the SPFT and static tests can ve conducted for a wide range of pH values and temperatures. Both static and SPFt tests have short-comings. the SPFT test requires analysis of several solutions (typically 6-10) at each of several flow rates to determine the glass dissolution rate at each pH and temperature. As will be shown, the rate measured in an SPFt test depends on the solution flow rate. The solutions in static tests will eventually become concentrated enough to affect the dissolution rate. In both the SPFt and static test methods. a compromise is required between the need to minimize the effects of dissolved components on the dissolution rate and the need to attain solution concentrations that are high enough to analyze. In the paper, we compare the results of static leach tests and SPFT tests conducted with simple 5-component glass to confirm the equivalence of SPFT tests and static tests conducted with pH buffer solutions. Tests were conducted over the range pH values that are most relevant for waste glass disssolution in a disposal system. The glass and temperature used in the tests were selected to allow direct comparison with SPFT tests conducted previously. The ability to measure parameter values with more than one test method and an understanding of how the rate measured in each test is affected by various test parameters provides added confidence to the measured values. The dissolution rate of a simple 5-component glass was measured at pH values of 6.2, 8.3, and 9.6 and $70^{\circ}C$ using static tests and single-pass flow-through (SPFT) tests. Similar rates were measured with the two methods. However, the measured rates are about 10X higher than the rates measured previously for a glass having the same composition using an SPFT test method. Differences are attributed to effects of the solution flow rate on the glass dissolution reate and how the specific surface area of crushed glass is estimated. This comparison indicates the need to standardize the SPFT test procedure.

  • PDF

의류제품의 우회광고가 소비자의 구매행동에 미치는 영향 -TV드라마 의상협찬을 중심으로- (The Effect of Indirect Advertisement to Consumers' Clothing Purchase Behavior -Focusing on Clothing Sponsorship of Television Drama-)

  • 이은정;이은영
    • 복식
    • /
    • 제52권4호
    • /
    • pp.141-154
    • /
    • 2002
  • The purpose of this study was to define 'sponsorship' as a kind of advertising tool, and to describe its effects on consumer brand attitude and actual purchase behavior. From previous research results, clothing sponsorship was defined as 'indirect advertisement', which has common ad traits as well as the uncommon ad traits, such as indirectness, emotional affinity, and symbolism. Results from empirical research using 1,227 data collected in Seoul and Kyounggi Areas were as follows ; (1) Television drama clothing sponsorship was proved to have effects on brand image, emotional & rational brand attitudes, decision making process, and actual purchase behavior. (2) Each of drama clothing sponsorship factors, such as 'drama', 'role', and 'actress', had different effects on brand memory and attitudes. (3) Consumer decision making process initiated by drama clothing sponsorship followed two different processes, which were called 'rational' and 'heuristic'. The rational process followed every steps of EKB model, which explained consumer's rational shopping, but heuristic model was closer to emotional and impulsive shopping.

Pose Estimation with Binarized Multi-Scale Module

  • Choi, Yong-Gyun;Lee, Sukho
    • International journal of advanced smart convergence
    • /
    • 제7권2호
    • /
    • pp.95-100
    • /
    • 2018
  • In this paper, we propose a binarized multi-scale module to accelerate the speed of the pose estimating deep neural network. Recently, deep learning is also used for fine-tuned tasks such as pose estimation. One of the best performing pose estimation methods is based on the usage of two neural networks where one computes the heat maps of the body parts and the other computes the part affinity fields between the body parts. However, the convolution filtering with a large kernel filter takes much time in this model. To accelerate the speed in this model, we propose to change the large kernel filters with binarized multi-scale modules. The large receptive field is captured by the multi-scale structure which also prevents the dropdown of the accuracy in the binarized module. The computation cost and number of parameters becomes small which results in increased speed performance.

Classification and Regression Tree Analysis for Molecular Descriptor Selection and Binding Affinities Prediction of Imidazobenzodiazepines in Quantitative Structure-Activity Relationship Studies

  • Atabati, Morteza;Zarei, Kobra;Abdinasab, Esmaeil
    • Bulletin of the Korean Chemical Society
    • /
    • 제30권11호
    • /
    • pp.2717-2722
    • /
    • 2009
  • The use of the classification and regression tree (CART) methodology was studied in a quantitative structure-activity relationship (QSAR) context on a data set consisting of the binding affinities of 39 imidazobenzodiazepines for the α1 benzodiazepine receptor. The 3-D structures of these compounds were optimized using HyperChem software with semiempirical AM1 optimization method. After optimization a set of 1481 zero-to three-dimentional descriptors was calculated for each molecule in the data set. The response (dependent variable) in the tree model consisted of the binding affinities of drugs. Three descriptors (two topological and one 3D-Morse descriptors) were applied in the final tree structure to describe the binding affinities. The mean relative error percent for the data set is 3.20%, compared with a previous model with mean relative error percent of 6.63%. To evaluate the predictive power of CART cross validation method was also performed.