• Title/Summary/Keyword: Aerobic

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Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage

  • Wang, Huili;Hao, Wei;Ning, Tingting;Zheng, Mingli;Xu, Chuncheng
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.2
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    • pp.198-207
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    • 2018
  • Objective: This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods: Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results: In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion: The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic deterioration of TMR silages.

Efficient aerobic denitrification in the treatment of leather industry wastewater containing high nitrogen concentration

  • Kang, Kyeong Hwan;Lee, Geon;Kim, Joong Kyun
    • Environmental Engineering Research
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    • v.20 no.1
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    • pp.79-87
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    • 2015
  • To treat leather industry wastewater (LIW) containing high nitrogen concentration, eight aerobic denitrifiers were isolated from sludge existing in an LIW-treatment aeration tank. Among them, one strain named as KH8 had showed the great ability in denitrification under an aerobic condition, and it was identified as Pseudomonas aeruginosa R12. The aerobic denitrification ability of the strain KH8 was almost comparable to its anaerobic denitrification ability. In lab-scale aerobic denitrifications performed in 1-L five-neck flasks for 48 hr, denitrification efficiency was found to be much improved as the strain KH8 held a great majority in the seeded cells. From the nitrogen balance at the cell-combination ratio of 10:1 (the strain KH8 to the other seven isolates) within the seeded cells, the percentage of nitrogen loss during the aerobic denitrification process was estimated to be 58.4, which was presumed to be converted to $N_2$ gas. When these seeded cells with lactose were applied to plant-scale aeration tank for 56 day to treat high-strength nitrogen in LIW, the removal efficiencies of $COD_{Cr}$ and TN were achieved to be 97.0% and 89.8%, respectively. Under this treatment, the final water quality of the effluent leaving the treatment plant was good enough to meet the water-quality standards. Consequently, the isolated aerobic denitrifiers could be suitable for the additional requirement of nitrogen removal in a limited aeration-tank capacity. To the best of our knowledge, this is the first report of aerobic denitrifiers applied to plant-scale LIW treatment.

Global Regulation of Gene Expression in the Human Gastric Pathogen Helicobacter pylori in Response to Aerobic Oxygen Tension Under a High Carbon Dioxide Level

  • Park, Shin Ae;Lee, Na Gyong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.451-458
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    • 2013
  • The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

Effect of Aerobic Exercise on Cognitive Functions in Childen and Adolescents : A Review (유산소 운동이 소아 및 청소년의 인지기능에 미치는 영향 : 문헌고찰)

  • Kang, Kyoung Doo;Cho, Jung Hwan;Han, Doug Hyun
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.26 no.3
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    • pp.143-148
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    • 2015
  • The purpose of this review was to investigate the relationship between aerobic exercise and cognitive function as well as synthesize the effect of aerobic exercise intervention studies centered on psychiatric symptoms associated with general cognitive deficit. Prospective studies on aerobic exercise and improvement of cognitive function were reviewed and synthesized. In addition, this literature review provides significant positive results on the relationship between aerobic exercise and general cognitive deficit associated with psychiatric symptoms. Review of this literature suggests that there is a positive relationship between participation in aerobic exercise and cognitive function. In this text, there are at least three general pathways by which aerobic exercise may facilitate executive function in children : 1) cognitive demands inherent in engaging in physical activity, 2) cognitive demands inherent in cooperation of complex motor tasks, and 3) physiological changes resulting from aerobic exercise. Another main finding of this review is that physical activity has a stronger influence on cognitive deficit, including attention-deficit hyperactivity disorder, depression, and anxiety. Development of cognitive function is the most important factor for children and youth. Therefore, future research should prove relationship between physical activity and cognitive function using a more scientific and quantitative approach design.

Comparison of the Effects of Squat Exercise, Bracing Exercise, and Aerobic Exercise on Lung Function (스쿼트 운동, 브레이싱 운동, 그리고 유산소 운동이 폐기능에 미치는 영향 비교)

  • Kim, Hyeon-Su;Kim, Chung-Yoo;Lee, Keon-Cheol
    • Journal of The Korean Society of Integrative Medicine
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    • v.10 no.2
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    • pp.169-176
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    • 2022
  • Purpose : The purpose of this study is to investigate the effect of squat, bracing and aerobic exercise on lung function, which is known to be effective for strength training, on lung function. Methods : The study was conducted with 33 students from Busan K university. Eleven students were assigned to squats, bracing, and aerobic exercise, six weeks three times a week. In order to measure lung activity, pony Fx manufactured the change amount of FVC (forced vital capacity), FEV1 (Forced expiratory volume at one second), and FEV1/FVC % (forced vital capacity/forced expiratory volume at one second) was analyzed after inputting the information of experimental group A and B controls. As a method of measurement, the difference between the three groups was analyzed using repeated ANOVA. Results : As a result of analyzing the effects of squat, bracing, and aerobic exercise for 6 weeks, all values of FVC, FEV1, FEV1/FVC % were increased from 0 weeks to 6 weeks except FEV1/FVC %. There was no significant difference in FVC from week 3 to week 6. In the squat, bracing, and aerobic exercise, the changes in spirometry showed that the FVC, FEV1, and FEV1/FVC % values in bracing exercise were significantly increased with time than before exercise. As a result of analyzing the changes in the spirometry of squat, bracing, and aerobic exercise, the FVC, FEV1, FEV1/FVC % values in the squat exercise showed statistically significant difference according to the period, but the lowest increase among the three groups. Conclusion : In conclusion, aerobic, bracing and squat exercises all had a significant impact on improving lung function. Therefore, even without aerobic exercise, squat or bracing exercise alone can be expected to improve lung function.

Comparison of Obesity Management Between a Behavior Modification Program and an Aerobic Exercise Program in Overweight Adolescent Girls (행동수정프로그램과 에어로빅 운동의 과체중 여자 중학생 비만관리 효과 비교)

  • 문정순;정승교
    • Journal of Korean Academy of Nursing
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    • v.26 no.4
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    • pp.799-807
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    • 1996
  • This study was done to compare the effects of managing obesity using a behavior modification program and an aerobic exercise in adolescent girls. Thirty three subjects were selected from one girls' middle school located in Seoul. Seventeen girls participated in the behavior modification program by joining 60-90 minute group sessions weekly. The aerobic exercise program was given to 16 girls three times per week for 60 minutes each. These iterventions continued for eight weeks from October 17 to December 10, 1995. The data were obtained before and after the interventions by measuring degree of obesity, body weight, blood pressure, serum lipid, %body fat, and waist to hip ratio(WHR). Data ware analysed by using paired t-test. The results are as follows. 1. Subjects in both the behavior modification and the aerobic exercise groups showed significant decrese in the degree of obesity, body weight, diastolic blood pressure, total cholesterol, low density lipoprotein, %body fat and fat mass after the 8-week interventions. 2. Systolic bood pressure, high density lipoprotein cholesterol and waist to hip ratio were significantly decreased after the 8-week intervention in the behavior modification program group while triglyceride was significantly decreased after the 8-week intervention in the aerobic exercise program group. These findings indicate that both behavior modification and aerobic exercise programs are good strategies for managing obesity among adolescent girls.

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The Effects of Aerobic Exercise Therapy on Physical Functions in the Elderly (에어로빅 운동 프로그램이 노인의 신체적 기능에 미치는 효과)

  • Jung, Sook-Hee;Chung, Kyung-Hee
    • Research in Community and Public Health Nursing
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    • v.21 no.2
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    • pp.252-262
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    • 2010
  • Purpose: Designed to examine the effects of aerobic exercise therapy on elders' physical functions. Methods: Selected from an elderly welfare center in an agricultural district located in N City. Thirty seven elders were selected in the experimental group and 38 in the control group, and all the subjects aged over 65. Collected data were statistically analyzed by SPSS/PC 12.0 Win. Detailed data analysis methods were Chi-square, Fisher's exact test, Kolmogorov-Smirnov test, t-test, Mann-Whitney u-test, paired t-test, and Wilcoxon's rank sum test. Results: The first hypothesis "The experimental group who had the aerobic exercise therapy will have greater development in lower leg muscular strength compared to the control group" was supported (t=8.95, p<.001). The second hypothesis "Aerobic exercise therapy participants will show greater development in lower leg endurance" was supported (t=6.12, p<.001). The third hypothesis "Aerobic exercise therapy participants will show greater development in flexibility" was supported (U=49.00, p<.001). The forth hypothesis "Aerobic exercise therapy participants will show greater development in balance" was supported (U=322.00, p<.001). Conclusion: The aerobic exercise therapy showed positive effects on physical functions of the elderly in a rural area.

Metabolic Flux Shift of Weissella kimchii sk10 Grown Under Aerobic Conditions

  • Park, Sun-Mi;Kang, Hye-Sun;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.919-923
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    • 2004
  • The sk10 isolated from kimchi was identified as W. kimchii on the basis of l6s-rDNA sequencing. Studies were made to analyze the metabolic flux shift of the sk10 on glucose under aerobic growth conditions. The sk10 produced 38.2 mM acetate, 16.3 mM ethanol, and 33.2 mM lactate under aerobic conditions, but 2.4 mM acetate, 48.0 mM ethanol, and 44.1 mM lactate under anaerobic conditions. The NADH peroxidase (NADH-dependent hydrogen peroxidase) activity of sk10 grown under aerobic conditions was 11 times higher than that under anaerobic conditions. Under the low ratio of $NADH/NAD^+$, the metabolic flux toward lactate and ethanol was shifted to the flux through acetate kinase without NADH oxidation. The kinds of enzymes and metabolites of sk10 were close to those in the pathway of Leuconostoc sp., but the metabolites produced under aerobic growth conditions were different from those of Leuconostoc sp. The stoichiometric balance calculated using the concentrations of metabolites and substrate was about 97%, coincident with the theoretical values under both aerobic and anaerobic conditions. From these results, it was concluded that the metabolic flux of W. kimchii sk10 was partially shifted from lactate and ethanol to acetate under aerobic conditions only.

Numerical Model for SBR Aerobic Digestion Combined with Ultrasonication and Parameter Calibration (초음파 결합형 SBR 호기성 소화의 모델과 매개변수의 보정)

  • Kim, Sunghong;Lee, Inho;Yun, Jeongwon;Lee, Dongwoo
    • Journal of Korean Society of Water and Wastewater
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    • v.27 no.4
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    • pp.457-468
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    • 2013
  • Based on the activated sludge model(ASM), a mathematical model which represents the aerobic sludge digestion by sequencing batch reactor(SBR) combined with ultrasonic treatment was composed and performed in this study. Aerobic digestion using sequencing batch reactor(SBR) equipped with ultrasound treatment was also experimented for the purpose of parameter calibration. Most of the presented kinetic parameters in ASM or ASM2 could be used for the aerobic digestion of sludge but the parameters related in hydrolysis and decay rate needed modification. Hydrolysis rate constant of organic matter in aerobic condition was estimated at $0.3day^{-1}$ and the maximum growth rate for autotrophs in aerobic condition was $0.618day^{-1}$. Solubilization reactions of particulate organics and nitrogen by ultrasonication was added in this kinetic model. The solubilization rate is considered to be proportional to the specific energy which is defined by specific ultrasound power and sonication time. The solubilization rate constant by ultrasonication was estimated at $0.202(W/L)^{-1}day^{-1}$ in this study. Autotrophs as well as heterotrophs also decomposed by ultrasonic treatment and the nitrification reaction was limited by the lack of autotrophs accumulation in the digester.

The Effect of Resistance Exercise on Aerobic Capacity for Old Adults and CAD Patients (저항운동이 CAD환자와 노인의 유산소 능력에 미치는 영향)

  • Yoon, Byung-Kon;Jin, Young-Wan;Kwak, Yi-Sub
    • Journal of Life Science
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    • v.18 no.11
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    • pp.1612-1616
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    • 2008
  • Aerobic capacity is an important health indicator which is related to the probability of disease, disability, and mortality. Typically, endurance exercise is known as the primary method of improving aerobic capacity. Although most of resistance exercises are not considered for a good method increasing aerobic capacity, low to moderate intensity resistance exercise with short rest periods may improve aerobic capacity, especially old adults and most low to moderate risk patients suffering from CAD. This review is to understand that a number of physiological changes occur during both aerobic and resistance exercise, and to support that resistance exercise has advantages for improving aerobic capacity.