Browse > Article
http://dx.doi.org/10.5713/ajas.17.0183

Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage  

Wang, Huili (College of Engineering, China Agricultural University)
Hao, Wei (College of Engineering, China Agricultural University)
Ning, Tingting (College of Engineering, China Agricultural University)
Zheng, Mingli (College of Engineering, China Agricultural University)
Xu, Chuncheng (College of Engineering, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.2, 2018 , pp. 198-207 More about this Journal
Abstract
Objective: This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods: Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results: In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion: The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic deterioration of TMR silages.
Keywords
Aerobic Stability; Deterioration; Silage; Total Mixed Ration; Yeast;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Owens V, Albrecht K, Muck R, Duke S. Protein degradation and fermentation characteristics of red clover and alfalfa silage harvested with varying levels of total nonstructural carbohydrates. Crop Sci 1999;39:1873-80.   DOI
2 Xu C, Cai Y, Moriya N, Ogawa M. Nutritive value for ruminants of green tea grounds as a replacement of brewers' grains in totally mixed ration silage. Anim Feed Sci Technol 2007;138:228-38.
3 Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Leeuwenhoek 1998;73:331-71.   DOI
4 Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673-80.   DOI
5 Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20.   DOI
6 Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
7 Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783-91.   DOI
8 Kurtzman C, Fell JW, Boekhout T. The yeasts: a taxonomic study. London, UK: Elsevier; 2011.
9 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9.1
10 Middelhoven WJ, van Baalen AH. Development of the yeast flora of whole-crop maize during ensiling and during subsequent aerobiosis. J Sci Food Agric 1988;42:199-207.   DOI
11 Makimura K, Murayama SY, Yamaguchi H. Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 1994;40:358-64.   DOI
12 McDonald P, Henderson A, Heron S. The biochemistry of silage. 2nd ed. Welton, Lincoln, UK: Chalcombe Publications; 1991.
13 Jonsson A, Pahlow G. Systematic classification and biochemical characterization of yeasts growing in grass silage inoculated with Lactobacillus cultures. Anim Res Dev 1984;20:7-22.
14 Kalathenos P, Sutherland J, Roberts T. Resistance of some wine spoilage yeasts to combinations of ethanol and acids present in wine. J Appl Bacteriol 1995;78:245-50.   DOI
15 Merico A, Sulo P, Piskur J, Compagno C. Fermentative lifestyle in yeasts belonging to the Saccharomyces complex. FEBS J 2007;274:976-89.   DOI
16 Li Y, Nishino N. Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage. Grassl Sci 2011;57:184-91.   DOI
17 Dolci P, Tabacco E, Cocolin L, Borreani G. Microbial dynamics during aerobic exposure of corn silage stored under oxygen barrier or polyethylene films. Appl Environ Microbiol 2011;77:7499-507.   DOI
18 Li Y, Nishino N. Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri. Lett Appl Microbiol 2011;52:314-21.   DOI
19 Carvalho B, Avila C, Miguel M, et al. Aerobic stability of sugar-cane silage inoculated with tropical strains of lactic acid bacteria. Grass Forage Sci 2015;70:308-23.
20 Sousa MJ, Rodrigues F, Coorte-Real M, Leao C. Mechanisms underlying the transport and intracellular metabolism of acetic acid in the presence of glucose in the yeast Zygosaccharomyces bailii. Microbiol 1998;144:665-70.   DOI
21 Pitt JI, Hocking AD, Diane A. Fungi and food spoilage. 3rd ed. New York, USA: Springer; 2009.
22 Campbell I. Beer. In: Doyle MP, Beuchat LR, editors. Food microbiology: fundamentals and frontiers. 3rd ed. Washington, DC, USA: ASM Press; 2007. p. 851-62.
23 Strom K. Fungal inhibitory lactic acid bacteria. Uppsala, Sweden: Swedish University of Agricultural Sciences; 2005.
24 Wang H, Ning T, Hao W, Zheng M, Xu C. Dynamics associated with prolonged ensiling and aerobic deterioration of total mixed ration silage containing whole crop corn. Asian-Australas J Anim Sci 2016;29:62-72.
25 Driehuis F, Elferink SO. The impact of the quality of silage on animal health and food safety: a review. Vet Q 2000;22:212-6.   DOI
26 Woolford MK. The detrimental effects of air on silage. J Appl Bacteriol 1990;68:101-16.   DOI
27 Courtin MG, Spoelstra SF. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass Forage Sci 1990;45:153-65.   DOI
28 Pahlow G, Muck RE, Driehuis F, Elferink SJ, Spoelstra SF. Microbiology of ensiling. In: Buxton DR, Muck RE, Harrison JH, editors. Silage science and technology. Madison, WI, USA: American Society of Agronomy; 2003. p. 31-93.
29 Hu X, Hao W, Wang H, et al. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian-Australas J Anim Sci 2015;28:502-10.
30 Tabacco E, Piano S, Revello-Chion A, Borreani G. Effect of Lactobacillus buchneri LN4637 and Lactobacillus buchneri LN40177 on the aerobic stability, fermentation products, and microbial populations of corn silage under farm conditions. J Dairy Sci 2011;94:5589-98.   DOI
31 Hao W, Wang H, Ning T, Yang F, Xu C. Aerobic stability and effects of yeasts during deterioration of non-fermented and fermented total mixed ration with different moisture levels. Asian-Australas J Anim Sci 2015;28:816-26.   DOI
32 AOAC. Offcial Methods of Analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists; 1990.