• Title/Summary/Keyword: Aeration Time Ratio

Search Result 54, Processing Time 0.036 seconds

Culture Condition of Pseudomonas aeruginosa F722 for Biosurfactant Production

  • Oh, Kyung-Taek;Kang, Chang-Min;Kubo, Motoki;Chung, Seon-Yong
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.11 no.6
    • /
    • pp.471-476
    • /
    • 2006
  • Pseudomonas aeruginosa F722 produces a biosurfactant (BS) during its degradation of carbon and hydrocarbon compounds. The culture conditions for upgrading the biosurfactant productivity were investigated. The concentration of the biosurfactant produced by P. aeruginosa F722 was 0.78 g/L in C-medium; however, this increased to 1.66 g/L in BS medium, which was experimentally adjusted to optimal conditions. $NaNO_{2}$ was found to be most effective for microbial growth, with an $O.D_{600nm}$ of 1.18 for 0.1 % $NaNO_{2}$. Microbial growths, according to the $O.D_{600nm}$ were 2.53, 2.68, 2.89, and 2.87 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Clear zone diameters (cm), indicating biosurfactant activity, were 9.0, 8.8, 5.7, and 8.5 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Microbial growth was not consistent with the biosurfactant activity. The best biosurfactant activity was found with a C/N ratio of 20. Under optimal culture condition, the average surface tension decreased from 70 to 30 mN/m after 5 days. With aeration of 1.0 vvm, the biosurfactant produced increased to 1.94 g/L (up to 20%) compared to that of 1.66 g/L with no aeration. With aeration, the velocities of glucose degradation during both the log and stationary growth phases increased from 0.25 and $0.18\;h^{-1}$ to 0.33 and $0.29\;h^{-1}$, respectively, and the time for the culture to arrive at the maximum clear zone diameter became shorter, from 80 down to 60 h with no aeration.

Inference of Sequencing Batch Reactor Process using Oxidation Reduction Potential (ORP profile을 이용한 연속 회분식 반응기(Sequencing Batch Reactor)에서 무산소공정 추론)

  • Sim, Mun Yong;Bu, Gyeong Min;Im, Jeong Hun;U, Hye Jin;Kim, Chang Won
    • Journal of Environmental Science International
    • /
    • v.13 no.3
    • /
    • pp.245-250
    • /
    • 2004
  • The SBR(Sequencing Batch Reactor) process is ideally suited to treat high loading wastewater due to its high dilution rate. SBR operates by a cycle of periods consisting of filling, reacting, settling, decanting and idling. The react phases such as aeration or non-aeration, organic oxidation, nitrification, denitrification and other bio-logical reactions can be achieved in a reactor. Although the whole reactions can be achieved in a SBR with time distributing, it is hard to manage the SBR as a normal condition without recognizing a present state. The present state can be observed with nutrient sensors such as ${NH_{4}}^{+}-N$, ${NO_{2}}^{-}-N$, ${NO_{3}}^{-}-N} and ${PO_{4}}^{ 3-}-P.$ However, there is still a disadvantage to use the nutrient sensors because of their high expense and inconvenience to manage. Therefore, it is very useful to use common on-line sensors such as DO, ORP and pH, which are less expensive and more convient. Moreover, the present states and unexpected changes of SBR might be predicted by using of them. This study was conducted to get basic materials for making an inference of SBR process from ORP(oxidation reduction potential) of synthetic wastewater. The profiles of ORP, DO, and pH were under normal nitrification and denitrification were obtained to compare abnormal condition. And also, nitrite and nitrate accumulation were investigated during reaction of SBR. The bending point on ORP profile was not entirely in the low COD/NOx ratio condition. In this case, NOx was not entirely removed, and minimum ORP value was presented over -300mV. Under suitable COD/NOx ratio which complete denitrification was achieved, ORP bending point was observed and minimum ORP value was under -300m V. Under high COD/NOx ratio, ORP bending point was not detected at the first subcycle because of the fast denitrification and minimum ORP value was under -300mV at the time.

STUDY ON THE EFFECT OF AERATED LUBRICANT ON THE JOURNAL TRACES IN THE ENGINE BEARING CLEARANCE

  • JANG S.;PARK Y.
    • International Journal of Automotive Technology
    • /
    • v.6 no.4
    • /
    • pp.421-427
    • /
    • 2005
  • This work analyzes the behaviors of aerated lubricant in the gap between con-rod bearing and journal. It is assumed that the film formation with aerated lubricant is influenced by the two major factors. One is the density characteristics of the lubricant due to the volume change of lubricant for the formation of bubbles and the other is the viscosity characteristics of the lubricant due to the surface tension of the bubble in the lubricant. These two major factors surprisingly increase the load capacity in some ranges of bubble sizes and densities. Modified Reynolds' equation is developed for the computation of fluid film pressure with the effects of aeration ratio in the lubricant. From the calculated load capacity by solving modified Reynolds' equation, journal locus is computed with Mobility method after comparing it with the applied load at each time step. The differences of journal orbits between aerated and pure lubricants are shown in the computed results.

Pilot-scale Study on Nitrogen Removal of Effluent from Biogas Plant (바이오가스 플랜트 처리수의 고농도 질소 제거)

  • Yoo, Sungin;Yu, Youngseob;Lee, Yongsei;Park, Hyunsu;Yoo, Heechan
    • 한국신재생에너지학회:학술대회논문집
    • /
    • 2011.11a
    • /
    • pp.175.1-175.1
    • /
    • 2011
  • A rotating activated bacillus contactor (RABC) process with a series of aerobic reactors was tested in pilot scale to treat digested liquid from an anaerobic digester treating swine wastewater and sewage sludge. The influent (digested liquid) for the RABC process showed C/N ratios less than 2 as a typical feature of effluent from anaerobic digesters. The pilot process, which consists of three 3 RABC reactors, four aerobic tanks and a sedimentation tank, was operated for 210 days with a hydraulic retention time of 20 days without pH and temperature control. Since the Bacillus-enriched aerobic reactors shows high efficiencies of nitrogen removal at low DO levels less than 1.0 mg/L, they were operated at reduced aeration intensities. With relatively low concentrations of organics in comparison with nitrogen concentrations, the RABC process tested in this study showed stable and high nitrogen and organics removal efficiencies over 80%. The nitrogen removal process tested in this study was proven to be an effective and operation-cost saving (lower aeration) method to remove nitrogen without adding external carbon sources to meet the optimum C/N ratio.

  • PDF

Removal Characteristic of Nitrogenous Compounds According to the Combination of Feeding Ratio between the Supernatant of Precipitation Tank and Raw Domestic Wastewater (침전조 상등액과 유입하수의 유량대비에 따른 하수 내 질소 화합물 제거특성)

  • Park, Sang Min;Park, Jin Hee
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.13 no.4
    • /
    • pp.128-135
    • /
    • 2005
  • This study was done to improve the effectiveness of nitrification and denitrification using the aeration-anoxic combination method using CFSTR(continuous-flow stirred-tank reactor) attached with an anoxic reactor filled with a media. In order to calculate the concentration of nitric acid within the aeration tank proportional to the anoxic rate within the reactor, supernatant within the inflow and precipitation tanks were influxed into the anoxic reactor. The rate of nitrogen removal was calculated using the concentration of inflow and flow of returned supernatant. From the results of this experiment, the carbon source needed in the anoxic reactor came from the inflow so that anoxification was achieved completely using the inflow source without the introduction of an external carbon source. However, as the ratio of nitric acid becomes large in inflow and nitric acid flow, the carbon source within the input source decreases so that the concentration of carbon source is important.

  • PDF

Comparison of Liquefying Efficiency of Mixed Organic Fertilizer as Affected by Aeration Time and the Ratio of Organic Fertilizer to Water (폭기시간과 유기질비료 농도에 따른 혼합유기질비료의 액비화 특성비교)

  • Lee, Jong-Tae;Ha, In-Jong;Moon, Jin-Seong;Song, Won-Doo
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.40 no.2
    • /
    • pp.156-163
    • /
    • 2007
  • This study was conducted to evaluate the liquefying efficiency of mixed organic fertilizer in different conditions. The organic fertilizer was composed of sesame oil cake, rice bran, fish meal, ground bone meal etc, and made by fermenting process. It included $23g\;kg^{-1}$, $17.0g\;kg^{-1}$, $23.9g\;kg^{-1}$, $290g\;kg^{-1}$ of N, $P_2O_5$, $K_2O$, organic matter, respectively. In one test, the mixed organic fertilizer was added in the proportion of 10% to water 90% and aerated continuously, for 2, 8 hours per day, and not aerated as control. In the other test, ratios of organic fertilizer to water were 5%, 10%, 20% and aerated for 2 hours a day. With the increase of liquefying time, pH, EC and $NH_4-N$ increased without relation to aeration time. After 10 days, liquid organic fertilizer aerated for 2 hours a day contained $634mg\;N\;kg^{-1}$, $68.1mg\;P_2O_5\;kg^{-1}$, $453mg\;K_2O\;kg^{-1}$, which was not significantly different from 8 hours a day or continuous aeration. Then extraction ratios of inorganic contents were 27.6%, 4.0% and 18.9%, respectively. Continuous aeration resulted in increasing the viable number of aerobic bacteria, spore forming bacteria and fungi in liquefied solution. Higher ratio of organic fertilizer to water increased EC, $NH_4-N$ and other inorganic matter contents, but decreased extraction ratio of nutrients in liquid fertilizer. The liquid organic fertilizer of 20% contained $1,140mg\;N\;kg^{-1}$, $35.4mg\;P_2O_5\;kg^{-1}$, $544mg\;K_2O\;kg^{-1}$ after 10 days. Then extraction ratios were 24.8%, 2.4% and 13.6%, respectively. The ratio of organic fertilizer to water was positively correlated with only spore forming bacteria, Pseudomonas spp. among microorganisms.

Nitrite Accumulation Characteristics According to Hydraulic Retention Time and Aeration Rate in a Biological Aerated Filter (생물여과 반응기에서 수리학적 체류시간 및 폭기량에 따른 아질산 축적 특성)

  • Yoon, Jong Moon;Kim, Dong Jin;Yoo, Ik-Keun
    • Korean Chemical Engineering Research
    • /
    • v.44 no.2
    • /
    • pp.200-206
    • /
    • 2006
  • In a biological aerated filter (BAF) packed with ceramic media (void fraction of BAF=0.32), nitrite accumulation was studied with the variation of hydraulic retention time (HRT) and superficial air velocity. Synthetic ammonium wastewater and petrochemical wastewater were fed at a constant load of $1.6kgNH_4^+-N/m^3{\cdot}d$. Ammonium removal rate was mainly affected by the superficial air velocity in BAF, but nitrite ratio($NO_2-N/NO_x-N$) in the effluent was dependent on both HRT and superficial air velocity. For a fixed HRT of 0.23 hr (corresponding to the empty bed contact time of 0.7 hr) ammonium removal rate was 73/90/92% and nitrite ratio was 0.92/0.82/0.48 at the superficial air velocity of 0.23/0.45/0.56 cm/s, respectively. When HRT is increased to 0.9 hr with superficial air velocity ranging from 0.34 to 0.45 cm/s, the ammonium removal rate was 89% on average. However nitrite ratio decreased significantly down to 0.13. When HRT was further increased to 1.4 hr, ammonium removal rate decreased, thereby resulting in the free ammonia ($NH_3-N$, FA) build-up and nitrite ratio gradually increased (>0.95). Although aeration rate and FA concentration at HRT of 0.23 hr were unfavorable for nitrite accumulation compared with those at HRT of 0.9 hr, nitrite ratio at HRT of 0.23 hr was higher. Taken together, HRT and nitrogen load were found to be critical, in addition to FA concentration and aeration condition, for nitrite accumulation in the BAF tested in the present study.

Effects of Chaff as Bulking Materials on Aerobic Composting of Food Wastes

  • Park, Seok-Hwan
    • Proceedings of the Korean Environmental Health Society Conference
    • /
    • 2003.06a
    • /
    • pp.208-212
    • /
    • 2003
  • This study was performed to examine the effects of chaff as bulking materials on temperature, pH, weight and volume reduction and salinity in aerobic composting of food wastes. Volume ratios of food wastes to chaff in reactor Control, Ch-1, Ch-2, Ch-3 and Ch-4 were 4:0, 4:1, 4:2, 4:3 and 4:4, respectively. Reactors were operated for 24 days with 1 hour stirring by 1 rpm and 2 hours aeration per day. The lowering of the volume ratio of food wastes to chaff resulted in the ascending of the highest reaction temperature and the elongation of the high temperature reaction period. The lowering of the volume ratio of food wastes to chaff resulted in the more fast time of pH ascending. The lowering of the volume ratio of food wastes to chaff resulted in the more fast consistency in the weight and volume reduction rates. Salinities were condensed by reaction days. The final salinity of Control was 2.79%, and the final range of salinities of chaff mixtures was 2.18 - 2.37%.

  • PDF

A Study on Removal Efficiency of T-N in Bench-scale for Shipboard Sewage Treatment Plant (Bench-scale 선박용 고도수처리장치에서의 T-N 제거효율 연구)

  • Choi, Young-Ik;Shin, Dae-Yeol;Lee, Seung-Chul;Jung, Jin-Hee;Yoon, Young-Nae
    • Journal of Environmental Science International
    • /
    • v.27 no.1
    • /
    • pp.39-45
    • /
    • 2018
  • In this study, the International Maritime Organization (IMO)'s guideline MEPC. 277 (64) was developed and evaluated for the removal efficiency of T-N in a SBR and MBR combined process. This combined process of resized equipment based on large capacity water treatment device for a protection of marine aquatic life. In this experiment, T-N concentration of influent and effluent was measured through with the artificial wastewater. The SBR reactor operation time was varied according to the C : N : P ratios so that different conditions for mixing and aeration period in mins (90 : 60, 80 : 40, 70 : 50) and two C: N: P ratios (10 : 5 : 3, 10 : 3 : 1) were used. During experiment in the reactor's aeration and anoxic tank DO concentrations were 3 mg/L and 0.2 mg/L respectively. Furthermore, in the reactor MLSS concentration was 2000 mg/L and flowrate was 2 L/hr. Experiment results showed that C : N : P, 10 : 3 : 1 ratio with 90 mins mixing and 60 mins aeration maximized removal efficiency at 97.3% T-N as compared to other conditions. The application of the SBR and MBR combined process showed efficient results.

Removal Character of Nitrogen and Phosphorus in Swine Wastewater with Injection Time of Acetic Acid on SBR (SBR에서 아세트산 주입시간변화에 따른 양돈폐수의 질소, 인 제거특성)

  • Huh, Mock;Lee, Yong-Doo;Kang, Jin-Young
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.11 no.1
    • /
    • pp.132-137
    • /
    • 2003
  • This study was carried out to investigate removal character of nitrogen and phosphorus with injection time of acetic acid on SBR, which is one of the biological treatment process. Wastewater used in experiment period was swine wastewater with character, relatively lower organic material concentration than nitrogen concentration. In the experiment with injection time of acetic acid, run 1 wasn't injected acetic acid during the anoxic period, and run 2 was injected intermittently acetic acid during the anoxic period of 15 hours. And run 3 was injected intermittently during the anoxic period of 3hours from end of wastewater filling. And filing time of the wastewater was 20hour from run 1 to run 3. In the study, the highest removal efficiency of organic and nitrogen were obtained by the operating condition of Run 2(the ratio of mixing/aeration time : 16.5/5.5, injection time of acetic acid : 15hours) and T-P was obtained by the operation condition of Run 3(the ratio of mixing/aeration time : 16.5/5.5, injection time of acetic acid : 3hours),and removal efficiency of $BOD_5$, $COD_{Mn}$, $COD_{Cr}$, T-N and T-P in the treated water was 96.1%, 87.7%, 90.6%, 86.6% and 84.5%, respectively.

  • PDF