• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.337-337
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• 2017

Accurate and optimal water supply to cereal crop is critical in growing stalks and producing maximum yields. Excessive soil moisture may cause nutrient deficiencies and oxygen deficiency. Excessive soil water during crop growth stages results in decrease of yields. In Korea, the largest agricultural lands are paddy fields. Recently, upland crops are cultivated in paddy field soils to reduce overproduced rice in Korea. In order to success this policy, it is necessary to fully understand crop response to excessive soil moisture condition from paddy field soils. Adzuki bean is one of major legumes which provide protein in daily diet. Adzuki bean has been well know its weakness to excessive soil moisture condition, In order to obtain optimal yields of adzuki bean from paddy field cultivation, it is necessary to understand response of adzuki bean under different soil moisture conditions. This study investigated characteristics of growths, yields and response degree of water stress from adzuki bean. Three cultivars were selected for this study; Chungju, Hongeon, and Arari. All adzuki beans were cultivated in a paddy field which was divided into three sections with different soil moistures. The paddy field was located in Milyang, Gyeongsangnam during 2016. One section of the paddy field had the greatest average soil moisture content as 35.1% during adzuki bean cultivation (very poor). The second greatest soil moisture section had 32.6% (somewhat poor) and the smallest soil moisture section had 28.9% of soil moisture (somewhat well). During cultivation of three cultivar adzuki beans, soil moisture contents and groundwater levels were monitored. All the characteristics of growth and yield components were measured; height, thickness, 100 seed weights etc. Stress index values were calculated by Stress Day Index (SDI). All cultivars had the greatest yields from somewhat well section. Chungju had the greatest yields throughout all three sections compared to other cultivars. Chungju had 81% greater yield than Hongeon which had the smallest yield from somewhat well section. Arari set in middle from all sections. However there was no significant differences yields from very poor and somewhat poor sections. Leaf SPAD values tended to decrease and stable carbon isotope values increased as soil moisture increased. However, Chungju had no difference across different soil moistures in SPAD and stable carbon isotope values, while Hongeon had the greatest differences across sections. These trends followed by SDI values. Chungju had the smallest SDI values compared to other cultivars, which meant that Chungju was the strongest tolerance against excessive soil moisture than other cultivars. All three cultivars showed severe decrease of yields from very poor and somewhat poor sections. Arari and Hongeon showed great decrease from somewhat well section compared to yields from upland soil. These two cultivars may not be proper cultivating in paddy fields. In conclusion, adzuki bean is very sensitive to soil moisture condition and detailed soil managements are required to obtain optimal yields of adzuki bean from paddy field cultivation.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.584-589
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• 2007

The effect of water extract of adzuki beans on acetaminophen-altered lipid metabolism was examined in rats. Control group of rats was fed a basal diet, another group of rats was fed 0.5% acetaminophen (APAP group), and a third group of rats was fed 0.5% acetaminophen plus 5% adzuki bean extract (ABE group) for 4 weeks. Serum total and HDL cholesterol levels in the APAP group were significantly lower than those in the control and ABE groups. Hepatic cholesterol $7{\alpha}-hydroxylase$ and fatty acid synthase mRNA levels in the APAP and ABE groups were significantly higher and lower than in the control group, respectively. Hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase mRNA level in the APAP group was significantly lower than in the control group, whereas that in the ABE group was significantly higher than in the APAP group. These results indicate that adzuki bean extract may improve the acetaminophen-altered serum lipid metabolism in rats.

• The Korean Journal of Food And Nutrition
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• v.29 no.1
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• pp.134-143
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• 2016

We evaluated the antioxidant properties of adzuki beans and the quality characteristics of sediment using various cultivation methods. There were significant differences in total polyphenol and flavonoid contents in beans grown using different methods of cultivation (p<0.05). Also, DPPH and ABTS radical-scavenging activities were significantly different depending on cultivation method (p<0.05). The sediment yield before drying of Chungju-pat, Hongeon, and Arari was 296.64~339.01, 271.36~282.24, and 268.21~292.32%, respectively, and the sediment yield after drying was 71.68~85.41, 77.90~85.19, and 74.15~78.65%, respectively. The L-value of Chungju-pat and Arari sediments revealed a significant difference given different cultivation methods (p<0.05), but Hongeon sediment did not show a significant difference. There was a significant difference in the a- and b-value of adzuki bean sediments cultivated using different methods (p<0.05). The particle size of Chungju-pat, Hongeon, and Arari sediments was 66.21~98.80, 61.62~97.07, and $82.96{\sim}106.71{\mu}m$, respectively, and all were significantly different depending on cultivation method (p<0.05). There were also significant differences in the water absorption index, water solubility index, and swelling power when different cultivation methods were used (p<0.05).

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.218-218
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• 2017

Adzuki bean (Vigna angularis) has been extensively investigated due to their biological activities. In this study, total polyphenol content (TPC), total phenolic acid content (TPA), and total flavonoid content (TFC) in 209 Korean adzuki bean landraces were determined by colorimetric methods. Antioxidant effects were evaluated with the DPPH, ABTS, ferric reducing antioxidant power (FRAP), reducing power (RP), and SOD assays. TPC, TPA, and TFC in the 209 Korean adzuki bean landraces ranged from 1.1 to 11.7 mg gallic acid equivalents/g, 0.37 to 5.03 mg caffeic acid equivalents/g, and 0.17 to 0.91 mg quercetin equivalents/g, respectively. Antioxidant activities as assessed by the DPPH, ABTS, FRAP, PR, and SOD assays showed wide variation, ranging from 12.2 to 86.3 (IC50), 0.85 to 5.25 mg ascorbic acid equivalents (ASC)/g, 0.41 to 5.44 mg ASC/g, 0.54 to 1.83 mg ASC/g, and 60.4 to 142.8 (IC50), respectively. Using the relative antioxidant capacity index (RACI), we found that the IT189394 sample had the highest antioxidant activity. In clustering analysis, 209 Korean adzuki bean landraces were classified into three clusters. Among them, cluster I contained 22 accessions with higher antioxidant activities, TPC, TFC, and TPA and smaller seed sizes than the other clusters. We anticipate that these results will provide useful information for the development of adzuki bean-based functional foods.

• The Korean Journal of Mycology
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• v.50 no.4
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• pp.319-329
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• 2022

In July 2020, wilting symptoms were observed among adzuki bean plants (Vigna angularis var. angularis L.) in the fields in Yeosu, Korea. Infected plants showed yellowing of leaves, browning inside the stems, splitting of stem bark, and wilting. When these plants were uprooted, their roots were found to be brown. The fungal pathogens NC20-737, NC20-738, and NC20-739 were isolated from symptomatic stem and root tissues. These pathogens were identified as a Fusarium fujikuroi species complex based on their morphological characteristics. Molecular identification was performed using the DNA sequence of translation elongation factor 1 alpha and the RNA polymerase II second largest subunit regions. The nucleotide sequences of all three isolates were similar to the F. fujikuroi reference isolates NRRL 13566 and NRRL 5538 of the National Centre for Biotechnology Information GenBank. A pathogenicity test was conducted by the soil inoculation method with cornmeal sand inoculum. Approximately 3 weeks after inoculation, symptoms were observed only in the inoculated adzuki bean seedlings. To the best of our knowledge, this is the first report of Fusarium root rot caused by F. fujikuroi in adzuki beans, both in Korea and worldwide.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.67-75
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• 2022

Roots play important roles in water and nutrient uptake and in response to various environmental stresses. Investigating diversification of cultivars through root phenotyping is important for crop improvement in adzuki beans. Therefore, we analyzed the morphological and architectural root traits of 22 adzuki bean cultivars using 2-dimensional (2D) root imaging. Plants were grown in plastic tubes [6 cm (diameter) × 40 cm (height)] in a greenhouse from July 25^th to August 28^th. When the plants reached the 2^nd or 3^rd trifoliate leaf stage, the roots were removed and washed with tap water to remove soil particles. Clean root samples were scanned, and the scanned images were analyzed using the WinRHIZO Pro software. The cultivars were analyzed based on six root phenotypes [total root length (TRL), surface area (SA), average diameter (AD), and number of tips (NT) were included as root morphological traits (RMT); and link average length (LAL) and link average diameter (LAD) were included as root architectural traits (RAT)]. According to the analysis of variance (ANOVA), a significant difference was observed between the cultivars for all root morphological traits. Distribution analysis demonstrated that all root traits except LAL followed a normally distributed curve. In the correlation test, the most important morphological trait, TRL, showed a strong positive correlation with SA (r = 0.97^***) and NT (r = 0.94^***). In comparison, between RMT and RAT, TRL showed a significantly negative correlation with LAL (r = -0.50^***); however, TRL did not show a correlation with LAD. Based on RMT and RAT, we identified the cultivars that ranked 5% from the top and bottom. In particular, the cultivar "IT 236657" showed the highest TRL, SA, and NT, while the cultivar "IT 236169" showed the lowest values for TRL, SA, and NT. In addition, the coefficient of variance for the six tested root traits ranged from (14.26-40%) which suggested statistical variability in root phenotypes among the 22 adzuki bean varieties. Thus, this study will help to select target root traits for the adzuki bean breeding program in the future, generating climate-resilient adzuki beans, especially for drought stress, and may be useful for developing biotic and abiotic stress-tolerant cultivars based on better root trait attributes.

• Korean Journal of Agricultural Science
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• v.15 no.1
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• pp.69-75
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• 1988

This experiment was carried out for the analysis of the ability of callus formation and differentiation in the eight Korean local varieties of adzuki beans (Phaseolus angularis). KLA 102 has the highest ability on the formation of callus (all tissues have 90 % over). Morphological heterogeneity (color and shape) of callus was well reflected to the cell constitution and cell meristematic activities. Meristemoid tissues and rooting were derived from only creamish-white color and soft region of callus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.5
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• pp.687-694
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• 2015

The purpose of this study was to evaluate the yield, antioxidant content, and antioxidant activity of adzuki beans according to germination time. Cultivated varieties were Vigna angularis var. Nipponensis cv. Chungju-pat (CJP), and Yeonduchae (YDC), and Vigna radiata (L.) R. Wilczek cv. Dahyeon (DH). The moisture, crude protein, calcium, and magnesium contents of YDC significantly changed with increasing germination time, whereas potassium, natrium, and aluminium contents did not significantly change. Sprout yield, total polyphenol, flavonoid, and tannin contents of ethanolic extracts from adzuki and mung beans significantly increased with increasing germination time. Total polyphenol contents of ungerminated CJP, YDC, and DH were 1.96, 2.68, and 2.02 mg/g, and those of CJP and YDC germinated for 144 h were 3.33 and 3.47 mg/g, respectively. Total flavonoid content of adzuki beans substantially decreased with increasing germination time. Total tannin content substantially increased with increasing germination time, and YDC showed higher contents (0.85 mg/g) sample germinated for 120 h. DPPH radical scavenging activities of CJP and YDC substantially decreased with increasing germination time, whereas that of DH increased. ABTS radical scavenging activities of ungerminated CJP, YDC, and DH were 4.57, 6.51, and 2.82 mg/g, respectively, and increased after germination for 72~120 h.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.211-221
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• 2022

Recently, the demand for the cultivation of upland soil has been increasing, and the rate of conversion of paddy soil into upland soil is also increasing. Theincrease in uneven precipitation due to climate change has resulted in dramatic effects of waterlogging stress on upland crops. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the two-leaf stage of adzuki beans. The domestic cultivar, Arari (Miryang No. 8), was used to test waterlogging stress. At the two-leaf stage of adzuki beans, plant height slightly decreased androot fresh weight showed significant changes after 3 days of waterlogging treatment. Chlorophyll content was also significantly different after 3 days of waterlogging treatment compared to its content in control plants. Using two-dimensional gel electrophoresis, more than 400 protein spots were identified. Twenty-one differentially expressed proteins from the two-leaf stage were analyzed using linear trap quadrupole-Fourier transform-ion cyclotron resonance mass spectrometry. Of these 21 proteins, 9 were up-regulated and 12 were down-regulated under waterlogging treatment. Protein information resource (https://pir.georgetown.edu/) categories were assigned to all 49 proteins according to their molecular function, cellular component localization, and biological processes. Most of the proteins were found to be involved in the biological process, carbohydrate metabolism and were localized in chloroplasts.

• YAKHAK HOEJI
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• v.22 no.2
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• pp.72-82
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• 1978

This study was undertaken establish the relationship between trypsin inhibitor in raw soybean and antinutritional effect of raw legumes. 1) Among legumes produced in Korea, Glycine max contains a relatively high amount of protein(higher than 40%) compared with kindey bean, sword bean and mung bean and, furthermore, soybean which contains a high amount of protein possesses high trypsin inhibitory activity. 2) Disc electrophoretic pattern exhibited pattern exhibited that the crude protein preparation from Glycine max produced about 9-12 protein bands, and the pattern of electrophoretic mobility was very similar to each other. However, only a few protein bands were observed from the crude protein preparation of yard long bean, sword bean, adzuki bean, mung bean and rice adzuki. From the eluate of the sliced gel, it was confirmed that among those bands, only the fastest moving band contains trypsin inhibitory activity. 3) In chicks fed the normal diet the body weight was increased steady from one week and reached to 40% increase for three weeks but in chick fed raw bean diet, there was no body weight gain until two weeks feeding and only 10-20% of body weight gain was observed at the end of three week feeding. On the other hand, in chicks fed raw bean diet the weight of pancreatic tissue per 100g body weight was increased to about two-fold for two or three weeks but there was no change in liver weight. 4) In the case of amylase secretion from the pancreatic fragment, very strong stimulation on amylase secretion from pancreatic tissue of chicks fed a normal diet was produced by one unit of cholecystokin-pancreozymin. However, no stimulation was observed from pancreatic fragment of chick fed raw bean diet.