KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Growth Characteristics and Comparative Proteome Analysis of Adzuki Bean Leaves at the Early Vegetative Stage under Waterlogging Stress

논 토양 조건에서 팥 유묘기의 생육특성과 단백질 발현 양상

  • Hae-Ryong Jeong (Department of Crop Science, Chungbuk National University) ;
  • Soo-Jeong Kwon (Department of Crop Science, Chungbuk National University) ;
  • Sung-Hyun Yun (Department of Crop Science, Chungbuk National University) ;
  • Min-Young Park (Department of Crop Science, Chungbuk National University) ;
  • Hee-Ock Boo (WELLPHYTO Co. Ltd.) ;
  • Hag-Hyun Kim (Department of Food Nutrition and Cookery, Woosong College) ;
  • Moon-Soon Lee (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Sun-Hee Woo (Department of Crop Science, Chungbuk National University)
  • 정해룡 (충북대학교 식물자원학과 ) ;
  • 권수정 (충북대학교 식물자원학과 ) ;
  • 윤성현 (충북대학교 식물자원학과 ) ;
  • 박민영 (충북대학교 식물자원학과 ) ;
  • 부희옥 ((주)웰파이토 ) ;
  • 김학현 (우송정보대학교 식품영양조리학부 ) ;
  • 이문순 (충북대학교 특용식물학과 ) ;
  • 우선희 (충북대학교 식물자원학과 )
  • Received : 2022.10.24
  • Accepted : 2022.11.22
  • Published : 2022.12.01
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Abstract

Recently, the demand for the cultivation of upland soil has been increasing, and the rate of conversion of paddy soil into upland soil is also increasing. Theincrease in uneven precipitation due to climate change has resulted in dramatic effects of waterlogging stress on upland crops. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the two-leaf stage of adzuki beans. The domestic cultivar, Arari (Miryang No. 8), was used to test waterlogging stress. At the two-leaf stage of adzuki beans, plant height slightly decreased androot fresh weight showed significant changes after 3 days of waterlogging treatment. Chlorophyll content was also significantly different after 3 days of waterlogging treatment compared to its content in control plants. Using two-dimensional gel electrophoresis, more than 400 protein spots were identified. Twenty-one differentially expressed proteins from the two-leaf stage were analyzed using linear trap quadrupole-Fourier transform-ion cyclotron resonance mass spectrometry. Of these 21 proteins, 9 were up-regulated and 12 were down-regulated under waterlogging treatment. Protein information resource (https://pir.georgetown.edu/) categories were assigned to all 49 proteins according to their molecular function, cellular component localization, and biological processes. Most of the proteins were found to be involved in the biological process, carbohydrate metabolism and were localized in chloroplasts.

논 토양 조건에서 팥의 생육기 중 초기 생육 단계에 해당되는 유묘기에 과습 스트레스 처리 후 생육특성 변화 및 팥잎의 단백질 발현 양상을 조사한 결과는 다음과 같다. 1. 아라리(밀양 8호)를 공시 품종으로 과습 스트레스 실험을 진행하였다. 3일간 과습 처리하는 동안 팥의 초장과 생체중(잎, 줄기)은 통계적으로 유의하지는 않았으나 생체중(뿌리)와 SPAD value 값은 통계적으로 유의한 결과가 나왔다. 2. 과습 처리 3일 후 잎의 단백질 발현 양상을 확인한 결과 21개의 차별 발현된 단백질 중 과습 스트레스 처리에 의해 9개의 단백질들이 up-regulated 되었고, 12개의 단백질들이 down-regulated 되었다. 단백질을 기능별로 분류한 결과 Biological Process에서 carbohydrate metabolic process와 관련된 단백질들이 가장 많이 나왔고 Cellular Component에서는 Chloroplast에서 가장 많이 존재하였다. 3. Regulatory protein과 관련된 Maturase K 단백질은 처리구에서 대조구보다 발현양이 증가한 것으로 나타났다. 4. Carbohydrate metabolic process와 관련된 Malate dehydrogenase 단백질과 Glyceraldehyde-3-phosphate dehydrogenase 단백질은 과습 스트레스를 받았을 경우 단백질 발현양이 증가하였다. 5. Photosynthesis와 관련된 Carbonic anhydrase (CA)는 처리구보다 대조구에서 단백질 발현량이 더 증가한 것을 확인할 수 있었다. 스트레스와 관련된 단백질 Superoxide dismutase는 처리구에서 대조구보다 단백질 발현양이 증가한 것을 확인할 수 있었다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(세부과제명: 주요 밭작물의 논 재배 확대를 위한 안정생산 기술 개발, 세부과제번호: PJ01228605)의 지원에 이루어진 것이며, 이의 지원에 감사드립니다.

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