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http://dx.doi.org/10.7740/kjcs.2022.67.4.211

Growth Characteristics and Comparative Proteome Analysis of Adzuki Bean Leaves at the Early Vegetative Stage under Waterlogging Stress  

Hae-Ryong Jeong (Department of Crop Science, Chungbuk National University)
Soo-Jeong Kwon (Department of Crop Science, Chungbuk National University)
Sung-Hyun Yun (Department of Crop Science, Chungbuk National University)
Min-Young Park (Department of Crop Science, Chungbuk National University)
Hee-Ock Boo (WELLPHYTO Co. Ltd.)
Hag-Hyun Kim (Department of Food Nutrition and Cookery, Woosong College)
Moon-Soon Lee (Department of Industrial Plant Science & Technology, Chungbuk National University)
Sun-Hee Woo (Department of Crop Science, Chungbuk National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.67, no.4, 2022 , pp. 211-221 More about this Journal
Abstract
Recently, the demand for the cultivation of upland soil has been increasing, and the rate of conversion of paddy soil into upland soil is also increasing. Theincrease in uneven precipitation due to climate change has resulted in dramatic effects of waterlogging stress on upland crops. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the two-leaf stage of adzuki beans. The domestic cultivar, Arari (Miryang No. 8), was used to test waterlogging stress. At the two-leaf stage of adzuki beans, plant height slightly decreased androot fresh weight showed significant changes after 3 days of waterlogging treatment. Chlorophyll content was also significantly different after 3 days of waterlogging treatment compared to its content in control plants. Using two-dimensional gel electrophoresis, more than 400 protein spots were identified. Twenty-one differentially expressed proteins from the two-leaf stage were analyzed using linear trap quadrupole-Fourier transform-ion cyclotron resonance mass spectrometry. Of these 21 proteins, 9 were up-regulated and 12 were down-regulated under waterlogging treatment. Protein information resource (https://pir.georgetown.edu/) categories were assigned to all 49 proteins according to their molecular function, cellular component localization, and biological processes. Most of the proteins were found to be involved in the biological process, carbohydrate metabolism and were localized in chloroplasts.
Keywords
adzuki bean; growth characteristics; proteome; vegetative stage; waterlogging stress;
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