• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.213-213
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• 1996

The effects of the anti-tumor agent, SDZ-y2434, on rat kidney were investigated to predict the toxicities of its derivatives and to develope less toxic derivatives. After adjusted in metabolic cages for 5 days, rats were treated SDZ-62434(acute : 25mg/kg, i.p, once and 50mg/kg, i.p., once; subacute ; 10mg/kg, i.p., daily for 7 days). Kidney weights and urine volume during the treatment were observed. Creatinine concentration, protein concentration and the activities of N-acetyl-${\beta}$-D-glucosaminidase (NAG), alanine aminopeptidase (AAP), ${\gamma}$-glutamyl transpeptidase (GGT) and lactate dehydrogenase(LDH) in 24 hr urine were also determined. The kidney weights after the acute and subacute administration didn't show any difference. Urine volume increased 5 days after the acute administration (50mg/kg) and 3 days after the subacute administration. The excretion of creatinine was increased 5 days after the acute (50mg/kg) and subacute administration. However, the protein excretion didn't show any change. NAG acivity declined 7 days after the subacute administration. AAP and GGT activites increased 3 days after the acute administration (50mg/kg) but, returned to the control value. LDH activity showed continuousely high value after the subacute administration. These results indicates that the acute administration of SDZ-62434 might damage on glomerulus and that the subacute administration might be cytotoxic to kidney cells.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2082-2089
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• 2015

Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. Using the preferred codon of Pichia pastoris, the mature AvBD9 peptide was designed and synthesized, based on the sequence from GenBank. The P. pastoris constitutive expression vector pGHKα was used to construct a pGHKα-AvBD9 recombinant plasmid. Restriction enzyme digestion was performed using SacI and BglII to remove the ampicillin resistance gene, and the plasmid was electrotransformed into P. pastoris GS115. High-expression strains with G418 resistance were screened, and the culture supernatant was analyzed by Tricine-SDS-PAGE and western blot assay to identify target bands of about 6 kDa. A concentrate of the supernatant containing AvBD9 was used for determination of antimicrobial activity. The supernatant concentrate was effective against Escherichia coli, Salmonella paratyphi, Salmonella pullorum, Pseudomonas aeruginosa, Enterococcus faecalis, and Enterobacter cloacae. The fermentation product of P. pastoris carrying the recombinant AvBD9 plasmid was adjusted to 1.0 × 10⁸ CFU/ml and added to the drinking water of white feather broilers at different concentrations. The daily average weight gain and immune organ indices in broilers older than 7 days were significantly improved by the AvBD9 treatment.

• Journal of Nutrition and Health
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• 제50권6호
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• pp.578-584
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• 2017

Purpose: Excess intake of sodium is a major diet-related risk factor for human diseases including hypertension and cancer as well as obesity and inflammation. However, findings are still controversial, and evidence is lacking in Koreans. Therefore, for better understanding of the role of dietary sodium intake in disease etiology, this study investigated the effects of dietary sodium intake on adiposity, inflammation, and hormones in Koreans. Methods: A total of 80 males and females joined the study. The general characteristics and dietary intake data were investigated by trained interviewers using a questionnaire and 24-h dietary recall, respectively. For the markers of adiposity, body weight, body mass index, percent of body fat, visceral fat area, and waist and hip circumference were measured. For the inflammation and hormone markers, leptin, adiponectin, insulin, tumor necrosis $factor-{\alpha}$, and interleukin-6 were also analyzed. Results: Multivariate linear regression analyses suggested that dietary sodium intake was not associated with adiposity. However, dietary sodium showed a significant association with insulin level: Plasma insulin concentration increased with sodium intake independent of other dietary intake or percent of body fat (${\beta}=0.296$, adjusted $r^2=0.276$, p < 0.01). Other markers for inflammation and hormonal responses were not associated with dietary sodium intake. Conclusion: Findings suggested that dietary sodium intake may be a critical modifying factor in the level of plasma insulin. However, it showed a limited effect on obesity and other inflammation markers and hormone levels. These findings should be confirmed in larger, well-designed investigations.

• Journal of Radiation Protection and Research
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• 제45권2호
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• pp.88-94
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• 2020

Background: The International Commission on Radiological Protection (ICRP) has recently published report series on the occupational intakes of radionuclides (OIR) for internal dosimetry of radiation workers. In this study, the optimized monitoring program including the monitoring interval and the minimum detectable activity (MDA) of major radionuclides was suggested to perform the routine individual monitoring of internal exposure based on the ICRP OIR. Materials and Methods: The derived recording levels and the critical monitoring quantities were reviewed from international standards or guidelines by the International Atomic Energy Agency (IAEA), the International Organization for Standardization (ISO), and the European Radiation Dosimetry Group (EURADOS). The OIR data viewer provided by ICRP was used to evaluate the monitoring intervals and the MDA, which are derived from the reference bioassay functions and the dose coefficients. Results and Discussion: The optimal monitoring intervals were determined taking account of two requirement conditions on the potential intake underestimation and the MDA values. The MDA requirement values of the selected radionuclides were calculated based on the committed effective dose from 0.1 mSv to 5 mSv. The optimized routine individual monitoring program was suggested including the optimal monitoring intervals and the MDA requirements. The optimal MDA values were evaluated based on the committed effective dose of 0.1 mSv. However, the MDA can be adjusted considering the practical operation of the routine individual monitoring program in the nuclear facilities. Conclusion: The monitoring intervals and the MDA as crucial factors for the routine monitoring were described to suggest the optimized routine individual monitoring program of the occupational intakes. Further study on the alpha/beta-emitting radionuclides as well as short lived gamma-emitting nuclides will be necessary in the future.

• 재무관리연구
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• 제22권2호
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• pp.135-164
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• 2005

개인투자자 10,000명의 1998년부터 2003년까지 6년간의 거래자료와 잔고자료를 분석한 결과, 개인투자자들은 총수익률(gross return) 기준으로 연간 12.3%의 수익률을 실현한 것으로 나타났다. 동기간에 거래소 시장의 가치가중평균수익률은 13.6%였으며 코스닥 시장을 포함하는 종합시장수익률은 9.7%를 기록하였다. 그러나 거래비용을 고려한 순수익률(net return)은 연간 8.3%로 하락하여 시장수익률보다 크게 낮은 것으로 나타났는데, 연간 270%가 넘는 거래 회전율이 투자성과에 부정적인 영향을 미친 탓이라 할 수 있다. 특히 잔고규모별 초과수익률 분석에서는 투자금액이 상위 20%에 속하는 투자자들은 시장수익률과 비슷한 수준의 수익률을 얻은 반면, 나머지 80%의 투자자들은 시장수익률과 커다란 차이를 보였는데, 왜 많은 개인 투자자들이 시장을 떠나는지를 확인시켜 주는 결과이다. 특기할 사항은 과잉확신으로 인해 거래량이 증가하고 기대효용은 낮아진다는 과잉확신 모형을 지지한다는 점이다. 분석대상인 개인투자자들은 고 베타 주식과 소형주와 가치주를 선호하는 것으로 나타났다.

• 한국건설관리학회논문집
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• 제7권5호
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• pp.94-104
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• 2006

주택공급제도 개편의 일환으로 공공택지에서 공급되는 $85m^2$이하 주택에 대하여 분양가상한제의 확대시행과 분양가 주요항목에 대한 원가공개를 골자로 하는 주택법의 개정에 따라 건축비 산정체계의 현실적인 개편의 필요성이 대두되고 있다. 분양가상한제는 택지비와 건축비를 연동시켜 분양가를 결정하는 제도로, 택지비는 공공기관이 관계법에 의하여 개발 후 공급하는 택지가격에 의하여 결정되지만, 건축비는 정부에서 발표하는 상한가격내에서 결정하도록 하는 제도로서 분양가상한제의 주요쟁점은 적정 건축비 상한가를 책정하는 것이다. 이에 본 연구에서는 적정 건축비를 추정하는 방안을 실제 가상모델사업을 중심으로 제시하고 신축적 운영방안을 제안하였다. 다양한 자료수집 분석 및 전문가들의 의견 수렴 결과, 대한주택공사 예정가격을 기초로 산정한 1,028천원/$m^2$(공급면적, 지하주차장공사비 제외)이 적정한 수준으로 나타났으며, 지하층면적, 구조형식, 단지환경수준, 소비자선호도등에 따라 신축적으로 운영되어야 한다는 결과를 얻었다. 본 연구에서 밝힌 연구과정 및 결과는 정부의 부동산관련 정책수립은 물론 학계의 유사 연구에 일조할 것으로 기대된다.

• Genomics & Informatics
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• 제8권2호
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• pp.76-80
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• 2010

Although autism spectrum disorder (ASD) has been thought to have a substantial genetic background, major contributing genes have yet to be identified or successfully replicated. Immunological dysfunction has been suggested to be associated with ASD, and T cell-mediated immunity was considered important for the development of ASD. In this study, we analyzed 163 ASD subjects and 97 normal controls by genomic quantitative PCR to evaluate the association between the copy number variation of the 7q34 locus, harboring the TCRB gene, and ASDs. As a result, there was no significant difference of the frequency distribution of TCRB copy numbers between ASD cases and normal controls. TCRB gene copy numbers ranged from 0 to 5 copies, and the frequency distribution of each copy number was similar between the two groups. The proportion of the individuals with <2 copies of TCRB was 52.8% (86/163) in ASD cases and 57.1% (52/91) in the control group (p=0.44). The proportion of individuals with >2 copies of TCRB was 11.7% (19/163) in ASD cases and 12.1% (11/91) in the control group (p=0.68). After the effects of sex were adjusted by logistic regression, ORs for individuals with <2 copies or >2 copies showed no significant difference compared with the diploid copy number as reference (n=2). Although we could not see the positive association, our results will be valuable information for mining ASD-associated genes and for exploring the role of T cell immunity further in the pathogenesis of ASD.

• 한국학교ㆍ지역보건교육학회지
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• 제16권1호
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• pp.27-38
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• 2015

Objectives: The purpose of this study is to develop programs that help students adjust to school and provide basic data for the students who have difficulty adjusting to school by analyzing the influence of elementary school students' stress and their relationship with parents on school adjustment. Methods: The study surveyed fourth graders in 3 elementary schools in G district. The researcher used convenience sampling to select the 3 elementary schools. The combined 158 students were selected for the analysis, and the survey was conducted for a month in September 2011. The stress monitoring tool used in the 5th year of Korean Youth Panel Survey was mobilized to measure levels of stress among the participants. To measure the parent-child relationship, the Korean Parent-Child Relationships Scale was used. For the measurement of school adjustment, a tool designed to identify the school adjustment of elementary school students was used. The SPSS 21 software and a two-sided test under the significance level of ${\alpha}=0.05$ were used to analyze data. The regression analysis was also used to identify the relative influence of the stress and parent-child relationship on children's adjustment to school. Results: The regression analysis has found the following: the higher peer stress (B=-0.175) and parental strictness (B=-0.159) grow, the lower the level of school adjustment gets; the higher the level of intimacy between parent and child gets, the higher the level of school adjustment grows; and intimacy between parent and child (${\beta}=0.400$) has the largest influence on the child's school adjustment. Conclusions: For elementary school students to be well adjusted to school, their stress and relationships with their parents should be managed. In particular, using the programs that can reduce stress from peers and increase parent-child intimacy will be effective in helping students adjust to school.

• Journal of Preventive Medicine and Public Health
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• 제54권6호
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• pp.471-480
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• 2021

Objectives: Cadmium is widely used, leading to extensive environmental and occupational exposure. Unlike other organs, for which the harmful and carcinogenic effects of cadmium have been established, the hepatotoxicity of cadmium remains unclear. Some studies detected correlations between cadmium exposure and hepatotoxicity, but others concluded that they were not associated. Thus, we investigated the relationship between cadmium and liver damage in the general population. Methods: In total, 11 838 adult participants from National Health and Nutrition Examination Survey 1999-2015 were included. Urinary cadmium levels and the following liver function parameters were measured: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), total bilirubin (TB), and alkaline phosphatase (ALP). Linear and logistic regression analyses were performed to assess the associations between urinary cadmium concentrations and each liver function parameter after adjusting for age, sex, race/ethnicity, annual family income, smoking status, alcohol consumption status, physical activity, and body mass index. Results: The covariate-adjusted results of the linear regression analyses showed significant positive relationships between log-transformed urinary cadmium levels and each log-transformed liver function parameter, where beta±standard error of ALT, AST, GGT, TB, and ALP were 0.049±0.008 (p<0.001), 0.030±0.006 (p<0.001), 0.093±0.011 (p<0.001), 0.034±0.009 (p<0.001), and 0.040±0.005 (p<0.001), respectively. Logistic regression also revealed statistically significant results. The odds ratios (95% confidence intervals) of elevated ALT, AST, GGT, TB, and ALP per unit increase in log-transformed urinary cadmium concentration were 1.360 (1.210 to 1.528), 1.307 (1.149 to 1.486), 1.520 (1.357 to 1.704), 1.201 (1.003 to 1.438), and 1.568 (1.277 to 1.926), respectively. Conclusions: Chronic exposure to cadmium showed positive associations with liver damage.

• 한국환경보건학회지
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• 제49권6호
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• pp.353-361
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• 2023

Background: Noise has been linked to an increased risk of various health issues, including stress, anxiety, insomnia, and cardiovascular disorders. However, research on the relationship between residential traffic noise and mental health in South Korea is limited. Objectives: This study aims to examine the correlation between regional traffic noise levels and mental health using an ecological study design. Methods: In this ecological study, all data were collected from the Korean Statistical Information Service. Traffic noise data in residential areas and the prevalence of mental health indices in 44 cities with noise monitoring stations were obtained. Pearson correlation coefficients were calculated to assess the relationship between regional traffic noise levels and mental health indices. Results: Daytime traffic noise levels were found to have a positive correlation with stress perception (r=0.428, p=0.004) and showed a borderline positive correlation with depressive symptom prevalence (r=0.273, p=0.073). In cities with airports, significant correlations was observed between 24-hour average noise levels and depressive symptoms (r=0.604, p=0.010), whereas this relationship was not observed in cities without airports (r=0.048, p=0.813). Multiple regression analysis, which adjusted for factors such as age, gender ratio, smoking, obesity, unemployment, urban size, and the presence of an airport, confirmed the relationship between 24-hour average noise levels and stress perception (beta=0.287, p=0.024). Conclusions: This study identified correlations between regional traffic noise levels and depressive symptoms or stress perception in adults, with a more pronounced effect in areas with airports. These findings suggest a potential impact of residential traffic noise on mental health, highlighting the importance of preventive measures like noise management in urban planning to promote mental well-being.