Construction of Recombinant Pichia pastoris Carrying a Constitutive AvBD9 Gene and Analysis of Its Activity |
Tu, Jian
(College of Animal Science and Technology, Anhui Agricultural University)
Qi, Kezong (College of Animal Science and Technology, Anhui Agricultural University) Xue, Ting (College of Animal Science and Technology, Anhui Agricultural University) Wei, Haiting (College of Animal Science and Technology, Anhui Agricultural University) Zhang, Yongzheng (College of Animal Science and Technology, Anhui Agricultural University) Wu, Yanli (College of Animal Science and Technology, Anhui Agricultural University) Zhou, Xiuhong (College of Animal Science and Technology, Anhui Agricultural University) Lv, Xiaolong (College of Animal Science and Technology, Anhui Agricultural University) |
1 | Shan YJ, Hu Y, Xu WJ, Shu JT, Zhu CH, Li HF, et al. 2012. Codon optimization and its expression of chicken β-defensin-1 gene in eukaryocyte. J. Yunnan Agric. Univ. 27: 833-839. |
2 | Lynn DJ, Higgs R, Gaines S, Tierney J, James T, Lloyd AT, et al. 2004. Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken. Immunogenetics 56: 170-177. DOI |
3 | Li MH, Qu KZ, Tu J, Zhong J. 2012. Heterologous expression of chicken beta-defensin-7 in Escherichia coli and antimicrobial activity analysis. Microbiol. China 12: 1778-1786. |
4 | Lachica RVF, Genigeorgis C, Hoeprich PD. 1971. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Environ. Microbiol. 21: 585-587. |
5 | Kloster M, Tang C. 2008. SCUMBLE: a method for systematic and accurate detection of codon usage bias by maximum likelihood estimation. Nucleic Acids Res. 36: 3819-3827. DOI |
6 | Gustafsson C, Govindarajan S, Minshull J. 2004. Codon bias and heterologous protein expression. Trends Biotechnol. 22: 346-353. DOI |
7 | Xiao YJ, Hughes AL, Ando J, Matsuda Y, Cheng JF, Skinner-Noble D, Zhang G. 2004. A genome-wide screen identifies a single beta-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensins. BMC Genomics 5: 56. DOI |
8 | Wang SF, Sun YC, Ke F. 2012. Constitutive expression of Yarrowia lipolytica lipase LIP2 in Pichia pastoris using GAP as promoter. Appl. Biochem. Biotechnol. 166: 1355-1367. DOI |
9 | Wang HY. 208. The fusion expression and purification of chicken β-defensin-2(Gal-2) gene in Escherichia coli. Poult. Sci. 12: 7-10. |
10 | Wang HX, Miao L, Qi KZ. 2012. Expression of Gal-2 mature peptide gene in Pichia pastoris and analysis of antimicrobial activity of the recombinant protein. Chinese Vet. Sci. 42: 719-724. |
11 | Van Dijk A, Veldhuizen EJ, Kalkhove SI, Tjeerdsma-van Bokhoven JL, Romijn RA, Haagsman HP. 2007. The β-defensin gallinacin-6 is expressed in the chicken digestive tract and has antimicrobial activity against food-borne pathogens. Antimicrob. Agents Chemother. 51: 912-922. DOI |
12 | Sugiarto H, Yu PL. 2004. Avian antimicrobial peptides: the defense role of beta-defensins. Biochem. Biophy. Res. Commun. 323: 721-727. DOI |
13 | Tian XM, Ren JH, Fang C. 2012. Proteomic analysis of Pichia pastoris GS115 cultured in different carbon source mediums. China Biotechnol. 32: 21-29. |
14 | Sunkara LT, Achanta M, Schreiber NB, Bommineni YR, Dai G, Jiang W, et al. 2011. Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression. PLoS One 6: 1-10. DOI |
15 | Sun N, Sun D, Zhu D. 1996. Molecular Genetics. Nanjing University Press, Nanjing. |
16 | Zhao X, Huo KK, Li YY. 2000. Synonymous codon usage in Pichia pastoris. Chin. J. Biotechnol. 16: 308-311. |
17 | Zhang ZP, Yang JK, Xu L, Liu Y, Yan Y. 2010. Cloning codon optimization and expression of mature lipase gene Penicillum expansum. Acta Microbiol. Sinica 50: 228-235. |
18 | Zhang HH, Yang XM, Xie QM. 2009. Expression and secretion of chicken β-defensin-3 in Pichia pastoris. Guangdong Agric. Sci. 3: 123-126. |
19 | Yang JL, Wang MS, Cheng AC, Pan KC, Li CF, Deng SX. 2008. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection. World J. Gastroenterol. 14: 2872-2876. DOI |
20 | Xing HY, Gao YJ, Ning GB, Zhao JZ, Qiao YL, Ia ZY. 2007. Regulation of the expression of gallinacin-3 in Escherichia coli and re-folding of the recombinant protein. Chin. J. Vet. Sci. 27: 184-187. |