• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.75-81
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• 2016

Glutaraldehyde was used as a cross-linking agent for immobilization of purified ${\alpha}$-amylase from Exiguobacterium sp. DAU5. Befitting concentration of glutaradehyde and cross-linking time is the key to preparation of cross-linking chitosan beads. Based on optimized immobilization condition for ${\alpha}$-amylase, an overall yield of 56% with specific activity of 2,240 U/g on chitosan beads and 58% with specific activity of 2,320 U/g on chitosan-carbon beads was obtained. The optimal temperature and pH of each immobilized enzyme activity were $50^{\circ}C$ and 50 mM glycine-NaOH buffer pH 8.5, respectively. Those retained more than 75 and 90% of its maximal enzyme activity at pH 7.0-9.5 and after incubation at $50^{\circ}C$ for 1 h, respectively. In addition, the immobilization product showed higher organic-solvent tolerance than free enzymes. The mode of hydrolyzing soluble starch revealed that the ${\alpha}$-amylase possessed high hydrolyzing activity. These results indicate that chitosan is good support and has broad application prospects of enzyme immobilization.

• Safety and Health at Work
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• v.11 no.3
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• pp.367-371
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• 2020

We evaluated the eating pattern, physical activity, and daytime sleepiness level in Chilean shift workers. Fifty, middle-aged adult health workers from a public hospital in Santiago, Chile, were included: a group undergoing shift work (shift workers, including at least one "night shift" and one "long day", n = 33), and day workers under traditional schedule (from 8:00 to 17:00h, n = 17). Body composition, physical activity, and daytime sleepiness levels, and diet characteristics (diet composition, meals' timing, and diet quality) were assessed. Despite similar total energy intake, shift worker showed lower carbohydrate (% of energy) and higher protein intake (both P < 0.01), decreased diet quality, an irregular eating pattern, and delayed meal timing (all P < 0.05). Physical activity and daytime sleepiness levels did not differ between groups. Findings from this first Chilean study in healthcare shift workers support the fact that meal timing and diet quality appear as critical factors for upcoming intervention studies in this group.

• The Journal of Korean Physical Therapy
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• v.26 no.2
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• pp.130-135
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• 2014

Purpose: The purpose of this study was to examine the impairments of body structures, activity limitation, and participation restriction. In addition, we wanted to provide basic data on correlation between impairments of body structures, activity, and participation in Disabled Persons Living at Home. Methods: After selection of 128 people with physical disabilities more than the third level and brain damage disabilities living at home, we conducted testing for impairments of body structures, activity, and participation, according to the ICF checklist. Results: Impairments of body structures was highest in the upper and lower extremity. 2) Mobility, domestic life, and self care were more limited. 3) Also, the structure related to movement showed correlation with mobility, domestic life, and self care. Conclusion: We observed differences in participation and activities of persons with disabilities Living at Home depending on the impairments of body structures. After thorough review of the status of Disabled Persons Living at Home, we decided to appropriate support and social services.

• Journal of International Academy of Physical Therapy Research
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• v.1 no.2
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• pp.185-191
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• 2010

Background: This study investigated effective posture for gluteus medius rehabilitation training and effects of isometric muscle activity by electrophysiology through EMG while performing dynamic isotonic behavior of weight placed differently on upper limbs. Method: 16 healthy male subjects 20 to 29 years of age volunteered for the study. Lateral stabilizer right gluteus medius activity was assessed using EMG while the right lower extremity maintains single limb support, and the left upper extremity elevation movement maintains 5 seconds without load, 1RM to 1 repetition, 5RM to 5 times, 10RM to 10 times, 5RM and 10RM maintain 5sec. Results: Comparison of the mean value of EMG data showed a statistically more significant difference in upper extremity elevation movement on opposite upper extremity added weight than one that was not added on a single limb weight bearing posture(p>.05). Weight supported side gluteus medius activity for 1RM, 5RM, 10RM weight difference and movement repetition did not differ(p>.05). Comparison in maximum value showed statistically significant differences in not adding weight on upper limb elevation exercise and 1RM, 5RM, 10RM repeated behavior. Elevation behavior and repetition appeared over 70% of MVIC. Conclusion: Unilateral weight bearing stance added weight in the opposite upper limb elevation movement was an indirect exercise to effectively stimulate gluteus medius activity. Applying various added weight will have effective exercise on the early stages of rehabilitation because activity gluteus medius did not differ through added weight.

• International Commerce and Information Review
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• v.7 no.1
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• pp.121-144
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• 2005

This paper describes a theoretical model for investigating the relationship commitment of trade portal site 6C's. This research is the first to empirical test the causal relationships in Trade portal site. The relationship commitment for e-Trade portal site are tested here using data from 195 samples. Research methodology used in this study was mainly survey and questionnaire. Structural equation model is used in the hypotheses test. the results support all hypotheses involving hypothesis 1 and 6. The major finding of this paper would be summarized as followed: 1) Perceived relationship commitment of e-Trade site 6C's is positively related to contents, community, communication, customization in e-trade activity level and activity range of trade practical 2) Perceived commerce of electronic trade site is negatively related to the relationship commitment of trade companies. 3) Perceived e-trade site in electronic trade connection is positively related to the re-activity intention. In examining the relationships commitment of the factors, e-trade potal 6C's show in significantly by Contents, Community, Communication, Customization, Re-activity intention, but that show in not significantly by Commerce factor.

• The Journal of the Korea Contents Association
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• v.9 no.3
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• pp.296-304
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• 2009

The purpose of this study were to examine the effect of two different pelvic alignments and the back belt on electromyographic(EMG) activity of the erector spinae during lifting. The results were as follows: 1) EMG activity of erector spinae was greater when the pelvis was tilted anteriorly than when the pelvis was tilted posteriorly during lifting. 2) EMG activity of erector spinae was greater when the back belt than when without the back belt during lifting. These results suggest that the greater EMG activity of erector spinae with an anterior pelvic tilt position and with back belt during lifting may ensure optimal muscular support for the spine while handling loads.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.9-19
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• 2006

The purpose of this study was to analyze the kinematic variables of the lower limbs joints and the muscle activity for lower limbs during the level and downhill running. The subjects were 6 males of twenties and required to run on the level and downhill which was -7% grade treadmill at 8.3km/h. The running performances were filmed by high speed video camera and EMG signal was gained by ME3000P8 Measurement Unit. Rectus femoris(RF), Vastus lateralis(VL), Gluteus medius(GLU), Biceps femoris(BF), gastrocnemius medial head(GM), gastrocnemius lateral head(GL), Soleus(SO), Tibialis anterior(TA) were selected. The result of this study were as follows: 1. Ankle, knee, hip joint in downhill running showed less movement than the level running but, no significant difference. 2. VL and BF during the support phase in downhill running showed Iess muscle activity than the level running. but RF showed the opposite result. 3. GM, GL, SO adn TA during the supports phase in downhill running showed less muscle activity than the level running.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.10
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• pp.1093-1106
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• 2020

The role of the social environment for any activity is crucial as it prompts new ideas and plays a role of lever for entrepreneurial longevity and creating value through social wellbeing. The lack of entrepreneurship related knowledge in Pakistan has taken the challenge of lesser entrepreneurial activity as compared to other counterpart Asian countries and ranked 138 out of 189 countries on the level of ease of doing business. The study aims to investigate the social environment as a mediator among idea generation, business creation, financing decision and entrepreneurial activity by utilizing quantitative approach. In order to achieve the objective, a total of thirty eight items scale was self-administered for data collection. A non-probability, snowball sampling technique was employed and assembled a total of 300 complete responses through online and paper based surveys as per information provided by the chamber of commerce of three regions namely; Sukkur, Hyderabad and Karachi Sindh, Pakistan. The data analysis result strongly supported the main research question as the entrepreneurial activity increased up to 43.8% after applying social environment as a mediator. This research can help to enhance entrepreneurial activities in Pakistan by creating awareness to support entrepreneurs at social level for entrepreneurship.

• Korean Journal of Childcare and Education
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• v.13 no.2
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• pp.149-164
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• 2017

Objective: This study aimed to examine the mediation effects of parenting style and daily activity on the relationships between cumulative parental stress and cognitive and language development of children. Methods: The participants of this study were 1,154 mothers, their five-year-old children, and teachers enrolled in the population-based study of the Panel Study of Korea Children (PSKC) conducted by the Korea Institute of Childcare and Education from 2008 to 2013. In order to analyze the data, descriptive analysis, Pearson correlation analysis, and structural equation modeling were used. Results: The results revealed that warm parenting style as well as daily activity had dual mediating effects between cumulative parental stress and child development (cognitive and language development). However, warm parenting style did not have any direct effects on child development in this model. Conclusion/Implications: Given that actual mother-child daily activity had an important role in affecting child development, support programs for parenting should include activities that include specific materials and activities which parents can do with their children in everyday life.

• Animal cells and systems
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• v.14 no.2
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• pp.91-98
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• 2010

Male rats were given a single injection of iron, and temporal changes in iron content and iron-induced effects were examined in heart cellular fractions. Over a period of 72 h, the contents of total and labile iron, reactive oxygen species, and NO in tissue homogenate, nuclear debris, and postmitochondrial fractions were mostly constant, but in mitochondria they continuously increased. An abrupt decrease in membrane potential and NAD(P)H at 12 h was also found in mitochondria. The respiratory control ratio was reduced slowly with a slight recovery at 72 h, suggesting uncoupling by iron.While the ATP content of tissue homogenate decreased steadily until 72 h, it showed a prominent increase in mitochondria at 12 h. Total iron and calcium concentration also progressively increased in mitochondria over 72 h. Enzyme activity of the oxidative phosphorylation system was significantly altered by iron injection: activities of complexes I, III, and IV were reduced considerably, but complex II activity and the ATPase activity of complex V were enhanced. A reversal of activity in complexes I and II at 12 h suggested reverse electron transfer due to iron overload. These results support the argument that mitochondrial activities including oxidative phosphorylation are modulated by excessive iron.