• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.143-152
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• 1999

Dansam, the root of Salvia miltiorrhiza Bge, (Labiatae), has a bitter taste and a slightly 'cold' property, and is nontoxic. In the present study, effect of Dansam on nitric oxide (NO) generation from peritoneal macrophags was examined. Dansam had no effect on NO generation by itself, whereas recombinant interferon-${\gamma}\;(rIFN-{\gamma})$ alone had modest activity. When Dansam was used in combination with $rIFN-{\gamma}$, there was a marked cooperative induction of NO generation in a dose-dependent manner, The optimal effect of Dansam on NO generation was shown at 6 hr after treatment with $rIFN-{\gamma}$. Furthermore, the effect of Dansam was mainly dependent on Dansam-induced tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ secretion. These results suggest that Dansam induces NO generation from macrophages by the result of Dansam-induced $TNF-{\alpha}$ secretion.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.4
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• pp.401-407
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• 1991

Anticarcinogenic conjugated dienoic derivatives of linoleic acid (CLA) is present in grilled beef, cheese, and related foods, CLA is generated via isomerization of linoleic acid in the cow's rumen by anaerobic bacteria and food proceessing as well. Another source of CLA is its endogenous generation via the carbon centered free radical oxdation of linoleic acid. We propose that the formation and generation of CLA in vivo represents a previously unrecognized in situ "defense mechanism" against membrane attack by oxygen free radicals. The cis, 9-trans, 11 CLS isomer is selectively incorporated into cellular phospholipid, which exhibits a potent antioxidant, reduces the activation of 2-amino-3-methylimidazo, [4,5-f] quinoline (IQ) for baxterial mutagenesis, and inhibits ornithine decarboxylase(ODC) activity induced by 12-0-tetradecanoylphorbol-13-acetate (TPA). We believe that at least these biological activities of CLA explain the anticarcinogenic activity of CLA.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.9 no.2
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• pp.23-28
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• 1995

We experimented white mice for investigation on the effects of FIR Using special equipment for generation and survival activity. Because of no difference of consumption of water and food, rate of impregnation and birth, sexual ratio of second generation and weight between natural condition, FIR is proved no harmful effect on living organisms. In addition, We observed the survival activity of white mice at the severe circumstances. The survival activity of white mice under FIR circumstance increased 122% than that of mice under natural condition As the result, we surveyed the fact that FIR has good effect on white mice for physical strength and endurance ability.

• The Journal of Korean Medicine
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• v.29 no.1
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• pp.95-105
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• 2008

Objectives : Although herbal medicines containing flavonoids have been reported to exert anti-tumor activities, it has not been explored whether Hwang-Jeong (Polygonati sibirici Rhizoma, PsR) exerts anti-tumor activity in human glioma. The present study was therefore undertaken to examine the effect of PsR on cell viability and to determine its underlying mechanism in A172 human glioma cells. Methods : Cell viability was estimated by MTT assay. Reactive oxygen species generation and mitochondrial membrane potential were measured by the fluorescence dyes. The phosphorylation of kinases was evaluated by western blot analysis and caspase activity was estimated using colorimetric assay kit. Results : PsR resulted in loss of cell viability in a dose- and time-dependent manner. PsR did not increase reactive oxygen species (ROS) generation and the PsR-induced cell death was also not affected by antioxidants, suggesting that ROS generation is not involved in loss of cell viability. Western blot analysis showed that PsR treatment caused rapid reduction in phosphorylation of extracellular signal-regulated kinase (ERK) without changes in p38 and Jun-NH2-terminal kinase (JNK). U0126, an inhibitor of ERK, increased the PsR-induced cell death, but inhibitors of p38 and JNK did not affect the cell death. PsR induced depolarization of mitochondrial membrane potential. Caspase activity was not stimulated by PsR and caspase inhibitors did not prevent the PsR-induced cell death. Conclusion : Taken together, these findings suggest that PsR results in human glioma cell death through caspaseindependent mechanisms involving down-regulation of ERK.

• Journal of Korean Society of Transportation
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• v.30 no.3
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• pp.107-118
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• 2012

The purpose of the study is to analyze the travel characteristics of freight trucks in metropolitan areas, focusing on activity generation, destination choice, and trip chaining behaviors. The results showed that the number of service companies at departure areas has a primary influence on the activity generation pattern and destination choice behavior of trucks in metropolitan areas. The number of trips within a trip chain is largest, in case where the prevailing industry in destination areas is wholesale or retail and the shipment item is food or beverage. These results imply that for the reasonable estimation of truck travel demand both the trip chaining behaviors and the industrial compositions in departure and destination areas should be separately considered for each type of commodity.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.265-271
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• 2013

Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG $0.2{\mu}M$, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1322-1329
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• 2008

Mulberry has been reported to contain wide range of polyphenols and have chemopreventive activity. However, little has been known regarding the effect of mulberry fruits on cell viability in human glioma cells. The present study was undertaken to examine the effect of mulberry fruit (Mar; Fructus) on cell viability and to determine its underlying mechanism in human glioma cells. Cell viability and cell death were estimated by MTT assay and trypanblue exclusion assay, respectively. Reactive oxygen species (ROS) generation was measured using the fluorescence probe DCFH-DA. The mitochondrial transmembrane potential was measured with $DiOC_6$(3). Bax expression and cytochrome c release were measured by Western blot analysis. Caspase activity was estimated using colorimetric kit. Mori Fructus resulted in apoptotic cell death in a dose- and time-dependent manner. Mori Fructus increased ROS generation and the Mori Fructus-induced cell death was also prevented by antioxidants, suggesting that ROS generation plays a critical role in Mari Fructus-induced cell death. Western blot analysis showed that Mori Fructus treatment caused an increase in Bax expression, which was inhibited by the antioxidant N-acetylcysteine (NAC). Mori Fructus induced depolarization of mitochondrial membrane potential and its effect was inhibited by the antioxidants NAC and catalase. Mori Fructus induced cytochrome c release, which was inhibited by NAC. Caspase activity was stimulated by Mori Fructus and caspase inhibitors prevented the Mori Fructus-induced cell death. These findings suggest that Mori Fructus results in human glioma cell death through ROS-dependent mitochondrial pathway in human glioma cells.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.349-356
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• 2024

By 2025, South Korea is expected to enter an ultra-aged society with the elderly comprising 20.6% of the population. We measured changes in health status before and after by the use of a "Cognition-Emotion Enhancement Chatbot Integrated Product" among Baby Boomer generation women. Fifty participants, proficient in smart device usage and willing to provide data, were selected from health communities in Seoul. After excluding some applicants, 43 Baby Boomer women were analyzed. Results revealed significant differences in post-chatbot use physical activity (43.5.21 ± 1310.39 MET) and depression levels (6.84 ± 3.53). Correlation between the two variables was not statistically significant. The findings suggest specific effects of the chatbot on physical activity and depression, emphasizing the need for future research with diverse health indicators.

• Fashion & Textile Research Journal
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• v.10 no.5
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• pp.702-712
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• 2008

The aim of this study is to develop Casual Hanbok design made of Hanji yarn textiles for New Silver generation women. The New silver generation is a coined word which has meaning of a newly silver generation and it is distinguished from pre-silver generation. New silver generation is a generation that is independent and given active role by their age groups and generational characteristic. The concept of silver generation was introduced from maturity market in Japan. This study was based on analysis about their preference of forms, colors, and materials of Casual Hanbok. The survey target were 270 female over age 50 living in Daejeon City. The analysis methods used frequency and percentage. The results were summarized as following. Although almost of them haven't worn Casual Hanbok, they had the positive recognition on wearing one. They preferred longer length jacket to traditional Korean style, shorter length of skirt. They also preferred the traditional sleeve shape and knotted buttons. On the foundation of this fact, 2 styles consist of 5 Casual Hanbok design items made of functional material - Hanji yarn textiles that have antimicrobial, deodorant, quick drying, far infrared radiation which are not harmful to health - for New silver women were suggested. One style was 3 items - blouse, vest, skirt - which are the sense of Korean tradition with activity by patch. The other was 2 items - jacket and skirt - which are the sense of Korean tradition with modern way by quilting. Lastly, these garments were evaluated by 13 experts, they were satisfied with 2 styles and all items. As this study were based on the Elderly women living in Daejeon city, it had the limitation on applying of preference styles to all the New silver generation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.6 no.2
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• pp.45-55
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• 1986

This study proposes the explanatory indices of urban traffic for the purpose of solving the ambiguity of selection of the explanatory variables, which always raises problems in case of the travel-demand forecasting in the urban transportation planning, and develops optimal urban traffic generation models. The multiple regression models for objective traffic generation are developed by using the proposed explanatory inidces. Objective variables that can be explained by one explanatory variable are modified into simple regression type (Y=bX) in order to ensure the nonnegativity of traffic generation. Similarities are noted in the generaton characteristics of generated traffic from homogeneous land-use activity. Objective variables that can not be explained by multiple variable, such as trip attraction of school and trip generation of social-recreation, are classified by the characteristics of each zone. And traffic generation forecasting models are built as homogeneous zone group, the validity of each model being tested by a statistical method. It is desired that the forecasting precision is in improved by easy and simple method. Accordingly, trip generation rates are calculated from each land-use activity, and trip generation rates for practical application are proposed by considering their stability.