• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.427-433
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• 2008

The cephabacins produced by Lysobacter lactamgenus are ${\beta}$-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. In order to understand the precise implication of the polyketide synthase (PKS) module in the biosynthesis of cephabacin, the genes for its core domains, ${\beta}$-ketoacyl synthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), were amplified and cloned into the pET-32b(+) expression vector. The sfp gene encoding a protein that can modify apo-ACP to its active holo-form was also amplified. The recombinant KS, AT, apo-ACP, and Sfp overproduced in the form of $His_6$-tagged fusion proteins in E. coli BL21(DE3) were purified by nickel-affinity chromatography. Formation of stable peptidyl-S-KS was observed by in vitro acylation of the KS domain with the substrate [L-Ala-L-Ala-L-Ala-L-$^3H$-Arg] tetrapeptide-S-N-acetylcysteamine, which is the evidence for the selective recognition of tetrapeptide produced by nonribosomal peptide synthetase (NRPS) in the NRPS/PKS hybrid. In order to confirm whether malonyl CoA is the extender unit for acetylation of the peptidyl moiety, the AT domain, ACP domain, and Sfp protein were treated with $^{14}C$-malonyl-CoA. The results clearly show that the AT domain is able to recognize the extender unit and decarboxylatively acetylated for the elongation of the tetrapeptide. However, the transfer of the activated acetyl group to the ACP domain was not observed, probably attributed to the improper capability of Sfp to activate apo-ACP to the holo-ACP form.

• 한국환경과학회지
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• 제21권7호
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• pp.797-804
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• 2012

A dielectric barrier discharge (DBD) plasma reactor was investigated for the inactivation of Ralstonia Solanacearum which causes bacterial wilt in aquiculture. The DBD plasma reactor of this study was divided into power supply unit, gas supply unit and plasma reactor. The plasma reactor consisted of a quartz dielectric tube, discharge electrode (inner) and ground electrode (outer). The experimental results showed that the optimum 1st voltage, 2nd voltage, air flow rate and pH were for 100 V (1st voltage), 15 kV (2nd voltage), 4 L/min, and pH 3, respectively. At a low 1st voltage, shoulder and tailing off phenomena was observed. The shoulder phenomenon was decreased as the increase of 1st voltage. R. Solanacearum disinfection in the lower air flow rate was showed shoulder and tailing off phenomenon because the active species generated less. Under optimum condition, shoulder and tailing off phenomenon was reduced. When the 2nd voltage was less than 7.5 kV, tailing off phenomenon was observed and this was not vanishes even though the increase of the disinfection time. The inactivation efficiency increased as the increase of air flow rate, however, the efficiency decreased when the air flow rate was above 4 L/min. R. Solanacearum disinfection at pH 3 showed somewhat higher than in pH 11. The pH effect of R. Solanacearum deactivation is less than the impact on other factor.

• 한국환경과학회지
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• 제21권1호
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• pp.113-123
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• 2012

A unit emission reduction of nitrous oxide ($N_2O$) from anthropogenic sources is equivalent to a 310-unit $CO_2$ emission reduction because the $N_2O$ has the global warming potential (GWP) of 310. This greatly promoted very active development and commercialization of catalysts to control $N_2O$ emissions from large-scale stationary sources, representatively nitric acid production plants, and numerous catalytic systems have been proposed for the $N_2O$ reduction to date and here designated to Options A to C with respect to in-duct-application scenarios. Whether or not these Options are suitable for $N_2O$ emissions control in nitric acid industries is primarily determined by positions of them being operated in nitric acid plants, which is mainly due to the difference in gas temperatures, compositions and pressures. The Option A being installed in the $NH_3$ oxidation reactor requires catalysts that have very strong thermal stability and high selectivity, while the Option B technologies are operated between the $NO_2$ absorption column and the gas expander and catalysts with medium thermal stability, good water tolerance and strong hydrothermal stability are applicable for this option. Catalysts for the Option C, that is positioned after the gas expander thereby having the lowest gas temperatures and pressure, should possess high de$N_2O$ performance and excellent water tolerance under such conditions. Consequently, each de$N_2O$ technology has different opportunities in nitric acid production plants and the best solution needs to be chosen considering the process requirements.

• BMB Reports
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• 제32권4호
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• pp.414-418
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• 1999

A novel gene for malonyl-CoA decarboxylase was discovered in the mat operon, which encodes a set of genes involved in the malonate metabolism of Rhizobium trifolii (An and Kim, 1998). The subunit mass determined by SDS-PAGE was 53 kDa, which correspond to the deduced mass from the sequence data. The molecular mass of the native enzyme determined by field flow fractionation was 208 kDa, indicating that R. trifolii malonyl-CoA decarboxylase is homotetrameric. R. trifolii malonyl-CoA decarboxylase converted malonyl-CoA to acetyl-CoA with a specific activity of 100 unit/mg protein. Methylmalonyl-CoA was decarboxylated with a specific activity of 0.1 unit/mg protein. p-Chloromercuribenzoate inhibited this enzyme activity, suggesting that thiol group(s) is(are) essential for this enzyme catalysis. Database analysis showed that malonyl-CoA decarboxylase from R. trifolii shared 32.7% and 28.1% identity in amino acid sequence with those from goose and human, respectively, and it would be located in the cytoplasm. However, there is no sequence homology between this enzyme and that from Saccharopolyspora erythreus, suggesting that malonyl-CoA decarboxylases from human, goose, and R. trifolii are in the same class, whereas that from S. erythreus is in a different class or even a different enzyme, methylmalonyl-CoA decarboxylase. According to the homology analysis, Cys-214 among three cysteine residues in the enzyme was found in the homologous region, suggesting that the cysteine was located at or near the active site and plays a critical role in catalysis.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.552-560
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• 2020

Human carbonic anhydrase (CA) isozyme II has been used as protein target for disorder treatment including glaucoma. Current clinically used sulfonamide-based CA inhibitors can induce side effects, and so alternatives are required. This study aimed to investigate a natural CA inhibitor from Murraya paniculata. The previously developed yeast-based assay was used to screen 14 compounds isolated from M. paniculata and identified by NMR analysis for anti-human CA isozyme II (hCAII) activity. Cytotoxicity of the compounds was also tested using the same yeast-based assay but in a different cultivation condition. Two flavonoid candidate compounds, 5, 6, 7, 8, 3', 4', 5'-heptamethoxyflavone (4) and 3, 5, 7, 8, 3', 4', 5'-heptamethoxyflavone (9), showed potent inhibitory activity against hCAII with a minimal effective concentration of 10.8 and 21.5 μM, respectively, while they both exhibited no cytotoxic effect, even at the highest concentration tested (170 μM). The results from an in vitro esterase assay of the two candidates confirmed their hCAII inhibitory activity with IC₅₀ values of 24.0 and 34.3 μM, respectively. To investigate the potential inhibition mechanism of compound 4, in silico molecular docking was performed using the FlexX and SwissDock software. This revealed that compound 4 coordinated with the Zn²⁺ ion in the hCAII active site through its methoxy oxygen at a distance of 1.60 Å (FlexX) or 2.29 Å (SwissDock). The interaction energy of compound 4 with hCAII was -13.36 kcal/mol. Thus, compound 4 is a potent novel flavonoid-based hCAII inhibitor and may be useful for further anti-CAII design and development.

• Applied Biological Chemistry
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• 제40권4호
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• pp.352-356
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• 1997

식물배양세포계에서 유용 phytoalexin의 생산여부를 검토하기 위하여 페튜니아 배양세포주에 5종의 naphthoquinone류 화합물을 첨가하였을 때 유도되는 항미생물활성을 조사하였다. 처리한 naphthoquinone류 화합물 중 토양 방선균으로부터 페튜니아 세포생장억제물질로 분리한 2,5,7-trihydroxy-3-(5'-hydroxyhexyl)-1,4-naphthoquinone (3-OH NQ)이 48시간 이내에 90% 이상 효율적으로 배양세포내로 흡수되었다. 3-OH NQ를 처리한 페튜니아 캘러스 추출물은 Aspergillus candidus의 포자발아를 강하게 억제하였다$(MIC:\;32\;{\mu}g/ml)$. 3-OH NQ를 처리한 페튜니아 배양세포로부터 2종의 항미생물 활성물질 4,2',4',${\beta}$-tetrahydroxychalcone과 4',7-dihydroxyflavone을 분리하였다. 주로 유도되는 phytoalexin인 4,2',4',${\beta}$-tetrahydroxychalcone은 A. candidus의 포자발아를 강하게 억제하였다$(MIC:\;16\;{\mu}g/ml)$.

• 한국컴퓨터그래픽스학회논문지
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• 제26권4호
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• pp.17-26
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• 2020

본 논문에서는 GPU(Graphic Processing Unit) 연산을 활용하여 BCC(Body Centered Cubic) 볼륨 데이터로부터 실시간으로 메시 형태의 등가면을 추출하는 개선된 마칭 사면체(Marching tetrahedra) 기법을 제안한다. 본 기법은 고전적인 방법과 비교하여 메모리 사용량은 다소 높지만 더 좋은 성능을 보인다. 본 기법은 다섯 단계로 구성되어 있다. 첫 번째 단계는 단 한번만 수행되는 단계로, 빈 공간을 생략하여 성능을 향상 시키기 위해 최소/최댓값 블록(Min/max block)을 생성한다. 두 번째 단계에서는 등갓값(Isovalue)을 포함하고 있는 유효한 블록을 추려낸다. 이후 두 단계에서는 등가면(Isosurface)을 포함하는 셀(Cell)과 엣지(Edge)를 추출하고, 마지막 단계에서 삼각형 메시(Triangle mesh)를 생성한다. 본 기법은 512³ 이상의 고해상도 볼륨 데이터(Volume dataset)에 대한 등가면 추출 시, 삼각형 집합 형태의 등가면을 추출하는 고전적인 마칭 사면체 기법에 비해 최대 5배 정도의 속도 향상을 보인다.

• 한국주거학회논문집
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• 제17권1호
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• pp.145-153
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• 2006

Making of agreement with participation subjects is important process laying stress on presentation and a reasonable design guide line to form space order grant and synthetic space in Housing Complex plan. This study analyzed residing application specific character of design guide line that is presented to architects in general planning laying stress on plan only of 'Bongmu-dong town house' and design agreement of by architectural result that appear analyze. Result that analyze is as following: 1) Confer in 18 plan contents and integrate design or was adjusted. Being main conduct and unit plan and residing only in plan many negotiations accomplish. Architect who confer most Designs of 5 architects is 'Jean Michel Wilmott' and 'Shigeru Ban' 2) contents that confer much in plan main conduct and unit generation were details plan. Contents that is conferred with many architects are about door/core/rooftop be and asked a question about proper size of entrance. Negotiation about size was expose to the tribe of knowledge about element that do furniture and detail of necessary each space in life style of our country. We must present furnitures or detail element that appear by emotion of our country and detailed item of furnitures' size etc.. in guide line. It is immediate that creation of guide that architects can approach easily for lacking abroad architects of interests by code difference in each country is pressing. 3) In residing plan 4 architects of 5 architects applied similarly guide line in design. Most architects look by active support that make synthetic housing complex. and this is construed that act positively to make by unified residing. That plan of woods or landscape architecture and security of green area space are thing to approach on 'Environment-friendly mode of life residing only' that is general planning subject of 'Bongmu-dong town house'. 4) common question items of architects guide line of though is refered definitely when make out effective interests plan. So that can overcome legislation difference, countermeasure to make understood construction code of our country is pressing

• 대한전자공학회논문지SD
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• 제40권9호
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• pp.685-691
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• 2003

본 논문에서는 고속 통신 시스템 응용을 위한 12b 100 MS/s CMOS D/A 변환기(DAC) 회로를 제안한다. 제안하는 DAC는 전력소모, 면적, 선형성 및 글리치 에너지 등을 고려하여, 상위 8b는 단위 전류셀 매트릭스 (unit current-cell matrix)로 나머지 하위 4b는 이진 전류열 (binary-weighted array)로 구성하였다. 제안하는 DAC는 동적 성능을 향상시키기 위해 새로운 구조의 스위치 구동 회로를 사용하였다. 시제품 DAC회로 레이아웃을 위해서는 캐스코드 전류원을 단위 전류셀 스위치 매트릭스와 분리하였으며, 제안하는 칩은 0.35 um single-poly quad-metal CMOS 공정을 사용하여 제작되었다. 측정된 시제품의 DNL 및 INL은 12b 해상도에서 각각 ±0.75 LSB와 ±1.73 LSB이내의 수준이며, 100 MS/s 동작 주파수와 10 MHz 입력 주파수에서 64 dB의 SFDR을 보여준다. 전력 소모는 3 V의 전원 전압에서 91 mW이며, 칩 전체 크기는 2.2 mm × 2.0 mm 이다.

• 중환자간호학회지
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• 제13권1호
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• pp.27-43
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• 2020

Purpose : This integrative review aimed to synthesize studies on intensive care unit (ICU) nurses' attitude, perceptions, and experiences toward end-of-life care decision-making. Methods : Using Whittermore and Knafl (2005)'s methods, we identified and synthesized research articles published in domestic journals between the years 2003 and 2019 and evaluated the quality of selected articles using the Mixed Methods Appraisal Tool. Results : In the 13 studies reviewed, 12 were published prior to enactment of the "The Act for Hospice and Palliative Care and Decision-Making about Life-Sustaining Treatment (2018)." All nine quantitative studies identified were based on cross-sectional descriptive survey. In four qualitative studies, content analysis (n=2) and phenomenology (n=2) were used. Overall, ICU nurses were well-aware of the necessity of communicating and limiting life-sustaining treatments. Many ICU nurses had positive attitude towards limiting life-sustaining treatments to promote patients' comfort and dignity. Although nurses were willing to take active roles, they also reported having experienced high stress in the process of decision-making and implementation. Conclusions : It is important to prepare ICU nurses with proper knowledge and attitude regarding the topic area. It is also equally important to develop systems to support nurses' emotional stress and moral distress during communication, decision-making, and implementation.