• The Korean Journal of Physiology and Pharmacology
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• v.20 no.1
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• pp.9-14
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• 2016

Adenosine 3',5'-cyclic monophosphate (cAMP) participates in the regulation of numerous cellular functions, including the $Na^+-K^+$-ATPase (sodium pump). Ouabain, used in the treatment of several heart diseases, is known to increase cAMP levels but its effects on the atrium are not understood. The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Our results showed that ouabain ($3.0{\mu}mol/L$) significantly increased atrial dynamics and cAMP levels during recovery period. The ouabain-increased atrial dynamics was blocked by KB-R7943 ($3.0{\mu}mol/L$), an inhibitor for reverse mode of $Na^+-Ca^{2+}$ exchangers (NCX), but did not by L-type $Ca^{2+}$ channel blocker nifedipine ($1.0{\mu}mol/L$) or protein kinase A (PKA) selective inhibitor H-89 ($3.0{\mu}mol/L$). Ouabain also enhanced atrial intracellular cAMP production in response to forskolin and theophyline ($100.0{\mu}mol/L$), an inhibitor of phosphodiesterase, potentiated the ouabain-induced increase in cAMP. Ouabain and 8-Bromo-cAMP ($0.5{\mu}mol/L$) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 ($30{\mu}mol/L$), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Our results demonstrated that ouabain increases atrial cAMP levels and promotes atrial ET-1 secretion via the mitogen-activated protein kinase (MAPK)/ERK signaling pathway. These findings may explain the development of cardiac hypertrophy in response to digitalis-like compounds.

• Molecules and Cells
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• v.44 no.2
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• pp.88-100
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• 2021

Anoctamin 6/TMEM16F (ANO6) is a dual-function protein with Ca2+-activated ion channel and Ca2+-activated phospholipid scramblase activities, requiring a high intracellular Ca2+ concentration (e.g., half-maximal effective Ca2+ concentration [EC50] of [Ca2+]i > 10 μM), and strong and sustained depolarization above 0 mV. Structural comparison with Anoctamin 1/TMEM16A (ANO1), a canonical Ca2+-activated chloride channel exhibiting higher Ca2+ sensitivity (EC50 of 1 μM) than ANO6, suggested that a homologous Ca2+-transferring site in the N-terminal domain (Nt) might be responsible for the differential Ca2+ sensitivity and kinetics of activation between ANO6 and ANO1. To elucidate the role of the putative Ca2+-transferring reservoir in the Nt (Nt-CaRes), we constructed an ANO6-1-6 chimera in which Nt-CaRes was replaced with the corresponding domain of ANO1. ANO6-1-6 showed higher sensitivity to Ca2+ than ANO6. However, neither the speed of activation nor the voltage-dependence differed between ANO6 and ANO6-1-6. Molecular dynamics simulation revealed a reduced Ca2+ interaction with Nt-CaRes in ANO6 than ANO6-1-6. Moreover, mutations on potentially Ca2+-interacting acidic amino acids in ANO6 Nt-CaRes resulted in reduced Ca2+ sensitivity, implying direct interactions of Ca2+ with these residues. Based on these results, we cautiously suggest that the net charge of Nt-CaRes is responsible for the difference in Ca2+ sensitivity between ANO1 and ANO6.

• Fire Science and Engineering
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• v.24 no.1
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• pp.15-23
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• 2010

Placement method for fire detectors prescribed in current fire safety regulation is just about placing a prescribed number of detectors according to the areas. However, this regulation has no scientific basis and standards from foreign countries are just introduced and fire detectors are installed complying with them. There are two standards in designing fire protection systems; Prescriptive-Based Design that follows stipulated regulations like fire safety standards and Performance-Based Design based on engineering knowledge such as fire dynamics, structural dynamics, mechanics of materials, fluid mechanics, and thermo dynamics. Recently, Fire Protection System Construction Business Act was revised so that fire protection systems can be designed using Performance-Based Design method ('05. 8. 4), though the method has not activated until now. In addition, the enforcement decree defines the range for specific objects of fire protection to which Performance-Based Design is applied ('07, 1. 24). At the moment, by manufacturing simulator so that formulas can be introduced and calculated with software in order to install fire detector of automatic fire detection systems keeping optimized distance, comparing the results with the state of fire detector placed according to Performance-Based Design and analyzing them, this study was intended to settle Performance-Based Design method in the future.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.3
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• pp.293-300
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• 2017

Prostaglandin $D_2$ ($PGD_2$) may act against myocardial ischemia-reperfusion (I/R) injury and play an anti-inflammatory role in the heart. Although the effect of $PGD_2$ in regulation of ANP secretion of the atrium was reported, the mechanisms involved are not clearly identified. The aim of the present study was to investigate whether $PGD_2$ can regulate ANP secretion in the isolated perfused beating rat atrium, and its underlying mechanisms. $PGD_2$ (0.1 to $10{\mu}M$) significantly increased atrial ANP secretion concomitantly with positive inotropy in a dose-dependent manner. Effects of $PGD_2$ on atrial ANP secretion and mechanical dynamics were abolished by AH-6809 ($1.0{\mu}M$) and AL-8810 ($1.0{\mu}M$), $PGD_2$ and prostaglandin $F2{\alpha}$ ($PGF2{\alpha}$) receptor antagonists, respectively. Moreover, $PGD_2$ clearly upregulated atrial peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and the $PGD_2$ metabolite 15-deoxy-${\Delta}12$, 14-$PGJ_2$ (15d-$PGJ_2$, $0.1{\mu}M$) dramatically increased atrial ANP secretion. Increased ANP secretions induced by $PGD_2$ and 15d-$PGJ_2$ were completely blocked by the $PPAR{\gamma}$ antagonist GW9662 ($0.1{\mu}M$). PD98059 ($10.0{\mu}M$) and LY294002 ($1.0{\mu}M$), antagonists of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling, respectively, significantly attenuated the increase of atrial ANP secretion by $PGD_2$. These results indicated that $PGD_2$ stimulated atrial ANP secretion and promoted positive inotropy by activating $PPAR{\gamma}$ in beating rat atria. MAPK/ERK and PI3K/Akt signaling pathways were each partially involved in regulating $PGD_2$-induced atrial ANP secretion.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.10
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• pp.1090-1097
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• 2006

The anoxic activated sludge process was applied to the treatment of industrial box-mill wastewater, which exhibited the high removal efficiencies of $90{\sim}94%$$ TCOD_{Mn}$ and $58{\sim}81%$ Color. For the design of industrial anoxic activated sludge process, Monod bio-kinetic coefficients of box-mill wastewater were estimated as follows: $K_{max}$(maximum specific substrate removal rate)=0.52 $day^{-1}$, $K_s$(half saturation constant)=314 mg/L, $K_d$(decay coefficient)=0.274 $day^{-1}$, y(microbial yield coefficient)=0.908 mg/mg, and ${\mu}_{max}$(maximum specific growth rate)=0.472 $day^{-1}$. Space loading factors for the design analysis were practically determined as the values of F/M ratio=$0.043{\sim}0.07$ kg-$TCOD_{Mn}$/kg-SS-day, BOD space loading=$0.18{\sim}0.3$ kg-$TCOD_{Mn}/m^3-day$, and ${\theta}_x=6.8{\sim}26.4$ day when considering the relationship of these loading factors with growth dynamics of microorganisms, the F/M ratio that is inversely proportional to ${\theta}_x$ should be equivalent to ${\mu}_{max}$ in units, but exhibited the significant difference between theses two values. Therefore, it is considered that high safety factors are requested in the design of anoxic activated sludge process that is based on Monod bio-kinetics of microorganism.

• Molecules and Cells
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• v.42 no.12
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• pp.893-905
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• 2019

Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.86.1-86.1
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• 2013

Observations of a filament showing an activated barb recorded from the at the Dutch Open Telescope (DOT) on 2010 August 20 are presented. The filament developed a barb in 10 minutes, which disappeared within the next 35 minutes. Such a rapid formation and disappearance of a filament barb is unusual, and has been seldom reported. Line-of-sight velocity maps were constructed from images in seven line positions along the H-alpha line. We observe flows in the filament spine towards the barb location prior to its formation, and flows in the barb towards the spine during its disappearance. Photospheric magnetograms from Helioseismic Magnetic Imager on board the Solar Dynamics Observatory were used to determine the changes in magnetic flux in the region surrounding the barb location. The variation of magnetic flux in this duration support the view that barbs are rooted in minor magnetic polarity.

• Mass Spectrometry Letters
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• v.2 no.3
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• pp.73-75
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• 2011

In the collisionally-activated dissociation of the proton-bound cluster ions of DNA base guanine (G) and cytosine (C), $G{\bullet}{\bullet}H^+{\bullet}{\bullet}C$, the abundance of [$CH^+$] ions was found to be higher than that of [$GH^+$] despite the fact that G has a higher proton affinity than C. This unexpected observation seems to demonstrate another example that the simple kinetic method scheme does not work. We suggest that a kinetic factor or detailed dynamics governing the proton transfer and dissociation should be carefully considered in the applications of the kinetic method to the proton affinity measurements.

• Coupled systems mechanics
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• v.5 no.1
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• pp.21-39
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• 2016

Wastewater process models are the essential tools for understanding relevant aspects of wastewater treatment system. Wastewater process modeling provides more options for upgrades and better understanding of new plant design, as well as improvements of operational controls. The software packages (BioWin, GPS-X, Aqua designer, etc) solve a series of simulated equations simultaneously in order to propose several solutions for a specific facility. Research and implementation of wastewater process modeling in combination with computational fluid dynamics enable testing for improvements of flow characteristics for WWTP and at the same time exam biological, physical, and chemical characteristics of the flow. Application of WWTP models requires broad knowledge of the process and expertise in modeling. Therefore, an efficient and good modeling practice requires both experience and set of proper guidelines as a background.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.309-309
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• 2000

The main objective of this study is to understand the regeneration step of the PTSA(Pressure and thermal swing adsorption) process below the atmospheric pressure by rigorous dynamic simulation. This target process is to recover toluene using activated carbon as an adsorbent. To do this, the dynamic simulations for the regeneration step are performed at 360, 490, 590mmHg and at high temperature after the simulation of the adsorption step at latm and 298K. A mathematical model was developed to simulate the column dynamics of the adsorption systems. This model is based on non-equilibrium, non-isothermal and non-adiabatic conditions, and axial dispersion and heat conduction are also considered. Heat transfer resistances are considered in gas-solid, gas-column wall and column wall-outside air. The LDF(Linear Driving Force) approximation model describes the mass transfer rate between the gas and solid phase. This study shows that the recovery of toluene by PTSA is more preferable than that by general TSA.