• Molecules and Cells
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• 제42권2호
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• pp.123-134
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• 2019

Lysophosphatidic acid (LPA) is an endogenous lysophospholipid with signaling properties outside of the cell and it signals through specific G protein-coupled receptors, known as $LPA_{1-6}$. For one of its receptors, $LPA_1$ (gene name Lpar1), details on the cis-acting elements for transcriptional control have not been defined. Using 5'RACE analysis, we report the identification of an alternative transcription start site of mouse Lpar1 and characterize approximately 3,500 bp of non-coding flanking sequence 5' of mouse Lpar1 gene for promoter activity. Transient transfection of cells derived from mouse neocortical neuroblasts with constructs from the 5' regions of mouse Lpar1 gene revealed the region between -248 to +225 serving as the basal promoter for Lpar1. This region also lacks a TATA box. For the region between -761 to -248, a negative regulatory element affected the basal expression of Lpar1. This region has three E-box sequences and mutagenesis of these E-boxes, followed by transient expression, demonstrated that two of the E-boxes act as negative modulators of Lpar1. One of these E-box sequences bound the HeLa E-box binding protein (HEB), and modulation of HEB levels in the transfected cells regulated the transcription of the reporter gene. Based on our data, we propose that HEB may be required for a proper regulation of Lpar1 expression in the embryonic neocortical neuroblast cells and to affect its function in both normal brain development and disease settings.

• BMB Reports
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• 제52권2호
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• pp.133-138
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• 2019

Upon viral infection, the 2', 5'-oligoadenylate synthetase (OAS)-ribonuclease L (RNaseL) system works to cleave viral RNA, thereby blocking viral replication. However, it is unclear whether OAS proteins have a role in regulating gene expression. Here, we show that OAS1 and OAS3 act as negative regulators of the expression of chemokines and interferon-responsive genes in human macrophages. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) technology was used to engineer human myeloid cell lines in which the OAS1 or OAS3 gene was deleted. Neither OAS1 nor OAS3 was exclusively responsible for the degradation of rRNA in macrophages stimulated with poly(I:C), a synthetic surrogate for viral double-stranded (ds)RNA. An mRNA sequencing analysis revealed that genes related to type I interferon signaling and chemokine activity were increased in $OAS1^{-/-}$ and $OAS3^{-/-}$ macrophages treated with intracellular poly(I:C). Indeed, retinoic-acid-inducible gene (RIG)-I- and interferon-induced helicase C domain-containing protein (IFIH1 or MDA5)-mediated induction of chemokines and interferon-stimulated genes was regulated by OAS3, but Toll-like receptor 3 (TLR3)- and TLR4-mediated induction of those genes was modulated by OAS1 in macrophages. However, stimulation of these cells with type I interferons had no effect on OAS1- or OAS3-mediated chemokine secretion. These data suggest that OAS1 and OAS3 negatively regulate the expression of chemokines and interferon-responsive genes in human macrophages.

• Journal of Arbitration Studies
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• 제29권1호
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• pp.3-29
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• 2019

A franchise business is a business in which the owners, or "franchisors," sell the rights to their business logo, name, and model to third party retail outlets, owned by independent, third party operators, called "franchisees." There are a number of features in franchising or terms in franchise agreements that may lead to disputes between franchisors and franchisees. These disputes may arise because of underlying risks in the franchise relationship, franchise agreement, or conduct of the parties. In this case, ADR is an effective way to resolve disputes in a quicker and often less costly way than having to go to court. If an agreement cannot be reached through mediation, then arbitration becomes the next step to resolving the differences. Whereas mediation is non-binding and focused on facilitating the parties to find a resolution that is acceptable to both, arbitration is binding and may result in a decision that is not acceptable to one of the parties. These situations can be resolved through experienced arbitration as arbitration allows franchisees to settle matters promptly and outside of the public eye. In addition, franchise dispute arbitration is usually less costly than going to traditional court. Considering all of these, reaching an agreement will also have typical clauses that address the issue of dispute resolution. It is again a more efficient process than going through the legal process and courts and is often less costly. By going through arbitration, the parties agree to give up their rights to pursue the dispute in the courts. However, there is a problem that the arbitration prior to the agreement and under the terms would be contrary to the restriction of jurisdiction under the "ACT ON THE REGULATION OF TERMS AND CONDITIONS" in Korea.

• Journal of the Korean Society of Safety
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• 제35권6호
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• pp.85-92
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• 2020

Wet Scrubber reacts the incoming pollutant gas with cleaning water (water + absorbent) to absorb pollutants and release the clean air to the atmosphere. Wet scrubbers and packed tower scrubbers using this principle are widely used in businesses that emit acid gases. In particular, in the etching process using hydrochloric acid (HCl), alkaline washing water (NaOH) having a pH of about 8 to 11 is used to absorb a large amount of acid gas. However, These salts are attached to the injection nozzle (nozzle), filling material (packing), and the demister (Demister), causing air pollution, human damage, and inoperability due to clogging and acid gas discharge. Therefore, In this study, an improvement plan was proposed to manage the washing water with pH 3~4 acidic washing water. The test method takes samples from the Wet Scrubber flue measurement laboratory twice a month for 1 year. Hydrogen chloride (HCl) concentration (ppm) was measured, and nozzle clogging and scale conditions were measured, compared, and analyzed through a differential pressure gauge and a pressure gauge. As a result of the check, it was visually confirmed that the scale was reduced to 50% or less in the spray nozzle, filler, and demister. In addition, the emission limit of hydrogen chloride in accordance with the Enforcement Regulation of the Air Quality Conservation Act [Annex 8] met 3 ppm or less. Therefore, even if the washing water is operated in an acidic pH range of 3 to 4, it is expected to reduce air pollution and human damage due to clogging of internal parts, and it is expected to reduce maintenance costs such as regular cleaning or replacement of parts.

• Journal of Ginseng Research
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• 제45권3호
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• pp.456-463
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• 2021

Background: Keratinocytes form a physical barrier and act as an innate immune cell in skin. Keratinocytes secrete pro-inflammatory cytokines, such as interleukin (IL)-1β, resulting from inflammasome activation when exposed to ultraviolet (UV) irradiation. Korean Red Ginseng extracts (RGE) have been well-studied as modulators of inflammasome activation in immune cells, such as macrophages. In the study, we elucidated the role of RGE on the UV-mediated inflammasome activation in keratinocytes compared with that in macrophages. Methods: Human skin keratinocyte cells (HaCaT), human epidermal keratinocytes (HEK), human monocyte-like cells (THP-1), and mouse macrophages were treated with RGE or a saponin fraction (SF) or non-saponin fraction (NS) of RGE before and after UV irradiation. The secretion levels of IL-1β, as an indicator of inflammasome activation, were analyzed. Results: The treatment of RGE or SF in macrophages after UV irradiation inhibited IL-1β secretion, but similar treatment in HaCaT cells did not. However, the treatment of RGE or SF in HaCaT cells in the presence of poly I:C, a toll-like receptor (TLR) 3 ligand, before UV exposure elicited the inhibition of the IL-1β secretion. The inhibition was caused by the disruption by RGE or SF of the TLR mediating up-regulation of the pro-IL-1β and NLRP3 genes during the priming step. Conclusion: RGE and its saponins inhibit IL-1β secretion in response to UV exposure in both keratinocytes and macrophages. In particular, RGE treatment interrupted only the priming step in keratinocytes, although it did attenuate both the priming and activation steps in macrophages.

• Journal of Environmental Health Sciences
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• 제47권1호
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• pp.78-86
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• 2021

Objectives: The purposes of this study were to investigate the installation rates and status of indoor smoking rooms in public facilities and to determine the level of compliance level with smoke-free policies in accordance with the National Health Promotion Act. Methods: A visiting survey was conducted on 1,206 public facilities in the Seoul Metropolitan Area and the Daegu and Gyeongsangbuk-do Province area. Researchers selected public facilities such as bars, coffee houses, and internet cafes using convenient sampling. They visited without prior notice, checked the existence of indoor smoking rooms, and recorded their status. Results: Internet cafes (110/116) had the highest installation rate of indoor smoking rooms, followed by bowling clubs (17/19) and billiard rooms (87/100). Depending on the type of business, 50-88% of smoking rooms were not completely enclosed. Coin karaoke rooms showed the least inadequacy in this regard. In addition, out of 512 smoking rooms, in 33% (n = 169) smoking indoors was observed in non-smoking areas. Only 9% of public facilities were in full compliance with the indoor smoke-free regulation. Conclusions: It was found that most of the public facilities with indoor smoking rooms did not comply with the smoke-free policy, and smoking was still observed inside some facilities. Therefore, there is a need for a policy that prohibits indoor smoking rooms completely.

• Journal of the Korean Society for Aviation and Aeronautics
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• 제30권1호
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• pp.20-27
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• 2022

It is anticipated that there will be radical disputes over land ownership in the event of the advent of the era of UAM. As such, policy alternative is presented by analyzing and researching relevant laws at home and abroad on the means of legislating 'Regulations on compensation for use of air space above land' in preparation of such occurrence. As the results of the study, it is deemed to limit the range of the land ownership in accordance with UAM operation as follows. First, it is proposed to newly enact regulation to limit the ownership of air space of land owner to the public space above the elevation of 200m as stipulated under the Article 78 of the Aviation Safety Act. Second, as the result of analysis made for the option of making compensation from the perspective of the property right of land ownership and option of not making compensation from the perspectives of public interest and concerns in the event of operation of UAM within the air space below the elevation of 200m, it is deemed that legislative decision is necessary through more extensive studies in the future.

• Journal of the Korean Society of Safety
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• 제37권6호
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• pp.9-17
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• 2022

Qualifying quantities (upper tier (UT) and lower tier (LT)) are designated for the regulation of toxic substances. In this study, we aimed to establish systematic criteria for the qualifying quantities by comparing the South of Korea chemical control act with the European Seveso III Directive (Seveso III). In Seveso III, qualifying quantities are defined as "hazard categories" applying GHS (Globally Harmonized System of Classification and Labelling of Chemicals), and LTR (lower-tier requirements) and UTR (upper-tier requirements) are determined. The Pro HC (proposed hazard categories) were relevant to the GHS classification of toxic substances and were compared with the currently regulated qualifying quantities. Furthermore, we estimated the Pro LTR (proposed lower-tier requirements) and Pro UTR (proposed upper-tier requirements) corresponding to each Pro HC. Consequently, it was supposed that LT and UT were selected based on GHS like those of Seveso III. Therefore, designation criteria for qualifying quantities should be established by setting the Pro HC such as in Seveso III, rather than designating the qualifying quantities of toxic substances by itself individually. In addition, qualifying quantities should not be delegated to GHS classifications (H302, H341, H411) that do not meet the criteria for the designation of toxic substances, and the corresponding substances should be excluded from classification as toxic substances. This study provides insights into the selection of hazard categories and criteria for qualifying quantities of toxic substances.

• Journal of the Korean Society for Library and Information Science
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• 제56권3호
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• pp.93-117
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• 2022

This study attempted to analyze the contents of the public library steering committee-related ordinances, derive problems and implications, and based on this, suggest the contents and supplementations necessary for the construction of the library steering committee ordinance. To this end, first, we looked at the theoretical background such as the significance and role of the public library steering committee, and the nature of the ordinance. Second, it was analyzed by dividing it into five areas: the status of enactment of ordinances related to library steering committee, whether provisions related to library steering committee are included, composition content, composition method, function, conference and regulation enactment, and allowance payment. Based on this analysis, it was mentioned that it is necessary to establish a department in charge of ordinances, a library steering committee, guarantee the participation of library directors and library experts, hold regular meetings, disclose minutes, and reflect the results of committee deliberation. In addition, when enacting a standard ordinance items containing the name, nature and role, composition, meeting, sub-organization, rule of the library steering committee.

• Fashion & Textile Research Journal
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• 제24권4호
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• pp.471-478
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• 2022

In roadside workplaces, more attention should be paid to the safety of workers. The roadside workers underestimate the effect of the brightness of their clothes and judge that drivers will recognize them easily, and the drivers misjudge that the roadside workers are far away and that the vehicle can be stopped in sufficient time. Therefore, customized safety education reflecting this and wearing work clothes with certified visibility functions are required. In Korea, it is not compulsory for roadside workers and vehicle guide attendants to wear work clothes with a visibility function. In this study, the distance ahead perceived by drivers was measured using manikins wearing certified and non-certified reflective safety vests. The perception distance of the non-certified reflective safety vest was 1.4 times longer than that of the certified reflective safety vest, thus confirming the importance of wearing a certified reflective safety vest. To prevent roadside workers from suffering traffic accidents, we propose the enactment of a law that makes it mandatory for them to wear high-visibility safety clothing. Specifically, Article 32 of the Enforcement Regulation of the Road Traffic Act should include high-visibility safety clothing in life protection equipment, and additionally, to prevent secondary accidents, we propose the enactment of a law requiring the installation and wearing of certified reflective safety vests in vehicles.