• Natural Product Sciences
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• v.9 no.4
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• pp.264-269
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• 2003

The rhizome of Acorus calamus Linn. is commonly known as "Vacha" in indigenous systems of medicine. It is distributed in marshy tracts of Kashmir, Sirmaur (Himachal Pradesh), Manipur and the Naga hills. It is regularly cultivated in Koratagere Taluk in Karnataka and other parts of India. This study deals with the detailed pharmacognostical evaluation of the dried rhizomes of Acorus calamus collected from DehraDun (Uttaranchal), Lucknow (Uttar Pradesh). The commercial sample procured from Delhi market was also evaluated to observe the difference between collected and market samples. Dried rhizome is vertically compressed, pale yellow to dark brown and occasionally orangish brown in colour. Transverse section showed two distinct region with scattered, concentric vascular bundles surrounded by fibrous bundle sheath. Some vascular bundles just beneath the endodermis devoid of bundle sheath. Though the botanical and physico-chemical characters of all the samples were quite similar but some variations were observed in High Performance Thin Layer Chromatography (HPTLC) fingerprint profile, the essential oil content and total percentage of asarone which was found to be highest in Lucknow and lowest in Delhi market sample. These variations may be explained due to some edaphic factors or storage conditions. An attempt was also made to test antioxidant activity (in vitro) and it was found to be 88% at 0.2 g/ml concentration.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.232-239
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• 2011

The genus Acorus is known as an indigenous medicinal plant. Genetic diversity of thirteen accessions of A. calamus and eight of A. gramineus, with an accession of Colocasia antiquorum and two of Iris pseudacorus as outgroups, were evaluated using RAPD markers for cluster analysis and principal coordinate analysis, and NIR spectroscopic profiles for principal component analysis.A total of 371 polymorphic bands were obtained by using the selected 12 random primers. The genetic distances were estimated from 0.03 to 0.31 within A. calamus and from 0.03 to 0.51 within A. gramineus. The dendrogram and three-dimensional plot separated the accessions into four distinct groups (A. calamus, A. gramineus, C. antiquorum, and I. pseudacorus). Moreover, for the diversity among genus Acorus, eleven A. calamus accessions, one A. gramineus accession, and two I. pseudacorus accessions were non-destructively analyzed from their leaves by NIR spectroscopy, which discriminated Acorus accessions like the RAPD analysis. Interestingly, thirteen accessions of A. calamus were clustered into two groups based on RAPD and NIR analyses, which indicates that there are two ecotypes of A. calamus in Korea. An accession (CZ) of A. calamus with yellow stripe on leaves was closely grouped with another (CX) at a genetic distance (GD) of 0.03, which shows that the stripe trait might be generated by chimeric mutation. The genetic distance between A. calamus and A. gramineus was revealed to be farthest from 0.80 to 0.88 GD. In genus Acorus the genetic diversity and genetic variation were identified by using RAPD marker technique and non-destructive NIRs.

• Korean Journal of Environment and Ecology
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• v.19 no.1
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• pp.1-8
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• 2005

The removal affect of nitrogen and phosphorus were analyzed using a Acorus calamus var. angustatus. The nutrient concentration, growth stage of plants and the storage time of polluted water were considered. The results of this study were as follows: after an hour the content of nitrogen and phosphorus were considerably reduced in the Acorus calamus var. angustatus, while after two-four hours the rate of reduction was extremely low. This situation was the same in the early growth stage, growing stage and highest growth stage of the plant. The removal rate of nitrogen and phosphorus with the Acorus calamus var. angustatus was higher, when these two minerals remained in high levels of water. The Acorus calamus var. angustatus was more effective to remove nitrogen than that of phosphorus. The plant removed the most nitrogen and phosphorus when in the highest growth stage, but this was not clear in the growth stages. The removal rate was higher, in the case of moving polluted water to other plants after two days, than in the case of four days of growth in the same plants.

• Archives of Pharmacal Research
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• v.21 no.6
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• pp.734-737
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• 1998

The hexane fractions from methanolic extracts of Anetheum graveolens L. (Umbelliferae) and Acorus gramineus Soland. (Araceae) revealed potent inhibitory activities against the resistance of multi-drug resistant Staphylococcus aureus SA2 when combined with ampicillin (Am) or chloramphenicol (Cm). As active principles, carvone and the liquid mixture containing carvone from Anetheum graveolens L. and a liquid mixture mainly consisting of benzoic acid phenyl-methyl ester (benzyl benzoate) from Acorus gramineus Soland. were identified. They showed resistance inhibition at the level of 20-50${\mu}g$/ml when combined with 100 or ${\mu}g$/ml of Am or Cm, respectively.

• Applied Biological Chemistry
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• v.42 no.3
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• pp.210-217
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• 1999

To detect the toxic relevant components, asarone, coumarin and thujone, in the 87 medicinal plants used in brewing a Korean traditional folk wine, their 20% ethanol extracts were prepared and purified by a SPE(solid phase extraction) method. The toxic components in the purified extracts were identified by GC-FID and GC/MS analysis. On analyses, asarone was detected in 6 species, Acorus gramineus Solander, Acorus asiaticus Nakai, Angelia gigus Nakai, Santalum album, etc.; coumarin in 22 species, Anethum graveolens, Foeniculum vulgare Gaertner, Lithosperum erythrorhizon Siebold et Zuccarinii, etc.; thujone in 24 species, Achyranthes japonica Nakai, Amomum xanthiodes Wallich, Artemisia asiatia Nakai, seed of Cannabis sativa L., Caragana sinica R., Chrysanthemum morifolium Ramat, Codonopsis lanceolata Bentham et flooker, Foeniculum vulgare Gaertner, etc.

• Journal of Pharmacopuncture
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• v.25 no.4
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• pp.326-343
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• 2022

Neurological disorders represent a substantial healthcare burden worldwide due to population aging. Acorus gramineus Solander (AG) and Acorus tatarinowii Schott (AT), whose major component is asarone, have been shown to be effective in neurological disorders. This review summarized current information from preclinical and clinical studies regarding the effects of extracts and active components of AG and AT (e.g., α-asarone and β-asarone) on neurological disorders and biomedical targets, as well as the mechanisms involved. Databases, including PubMed, Embase, and RISS, were searched using the following keywords: asarone, AG, AT, and neurological disorders, including Alzheimer's disease, Parkinson's disease, depression and anxiety, epilepsy, and stroke. Meta-analyses and reviews were excluded. A total of 873 studies were collected. A total of 89 studies were selected after eliminating studies that did not meet the inclusion criteria. Research on neurological disorders widely reported that extracts or active components of AG and AT showed therapeutic efficacy in treating neurological disorders. These components also possessed a wide array of neuroprotective effects, including reduction of pathogenic protein aggregates, antiapoptotic activity, modulation of autophagy, anti-inflammatory and antioxidant activities, regulation of neurotransmitters, activation of neurogenesis, and stimulation of neurotrophic factors. Most of the included studies were preclinical studies that used in vitro and in vivo models, and only a few clinical studies have been performed. Therefore, this review summarizes the current knowledge on AG and AT therapeutic effects as a basis for further clinical studies, and clinical trials are required before these findings can be applied to human neurological disorders.

• Journal of Sasang Constitutional Medicine
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• v.11 no.1
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• pp.241-252
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• 1999

1. Purpose and Method : We have many difficulty of using the existing medical Hurbs based on the theory of Em-Yang and the five elements, this is why we still do not explain the Sasang Constitutional medical Hurb Classification and do not have the Sasang Constitutional Pharmacology exactly, so we easily enter into a dispute and confusion. So through literary consideration about Acorus gramineus Soland. I try to objectify Sasang Constitutional Classification of Acorus gramineus Soland and the spirit of using Acorus gramineus Soland and common property of Sasang Constitutional Medical Hurb and try to find out a clue that search the effect of other Sasang Constitutional Medical Hurb. 2. Result : Qi(氣) and mi(味) of Acorus gramineus Soland have aroma and hot taste and have won Qi(溫氣), the using portion of Acorus gramineus Soland is root as medical Hurb. So Acorus gramineus Soland rise from Goonghacho(中下焦) to Sangcho(上焦) and divied impurity and purity and able to remove the turbidity Qi(氣)Ack(液) Acorus gramineus Soland have the effect of progressing the involution of Paeqi(肺氣) and divided impurity and purity of Qi(氣) and ack(液) and improve the fuction and structure of Tae-Em-ln. I think that the method of literay consideration on objectification of Sasang Constitution Pharmacology is of great value.

• Environmental Engineering Research
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• v.20 no.1
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• pp.51-57
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• 2015

As expected, the expression of denitrifying genes in a Typha wetland (relatively stagnant compared to other ponds), showing higher nitrogen removal efficiency in summer, was affected by temperature. The abundance and gene transcripts of nitrate reductase (narG), nitrite reductase (nirS), nitric oxide reductase (norB), and nitrous oxide reductase (nosZ) genes in seasonal sediment samples taken from the Acorus and Typha ponds of free surface flow constructed wetlands were investigated using quantitative polymerase chain reaction (Q-PCR) and quantitative reverse transcription PCR (Q-RT-PCR). Denitrifying gene copy numbers ($10^5-10^8$ genes $g^{-1}$ sediment) were found to be higher than transcript numbers-($10^3-10^7$ transcripts $g^{-1}$ sediment) of the Acorus and Typha ponds, in both seasons. Transcript numbers of the four functional genes were significantly higher for Typha sediments, in the warm than in the cold season, potentially indicating greater bacterial activity, during the relatively warm season than the cold season. In contrast, copy numbers and expression of denitrifying genes of Acorus did not provide a strong correlation between the different seasons.

• Journal of Life Science
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• v.11 no.6
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• pp.503-508
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• 2001

The essential oil of Acorus gramineus(Aracease), which has been used as an antioconvulsant in Korean folk medicine, was evaluated for its effects on antioxidative system in vitro and in vivo. This mixture of terpenes showed inhibitory effects on xanthine oxidase activity with 13.3% at 10 $\mu\textrm{g}$/ml and on aldehyde oxidase activity with 5.0% at 1$\mu\textrm{g}$/ml. Lipid perosidation was inhibited by 49.4% at 1.0 mg/ml of the essential oil in vitro and by 16.7% after 7 days inhablation of an oil as compared to PTZ-treated control group. DPPH radical scavenging activity of this essential oil was ralatively werak.

• Food Science and Biotechnology
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• v.14 no.5
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• pp.685-688
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• 2005

Antibacterial and antiplatelet activities of Acorus gramineus rhizome-derived asaronaldehyde and asaron were analyzed using platelet aggregometer and six human intestinal bacteria. Active constituent of A. gramineus rhizome was isolated and characterized as asaronaldehyde by spectral analyses. At 2 and 1 mg/disk, asaronaldehyde exhibited strong inhibition of Clostridium perfringens and C. difficile without adverse effects on growth of beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus acidophilus, and L. casei. Asaron also revealed moderate growth inhibition against C. perfringens and C. difficile at 2 mg/disk, no growth-inhibiting activity was observed on B. bifidum, L. acidophilus, L. casei, and E. coli. At 50% inhibitory concentration ($IC_{50}$) value, asaronaldehyde was effective in inhibiting platelet aggregation induced by collagen ($IC_{50}$, $27.6\;{\mu}M$) and arachidonic acid ($IC_{50}$, $53.7\;{\mu}M$). These results suggest asaronaldehyde may be useful as lead compound for inhibiting platelet aggregation induced by collagen and arachidonic acid.