• 생약학회지
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• 제39권4호
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• pp.316-323
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• 2008

In this study, we have investigated the HPLC analysis methods and quantitative analysis of standard compounds for quality standardization of a medicinal crude drug GCSB-5, a herbal formulation consisting of 6 medicinal plants (Acanthopanacis Cortex, Achyranthis Radix, Ledebouriellae Radix, Cibotii Rhizoma, Glycine Semen, Eucommiae Cortex) which are used in traditional medicine to treat various bone disorders. HPLC analysis methods of acanthoside D(Acanthopanacis Cortex), 20-hydroxyecdysone(Achyranthis Radix) which were known standard compounds among 6 medicinal plants were developed on crude material and product. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. Content of acanthoside D and 20-hydroxyecdysone on raw material of GCSB-5 were decided at 0.577-0.578 mg/g and 0.311-0.312 mg/g. And we confirmed that content of acanthoside D and 20-hydroxyecdysone on GCSB-5 preparation were 0.302-0.303 mg/capsule and 0.113-0.115 mg/capsule.

• 동의생리병리학회지
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• 제19권5호
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• pp.1213-1219
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• 2005

• 대한한의정보학회지
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• 제15권1호
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• pp.77-84
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• 2009

Objectives : The aim of the present study is to evaluate the effects Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae extracton 3T3-L1 preadipocytes differentiation. Methods : We adopted Oil Red O staining methods to observe the formed lipid droplets. And the amount of lipids in adipocytes was measured using absorptiometric analysis. Results : The extracts of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae stimulated the preadipocytes differentiation and lipid droplet formation. And the complex extract of the traditional herbal medicines stimulated there actions more than single extracts. Conclusions : The extract of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae has stimulatory effects. on adipogenesis. Moreover, the complex extract of the traditional herbal medicines has more effect than single extracts.

• 대한한의학회지
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• 제18권2호
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• pp.43-57
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• 1997

It was claimed that the herbal medicine with the function of strengthening the body resistance exerts to enhance the immunity. And the medicine with the effect of eliminating the pathogenic factor is stated to inhibit the immune response. To evaluate the the effects of the herbal medicine on the immune response, the mice were administrated with the herbal medicine for 2 weeks. And the responses were analyzed. As the result, water extract of Radix Astragali, Fructus Psoraleae, Cortex Acanthopanacis, Semen Coicis, Herba Ecliptae, Spica Prunellae, and Radix Sophorae increased the ROI production, while Radix Tripterygia inhibited it. Phagocytic activity was increased after administration of Radix Astragal, Fructus Psoraleae, Cortex Acanthopanacis, Herba Ecliptae, Spica Prunellae and Radix Sophorae. NK cell activity was also significantly inhibited by Radix Tripterygia. Administration of Radix Astragali, Fructus Psoraleae, Cortex Acanthopanacis, Herba Ecliptae, Spica Prunellae and Semen Coicis enhanced the antibodies(hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen, while Radix Sophorae and Radix Tripterygia decreased it. Radix Sophorae and Radix Tripterygia also decreased the allogenic immune response and mixed-lymphocyte reaction. And all the experimental herbs decreased contact hypersensitivity against dinitroflurobenzene. These results show Radix Astragali, Fructus Psoraleae, Spica Prunellae, Cortex Acanthopanacis, Semen Coicis and Herba Ecliptae enhanced innate immunity, humoral and cellular immune responses. However Radix Sophorae and Radix Tripterygia exert imunosuppressive action. Also these results indicate that the medicine with the action of the strengthening the body resistance enhances the immunity. And the the some of drugs belonging to the eliminating the pathogenic factor also increase the immune responses.

• Korean Journal of Acupuncture
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• 제25권1호
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• pp.165-196
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• 2008

Objective : The purpose of this study is to observe the effects of herbal-acupuncture with Acanthopanacis cortex extract (AC-HA) at KI10 (Eumgok) on osteoporosis in ovariectomized (OVX) ddy mice. Methods : We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum creatinine, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : Tibial BMD was significantly decreased by OVX, while significantly increased by AC-HA at KI10. Serum creatinine level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Serum osteocalcin level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Tibial TBV(trabecular bone volume) was significantly decreased by OVX, while significantly increased by AC-HA at KI10. AC-HA at KI10 significantly suppressed the OVX-induced increase of GPL(growth plate length) of tibia in mice. Conclusion : These results suggest that AC-HA at KI10 has a therapeutic effect on osteoporosis in OVX mice.

• Korean Journal of Acupuncture
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• 제22권2호
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• pp.75-87
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• 2005

Objective : The purpose of this study is to observe the effect of Herbal-cupuncture with Acanthopanacis cortex Herbal-Acupuncture Solution (AC-HAS) on rheumatoid arthritis. Methods : The author performed several experimental items : it is paw thickness, weight of spleen, expression of $CD4^+,\;CD8^+,\;B220^+$ in the spleen, production of IL-6, $TNF-{\alpha}$, and examination of histology. Results : The obstain results are summarized s follows. 1. Treatment group were significantly inhibited the incidence of arthritis, hind paw edema and index of arthritis of CIA mice. 2. Treatment group were significantly decreased splenetic weight 3. The number of $CD4^+,\;CD8^+,\;B220^+$ activated cells were significantly inhibited in treatment group. 4. Treatment group were significantly decreased the amout of IL-4, $TNF-{\alpha}$ of CIA mice.. 5. Treatment group were expressed form of new bone, synoviumin and new margine in histology imperison to control group. Conclusions : Taking all these observations into account, AC-HAS injection is considered to be effective in treating rheumatoid arthritis and put to practical use in future rheumatoid arthritis clinic.

• Natural Product Sciences
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• 제13권4호
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• pp.283-288
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• 2007

Acanthopanacis Cortex (AC) has been popularly used as an herbal medicine for medical treatment of rheumatoid arthritis, insomnia, impotence and diabetes. Here, we investigated immunostimulating effects of the aqueous extract of AC on macrophage. We studied nitric oxide (NO) and tumor necrosis factor (TNF)-${\alpha}$ release in response to AC treatment, as they are important secretory products of macrophage. AC alone induce the NO and TNF-${\alpha}$ production. AC increase c-Jun NH2-terminal kinase 1/2 (JNK) and extracellular signal-regulated kinase (ERK) phosphorylation but does not p38 activation in RAW 264.7 cells. Also AC resulted in the enhanced cell-surface expression of CD80 and CD14. In addition, AC resulted in enhanced T cell-stimulatory capacity and increased T cell secretion of interferon (IFN)-gamma. After feeding with AC to mouse for 10 days, the change of $CD28^+$ and $CD40^+$ population was analyzed. AC increased $CD28^+$ population in splenocytes in vivo. These studies indicate that AC induces macrophage activation and suggest the possible use of AC in macrophage-based immunotherapies.

• 대한한의학회지
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• 제31권4호
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• pp.115-128
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• 2010

Objectives: The aim of this study was to investigate deodorizing effectsof medicinal herbs (Mume Fructus, Eriobotryae Folium, Acanthopanacis Cortex, Angelicae Dahuricae Radix) for development of a gargle solution. Methods: 1. The antimicrobial effects of medicinal herbs were evaluated with the minimal bactericidal concentration (MBC) and the change of the number of viable cells in the herb extracts(1%) for 48 hrs against P. gingivalis 2561 and Pr. intermedia ATCC 25611. 2. Deodorizing activity of each herb and Garglin $Mint^{(R)}$gainst methyl mercaptan were analyzed by gas chromatography (GC). 3. We used the malodor modeling of the salivary sediment system with a Halimeter. 4. In the preliminary clinical study, the baseline concentration of VSC in the oral cavity of each subject was measured by Halimeter. Subjects would gargle for 30 seconds with cysteine. After 4 minutes subjects would gargle for 30 seconds with Garglin and herb extracts (2%). Subsequently, concentration of VSC were measured at 0, 4, 8, 12 and 20 minutes. Results: 1. MBC of Mume Fructusfor P. gingivalis 2561 was determined to be <1% and MBCs of Eriobotryae Folium for P. gingivalis 2561 and Pr. intermedia ATCC 25611 were determined to be <2% and <1%, respectively. Mume Fructus (1%) completely suppressed the P. gingivalis cell viability from 5 hrs and Eriobotryae Folium (1%) completely suppressed the Pr. intermedia cell viability from 48 hrs. 2. In GC analysis, deodorizing activities were 91.54% with Mume Fructus, 87.97% with Eriobotryae Folium, 100% with Acanthopanacis Cortex, 72.36% with Angelicae Dahuricae Radix and 40.54% with Garglin $Mint^{(R)}$. 3. In malodor modeling of the salivary sediment system, each of the medicinal herbs had significantly inhibitory effect on malodor formation (p<0.05). 4. In the preliminary clinical study, the concentration of VSC of the herb groups was significantly lower than of the control group, but not in Garglin $Mint^{(R)}$. Conclusions: Mume Fructus, Eriobotryae Folium, Acanthopanacis Cortex and Angelicae Dahuricae Radixhave deodorizing activities and potential as an effective mouthwash against oral malodor.

• 한국식품과학회지
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• 제38권5호
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• pp.668-673
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• 2006

본 실험은 오가피열수추출물이 알레르기성 접촉 피부염을 유발한 흰쥐의 체내 지질 조성, 항산화활성, serum Ig E 수준과 표피회복능에 미치는 영향을 조사하기 위해 행해진 것으로 1-chloro-2,4-dinitrochlorobenzene(DNCB: 2.5% DNCB 1 mL로 감작, 4일 경과 후 1% ONCB 1 mL로 유발) 처리로 알레르기성 접촉 피부염이 유발된 Sprague-Dawley 수컷 흰쥐에 오가피열수추출물 (100 mg/체중 kg/day)을 경구투여 한 다음 혈장과 간의 지질조성과 항산화활성, 혈중 Ig E 농도, 표피두께변화를 관찰하여 다음과 같은 결과를 얻었다. 혈장의 total cholesterol과 LDL-cholesterol은 정상군에 비해 ACFG군에서 유의적으로 감소하여(p<0.05) 오가피 열수추출물이 지질개선에 효과적인 것으로 나타났다. 혈장과 간의 지질과산화물 함량은 정상군에 비해 CG군과 ACFG군에서 지질과산화물 함량이 유의하게 높았으며(p<0.05), ACFG군이 CG군에 비해 과산화물 함량이 감소하는 경향을 보였다. 간의 SOD효소활성은 CG군에서 정상군에 비해 유의하게 증가하였으며(p<0.05), ACFG군에서 정상군의 수준으로 유의하게 감소하였다(p<0.05). Serum 중 Ig E 수준은 정상군에 비해 CG군에서 유의하게 증가하였으며(p<0.05), ACFG군에서 CG군에 비해 정상군의 수준으로 유의하게 감소하였다(p<0.05). 표피두께는 정상군에 비해 CG군에서 표피두께가 유의적으로 증가하였으며(p< 0.05), ACFG 군에서 CG군에 비해 유의하게 감소하였다(p<0.05). 혈장과 간의 지질과산화물 함량과 표피두께의 상관계수가 각각 r=0.644(p<0.01), r=0.516(p<0.05) 로 비교적 높은 정의 상관관계를 보였다. 이상의 결과로 미루어 볼 때 정상 흰쥐에 비하여 DNCB에 의해 유도된 접촉성 피부염을 가진 흰쥐의 혈장과 간의 지질과산화물 생성이 증가되며 이로 인해 표피의 두께가 증가하고, SOD 활성이 증가하는 것으로 나타났다. 오가피열수추출물의 섭취로 알레르기성 접촉피부염으로 유발된 흰쥐의 체내 지질과산화물 생성 억제, Ig E 농도감소, 표피두께 감소되는 효과가 나타났다.

• 한국식품영양과학회지
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• 제37권4호
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• pp.512-520
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• 2008

오가피(Acanthopanacis cortex)의 유효성분 및 항산화적 특성을 조사하기 위하여 반응표면분석법에 의하여 열수추출 특성을 모니터링 하여 최적추출조건을 설정하였다. 총 추출수율의 최적조건은 추출온도 $84.28^{\circ}C$, 추출시간 3.59 hr 및 시료에 대한 용매비 18.37 mL/g이었고, 유효성분 eleutheroside E에 대한 최적조건은 $81.16^{\circ}C$, 3.56 hr, 19.06 mL/g, 총 페놀성 화합물에 대한 최적조건은 $88.45^{\circ}C$, 4.33 hr, 21.12 mL/g 등으로 각각 나타났다. 각 변수에 대한 회귀식을 도출하여 공정 인자별 오가피의 총 추출수율, 유효성분 eleutheroside E와 chlorogenic acid 함량 및 항산화적 특성에 대한 최적추출조건을 superimposing한 결과, 추출온도 $81{\sim}88^{\circ}C$, 추출시간 $3.5{\sim}4.3hr$ 및 시료에 대한 용매비 $18{\sim}21mL/g$의 범위로 각각 예측되었다. 오가피의 유효성분과 항산화적 특성에 대한 열수추출조건의 예측값은 실제값과 유사하였다.