Optimizing the Hot-water Extraction Conditions for Acanthopanacis cortex Using Response Surface Methodology

In order to examine the effective components and antioxidative characteristics of Acanthopanacis cortex, response surface methodology was used to optimize the hot water extraction process by analyzing and monitoring the extraction condition characteristics. The total extraction yield was optimized using the extraction temperature of $84.28^{\circ}C$, extraction time of 3.59 hr and 18.37 mL/g as the amount of solvent per sample. For the effective component of eleutheroside E, the optimal extraction temperature, time and amount of solvent per sample were $81.16^{\circ}C$, 3.56 hr, and 19.06 mL/g, respectively; also, for total phenolics, the optimal conditions were $88.45^{\circ}C$, 4.33 hr, and 21.12 mL/g, respectively. Regression equations were deduced for each variable and then eleutheroside E, chlorogenic acid, and antioxidative characteristics were superimposed with the optimal conditions to obtain values for each extraction process factor. The predicted results were $81{\sim}88^{\circ}C$, $3.5{\sim}4.3hr$, and $18{\sim}21mL/g$, respectively. The estimated values for the optimal extraction conditions of Acanthopanacis cortex's effective components and antioxidative characteristics were similar to the experimental results.

반응표면분석법을 이용한 오가피의 열수 추출조건 최적화

오가피(Acanthopanacis cortex)의 유효성분 및 항산화적 특성을 조사하기 위하여 반응표면분석법에 의하여 열수추출 특성을 모니터링 하여 최적추출조건을 설정하였다. 총 추출수율의 최적조건은 추출온도 $84.28^{\circ}C$, 추출시간 3.59 hr 및 시료에 대한 용매비 18.37 mL/g이었고, 유효성분 eleutheroside E에 대한 최적조건은 $81.16^{\circ}C$, 3.56 hr, 19.06 mL/g, 총 페놀성 화합물에 대한 최적조건은 $88.45^{\circ}C$, 4.33 hr, 21.12 mL/g 등으로 각각 나타났다. 각 변수에 대한 회귀식을 도출하여 공정 인자별 오가피의 총 추출수율, 유효성분 eleutheroside E와 chlorogenic acid 함량 및 항산화적 특성에 대한 최적추출조건을 superimposing한 결과, 추출온도 $81{\sim}88^{\circ}C$, 추출시간 $3.5{\sim}4.3hr$ 및 시료에 대한 용매비 $18{\sim}21mL/g$의 범위로 각각 예측되었다. 오가피의 유효성분과 항산화적 특성에 대한 열수추출조건의 예측값은 실제값과 유사하였다.