• Korean Journal of Microbiology
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• v.51 no.3
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• pp.187-193
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• 2015

Candida (also known as Pseudozyma) antarctica lipase B (CAL-B) has been intensely studied in academic and industrial fields. However, the research related to its homologous enzymes has been rarely reported. In the current investigation, protein sequence similarity search of CAL-B has been conducted and six homologous protein sequences were identified. After the syntheses of their codon-optimized genes, the synthetic genes have been cloned into a periplasmic expression vector to express in Escherichia coli. Among six homologous sequences, four sequences were successfully expressed in E. coli. The hydrolytic activities of the expressed proteins towards 4-nitrophenyl acetate and 4-nitrophenyl butyrate were measured and compared with those of CAL-B to identify whether the expressed proteins work as a hydrolase. It has been revealed that the expressed proteins can hydrolyze the substrates and the specific activities were determined as $(1.3-30){\times}10^{-2}{\mu}mol/min/mg$, which are lower than those of CAL-B. Among these homologous enzymes, Pseudozyma hubeiensis SY62 exhibits the comparable enantioselectivity to that of CAL-B towards the hydrolysis of (${\pm}$)-1-phenylethyl acetate.

• 한국산학경영학회:학술대회논문집
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• 2006.06a
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• pp.171-190
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• 2006

Toyota 2005 annual report said Toyota has six consecutive fiscal year positive revenues and record breaking revenues during recent four consecutive years. Net profit is 11% grower than last year. (\11,000,000,000,000). In the spotlight they have record breaking net profit every year, their strongest point is their unique production system which come up with MUDA(waste) free and ongoing improvement activity. Their basic production philosophy is value added through improve production efficiency which can be possible by eliminating MUDA(waste). This basic philosophy's key points are "Just in Time" and "Automation with human". Recently, Korean manufacturing companies and service companies have a growing interest in Toyota Production System. They try to find ways for appling Toyota system to their companies. Through observing Toyota production theory, understand Toyota Production System, Through studying practical application in industrial fields, examine application possibility for Korean industry and more detailed researches are required.

• Journal of Arbitration Studies
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• v.30 no.3
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• pp.47-66
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• 2020

Due to the division of China's departmental laws, the disconnect between theory and practice, and the influence of traditional academic thinking on the understanding of the knowledge structure of arbitration legal talents in practice, the construction of law school colleges, teaching teams, and research centers mostly revolves around departmental laws, tearing the connection of the arbitration legal system. The student-centered, process-guaranteed, and result-oriented arbitration master of law training model is "virtualized," the shaping of arbitration professionalism is ignored, the coverage of practical teaching is narrowed, and the arbitration legal profession is mostly formalized. The prevalence of specialized curriculum systems shortage, single faculty, formalized practical teaching, outdated curriculum settings, unsuitable curriculum system design for development, and inaccurate professional curriculum standards and positioning renders it difficult to integrate the "Belt and Road." The cutting-edge, the latest research results, and practical experience cannot reflect the connotation, goals, and requirements of "Entrepreneurship" education, as well as arbitral issues such as the ineffective monitoring of practical education and the inconsistent evaluation of standards and scales. Under the background of the "Belt and Road," based on system theory and practice and through training goals that innovate and initiate organizational form, activity content, management characteristics, assessment and support conditions, etc., the arbitration law teaching curriculum system is gradually improved and integrated. Through the establishment of a "Belt and Road" arbitration case file database and other measures, a complete arbitration law theory and practice teaching guarantee system has been established. Third parties are introduced, arbitration law experimental modules are developed, students are guided how to discover new knowledge, new contents are mastered, solidarity, cooperation, and problem-solving capabilities are cultivated in the practice of the "Belt and Road," and quality education, vocational education, and innovation education are organically integrated. In order to implement the requirements of arbitration law education, innovation development and collaborative management of arbitration law teaching practice base should be cultivated, thus giving full play to the effect of collaborative education between universities and arbitration institutions.

• The Journal of the Korea Contents Association
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• v.20 no.6
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• pp.695-704
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• 2020

Recently, There has been an increasing of utilization IT, and studies have been conducted on predicting learning results. In this study, Learning activity data were collected that could affect learning outcomes by using learning analysis. The survey was conducted at a university in South Chung-Cheong Province from October to December 2018, with 1,062 students taking part in the survey. First, A Hierarchical regression analysis was conducted by organizing a model of individual, academic, and behavioral factors for learning results to ensure the validity of predictors in machine learning. The model of hierarchical regression was significant, and the explanatory power (R²) was shown to increase step by step, so the variables injected were appropriate. In addition, The linear regression analysis method of machine learning was used to determine how predictable learning outcomes are, and its error rate was collected at about 8.4%.

• Molecules and Cells
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• v.39 no.11
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• pp.821-826
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• 2016

The ${\beta}$-catenin functions as an adhesion molecule and a component of the Wnt signaling pathway. In the absence of the Wnt ligand, ${\beta}$-catenin is constantly phosphorylated, which designates it for degradation by the APC complex. This process is one of the key regulatory mechanisms of ${\beta}$-catenin. The level of ${\beta}$-catenin is also controlled by the E3 ubiquitin protein ligase SIAH-1 via a phosphorylation-independent degradation pathway. Similar to ${\beta}$-catenin, STAT3 is responsible for various cellular processes, such as survival, proliferation, and differentiation. However, little is known about how these molecules work together to regulate diverse cellular processes. In this study, we investigated the regulatory relationship between STAT3 and ${\beta}$-catenin in HEK293T cells. To our knowledge, this is the first study to report that ${\beta}$-catenin-TCF-4 transcriptional activity was suppressed by phosphorylated STAT3; furthermore, STAT3 inactivation abolished this effect and elevated activated ${\beta}$-catenin levels. STAT3 also showed a strong interaction with SIAH-1, a regulator of active ${\beta}$-catenin via degradation, which stabilized SIAH-1 and increased its interaction with ${\beta}$-catenin. These results suggest that activated STAT3 regulates active ${\beta}$-catenin protein levels via stabilization of SIAH-1 and the subsequent ubiquitin-dependent proteasomal degradation of ${\beta}$-catenin in HEK293T cells.

• Korean Journal of Occupational Health Nursing
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• v.12 no.1
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• pp.71-79
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• 2003

Purpose: The purpose of this study was to describe the status of maternity protection in the industries for the development of educational database used by occupational health nurses (OHNs). Method: 100 OHNs were purposively sampled and they were charged in the occupational health manager of their own industries. Data were collected by the postal mail, the response rate was 53.0%, and analysis rate was 43.0%. Result: The results were as follows; 1. Most of subjects responded there was the institutionalization of a special holiday for woman workers (97.7%) and 90 days maternity leave (95.4%). Otherwise, the numbers of industry were smaller in the case of providing the breast-feeding time, temporary rest from office for child raring, restriction of hazard job, night job, and over time. The application rates were lower than the rate of institutionalization of all maternity protection items. 2. There were significant differences in institutionalization of maternity protection by the activity of the trade union. And the numbers of the industry applying the maternity protection were significantly different by the area and the types of industry. 3. Most OHNs responded 17 subjects were needed to the maternity protection education for women workers. Conclusion: It would be needed to develop the maternity protection education database in base of the status of maternity protection in the industries and the need of maternity protection education. The role of OHNs for maternity protection was expected to activate.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.7
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• pp.4418-4423
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• 2015

This thesis urges the change of the education system of popular music for globalization of K-POP in a longer point of view Korean popular music has developed not only musically but also sound quality itself. Moreover it became popular in a global scale in the name of K-POP. However, it is still limited to idol music, and numerous issues related to the lack of musical talent are emerging Even though the routine of birth and death of several idol groups and singers are still repeated. Recently, the skills of artists enhanced due to audition TV shows but still it's not enough. Under this situation, it is not appropriate to rely on audition TV shows and Entertainment Management companies for picking up talented artists. Therefore, like Classic or Traditional music, schools concentrated for popular music must be founded. If this become enabled, young students going to entertainment companies or to unqualified but expensive private institutes will disappear. Moreover, it can prevent students from lacking behind in their academic activity. In addition, a reformation should be done education colleges for raising popular music educators

• Korean Business Review
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• v.21 no.2
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• pp.79-97
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• 2008

Between different organization, they creat competitiveness from the result of their own manufacturing and sales activity especially the manufacturing companies. This paper will focus on the spot location strategy of korea company which have oversea business and find out the relationship between the spot location strategy and the resources or competitive ability of this kind of companies. Each company has their own way to realize their overseas business expansion and creat their competitiveness in their international business. At the same time, they also have their own way to share their resources and their competitive ability. All this 3 kinds of strategies are important and the combination of them is more important for companies that have overseas business. To examine the relationship between the spot location, the resources and the competitive ability strategies, this paper developed two hypotheses and tested them. To test the hypotheses, this paper conducted a mail survey to operations managers of overseas companies in Korea and collected 102 usable responses. To simplify the responses, this paper used cluster analysis and found 3groups of spot location strategy, 2 groups of resources strategy and 2 groups of competitive abilities strategy. This paper also investigated interdependence and performance differences between these groups. From the analyses, this paper found the following results: It is important for a overseas company to make their spot location strategy to be fit for their resources strategy and competitive abilities strategy and a perfect spot location strategy can make the company gain a advantage of high level in most of time.

• The Journal of Korean Society for School & Community Health Education
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• v.17 no.1
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• pp.101-112
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• 2016

Objectives: The purpose of this study was to examine the association between participation in physical activities (PA) and subjective well-being. Methods: We analyzed the data from Korean Youth Risk Behavior Web-based Survey, 2013 containing 36,655 and 35,780 for male and female students respectively. Study variables included PA, participation in physical education class, participation in team sports and subjective well-being. Control variables were school type, socio-economic status, level of parent education, living with parent, academic performance, delinquent behaviors, depression and perceived health. For this study, descriptive analysis, Chi-square test, and multivariate logistic regression analysis were performed. Results: Among boys, after adjusting for control variables, PA (past 7 days 0 time reference group vs. over 4 times: OR 1.306), physical education class (past 7 days 0 time reference group vs. over 3 times: OR 1.140), and team sports (no team reference group vs. 2 team: OR 1.206) were associated with subjective well-being. Among girls, control variables were adjusted. PA (past 7 days 0 time reference group vs over 4 times: OR 1.244), physical education class (past 7 days 0 time reference group vs. over 3 times: OR 1.161), and team sports (no team reference group vs. 2 team: OR 1.181) were associated with subjective well-being. Conclusions: Participation in physical activities may help adolescent promote their life satisfaction. Thus, education experts should develop various exercise curriculums to encourage participation in physical activities.

• Journal of the Korean Society of School Health
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• v.29 no.2
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• pp.90-97
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• 2016

Purpose: The purpose of this study was to investigate the relationship between sleep timing and depressive mood in Korean adolescents. Methods: The study analyzed the data from the 2007~2015 Korea Youth Risk Behavior Web-based Survey. A total of 541,693 students in grades 7~12 were included in the final analysis. Multivariable logistic regression was used to examine their sleep timing and depressive mood, adjusted for sex, grade, region, socioeconomic status, academic performance, alcohol, smoking and physical activity. Sleep duration and sleep quality were also included in our model to identify whether or not the effect of sleep timing on depression is mediated by sleep duration or sleep quality. Results: The prevalence of depressive mood was 32.7% and the mean sleep timing was 12:13 AM. After adjustment for eligible covariates, the association between sleep timing and depressive mood showed a J-shaped curve. Adolescents who slept at 8 pm~10 pm were 39% more likely to be depressive (OR = 1.39, 95% CI 1.30~1.40) and at 3 am~ 4 am were 67% more likely to be depressive (OR=1.67, 95% CI 1.64~1.70) than adolescents who slept at 11 pm~12 am. These associations persisted after being adjusted for sleep duration and sleep quality. Conclusion: Sleep timing was related to depression in adolescents, independent of sleep duration and sleep quality. It appears that there is a certain sleep timing beneficial to mental health of adolescents.